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rnasel基因
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  rnase l gene
     Construction of Plant Expression Vectors Carrying 2-5A and RNase L Gene and Transformation to Tobacco and Wheat
     2-5A和RNase L基因植物表达载体构建及对烟草和小麦的遗传转化
短句来源
     In this study, with the help of the intermidiate vector pJIT163,2-5A gene and Rnase L gene were cloned into the binary plant expression vector pBINplus to obtain the plant transformation/expression vector pBIN2-5A and pBINRL.
     本文利用2-5A基因和RNase L基因,通过中间载体pJIT163将2-5A基因和RNase L基因克隆到双元植物表达载体pBINplus上,分别构建了2-5A基因的植物表达载体pBIN2-5A和RNase L基因的植物表达载体pBINRL。
短句来源
     Co-transformed with plant expression vector p2-5A-RNase L carrying 2-5A gene and Rnase L gene and the expression vector pAHC carrying a herbicide-tolerance gene by means of the gene-gun method, 167 positive transformants ( TO) was acquired from about 5000 wheat embyos of cultivar"Yumai-18".
     利用2-5A基因和RNase L基因的单子叶植物表达载体p2-5A-RNase L以及除草剂抗性基因表达载体pAHC,采用基因枪共转化法,对小麦“豫麦18”进行遗传转化,共转化了大约10000个外植体(盾片),获得绿色转化株167株(T_0),经室内春化,温室栽种,获得了T_0种子;
短句来源
  “rnasel基因”译为未确定词的双语例句
     Wheat yellow mosaic virus (WYMV) coat protein gene, WYMV-72kD protein gene, WYMV-NIb protein gene, human 2-5A-RNase L gene were delivered into Wheat calli induced from immature embryos by others in our lab from 1999 to 2002. Seeds from these transgenic lines confirmed by PCR were sowed in fields heavily contaminated with WYMV to carry on field trail and molecular detection.
     1999年至2002年,本组先后将小麦黄色花叶病毒(WYMV,Wheat yellow mosaic virus)CP、72kD、NIb基因和来源于人的2-5A-RNase L基因转入小麦。 将上述PCR阳性的种子种于WYMV重病田进行田间试验和分子检测。
短句来源
     In the experiment of transgenic wheat with 2-5 A-RNase L, Southern blot analysis proved the existence of 2-5A in transgenic wheat genome (T2 generation). Ten transgenic line exhibited good resistance to WYMV in field trial.
     对于转2-5A-RNase L基因小麦,T2代的Southern blot结果证明了2-5A基因在转基因小麦基因组中的整合,而且十个株系在田间对该病呈现高度抗性。
短句来源
     New transgenic plants expressing various enzymes were conducted to initiate studies to evaluate broad spectrum resistance to viruses. The Ribonuclease L (RNase L) and 2',5'-synthetase (2-5A) genes were co-transformed into immature embryos of the inbred maize lines Z3 and Z31 by Agrobacterium tumefaciens LBA4404. In addition an RNase III gene and its mutation (Glu 117 Lys) were used to transform additional maize lines as above.
     以玉米(Zea mays)幼胚及幼胚诱导的愈伤组织为转化受体,利用农杆菌介导方法将2-5A体系的RNaseL基因和2-5A基因、RNaseⅢ基因(野生型和突变型)分别转入玉米自交系Z3和Z31,经G418筛选11周后,将抗性愈伤转移到高糖培养基上诱导分化成苗;
短句来源
     These vectors were used for Agrobacterium-mediated transformation of tobacco cultivar Yunyan-87 .The resultant transgenic plants transformated with 2-5A gene and RNaseL gene,respectively and inclusively were confirmed by PCR ,and their resistance to virus was measured by inoculation of TMV-B and ReMV respectively.
     利用冻融直接转化法把pBIN2-5A和pBINRL分别转入土壤农杆菌LBA4404中,在农杆菌介导下,转化烟草“云烟87”,分别获得了转2-5A基因和RNase L基因以及同时转此两种基因的再生植株。 对再生烟草植株进行了PCR检测,初步证明获得了转基因植株。
短句来源
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Overexpression of AK fbr gene in C.
     AKfbr基因在C.
短句来源
     New transgenic plants expressing various enzymes were conducted to initiate studies to evaluate broad spectrum resistance to viruses. The Ribonuclease L (RNase L) and 2',5'-synthetase (2-5A) genes were co-transformed into immature embryos of the inbred maize lines Z3 and Z31 by Agrobacterium tumefaciens LBA4404. In addition an RNase III gene and its mutation (Glu 117 Lys) were used to transform additional maize lines as above.
     以玉米(Zea mays)幼胚及幼胚诱导的愈伤组织为转化受体,利用农杆菌介导方法将2-5A体系的RNaseL基因和2-5A基因、RNaseⅢ基因(野生型和突变型)分别转入玉米自交系Z3和Z31,经G418筛选11周后,将抗性愈伤转移到高糖培养基上诱导分化成苗;
短句来源
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  rnase l gene
The transcriptional start site of the human RNase L gene was located in noncoding exon I by primer extension analysis.
      
Organization of the interferon-inducible 2',5'-oligoadenylate-dependent ribonuclease L (RNase L) gene of mouse
      
We have studied organization of RNase L gene in genomic DNA from the mouse liver by Southern blot analysis.
      
Mouse RNase L gene exists as a single copy (>amp;gt;16?kb DNA) gene.
      
Our results will help studying mouse RNase L gene promoter in further details.
      


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