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组织与胚胎学
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  histology and embryology
     METHODS: This experiment was conducted at the Laboratory of Pathopsychology and Laboratory of Histology and Embryology, Harbin Medical University from December 2004 to July 2005. Wistar rats of either gender, with body mass of 100 to 120 g, were used in this experiment.
     方法:实验于2004-12/2005-07在哈尔滨医科大学病理生理学实验室和组织与胚胎学实验室完成。 实验选用Wistar大鼠,雌雄不限,体质量100~120g。
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     METHODS:The experiment was performed in the Department of Histology and Embryology, JinZhou Medical College from June 2003 to June 2004. A total of 24 Kunming mice were bred together.
     方法:实验于2003-06/2004-06在锦州医学院组织与胚胎学教研室完成。 实验选用普通级昆明系小白鼠24只,同笼饲养。
短句来源
     METHODS: The experiment was performed in the Department of Histology and Embryology, Jinzhou Medical College from March 2004 to March 2005. Twenty-four Kunming mice (the ratio of female and male was 1:3) with the body mass ranged 25-30 g were bred together.
     方法:实验于2004-03/2005-03在锦州医学院组织与胚胎学教研室完成。 取成年健康昆明系小白鼠24只,体质量25~30g,雌雄(3∶1)同窝饲养。
短句来源
     METHODS: The experiment was performed in the Department of Histology and Embryology, Jinzhou Medical College from May 2005 to May 2006.①A total of 24 Kunming adult mice of either gender were bred together, weighed 25-30 g. The time that pessary fell off was termed as embryonic 0 day.
     方法:实验于2005-05/2006-05在锦州医学院组织与胚胎学教研室完成。 ①取成年健康昆明系小白鼠24只,体质量25~30g,雌、雄(1∶1)同窝饲养,见雌鼠阴道栓出现计为胚龄0d。
短句来源
     SETTING: Department of Histology and Embryology, School of Basic Medical Science, Jilin University.
     单位:吉林大学基础医学院组织与胚胎学教研室。
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  “组织与胚胎学”译为未确定词的双语例句
     SETTING: Second Clinical Medical College , Jilin University; Department of Histology & Embryology, School of Basic Medical Science, Jilin University.
     单位:吉林大学第二临床医学院,吉林大学基础医学院组织与胚胎学教研室。
短句来源
     MATERIALS: This experiment was conducted at the Department of Histology & Embryology, School of Basic Medical Science, Jilin University from October 2004 to October 2005. The rat amniotic epithelial tissue was mechanically peeled from an embryonic 12 to 14 days Wistar rats.
     材料:实验于2004-10/2005-10在吉林大学基础医学院组织与胚胎学教研室完成。 选取清洁级孕12~14d的Wistar大鼠1只,将分离出的羊膜上皮细胞用于实验。
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     Organization and Innovation
     组织创新
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     Organization and Power
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     and D.
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  histology and embryology
Human gross anatomy of the head and neck with special emphasis on the eye and orbit; histology and embryology of the eye and associated structures.
      
The general research laboratory contains some general purpose equipment for microbiology, histology and Embryology study.
      


ATM: To investigate the mRNA expression of ion channel in bone marrow mesenchymal stem cells (MSCs) cultured in vitro before and after differentiation into cardiomyocytes, so that we can further probe into the effect of ion channel in the proliferation of MSCs and differentiation into cardiomyocytes.METHODS: This experiment was conducted at the Laboratory of Pathopsychology and Laboratory of Histology and Embryology, Harbin Medical University from December 2004 to July 2005. Wistar rats of either gender, with...

