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改良法     
相关语句
  improved method
    An Improved Method for Detecting Angiotensin Converting Enzyme(ACE) Gene Insert/Delete(I/D) Polymorphism Using Dimethyl Sulphoxide
    血管紧张素转换酶基因插入/缺失多态性改良法与经典法检测比较
短句来源
    The improved method was studied in respects of 5 fixation fluids and time and temperature of silver impregnation.
    应用浸银改良法对固定液及浸银温度、时间等影响因素作了实验研究以及与Grimelius嗜银法和M -H亲银法作了比较研究。
短句来源
    Methods:The ACE genotypes of 168 cases were measured by classic Rigat method and an improved method by adding 5% dimethyl sulphoxide (DMSO) respectively,and the results were compared.
    方法 :用在 PCR反应系统中加入 5 %的二甲基亚砜 (DMSO)的改良法检测了 16 8例健康人群 ACE基因型 ,并与经典 Rigat方法进行比较。
短句来源
    An improved method of isolating and identifying rat hepatic stellate cells
    改良法大鼠肝星状细胞的分离培养及鉴定
短句来源
    An improved method for the atrioventricular node ischemia-reperfusion in rabbit heart.
    改良法创建兔心房室结缺血再灌注损伤模型
短句来源
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  modified method
    Experimental study on differentiation of bone marrow stromal cells to osteoblasts by modified method
    改良法培养骨髓基质干细胞向成骨细胞分化的实验研究
短句来源
    Experimental study of rat myoblast cultured with an in vitro modified method
    改良法体外培养大鼠成肌细胞的实验研究
短句来源
    As compared to that in reference, the sensitivity of this modified method was increased by 1.9 fold.
    此改良法建立了测定大鼠脑组织GABA-T活性的微量紫外分光比色法,灵敏度比国外同类方法提高1.9倍。
短句来源
    Conclusion The modified method is simple and convenient.
    结论本改良法是一种较方便而且有效的培养法。
短句来源
    Results: The globin chains can be well separated by this modified method and the electrophoresis time has been shorted down (from 6 hours to 2 hours ).
    结果 :该改良法能使凝胶聚合时间缩短、一次电泳时间从 6h缩短为 2h ,HbF中各珠蛋白链得到良好分离 ,电泳条带清晰 ,无拖尾现象 ;
短句来源
更多       
  an improved method
    An Improved Method for Detecting Angiotensin Converting Enzyme(ACE) Gene Insert/Delete(I/D) Polymorphism Using Dimethyl Sulphoxide
    血管紧张素转换酶基因插入/缺失多态性改良法与经典法检测比较
短句来源
    Methods:The ACE genotypes of 168 cases were measured by classic Rigat method and an improved method by adding 5% dimethyl sulphoxide (DMSO) respectively,and the results were compared.
    方法 :用在 PCR反应系统中加入 5 %的二甲基亚砜 (DMSO)的改良法检测了 16 8例健康人群 ACE基因型 ,并与经典 Rigat方法进行比较。
短句来源
    An improved method of isolating and identifying rat hepatic stellate cells
    改良法大鼠肝星状细胞的分离培养及鉴定
短句来源
    An improved method for the atrioventricular node ischemia-reperfusion in rabbit heart.
    改良法创建兔心房室结缺血再灌注损伤模型
短句来源
  “改良法”译为未确定词的双语例句
    An Improved Gel “Disc” Electrophoresis of Amniotic Fluid Cholinesterase and Its Applied Value of Prenatal Diagnosis for Neural Tube Defects
    羊水胆碱酯酶凝胶圆盘电泳改良法及其产前诊断神经管缺损的应用
短句来源
    APPLICATION OF MODIFIED SPA-ELISA TO TESTING THYMOSIN A GENE EXPRESSION PRODUCT
    SPA-ELISA改良法在检测胸腺素α_1基因表达产物中的应用
短句来源
    Focal cerebral ischemia models were prepared by Logna et al modified thread occlusion method, and the functional evaluation was performed after the animals were awake for 2 hours to select the rats whose nervous function reached grade 2 or above.
    采用Logna等改良法复制大脑中动脉血栓模型,动物清醒2h后进行功能评价,动物神经功能达到2级的纳入实验。
短句来源
    ④reformed filter method: The masses were trypsinized with 1 mL digestive, blown for 30 s, swayed for 3-5 minutes at 37 ℃ tank. 1 mL complete medium was added to stop digestion, and then laid at room temperature for 1-2 minutes. After filtering with 150 wells filter with 5 cm diameter, filtered liquid was centrifuged for 5 minutes at 1 000 r/min.
    ④滤网过滤改良法:将剪碎的组织块加入1mL消化液,吹打30s,37℃水溶箱中轻摇3~5min,加入完全培养基1mL终止消化,室温下静置1.0~2.0min,用直径5cm、孔径为150目滤网过滤后,将过滤液以1000r/min离心5min;
短句来源
    ③Cell cycle and proliferation index (PI): On the 3rd day, the PI of cells isolated by reformed filter method was 54.12%, which was the highest. While the PI of cells isolated by single cell suspension method was 53.52% and tissue cultural method was 53.15%. There was no significant difference among the three methods (P > 0.05), which indicated that cells isolated by different methods had the same cell cycle.
    ③细胞周期检测与增殖指数:培养第3天,滤网过滤改良法所得的鼠胚胎成纤维细胞增殖指数最高,达到54.12%,单细胞悬液法、组织块培养法细胞增殖指数分别为53.52%和53.15%,3种方法差异无显著性意义(P>0.05),表明其细胞周期基本相同。
短句来源
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  improved method
We used an improved method of increasing ICP to establish a stable BD model.
      
