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ppo基因
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  ppo gene
     The main experiment contents and results are as follows:1. Cloning of PPO gene: Complete PPO cDNA sequence of Helicoverpa armigera was obtained through RT-PCR and RACE, designated Ha-ppo (Genbank accession number: DQ114946) .
     1.棉铃虫PPO基因的克隆:利用RT-PCR和RACE相结合的方法,获得了棉铃虫PPO基因cDNA的全长序列,命名为Ha-ppo(GenBank序列号:DQ114946)。
短句来源
     We deduced that the PPO gene we got was Ha-ppo2.2. PPO transcription in three tissues of 5th larvae of Helicoverpa armigera: We investigated Ha-ppo2 transcription in three tissues of 5th larva of Helicoverpa armigera using RT-PCR.
     2.PPO基因的组织特异性表达分析:利用半定量RT-PCR方法研究了PPO基因在棉铃虫5龄幼虫体壁、血细胞、中肠这三种组织中的转录情况。
短句来源
     The sequence alignments revealed that at the position of intron I, the PPO gene in cultivars with low PPO activity has 191 bases more thanthat in cultivars with high PPO activity where PPO gene is expressed.
     因此推测在PPO 活性低的材料中,PPO 基因内含子I 中这段191-bp 的DNA片段插入可能影响了基因的表达,从而降低了PPO 活性;
短句来源
     The results showed that the PPO activity of most samples was repressed by anti-sense PPO gene. The PPO activity of "GD-9-qc-10" and "GD-9-qc-11" was only 2.8 and 4.1(0.01 OD·min-1), decreasing by 89.06 and 83.98 percent, respectively, compared with the control.
     结果表明:反义PPO基因对大多数转基因马铃薯试管苗PPO的活性产生了明显的抑制效果,其中GD-9-qc-10与GD-9-qc-11的PPO活性比对照降低89.06%和83.98%,且相应转基因品系的PPO同工酶也被明显抑制。
短句来源
     So,it was thought to be PPO gene fragment.
     据此推断克隆的片段为PPO基因片段。
短句来源
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  “ppo基因”译为未确定词的双语例句
     4. PPO-1 and PPO-2 genes were located in 2A and 2D chromosomes through nullisomic-tetrasomic lines of Chinese Spring cultivars.
     4 利用中国春的缺体-四体材料,将两个PPO基因(PPO-1,PPO-2)分别定位在2A和2D上。
短句来源
     Nine kinds of PPO genes have been cloned from Anopheles gambiae, 1 from Anopheles stephensi and 1 from Anopheles culicifacies to date.
     目前,从冈比亚按蚊(Anopheles gambiae)已克隆到9种PPO基因,从斯氏按蚊(Anopheles stephensi)、库态按蚊(Anopheles culicifacies)均只克隆到一种PPO基因
短句来源
     Optimization of Potato Anti-PPO Gene Transformation System and Analysis of PPO Activity and PPO Isozyme of Transgenic Plants
     马铃薯反义PPO基因遗传转化体系的优化及转基因植株PPO活性、同工酶的分析
短句来源
     Preparation of monoclonal antibody against PPO and studying the expression in malignant melanoma
     PPO基因单克隆抗体的制备以及在恶性黑色素瘤中表达的研究
短句来源
     In addition, a intron with ‘GC-AG’characteristics which was not reported previously in plant genes has also been found.
     另外还发现了小麦PPO 基因内含子II为‘GC-AG’型内含子,不同于内含子的‘GT-AG’典型特征,这在高等植物中尚属首次报道。
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     So,it was thought to be PPO gene fragment.
     据此推断克隆的片段为PPO基因片段。
短句来源
     The NiCoT gene of S. aureus had been successfully expressed in E.
     外源基因在E.
短句来源
     Ovarian cancer cell line can be suppressed by RNA interference
     RNA干扰PPO基因抑制卵巢癌细胞生长
短句来源
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  polyphenol oxidase (ppo)
Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively.
      
Activity indicating the presence of two oxidoreductases, polyphenol oxidase (PPO) and peroxidase (Px), was found.
      
Molecular characterization of wheat polyphenol oxidase (PPO)
      
It is well-established that the enzyme polyphenol oxidase (PPO) is involved in undesirable browning of noodles, chapattis, middle east flat breads and steamed breads.
      
Polyphenol oxidase (PPO) in wheat and wild relatives: molecular evidence for a multigene family
      
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  ppo gene
However, there is no report on the identification and characterization of a wheat PPO gene.
      
Sequence analysis identified the conserved amino acids of PPO genes indicating that the PCR product was part of the wheat PPO gene.
      
Screening genomic and cDNA libraries using 444- and 760-bp DNA fragments as probes failed to identify a PPO gene based on conserved sequence, even though there were very strong hybridization signals for some isolates.
      
Rapid amplification of cDNA ends (RACE) technique was used as an alternative to obtain the remaining DNA sequences in 5' and 3' directions based on the 444-bp partial wheat PPO gene sequence.
      
Alignment of deduced amino-acid sequences revealed similarity to the other PPO gene sequences, especially in the conserved copper binding regions.
      
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Polyphenol oxidase (PPO) is a metalcontained plastidic enzyme. It occurs ubiquitously in plants, and can catalyze phenols to quinones. PPOs are nuclear encoded, synthesized in cytoplasm, transferred into plastids in some forms, and then they become of enzyme activity. Substrates of PPO exit in vacuole. The compartmentalization of PPO and its substrates makes it difficult to ensure the functions of PPO. PPO genes from tomato, potato,apple, grape and other plants are successfully cloned.PPO gene appears to be...