ATM: To investigate the mRNA expression of ion channel in bone marrow mesenchymal stem cells (MSCs) cultured in vitro before and after differentiation into cardiomyocytes, so that we can further probe into the effect of ion channel in the proliferation of MSCs and differentiation into cardiomyocytes.METHODS: This experiment was conducted at the Laboratory of Pathopsychology and Laboratory of Histology and Embryology, Harbin Medical University from December 2004 to July 2005. Wistar rats of either gender, with body mass of 100 to 120 g, were used in this experiment. MSCs were isolated, cultured and purified, and MSCs of the third generation were labeled with DAPI (5 mg/L). MSCs were induced with 10 μmol/L 5-azacytidine to differentiate into cardiomyocytes. The expression of cellular troponin was detected by immunofluorescent staining 4 weeks after induction. Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of α1c,α1D,α1G,α1H,α1S subunit of calcium channel and ERG,IK1,KVLQT1 of potassium channels and SCN5A of sodium channel. RESULTS: ①The expression of specific treponin of cardiomyocytes could be seen 4 weeks after the induction of 5- azacytidine. ② The results of RT-PCR showed that the mRNA of α1c,α1D expressed by differentiated cells for L-type calcium channel , 1G subunit for T-type calcium channel, ERG-1,Kir2.1,KVLQT-1 for potassium channels and SCN5A for sodium channel were similar to the mature cytomyocytes. ③ The expression of α1c subunit , and ERG-1 and KVLQT-1 of potassium channels could also be found in the MSCs before induction and differentiation. CONCLUSION: MSCs could be induced and differentiated into cardiomyocytes . The mRNA of some ion channels, which produced action potential, was expressed in the differentiated cells. It is further demonstrated that BMCs could be used as seed cells of cardiomyocyte transplantation. The expression of L-type calcium channel, ERG-1 and KVLQT1 of potassium channels could also be observed in the undifferentiated BMCs, presuming that these ion channels might have important role in the proliferation and differentiation into cardiomyocytes of MSCs.

目的:观察体外诱导骨髓间充质干细胞向心肌细胞分化前后某些离子通道mRNA表达的变化,以进一步探讨离子通道在骨髓间充质干细胞增殖和向心肌细胞分化中的作用。方法:实验于2004-12/2005-07在哈尔滨医科大学病理生理学实验室和组织与胚胎学实验室完成。实验选用Wistar大鼠,雌雄不限,体质量100~120g。分离、培养和纯化大鼠骨髓间充质干细胞,以DAPI(5mg/L)标记第三代的骨髓间充质干细胞后,采用10μmol/L5-氮胞苷诱导,于诱导后第4周做细胞免疫荧光化学检测细胞肌钙蛋白的表达。采用反转录聚合酶链反应技术检测诱导分化前后钙通道的α1c,α1D,α1G,α1H,α1S亚基;钾通道:ERG,IK1,KvLQT1和钠通道SCN5AmRNA的表达结果:①5-氮胞苷诱导后第4周细胞可见心肌细胞特异性肌钙蛋白的表达。②反转录聚合酶链反应结果显示分化后的细胞表达L-型钙通道的α1c,α1D,T-型钙通道的1G亚基和钾通道:ERG-1,Kir2.1,KvLQT-1和钠通道SCN5AmRNA的表达,与成熟心肌细胞相似。③在诱导分化前的骨髓间充质干细胞也发现了L-型钙通道的α1c亚基,ERG-1和KvL...

目的:观察体外诱导骨髓间充质干细胞向心肌细胞分化前后某些离子通道mRNA表达的变化,以进一步探讨离子通道在骨髓间充质干细胞增殖和向心肌细胞分化中的作用。方法:实验于2004-12/2005-07在哈尔滨医科大学病理生理学实验室和组织与胚胎学实验室完成。实验选用Wistar大鼠,雌雄不限,体质量100~120g。分离、培养和纯化大鼠骨髓间充质干细胞,以DAPI(5mg/L)标记第三代的骨髓间充质干细胞后,采用10μmol/L5-氮胞苷诱导,于诱导后第4周做细胞免疫荧光化学检测细胞肌钙蛋白的表达。采用反转录聚合酶链反应技术检测诱导分化前后钙通道的α1c,α1D,α1G,α1H,α1S亚基;钾通道:ERG,IK1,KvLQT1和钠通道SCN5AmRNA的表达结果:①5-氮胞苷诱导后第4周细胞可见心肌细胞特异性肌钙蛋白的表达。②反转录聚合酶链反应结果显示分化后的细胞表达L-型钙通道的α1c,α1D,T-型钙通道的1G亚基和钾通道:ERG-1,Kir2.1,KvLQT-1和钠通道SCN5AmRNA的表达,与成熟心肌细胞相似。③在诱导分化前的骨髓间充质干细胞也发现了L-型钙通道的α1c亚基,ERG-1和KvLQT-1钾通道的表达。结论:骨髓间充质干细胞可被诱导分化为心肌样细胞,分化后的细胞表达形成动作电位的相关离子通道基因,进一步表明骨髓间充质干细胞可作为心肌细胞移植的种子细胞。实验观察到未分化的骨髓间充质干细胞也有L-型钙通道,ERG-1和KvLQT1钾通道的表达,推测这些离子通道可能在骨髓间充质干细胞的增殖和向心肌细胞分化中具有重要的作用。

BACKGROUND: It has been suggested that amniotic epithelial cells (AECs) express almost all of the markers of neural cell and secret a lot of neurotrophic factors and neurotransmitters. If AECs could substitute neural cells, its neurotrophic effect will bring promising prospect in treating neuron injuries and degenerative neural disease. OBJECTIVE: To detect specific proteins of neural cells in rat's cultured AECs. DESIGN: Repeated measurement design. SETTING: Second Clinical Medical College , Jilin University;...

