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   western免疫印迹 的翻译结果: 查询用时:0.499秒
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western免疫印迹
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  “western免疫印迹”译为未确定词的双语例句
     Western blotting analysis was used to detect the protein expression of cyclin B1 and phosphorylation change of P34~(cdc2).
     Western免疫印迹分析cyclin B1,P34cdc2和磷酸化P34cdc2(phospho-cdc2,酪氨酸15)蛋白表达;
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     Western blotting analysis showed that treatment of CaSki cells with ISL remarkably decreased the expression of cyclin B1 at 48h and enhanced the level of P34~(cdc2) phosphorylation from 16 h;
     Western免疫印迹分析显示,ISL作用24 h cyclin B1蛋白表达开始不同程度下降,48 h下降明显,P34cdc2变化较小,ISL作用16 h后磷酸化P34cdc2(酪氨酸15)蛋白水平明显改变;
短句来源
     Expression levels of PKCε protein were determined by Western immunoblotting.
     Western免疫印迹测定PKCε蛋白质表达水平。
短句来源
     Immunohistochemistry and quantitative analysis of Western blot were applied to detect the protein expression of ET,1, cNOS, iNOS.
     应用免疫组化和半定量Western免疫印迹方法检测ET1、cNOS、iNOS在食管下端的表达。
短句来源
     Expressive levels of PKCε protein were determined by Western immunoblotting.
     Western免疫印迹测定PKCε蛋白质表达水平。
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  相似匹配句对
     1. Western - blotting:
     1.Western免疫印迹杂交结果:
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     The VEGF protein in the tissue from heart was detected by using Western blot.
     采用 Western免疫印迹方法检测 VEGF蛋白质。
短句来源
     Western blot;
     Western blot;
短句来源
     The amount of protein was highly correlated with the antigenic activity when analysis the sample by
     3.免疫印迹分析
短句来源
     MODIFICATIONS OF THE PROTEIN IMMUNOBLOTTING TECHNIQUES
     免疫印迹技术的一些改进
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  western blots
Furthermore, Western blots revealed that the Bcl-2 expression was relatively high in the sensitive primary leukemia cells, and especially the cleavage of 26 ku Bcl-2 into a 22 ku fragment occurred during the induction of apoptosis.
      
Immuno-western blots, immunofluorescence and immuno-gold labeling methods were used to study the existence and distribution of a homologue of the membrane skeleton spectrin in the pollen and pollen tube ofLilium davidii Duch.
      
Patients were monitored serologically by ELISA test and Western Blots within 2-5 years after initiation of chemotherapy/surgery.
      
The apparent molecular mass of the Hyper-CNTF protein was estimated from western blots to be 75 kDa.
      
Western blots showed an increase expression of ETA receptor protein in injured vessels compared to non-injured arteries.
      
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DNA fragments of C831(530bp)and C33c (860bp)encoding the putative nucleocapsid(C)CL and the nonstructural region 3(NS3) protein C33c of the HCV, have been obtained fromthe sera of Chinese carriers with HCV infection by reverse transcription (RT)and polymerasechain reaction(PCR) techniques. The 5' terminal of C831 cDNA fragment was linked upup with the 3'terminal of NS3 cDNA fragment by a oligonucleotide linkerSer-Pro-Gly-Ser to form a chimeric gene C33c-C831(1400bp)The chimeric geneC33c-C831 was recombined with...

DNA fragments of C831(530bp)and C33c (860bp)encoding the putative nucleocapsid(C)CL and the nonstructural region 3(NS3) protein C33c of the HCV, have been obtained fromthe sera of Chinese carriers with HCV infection by reverse transcription (RT)and polymerasechain reaction(PCR) techniques. The 5' terminal of C831 cDNA fragment was linked upup with the 3'terminal of NS3 cDNA fragment by a oligonucleotide linkerSer-Pro-Gly-Ser to form a chimeric gene C33c-C831(1400bp)The chimeric geneC33c-C831 was recombined with prokaryotic expression vector pBV220,and was expressed inE. coli in the form of a native chimeric polyprotein C33c-CL.The expression product wasscreened and detected by enzyme-linked immune solid assay and western blotting withanti-C33c serum and anti-CL serum,respectively.A chimeric C33c-CL polyprotein with amolecular weight of 53kD was acounted for 9% of the total cellular soluble proteins.The expression product was extracted from the bacterial lysate by lysozyme, Triton X-100and urea treatment and purified by ion exchange chromatography. The purified C33c-CLchimeric polyprotein was used to develop a capture assay for reactive anti-HCV antibodies.This anti-C33c-CL assay detected all previously identified HCV-seropositive cases andprovides a substantially more sensitive diagnosis(99%)than any of anti-C33c(93%)anti-C22(83%)or anti-5-1-1(50%) for both acute and chronic HCV infections. TheC33c-CL chimeric polyprotein keeps the antigenicity of both C33c and CL. Its specificityand sensitivity were all in keep with the requirements of the national standard for qualitycontrol of the HCV diagnostic kit.The C33c-CL chimeric polyprotein may have animportant role in anti-HCV assay.

