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人参愈伤组织细胞
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  ginseng callus cells
     The Study of Human Interferon-alpha 2b Expressed by Ginseng Callus Cells
     人参愈伤组织细胞表达人干扰素α 2b的研究
短句来源
     The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-α2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation.
     用冻融法将pBIFN导入根瘤农杆菌LBA4404,经卡那霉素和利福霉素筛选后利用农杆菌介导的转化法将hIFN-α2基因导入人参愈伤组织细胞
短句来源
     Results:Stable integration of the hIFN-α2b gene in the Ginseng callus cells′ genome was confirmed by PCR analysis.
     结果:经PCR检测表明hIFN-α2b基因已成功整合到人参愈伤组织细胞基因组中。
短句来源
  ginseng cell
     Study on Hepatitis B Surface Antigen in Transgenic Ginseng Cell Line
     人参愈伤组织细胞系表达乙肝病毒表面抗原的研究
短句来源
  “人参愈伤组织细胞”译为未确定词的双语例句
     Western blot implied that the given protein was hIFN-α2b.
     Western blot分析表明转基因人参愈伤组织细胞能有效表达hIFN-α2b蛋白。
短句来源
     ULTRASTRUCTURAL STUDIES ON THE CALLUS CELLS OF PANAX GINSENG C.A.MEY
     人参愈伤组织细胞亚显微结构的研究
短句来源
     Construction of plant expression vector containing human interferon gene and expression in Ginseng calli
     人干扰素基因植物表达载体的构建及其在人参愈伤组织细胞中的表达
短句来源
     Ginseng cells were co-cultivated for 48-72 h with Agrobacterium tumefaciens carrying pBIBSa.
     本研究将表达载体质粒pBIBSa转化到农杆菌LBA4404中,经预培养后,与人参愈伤组织细胞在67V-AS培养基上进行共培养转化;
短句来源
     Thus, the eukaryoticexpression vector pBIFN including huIFN-α2b gene was constructed. Andthen the pBIFN was transformed into Agrobacterium tumefaciens strainLBA4404. After that, huIFN-α2b gene was introduced into Ginseng calluscells via Agrobacterium-mediated transformation.
     我们应用PCR 和:DNA 重组技术构建出含有人干扰素a 2b 基因的植物细胞表达载体pBIFN,然后转化根癌农杆菌LBA4404,利用农杆菌介导的转化法将人干扰素a 2b 基因导入人参愈伤组织细胞并整合到其基因组中。
短句来源
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  相似匹配句对
     ULTRASTRUCTURAL STUDIES ON THE CALLUS CELLS OF PANAX GINSENG C.A.MEY
     人参愈伤组织细胞亚显微结构的研究
短句来源
     SUSPENSION CULTURE OF STEM CALLUS CELLS OF PANAX GINSENG
     人参愈伤组织细胞悬浮培养
短句来源
     STUDIES ON THE INDUCTION OF CALLUS OF PANAX GINSENG
     人参愈伤组织的诱导
短句来源
     The Study of Human Interferon-alpha 2b Expressed by Ginseng Callus Cells
     人参愈伤组织细胞表达人干扰素α 2b的研究
短句来源
     Study on Hepatitis B Surface Antigen in Transgenic Ginseng Cell Line
     人参愈伤组织细胞系表达乙肝病毒表面抗原的研究
短句来源
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  ginseng callus cells
Expression of the Agrobacterium rhizogenes rolC gene in Panax ginseng callus cells results in formation of tumors that are capable to form roots.
      
  ginseng cell
Assessment of various carbon sources and nutrient feeding strategies for Panax ginseng cell culture
      
Sucrose was shown to be the superior carbon source to the monosaccharides for ginseng cell growth and the optimal concentration was between 30 and 50 g/L.
      
US treatment stimulated the synthesis of useful secondary metabolites, saponins of ginseng cells, without causing any net loss of the biomass yield of ginseng cell cultures.
      
The impacts of cryoprotectants (CP) and cell status during the growth cycle on Panax ginseng cell viability during cryopreservation were investigated.
      
This study was initiated to investigate the impacts of elicitor concentration and elicitor-adding time on the saponin synthesis and the cell growth of Panax ginseng cell suspensions.
      