BACKGROUND: It has been suggested that amniotic epithelial cells (AECs) express almost all of the markers of neural cell and secret a lot of neurotrophic factors and neurotransmitters. If AECs could substitute neural cells, its neurotrophic effect will bring promising prospect in treating neuron injuries and degenerative neural disease. OBJECTIVE: To detect specific proteins of neural cells in rat's cultured AECs. DESIGN: Repeated measurement design. SETTING: Second Clinical Medical College , Jilin University; Department of Histology & Embryology, School of Basic Medical Science, Jilin University. MATERIALS: This experiment was conducted at the Department of Histology & Embryology, School of Basic Medical Science, Jilin University from October 2004 to October 2005. The rat amniotic epithelial tissue was mechanically peeled from an embryonic 12 to 14 days Wistar rats. Mouse anti Nestin was purchased from Chemicon Co., and anti-ChAT rabbit anti-NSE and anti-NT-3 antibodies from Wuhan Boshide Company. Mouse anti-Musashi antibody was donated by Pro.Okano. METHODS: AECs were dissociated and purified from the amnion of pregnancy 12-14 day rats. AECs were treated with trypsin for 5 minutes, then cultured in DMEM/F12 medium at a humidified atmosphere of 0.05 volume fraction of CO2 in air at 37 ℃. Cells were inoculated at a concentration of 5×109 cells/L in culture flask. After 3 days, cells were inoculated onto poly-lysine-treated 35 mm culture Petri dish at a density of 1×108 cells/L for immunocytochemically staining.The cells were fixed with 40 g/L paraformaldehyde for 20 minutes.Immunocytochemical staining method was used to detect the expression of microtubule-associated protein-2 (MAP-2), neuron specific enolase(NSE), glial fibrillary acidic protein (GFAP) and choline acetyl transferase(ChAT). MAIN OUTCOME MEASURES: ① Morphological observation of rat' AECs at different culture time.② Expression of specific protein of neural cells in rat' cultured AECs. RESULTS: ① After cultured for 24 hours, the AECs were flat and presented fibroblast-like morphology. 3 to 5 days later, cell bodies were well stacked; AECs had a big and round nucleus and were connected with each other by flourishing dendrites. ② Immunocytochemical staining results after culture for 4 days showed that AECs expressed Nestin, ChAT, NSE, Musashi, MAP-2, GFAP. CONCLUSION: AECs are homologous to neural cells in morphology, and it may be a new cell source to treat nervous system disease.

背景:有证据表明羊膜上皮细胞能够表达神经系统细胞的几乎全部特异性抗原,且可以分泌多种神经营养因子及递质。若羊膜上皮细胞能够代替神经细胞,其神经营养作用必将为治疗神经推行性病变带来广阔前景。目的:检测神经组织细胞特异性抗原在大鼠羊膜上皮细胞中是否表达?设计:重复测量设计。单位:吉林大学第二临床医学院,吉林大学基础医学院组织与胚胎学教研室。材料:实验于2004-10/2005-10在吉林大学基础医学院组织与胚胎学教研室完成。选取清洁级孕12~14d的Wistar大鼠1只,将分离出的羊膜上皮细胞用于实验。小鼠抗大鼠神经元特异性抗原微管相关蛋白、星形胶质细胞特异性抗原胶质原纤维酸性蛋白、乙酰胆碱转移酶单克隆抗体、兔抗大鼠神经元特异性烯醇化酶和NT-3多克隆抗体(武汉博士德公司);大鼠抗大鼠Musashi抗体(日本庆应义塾大学岗野荣之教授惠赠)。方法:取孕12~14d的Wistar大鼠胎盘,剥离羊膜获得上皮细胞,在37℃、体积分数为0.05的CO2环境下,胰蛋白酶消化5min,加入DMEM/F12培养液,以5×109L-1的浓度接种于培养瓶中。培养3d后,细胞以1×108L-1的浓度接种于预先涂...

