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骨髓mscs
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     Results: The MSCs were positive for CD29、CD44 and negative for CD15、CD33、CD34、HLA-DR.
     结果大鼠骨髓MSCs细胞表面抗原CD29、CD44阳性,CD15、CD33、CD34、HLADR阴性;
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     the transplantation of the human MSCs and CL-1 cells can ameliorate the recovery of the 2-AAF+CCL4+CP rats;
     肝内移植人骨髓MSCs和CL一1细胞均有助于2一AAF+CCL4+CP大鼠肝损伤后肝功能的恢复;
短句来源
     (3) The level of HGFs(TPO,SCF,FLT-3L,IL-6) secreted by different sources of MSCs was checked by ELISA method.
     (3)ELISA法检测脐血和骨髓MSCs培养上清中SCF、TPO、FLT-3L和IL-6的分泌水平。
短句来源
     The cultured adherent cells typically expressed CD29,CD44,CD166 and CD105,while CD34,CD45,HLA-DR of them were negative.
     流式细胞术检测结果显示,此次培养的骨髓来源细胞高表达CD29、CD44、CD166、CD105,不表达CD34、CD45、HLA-DR,符合骨髓MSCs细胞的特性。
短句来源
     Results The expressions of CD29, CD44, CD73 (SH-3, 4), CD105 (SH-2), CD166, HLA-ABC in MSCs from fetal liver and fetal bone marrow were positive, CD35, CD45, HLA-DR were negative. MSCs from fetal liver and bone marrow were successfully cultivated and differentiated into osteoblast and adipose in vitro. As the quantity of MSCs increased, their inhibitory effects on transformation of lymphocytes in peripheral blood enhanced.
     结果:胎儿肝脏及胎儿骨髓MSCs CD29、CD44、CD73(SH-3,4)、 CD105(SH-2)、CD166、HLA-ABC阳性,CD35、CD45、HLA-DR阴性,均能成脂肪及成骨诱导分化,随 MSCs数量增加,其对外周血T淋巴细胞转化的抑制作用增强。
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     identifying MSCs differetiating into cardiomyocytes;
     鉴定骨髓MSCs的分化;
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     Conclusion The cultured cells are identified the bone marrow MSCs.
     结论培养的细胞为骨髓MSCs;
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     Bone Marrow Necrosis
     骨髓坏死
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     MSCs grew rapidly, developed into visible symmetric colonies at about 3 days and reached confluence at 7 to 10 days.
     MSCs经10!
短句来源
     Hematopoietic Stem Cell Niche in the Bone Marrow
     骨髓造血干细胞龛
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  marrow stroma cell
We have analysed the expression, synthesis, cell distribution and formation of functional gap junctions in the murine bone-marrow stroma cell line S-17, and between stromal cells and blood cell progenitors.
      
The transversal differentiation of bone marrow stroma cell (BMSCs) into neural stem cells (NSCs) has attracted much attention in recent years because of their therapeutic potential.
      


Bone marrow mesenchymal stem cells (MSCs) are multipotential progenitors of connective tissues and bone marrow stroma as well, which implies the modulatory function of MSCs in hematopoiesis. To clarify the contributions of MSCs to hematopoiesis, the methods for isolation and expansion of MSCs were established and long term bone marrow cultures were performed using irradiated MSCs as the feeder layer. The results here showed that CD34 + cells from cord blood formed hematopoietic foci adherent to the monolayer....

Bone marrow mesenchymal stem cells (MSCs) are multipotential progenitors of connective tissues and bone marrow stroma as well, which implies the modulatory function of MSCs in hematopoiesis. To clarify the contributions of MSCs to hematopoiesis, the methods for isolation and expansion of MSCs were established and long term bone marrow cultures were performed using irradiated MSCs as the feeder layer. The results here showed that CD34 + cells from cord blood formed hematopoietic foci adherent to the monolayer. Furthermore, colony forming cells remained in the coculture of 5 weeks, indicating the maintenance of long term culture initiating cells (LTC IC). Flow cytometry analysis showed that about 1% of the hematopoietic cells in the culture were positive for CD34 and around 15% were CD41a positive. It is clear that MSCs maintain LTC IC in vitro and promote differentiation of hematopoietic progenitors especially into megakaryocytic lineage. The preliminary results here demonstrate that MSCs residing in the bone marrow might be a crucial cellular component in the hematopoietic microenvironment.

体内的稳态造血依赖于复杂而完整的骨髓造血微环境系统 ,其中的细胞成分是该系统的关键。存在于骨髓中的间充质干细胞 (MSCs)是成纤维细胞、内皮细胞、成骨细胞、脂肪细胞等多种骨髓基质细胞的前体细胞 ,在造血调控中可能具有一定的作用。本研究首先建立了成人骨髓MSCs的分离及体外培养的方法 ,并应用长期骨髓细胞培养体系 ,观察了MSCs滋养层体外维持长期培养启动细胞 (LTC IC)的能力及其促进造血细胞分化的功能。结果显示 :①脐带血来源的CD34+细胞粘附于滋养层上形成造血灶 ,表明MSCs可形成与骨髓基质细胞相似的体外造血微环境 ;②共培养 5周后造血细胞仍具有体外集落形成能力 ,说明MSCs具有维系LTC IC的能力 ;③流式细胞术分析显示 ,体外培养 5周后约 1%悬浮细胞表达CD34,15 %细胞CD4 1a阳性 ,提示MSCs促进造血细胞向巨核系细胞分化。研究证明MSCs可能是造血微环境中的重要细胞成分

Objective To study the feasibility of constructing tissue engineered cartilage by differentiated rabbit bone marrow mesenchymal stem cells(MSC) cultured in vitro and in vivo . Methods The MSC were isolated from the nucleated cells fraction of autologous bone marrow by density gradient centrifuge, and then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor β 1(TGF β 1) and ascorbic acid in vitro . After 3 weeks, some cells turned to round shape and secreted...

