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药用基因
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     Progress in the Functional Gene Research of Medicinal Plants
     药用植物功能基因
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     4 new genes were obtained.
     U基因
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     The Gene of All Fears
     害怕的基因
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     Application of gene chip technology to medicinal plant researches
     基因芯片技术在药用植物研究中的应用
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     , or R.
     、药用大黄R.
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At present,gene technology used in the area of traditional Chinese medicine(TCM)mostly lies in the fingerprinting of gene,which aims at identifying Chinese medicinal materials,while rarely dealing with the studies on the genes of drug targets and the reaction of toxicity and side effects and officinal genes as well as active protein peptides.This article presents the roles that gene technology could play in the above-mentioned studies and development and application as well as the application in the studies...

At present,gene technology used in the area of traditional Chinese medicine(TCM)mostly lies in the fingerprinting of gene,which aims at identifying Chinese medicinal materials,while rarely dealing with the studies on the genes of drug targets and the reaction of toxicity and side effects and officinal genes as well as active protein peptides.This article presents the roles that gene technology could play in the above-mentioned studies and development and application as well as the application in the studies of the modernization of TCM.And it puts forward some new ideas.

目前,基因技术在中药领域应用较多的是基因指纹图谱,主要针对药材鉴定,而在药靶基因、毒副反应基因以及药用基因与活性蛋白/肽类等方面的研究则较少涉及。本文介绍了基因技术在上述中药研究与开发应用领域的作用。同时提出了一些新见解。

AIM: To construct a high quality cDNA expression library from the venom of Chinese Cobra. METHODS: Total RNA and purified mRNA were extracted by using ”single step method” and by chromatography on oligo (dT) cellulose. The cDNA was synthesized through reverse transcription. After cDNA size fractionation was purified by chromatography column, the double cDNA was ligated to pSPORT1 plasmid vector. RESULTS: The directional library was confirmed to be about 2×10 5 independent clones in which the percentage of...

AIM: To construct a high quality cDNA expression library from the venom of Chinese Cobra. METHODS: Total RNA and purified mRNA were extracted by using ”single step method” and by chromatography on oligo (dT) cellulose. The cDNA was synthesized through reverse transcription. After cDNA size fractionation was purified by chromatography column, the double cDNA was ligated to pSPORT1 plasmid vector. RESULTS: The directional library was confirmed to be about 2×10 5 independent clones in which the percentage of recombinant clones was 97% and the whole length cDNA of CTX 3 and PLA2 gene were obtained by PCR amplification. CONCLUSION: The constructed cDNA library can be used for further screening and cloning of new protease inhibitor gene in Chinese cobra.

目的 :为研究开发眼镜蛇毒药用基因工程产品 ,筛选克隆及表达相关药用功能基因 ,构建中华眼镜蛇毒腺cDNA表达文库 .方法 :一步法提取总RNA ,Oligo(dT)纤维素层析柱纯化mRNA ,逆转录PCR合成双链cDNA ,分级分离除去小片段后 ,收集大于 5 0 0bp的cDNA片段 ,取 1 0ngcDNA与质粒载体pSPORT1连接、转化 .结果 :获得克隆总数为 2× 1 0 5的眼镜蛇毒腺cDNA表达文库 ,重组率 97% .利用PCR技术从该文库扩增了心脏毒素 3(CTX 3)和磷脂酶A2 (PLA2 )基因的cD NA .通过对文库克隆的序列测定和初步生物信息学分析 ,获得 2 4个中华眼镜蛇毒腺EST序列 .结论 :所建立的毒腺cDNA表达文库质量较高 ,可用于进一步筛选、克隆蛇毒药用功能新基因

 
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