背景:有证据表明羊膜上皮细胞能够表达神经系统细胞的几乎全部特异性抗原,且可以分泌多种神经营养因子及递质。若羊膜上皮细胞能够代替神经细胞,其神经营养作用必将为治疗神经推行性病变带来广阔前景。目的:检测神经组织细胞特异性抗原在大鼠羊膜上皮细胞中是否表达?设计:重复测量设计。单位:吉林大学第二临床医学院,吉林大学基础医学院组织与胚胎学教研室。材料:实验于2004-10/2005-10在吉林大学基础医学院组织与胚胎学教研室完成。选取清洁级孕12~14d的Wistar大鼠1只,将分离出的羊膜上皮细胞用于实验。小鼠抗大鼠神经元特异性抗原微管相关蛋白、星形胶质细胞特异性抗原胶质原纤维酸性蛋白、乙酰胆碱转移酶单克隆抗体、兔抗大鼠神经元特异性烯醇化酶和NT-3多克隆抗体(武汉博士德公司);大鼠抗大鼠Musashi抗体(日本庆应义塾大学岗野荣之教授惠赠)。方法:取孕12~14d的Wistar大鼠胎盘,剥离羊膜获得上皮细胞,在37℃、体积分数为0.05的CO2环境下,胰蛋白酶消化5min,加入DMEM/F12培养液,以5×109L-1的浓度接种于培养瓶中。培养3d后,细胞以1×108L-1的浓度接种于预先涂有多聚赖氨酸的直径35mm平皿中,用质量浓度为40g/L的多聚甲醛固定20min。采用免疫细胞化学染色方法对神经元特异性抗原微管相关蛋白、神经元特异性烯醇化酶、星形胶质细胞特异性抗原胶质原纤维酸性蛋白、乙酰胆碱转移酶在羊膜上皮细胞中的表达情况进行检测。主要观察指标:①大鼠羊膜上皮细胞不同培养时间的形态观察。②神经组织细胞特异性抗原在大鼠羊膜上皮细胞中的表达情况。结果:①大鼠羊膜上皮细胞不同培养时间的形态观察:羊膜上皮细胞培养24h后,细胞扁平呈成纤维细胞样。3~5d后,胞体饱满,核大而圆,核仁清晰,突起发达,并互相连成网状。②神经组织细胞特异性抗原在大鼠羊膜上皮细胞中的表达情况:培养4d后免疫细胞化学染色结果可见,羊膜上皮细胞表达Nestin、神经元特异性烯醇化酶、乙酰胆碱转移酶以及Musashi、神经元特异性抗原微管相关蛋白、星形胶质细胞特异性抗原胶质原纤维酸性蛋白。结论:羊膜上皮细胞与神经组织细胞具有一定的同源性,可望作为治疗神经系统疾病新的细胞来源。

AIM: To observe the changes in development of embryonic metanephroi of mice at different age transplanted into adult allo-recipient of variant in the same race, and analyze the embryonic age selection and vascular genesis of embryonic metanephroi transplantation in mice. METHODS:The experiment was performed in the Department of Histology and Embryology, JinZhou Medical College from June 2003 to June 2004. A total of 24 Kunming mice were bred together. The time that pessary fell off was termed as 0 day embryonic...

AIM: To observe the changes in development of embryonic metanephroi of mice at different age transplanted into adult allo-recipient of variant in the same race, and analyze the embryonic age selection and vascular genesis of embryonic metanephroi transplantation in mice. METHODS:The experiment was performed in the Department of Histology and Embryology, JinZhou Medical College from June 2003 to June 2004. A total of 24 Kunming mice were bred together. The time that pessary fell off was termed as 0 day embryonic age, and the time that young mice born was termed as 0 day neonatal age. Mice with the embryonic age of 13 (E13), E14, E16 as well as mice with the neonatal age of 1 day were randomly divided into 4 groups E13, E14, E16 and N1 groups with 8 mice in each group. At the same time, 32 adult male mice of ordinary grade with the body mass of 20-25 g were taken as the recipients. Four embryonic metanephrois isolated from mice of E13 group were tissue-cultured, and the development of renal corpuscles were observed after 10 days. The metanephrois obtained from mice of E13, E14, E16 and N1 groups were transplanted into the adult allo-host'omentum, and light and electron microscope techniques were used to observe the developments of different parts in metanephroi (especially the renal corpuscles) after 10 days. for mouse after transplanted into an adult allo-host'omentum for 10 days. RESULTS: A total of 32 mice involved in all experiments entered the final analysis. ①No mature renal corpuscle was found in mice of the E13 group, and the isolated renal corpuscle could grow and differentiate after tissue culture and transplantation, in which there were mature renal corpuscles. ② Mature renal corpuscles were found in mice of the E14, E16 and N1 groups, and there were rejection, interstitial edema, infiltration in hemorrhage, homeocyte, shrank renal corpuscles after transplantation, even completely replaced by fibrous tissues. Besides, the extent of rejection was relate with the embryonic age. The order the embryonic age was, the stronger the rejection was. ③ The metanephroi structure before culture was the same as that of mice in the E13 group. There were renal corpuscles in each stage of development with complete structure of glomerulus under the electronic microscope. CONCLUSION: The rejection of transplantation before mature renal corpuscles emerge in the metanephroi of recipient is slighter with better development, which indicates that it's the best occasion for transplantation before glomerulus emerge.