Objective To study the feasibility of constructing tissue engineered cartilage by differentiated rabbit bone marrow mesenchymal stem cells(MSC) cultured in vitro and in vivo . Methods The MSC were isolated from the nucleated cells fraction of autologous bone marrow by density gradient centrifuge, and then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor β 1(TGF β 1) and ascorbic acid in vitro . After 3 weeks, some cells turned to round shape and secreted metachromatic matrix. The cartilaginoid grafts composed of chondrogenic MSC. Bovine type Ⅰ collagen and human fibrin were cultured within the chondrogenic medium for 2 weeks in vitro or transplanted subcutaneously adjacent to the knee joint for 3 weeks in vivo . Results The most cells in the grafts were degenerated and disappeared after cultured in vitro . But the residual cells were survival and secreted metachromatic staining proteoglycan with toluidine blue, which was characteristic cartilage matrix. The grafts developed into matured cartilage tissue assessed by histological examination after 3 weeks of transplantation in vivo . Conclusion MSC can be used as functional cells to constructing tissue engineered cartilage.

目的 采用组织工程方法 ,以培养后的兔骨髓间质干细胞 (MSC)制成人工软骨培养物 ,经体内外培养后发育出成活的软骨组织。方法 抽取兔骨髓液经密度梯度离心得到单个核细胞 ,再经体外分离、培养获得兔骨髓 MSC。向 MSC培养液内加入地塞米松、转化生长因子 -β1 (TGF-β1 )和维生素 C进行软骨起源诱导培养 3周 ,部分细胞开始转变为圆形并分泌基质。将诱导后的细胞与牛 型胶原及人纤维蛋白按一定的比例混合 ,制成软骨样的培养物并分别做体内外培养。结果 体外培养 2周后 ,培养物内大部分细胞已萎缩消失。但剩余的少量细胞成活 ,形成类似的软骨陷窝并分泌甲苯胺蓝异染的软骨基质。体内移植培养 3周后 ,培养物已发育成颗粒状成熟的软骨组织。结论 骨髓间质干细胞可用于组织工程软骨组织的构建 ,是一种非常有前途的人工软骨组织构建中的功能细胞。

Objective To observe the main biological characteristics of marrow-derived mesenchymal stem cells (MSCs) during the passage cultivation under the condition for chondrocyte culture. Methods The marrow-derived MSCs were isolated from young rabbits and cultivated in the condition for culturing chondrocytes. The changes of morphology, growth and proliferation, synthesis of collagen typeⅠ and Ⅱ and aggrecan, and activity of alkaline phosphatase (ALP) were observed during the passage cultivation of MSCs. Results...

Objective To observe the main biological characteristics of marrow-derived mesenchymal stem cells (MSCs) during the passage cultivation under the condition for chondrocyte culture. Methods The marrow-derived MSCs were isolated from young rabbits and cultivated in the condition for culturing chondrocytes. The changes of morphology, growth and proliferation, synthesis of collagen typeⅠ and Ⅱ and aggrecan, and activity of alkaline phosphatase (ALP) were observed during the passage cultivation of MSCs. Results 1) Primary MSCs proliferated in visible symmetric colonies with long-spindle shape. The morphological characteristics of marrow-derived MSCs had no change during passaging, and its homogenous rose with passaging. 2) Every passage cells showed positive staining of collagen typeⅠ, and negative of collagen typeⅡ. 3) The cells had negative staining of ALP, and weak heterochromatic to toluidine blue. The content of sulfate glycosaminoglycans (GAG) was very little in extracellular matrix. Conclusion The biological characteristics of MSCs in the basic condition for culturing chondrocytes are the same with in the regular low-glucose condition for culturing MSCs.

目的 观察骨髓间充质干细胞 (Mesenchymalstemcells ,MSCs)在软骨细胞培养条件下传代培养的主要生物学特性。方法 梯度离心分离幼兔骨髓有核细胞 ,培养分离MSCs并进行传代培养 ,观测在体外软骨细胞培养条件下MSCs形态、生长特点及Ⅰ型和Ⅱ型胶原、蛋白多糖聚集体合成分泌以及碱性磷酸酶活性等方面的变化。结果 ①MSCs原代细胞呈可见的均匀分布的簇状生长 ,呈长梭形 ,在传代培养中形态特性无明显变化、均质性明显提高 ;②各代Ⅰ型胶原免疫组化均为阳性 ,Ⅱ型胶原免疫组化均为阴性。③在各代培养中碱性磷酸酶染色均为阴性 ,对甲苯胺蓝存在极弱的异染性反应 ;④细胞外基质中硫酸糖胺多糖含量亦很低。结论 传代培养中骨髓MSCs保持了常规低糖培养条件下的基本生物学特点 ,说明常用的软骨细胞基础培养条件同样适合骨髓MSCs的培养 .

 
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