通过逆转录(RT)-聚合酶链式反应(PCR),从中国人丙型肝炎病毒(HCV)携带者的血清中扩增并克隆到2段cDNA片段,即HCV基因组C区抗原基因C831cDNA片断(约530bp)和NS3区抗原基因C33ccDNA片段(约860bp)。C33ccDNA片段同C831cDNA片段经连接   肽Ser-Pro-Gly-Ser连接成为基因嵌合体C33c-C831(约1400bp)。C33c-C831基因嵌合体同温控型原核表达载体pBV220重组,构建成表达质粒pBV/C33c-C831,并在大肠杆菌细胞中获得了重组嵌合抗原C33c-CL的表达。通过酶切分析和Western免疫印迹法,对约占菌体可溶性蛋白9%的表达产物做了鉴定。采用TritonX-100和盐析处理,获得粗提表达产物。粗提的表达产物经尿素裂解和离子交换层析纯化,得到可用于检测抗HCV核壳蛋白和抗NS3区抗体的重组嵌合抗原C33c-CL。对C33c-CL做抗原性分析发现,它同时具有完整的C33c抗原和C22抗原的免疫反应活性,完全能替代单纯的C33c和C22抗原。该嵌合抗原在血清学诊断中有重要的应用价值,可望成为新一代HCVEIA诊断试剂的...

通过逆转录(RT)-聚合酶链式反应(PCR),从中国人丙型肝炎病毒(HCV)携带者的血清中扩增并克隆到2段cDNA片段,即HCV基因组C区抗原基因C831cDNA片断(约530bp)和NS3区抗原基因C33ccDNA片段(约860bp)。C33ccDNA片段同C831cDNA片段经连接   肽Ser-Pro-Gly-Ser连接成为基因嵌合体C33c-C831(约1400bp)。C33c-C831基因嵌合体同温控型原核表达载体pBV220重组,构建成表达质粒pBV/C33c-C831,并在大肠杆菌细胞中获得了重组嵌合抗原C33c-CL的表达。通过酶切分析和Western免疫印迹法,对约占菌体可溶性蛋白9%的表达产物做了鉴定。采用TritonX-100和盐析处理,获得粗提表达产物。粗提的表达产物经尿素裂解和离子交换层析纯化,得到可用于检测抗HCV核壳蛋白和抗NS3区抗体的重组嵌合抗原C33c-CL。对C33c-CL做抗原性分析发现,它同时具有完整的C33c抗原和C22抗原的免疫反应活性,完全能替代单纯的C33c和C22抗原。该嵌合抗原在血清学诊断中有重要的应用价值,可望成为新一代HCVEIA诊断试剂的优选抗原。

Sixty-six patients with retinal diseases and seventeen healthy aged persons were tested for serum autoantibodies against normal human retinal protein by western immunoblot analysis. Forty-threc out of 66 patients showed positive responses, with a rate of 65 per cent. The positive rate of antiretinal antibodies in the patients with age-related macular degeneration was significantly higher than that in the healthy aged (P<0.01). About half or more patients with retinitis pigmentosa and idiopathic preretinal membanes...

Sixty-six patients with retinal diseases and seventeen healthy aged persons were tested for serum autoantibodies against normal human retinal protein by western immunoblot analysis. Forty-threc out of 66 patients showed positive responses, with a rate of 65 per cent. The positive rate of antiretinal antibodies in the patients with age-related macular degeneration was significantly higher than that in the healthy aged (P<0.01). About half or more patients with retinitis pigmentosa and idiopathic preretinal membanes presented positive antiretinal antibodies in their sera but seldom in those with central serous chorioretinopathy and acute retinal necrosis. The nature of retinal antigens, with which the antibodies reacted, along with effects on the various retinal diseascs was also discussed.

应用Western免疫印迹技术测定66例视网膜病病人抗视网膜抗体,43例出现阳性反应(65%).老年黄斑变性病人血清抗视网膜抗体阳性反应极为显著,明显高于健康老人(P<0.02);视网膜色素变性、特发性视网膜前膜患者血清也有一定的抗视网膜抗体阳性率;而中心性浆液性视网膜脉络膜病变及急性视网膜坏死抗视网膜抗体反应不明显。本文还讨论了这些抗体所作用的抗原的性质及其在这些疾病中的作用。

The synthetic DNA encoding for N-terminal dominant epitopes of core region of HCV polyprotein was synthesized with a semi-chemical and semi-enzymatic method and identified through DNA sequencing. The DNA fragment was inserted into the prokaryotic expression vector pGEX-ZT to express fusion protein GST-C74. Afer purified by affinity purification,the product was analysed with Western blot and indirect ELISA, the results showed that GST-C74 has the similar antigenity as core antigen as expected,thus it could be...

The synthetic DNA encoding for N-terminal dominant epitopes of core region of HCV polyprotein was synthesized with a semi-chemical and semi-enzymatic method and identified through DNA sequencing. The DNA fragment was inserted into the prokaryotic expression vector pGEX-ZT to express fusion protein GST-C74. Afer purified by affinity purification,the product was analysed with Western blot and indirect ELISA, the results showed that GST-C74 has the similar antigenity as core antigen as expected,thus it could be used in anti-HCV immunoassay. On the other hand,synthetic DNA fragment coding HCV dominant epitopes will be beneficial to the research of chimeric antigens.

依据丙型肝炎病毒(HCV)多蛋白核心区N端氨基酸序列和密码子简并性,人为设计并采用半化学半酶促的方法合成了一个DNA片段。经核酸杂交检测以及DNA序列分析证实,该片段的核苷酸序列与设计完全一致。将合成的DNA片段插入融合表达载体pGEX-2T中,表达产物经亲和层析纯化后进行Western免疫印迹实验和间接ELISA分析,结果表明,融合蛋白具有HCV核心区抗原的免疫反应性,可望用于HCV抗体的检测.此外,编码HCV优势抗原表位的化学合成基因有可能为HCV嵌合抗原的研究提供一条捷径。

 
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