目的:观察小鼠不同胚龄的后肾植入同种异体远交系成年宿主体内后的生长变化,分析小鼠胚胎后肾移植的胚龄选择、血管起源。方法:实验于2003-06/2004-06在锦州医学院组织与胚胎学教研室完成。实验选用普通级昆明系小白鼠24只,同笼饲养。以观察到阴道栓脱落的最早时间计为胚龄0d,以观察到仔鼠出生的最早时间计为生后0d。以随机数字表法取胚龄13,14,16d的胎鼠及生后1d的仔鼠,即胚龄13d组、胚龄14d组、胚龄16d组、生后1d组,每组8只。受体动物选择普通级体质量20g~25g的成年健康雄性小白鼠32只。对胚龄13d的小鼠胚胎离体后肾4只进行组织培养,观察10d后肾小体的发育情况。选择胚龄13,14,16d及生后1d的小鼠后肾植入同种远交系成年宿主大网膜内10d后,采用光镜及电镜技术观察后肾脏各部(特别是肾小体)的发育情况。结果:各组进行实验的后肾及宿主小白鼠32只均进入结果分析。①胚龄13d的小鼠肾脏中无成熟的肾小体,此胚龄的离体后肾经组织培养和移植后均可以生长和分化,产生成熟的肾小体。②胚龄14,16d及生后1d的小鼠肾脏中,已出现了成熟肾小体,后肾移植后则出现了排斥反应,间质水肿、出血区及...

目的:观察小鼠不同胚龄的后肾植入同种异体远交系成年宿主体内后的生长变化,分析小鼠胚胎后肾移植的胚龄选择、血管起源。方法:实验于2003-06/2004-06在锦州医学院组织与胚胎学教研室完成。实验选用普通级昆明系小白鼠24只,同笼饲养。以观察到阴道栓脱落的最早时间计为胚龄0d,以观察到仔鼠出生的最早时间计为生后0d。以随机数字表法取胚龄13,14,16d的胎鼠及生后1d的仔鼠,即胚龄13d组、胚龄14d组、胚龄16d组、生后1d组,每组8只。受体动物选择普通级体质量20g~25g的成年健康雄性小白鼠32只。对胚龄13d的小鼠胚胎离体后肾4只进行组织培养,观察10d后肾小体的发育情况。选择胚龄13,14,16d及生后1d的小鼠后肾植入同种远交系成年宿主大网膜内10d后,采用光镜及电镜技术观察后肾脏各部(特别是肾小体)的发育情况。结果:各组进行实验的后肾及宿主小白鼠32只均进入结果分析。①胚龄13d的小鼠肾脏中无成熟的肾小体,此胚龄的离体后肾经组织培养和移植后均可以生长和分化,产生成熟的肾小体。②胚龄14,16d及生后1d的小鼠肾脏中,已出现了成熟肾小体,后肾移植后则出现了排斥反应,间质水肿、出血区及淋巴细胞浸润,肾小体萎缩,甚至完全被纤维组织所取代;且排斥反应强弱与胚龄有关,胚龄越大,排斥反应越强。③培养前的后肾结构与移植前胚龄13d小鼠胚胎的后肾结构相同;培养10d后,可见出现了发育中各个阶段肾小体,电镜下可见血管球结构完整。结论:在供体后肾肾小体出现之前移植,其排斥反应轻微,生长发育良好,表明血管球形成之前是移植的最佳时间。

 
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