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When ginseng (Panax ginseng C. A. Meyer ) cells were subjectedto horizontal rotation on a clinostat, theirgrowth and ginseng saponin content differed from those cultured in normal Bothitational environments (control ). Bothfresh and dry weights of ginseng cells rotating on clinostat were higher than thoseof the control, and the difference in dryweight was particularly obvious (Fig. 1 ).After 3 weeks of cultivation, saponincontent in ginseng was 10 % higher under the horizontal rotation treatment onthe clinostat...

When ginseng (Panax ginseng C. A. Meyer ) cells were subjectedto horizontal rotation on a clinostat, theirgrowth and ginseng saponin content differed from those cultured in normal Bothitational environments (control ). Bothfresh and dry weights of ginseng cells rotating on clinostat were higher than thoseof the control, and the difference in dryweight was particularly obvious (Fig. 1 ).After 3 weeks of cultivation, saponincontent in ginseng was 10 % higher under the horizontal rotation treatment onthe clinostat than that of the control (Fig. 3). When ginseng cells were cultured on Ca2+ -deprived medium and clinostatted for 3 weeks, their ginsengsaponin content was almost twice of thatof the control (Fig. 4). Besides, in ourexperiments, the higher the Ca2+ concentration in medium, the lower the ginseng saponin content in the ginseng cellscultured (Fig. 2).

与在正常重力条件培养下的对照相比,经回转器水平回转处理的人参细胞鲜重和干重均增加,人参皂苷含量提高10%左右。在去Ca2+培养基上生长的人参愈伤组织细胞,经回转器水平回转3周后,人参皂苷含量约为正常重力条件下培养细胞的2倍。另外,在试验范围内,如果培养基中起始钙离子浓度越高,则其培养的人参细胞中人参皂苷含量越低。

Objective:To express human interferon-α2b gene and to explore the feasibility of expressing human gene in plant cells.Methods:The hIFN-α2b coding sequence was amplified by PCR with specific primers and plasmid pBV889 was used as a template,subcloned into middle vector pMD18-T and binary vector pBI121 to obtain plant expression vector pBIFN. The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-α2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation....

Objective:To express human interferon-α2b gene and to explore the feasibility of expressing human gene in plant cells.Methods:The hIFN-α2b coding sequence was amplified by PCR with specific primers and plasmid pBV889 was used as a template,subcloned into middle vector pMD18-T and binary vector pBI121 to obtain plant expression vector pBIFN. The pBIFN was transformed into Agrobacterium tumefaciens strain LBA4404. Then hIFN-α2b gene was introduced into Ginseng callus cells via Agrobacterium-mediated transformation. The positive cells were screened by G418. The transgenic Ginseng calli were confirmed by PCR,RT-PCR,Western blot and WISH/VSV system.Results:Stable integration of the hIFN-α2b gene in the Ginseng callus cells′ genome was confirmed by PCR analysis. RT-PCR analysis showed that there were transcription products. Western blot implied that the given protein was hIFN-α2b. WISH/VSV system assay showed that the expressed hIFN-α2b possessed relatively lower bioactivity.Conclusion:HIFN-α2b has been expressed in transgenic Ginseng calli, which facilitates further investigation of improving the curative effect of orally administered hIFN-α2b.

目的:利用组织培养人参愈伤组织细胞表达人干扰素,探讨用植物细胞表达人类基因的可行性。方法:用PCR从pBV889扩增人干扰素α2b(hIFN-α2b)编码基因,将其克隆于pMD18-T载体和pBI121,进而构建植物细胞表达载体pBIFN。用冻融法将pBIFN导入根瘤农杆菌LBA4404,经卡那霉素和利福霉素筛选后利用农杆菌介导的转化法将hIFN-α2基因导入人参愈伤组织细胞。经G418筛选,形成阳性细胞。用PCR、RT-PCR、Western blot和WISH/VSV系统检测转基因组织。结果:经PCR检测表明hIFN-α2b基因已成功整合到人参愈伤组织细胞基因组中。RT-PCR证明存在hIFN-α2b基因的转录产物。Western blot分析表明转基因人参愈伤组织细胞能有效表达hIFN-α2b蛋白。WISH/VSV系统检测分析表明表达的hIFN-α2b具有生物学活性。结论:本研究利用转基因人参愈伤组织细胞成功地表达了人干扰素,为进一步提高口服干扰素的疗效打下基础。

 
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