An improved method of photometric determination of cyclodextrin glucanotransferase activity
      
An improved method for assaying of the total endodepolymerase activity of pectinases has been developed.
      
On the basis of [1] an improved method was developed which, within the framework of the model of an ideal gas, allows one to calculate the flow in ejector nozzles without a limit on the coefficient of ejection.
      
The use of an improved method of numerical solution [3] has made it possible to advance into the region of large temperature differences.
      
更多          
  modified method
Modified Method for the Determination of Grain Amylase Activity by the Falling Number
      
Easily verifiable necessary and sufficient conditions for the absolute stability of two-dimensional time-varying systems are derived with a modified method of comparison of two-dimensional systems.
      
The data of EPR spectroscopy and spectrophotometric titration of copper sites showed that only a modified method based on the use of bathocuproine as a chelator for type 2 copper yielded laccase derivatives completely free from type 2 copper.
      
The applicability limits of the modified method are indicated.
      
A modified method of the transporting trajectory is applied in order to find the optimal transfer under the linear constraints on the thrust direction.
      
更多          
  an improved method
We used an improved method of increasing ICP to establish a stable BD model.
      
An improved method of photometric determination of cyclodextrin glucanotransferase activity
      
An improved method for assaying of the total endodepolymerase activity of pectinases has been developed.
      
On the basis of [1] an improved method was developed which, within the framework of the model of an ideal gas, allows one to calculate the flow in ejector nozzles without a limit on the coefficient of ejection.
      
The use of an improved method of numerical solution [3] has made it possible to advance into the region of large temperature differences.
      
更多          


The left ventricular myocardial catecholamine(CA), glycogen and pgosphorylase were histochemically studied in 35 rabbits which were divided into 3 groups---normal control, sham operation and hemorrhagic shock.

本文以35只家兔分三组对失血性休克2小时动物心肌进行了三种方法的组织化学观察。结果看到:失血性休克发生后,心肌儿茶酚胺(CA)含量明显减弱以至耗竭(乙醛酸诱发CA荧光方法)、心肌糖原(PAS反应)和磷酸化酶(磷酸化酶改良法)大量脱失,部分心肌细胞明显肿胀。而假手术对上述指标影响很轻。结果说明,失血性休克对心肌组织化学影响很大,导致心脏交感神经功能低下,心肌代谢紊乱,可能是造成本文绝大部分(13/14)休克动物死亡的主要因素。

The plasmid containing poliovirus cDNA (sabin I) was amplified in E. coli HB 101 strainand extracted with different methods. AGMK cells and 2 BS cells were transfected with thepurified DNA. The result shows that the plasmid containing poliovirus cDNA (sabin I) extractedwith modified CsCl method is capable of preserving the function of complete nucleotide sequence,indicating the virus being recovered successfully. The biological properties and immunity ofrecovered virus are the same as those of poliovirus sabin...

The plasmid containing poliovirus cDNA (sabin I) was amplified in E. coli HB 101 strainand extracted with different methods. AGMK cells and 2 BS cells were transfected with thepurified DNA. The result shows that the plasmid containing poliovirus cDNA (sabin I) extractedwith modified CsCl method is capable of preserving the function of complete nucleotide sequence,indicating the virus being recovered successfully. The biological properties and immunity ofrecovered virus are the same as those of poliovirus sabin I strain.

用大肠杆菌HB 101株扩增了含有poliovirus sabin 1型全序列cDNA plasmid。以不同的方法进行提取 polio cDNA(sabin 1)plasmid,转染 2 BS细胞,非洲绿猴肾细胞(AGMK)。结果表明:氯化铯改良法提取之polio cDNA,保持了序列完整功能,转染细胞成功。对恢复病毒进行了生物学性质与抗原性的鉴定,结果与poliovirus sabin 1型相同。

The histochemical methods of aerobic oxidative key enzyme isocitratedehydrogenase,including NADP~+-and NAD~+-dependent isocitrate dehydrogenase(T-IDH and D-IDH)and of glycolytic key enzyme hexokinase(HK)were studied in thenasopharyngeal epithelia and around tissues of 50 normal rats.It was shown thatthe results of the modified methods of T-IDH,D-IDH and HK in three types of thenasopharyngeal epithelia were more reliable than that of Bancroft's and meijer'smedia(without phenazine methosulfate),the activity of...

The histochemical methods of aerobic oxidative key enzyme isocitratedehydrogenase,including NADP~+-and NAD~+-dependent isocitrate dehydrogenase(T-IDH and D-IDH)and of glycolytic key enzyme hexokinase(HK)were studied in thenasopharyngeal epithelia and around tissues of 50 normal rats.It was shown thatthe results of the modified methods of T-IDH,D-IDH and HK in three types of thenasopharyngeal epithelia were more reliable than that of Bancroft's and meijer'smedia(without phenazine methosulfate),the activity of the three enzymes demonstratedwith modified media was stronger than that with Reider's and Lawrence's.Theimportance of several factors which affect the histochemical reaction of coenzyme--dependent dehydrogenases was also discussed.

本文用50只健康成年大鼠的鼻咽上皮及其周围组织,研究了三羧酸循环中关键酶需辅酶Ⅱ(NADP~+)与辅酶Ⅰ(NAD~+)的异柠檬酸脱氢酶(T-IDH 与 DH-IDH)和糖酵解关键酶己糖激酶(HK)的组化方法。结果表明,采用本改良法显示 T-IDH、D-IDH 和 HK,比不含酚嗪硫酸甲酯(PMS)的 Bancroft 法和 Meijer 法可靠,各种酶的活性较 Reider 法和 Lawrence 法显示的为强。文中讨论了影响需辅酶脱氢酶组化反应的几个重要因素。

 
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