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粪样细菌区系
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  “粪样细菌区系”译为未确定词的双语例句
     Tracking of the development of fecal bacterial community of diarrhea piglets by 16S rDNA techniques
     16S rDNA技术研究新生腹泻仔猪粪样细菌区系的多样性变化
短句来源
     DIVERSITY OF FECAL BACTERIAL COMMUNITY OF NEWBORN PIGLET DIARRHEA AS REVEALED BY THE 16S rDNA TECHNIQUES
     16S rDNA技术跟踪研究新生腹泻仔猪粪样细菌区系的多样性变化
     This thesis selected lactulose as substitute for antibiotic, influence of immunity&fecal bacterial flora of lactulose on calves in perinatal period has been researched, the effect of lactulose in milk for calves' health and growth function has been appraised.
     本课题选用乳果糖为替抗生素物质,主要研究处于围产期的犊牛乳料中添加乳果糖对犊牛免疫功能及粪样细菌区系的影响,从而评价乳果糖作为替抗生素物质添加到犊牛乳料中对犊牛健康及生长发育的作用。
短句来源
     Development of Bacterial Community in Faeces of Weaning Piglets as Revealed by Denaturing Gradient Gel Electrophoresis
     变性梯度凝胶电泳法研究断奶仔猪粪样细菌区系变化
短句来源
     Influence of Lactulose of Immunity & Fecal Bacterial Flora on Calves in Perinatal Period
     乳果糖对围产期犊牛免疫功能及粪样细菌区系的影响
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  相似匹配句对
     APXIVA gene couldn't be detected from dung.
     中不能检测到该基因。
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     Feces Analysis and Its Role in Wildlife Research
     在野生动物研究中的作用
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     Sample Test
     (题)
短句来源
     Analysis of calves' excrement with Denaturing Gradient Get Electrophoresis(DGGE);
     细菌区系的变性梯度凝胶电泳(DGGE)分析;
短句来源
     Influence of Lactulose of Immunity & Fecal Bacterial Flora on Calves in Perinatal Period
     乳果糖对围产期犊牛免疫功能及细菌区系的影响
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PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the deve- lopment of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated...

PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the deve- lopment of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated and diverse. Each individual piglet had its unique DGGE profile, with low similarity between piglets. The source of sow and the diet showed no appa- rent effect on the DGGE profiles. For the piglets fed a diet containing oliogofructose, DGGE similarity analysis showed bacterial community in the faeces developed fast during the first week and relatively slow later after weaning.

利用PCR和DGGE技术分析了 1 2头仔猪在断奶后其粪样细菌区系的变化。粪样细菌 1 6SrDNA的V6~V8可变区经PCR扩增 ,扩增产物经DGGE电泳后再进行相似性分析。结果表明 ,仔猪断奶当天粪样DGGE谱带少 ,同窝仔猪间图谱相似。断奶后 ,随着断奶时间的推移 ,每头仔猪的DGGE图谱带逐渐增多 ,变得复杂和多样 ,仔猪个体间DGGE图谱差异逐渐增大。仔猪是否同窝以及所采食日粮类型对DGGE图谱没有明显影响。相似性分析还表明 ,日粮中添加寡果糖的仔猪在断奶后第 1周 ,其粪样微生物区系变化迅速 ,而后缓慢。

PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets.Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.Results revealed that DGGE profiles from fecal samples of 5 piglets changed form simple to complex,and then...

PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets.Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.Results revealed that DGGE profiles from fecal samples of 5 piglets changed form simple to complex,and then returned simple to complex,finally trended to be stable and diverse.DGGE profiles from day 2and 16 fecal samples of 5 piglets were simplest,with high similarity(84%).Their most predominant bands were E.coil.Their Shannon index were 1.38±0.55 and 1.97±0.26 respectively.DGGE profiles from day 10 of 5 piglets became quite complex,and had quite low similarity(10%) with DGGE profiles from day 2and 16 fecal samples of 5 piglets.The Shannon index was 2.12±0.24.E.coil still existed,but it was not the most predominant band.There were significant differences of predominant bands between DGGE profiles from day 27 fecal samples and those from day 35 and 42 fecal samples,with 26.28% and 39.2% of similarity respectively.The predominant bands from day 35 and 42 fecal samples of 5 piglets were trended to be stable,with 55% of similarity.The Shannon index of day 27,day 35 and 42 fecal bacterial communities were 2.19±0.29,2.20±0.17and 2.14±0.20 respectively.The fecal bacterial communities of day 42 health piglets were mainly consisted of species of Enterococcus,Streptococcus,Clostridium,Peptostreptococcus and Lactobacillus.

用PCR/DGGE技术跟踪一窝5头新生腹泻仔猪自然康复、补饲、断奶过程中粪样细菌区系的演变,构建3头仔猪42日龄粪样的16S rDNA克隆库,分析匹配于DGGE优势谱带23个克隆的16S rDNA序列。结果表明,DGGE图谱由简单(2日龄)到复杂(10日龄),再回复简单(16日龄)到复杂(断奶),最后趋于稳定。2、16日龄DGGE图谱最简单、相似,最优势谱带为大肠杆菌;10日龄(补饲后3天)图谱复杂,大肠杆菌存在但不是最优势谱带,补饲前后图谱的相似性低,补饲导致了粪样细菌区系结构的显著变化;断奶前(27日龄)和后(35、42日龄)图谱复杂,优势谱带、图谱相似性均趋向稳定。序列分析表明,23个克隆中除5个与未知细菌最相似外,其余最相似菌分属于肠球菌(Enterococcus),链球菌(Streptococcus),梭菌(Clostridium),消化链球菌(Peptostreptococcus)和乳酸杆菌(Lactobacillus)。

 
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