Polyphenol oxidase (PPO) is a metalcontained plastidic enzyme. It occurs ubiquitously in plants, and can catalyze phenols to quinones. PPOs are nuclear encoded, synthesized in cytoplasm, transferred into plastids in some forms, and then they become of enzyme activity. Substrates of PPO exit in vacuole. The compartmentalization of PPO and its substrates makes it difficult to ensure the functions of PPO. PPO genes from tomato, potato,apple, grape and other plants are successfully cloned.PPO gene appears to be a gene family.

多酚氧化酶(PolyphenolOxidase简称PPO)是一类广泛分布于植物体内能催化多酚类氧化成醌类的质体金属酶.PPO由核基因编码,在细胞质中合成,通过一定的方式转运至质体内而成为具酶活性的形式.PPO的底物(酚类物质)存在于液泡.这种酶与底物的区域分布,使得PPO在完整细胞内的生理功能难以确定.PPO基因已分别从番茄、马铃薯、苹果、葡萄等植物中克隆成功.PPO基因属于一个基因家族.

Objective To clone the partial cDNA sequence of prophenoloxidase gene of Anopheles dirus(AdPPO). Methods Degenerative primers were designed according to the conserved sequence blocks within the prophenoloxidase gene of insects, RNA sequence of adults of An. dirus is reverse transcripted and amplified to get the prophenoloxidase cDNA which is cloned into T vector and sequenced. The partial cDNA sequence of AdPPO was analysed and compared with other prophenoloxidase gene of insects. Results The partial cDNA...

Objective To clone the partial cDNA sequence of prophenoloxidase gene of Anopheles dirus(AdPPO). Methods Degenerative primers were designed according to the conserved sequence blocks within the prophenoloxidase gene of insects, RNA sequence of adults of An. dirus is reverse transcripted and amplified to get the prophenoloxidase cDNA which is cloned into T vector and sequenced. The partial cDNA sequence of AdPPO was analysed and compared with other prophenoloxidase gene of insects. Results The partial cDNA sequence of AdPPO was 598 bp and its deduced amino acid sequence is 199aa. The cDNA sequence homology and amino acid sequence homology is 84.23% and 88.89% respectively compared with the PPO5 gene of Anopheles gambiae. Conclusion The AdPPO is successfully cloned and it has very high sequence similarity with the PPO gene of An. gambiae.

目的 克隆大劣按蚊 (Anophelesdirus)前酚氧化酶 (Prophenoloxidase,PPO)基因部分序列。 方法 根据已报道的多种昆虫 PPO的保守氨基酸序列设计简并引物 ,以大劣按蚊总 RNA为模板进行 RT- PCR扩增 ,目的片段经 TA克隆后测定其核酸序列 ,然后进行序列查询与比对。 结果 大劣按蚊 PPO基因 (Ad PPO)部分序列长 5 98bp,编码 199个氨基酸 ;Ad PPO与冈比亚按蚊 PPO5基因的核酸和氨基酸序列同源性分别达 84 .2 3%和 88.89%。 结论 成功克隆出Ad PPO部分序列 ,其与冈比亚按蚊 PPO基因序列具有很高同源性。

Objective To clone the partial cDNA sequences of new members of prophenoloxidase (PPO) gene family from Anopheles dirus and to localize their mRNA in the mosquitoes. Methods Degenerative primers were designed according to the conserved amino acid sequence within PPOs of insects. RNA sequence of adults of Anopheles dirus was reversely transcripted and amplified. The obtained PPO cDNA was cloned into T vector. The new AdPPOs were obtained by screening of the positive clones using PCR and sequencing....

Objective To clone the partial cDNA sequences of new members of prophenoloxidase (PPO) gene family from Anopheles dirus and to localize their mRNA in the mosquitoes. Methods Degenerative primers were designed according to the conserved amino acid sequence within PPOs of insects. RNA sequence of adults of Anopheles dirus was reversely transcripted and amplified. The obtained PPO cDNA was cloned into T vector. The new AdPPOs were obtained by screening of the positive clones using PCR and sequencing. The localization of their mRNA in the hemolymph, midguts, and salivary glands was analyzed by RT PCR. Results The partial cDNA sequences of AdPPO2 and AdPPO3 were 597 bp and their deduced amino acid sequences were 199aa. Their mRNA could be found in the hemolymph, midguts, and salivary glands. Conclusion The partial cDNA sequences of the two AdPPO members from Anopheles dirus have been successfully cloned, providing the foundation for cloning their full length sequences.

目的 克隆大劣按蚊PPO基因家族新成员的cDNA部分序列 ,并对所克隆基因进行蚊体组织定位研究。方法 根据已报道的多种昆虫PPO的保守氨基酸序列设计简并引物 ,以大劣按蚊总RNA为模板进行RT PCR扩增、目的片段克隆入T载体 ,然后 ,用PCR方法筛选新的PPO克隆并测序 ;以RT PCR方法分析AdPPO2和AdPPO3在蚊血淋巴、蚊胃和唾液腺的分布情况。结果 AdPPO2和AdPPO3基因cDNA部分序列长均为 5 97bp ,编码 199个氨基酸 ;它们在血淋巴、蚊胃和唾液腺均有分布。结论 从大劣按蚊成功克隆获得 2种新的PPO基因家族成员的cDNA部分序列 ,为进一步克隆其全序列奠定了基础

 
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