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In vitro induction of resistance to metronidazole, and analysis of mutations in rdxA and frxA genes from Helicobacter pylori iso
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Susceptible and resistant strains after the in vitro induction of resistance were examined to evaluate mutations of the rdxA and frxA genes by sequence analysis.
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After the in vitro induction of resistance, analysis revealed that two and four susceptible strains developed resistance when cultured with 0.3?μg/ml and 0.6?μg/ml of metronidazole, respectively.
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The results after the in vitro induction of resistance to metronidazole suggested the presence of additional metronidazole resistance mechanisms, other than mutations of the rdxA and/or frxA genes.
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