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   固定化酶 在 一般化学工业 分类中 的翻译结果: 查询用时:1.539秒
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固定化酶     
相关语句
  immobilized enzyme
    Study on the Production of Fructooligosaccharide by Immobilized Enzyme
    固定化酶法合成蔗果低聚糖的研究
短句来源
    Study on production of casein phosphopepfides by Immobilized Enzyme
    固定化酶生产活性肽CPP研究
短句来源
    The optimal pH for immobilized enzyme and the free one are pH9.6 and9.0 respectively. Its stability is improved obviously and its half life period is 7.5 months.
    固定化酶和游离酶的最适pH分别为9.6和9.0,固定化后,其酶的稳定性明显提高,半衰期为七个半月。
短句来源
    In comparison with the free enzyme, the temperature to loss a half activity of the immobilized enzyme was increased from 47℃ to 100℃,the optimum temperature and optimum pH were shifted from 40℃ to 80℃ and from 6 to 5.5, respectively. The Km and K′m value of immobilized enzyme were 50 mg/ml and 56 mg/ml, respectively.
    固定化酶的半失活温度比游离酶的高 ,半失活温度由游离酶的 4 7℃提高到 10 0℃ ,最适反应温度由 4 0℃上升至 80℃ ,最适pH由 6下降到 5 .5 ,固定化酶K′m值由游离酶的Km 5 0mg/mL增加到 5 6mg/mL。
短句来源
    Besides, the optimal reaction time was 18 hour for immobilized enzyme changing maltose to trehalose and we can acquire the highest content of trehalose.
    另外 ,固定化酶转化麦芽糖为海藻糖的最佳反应时间为 18h ,这时可以获得最高含量的海藻糖
短句来源
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  immobilized enzymes
    The immobilized enzymes were stable at 60℃ but deactivated at 70℃ for about 5 minutes.
    固定化酶在60℃稳定,在70℃5分钟失活。
短句来源
    The effects of the quantity of glutamic acid and electrical density of the layered sheet on immobilized enzymes activities are discussed.
    讨论了谷氨酸插层量和层板电荷密度对固定化酶活力的影响。
短句来源
    The immobilized enzymes was storage stable in water and operational stable for batch reaction, revealled its potential capcity of application in maltose industry.
    固定化酶在水中贮藏稳定,并具有良好的操作稳定性,使其在麦芽糖生产中具有潜在的应用价值。
短句来源
  immobilized lipase
    The experiment first filtered all kinds of carriers, selected alkalescence anion exchange resin D301-G as the carrier immobilizing lipase, and optimized the immobilizing condition of D301-G: absorption 8h in 13 ℃ shake bed. the absorption time is 8h, enzyme activity is 127IU/g, the concentration of Glutaraldehyde solution is 1%, the time of crosslinking is 10min. The enzyme activity of immobilized lipase is 28.0 IU/g(average).
    本课题首先筛选了各种载体,选择弱碱性阴离子交换树脂D301-G树脂作为固定化猪胰脂肪酶的载体,并且确定了D301-G作为固定化载体的优化条件:在13℃的摇床中,吸附时间为8h,给酶量为127IU/g,戊二醛浓度1%,交联时间10min,得到固定化酶活为28.0 IU/g(平均值)。
短句来源
    The content of EPA/DHA of structure lecithin was 6.83%, the content of EPA and DHA was respectively 1.40% and 5.43%. The immobilized lipase D301-G is of no effect to the transesterification of lecithin with fish oil.
    得到的产物卵磷脂中含有EPA为1.40%,DHA为5.43%,总的EPA/DHA的含量为6.83%,而固定化酶D301-G对这个催化这个反应没有效果。
短句来源
    The results showed that the maximum 0.44U/cm 2activity of the immobilized lipase was given when the benzoquinone content was 0.01g/ml,activation time was 60min,lipase content in pH 8.0 phosphate buffer was 0.005g/ml and crosslinking reaction time was 24h at 4℃.
    结果表明,当对苯醌浓度为0.01g/ml,活化60min,与浓度为0.005g/ml的酶的pH8.0的磷酸盐缓冲溶液于4℃交联24h,获得的固定化酶活最高,为0.44U/cm2。
短句来源
    The optimum operating temperature and pH of the immobilized lipase was 35℃ and 9.0 respectively.
    固定化酶最适温度35℃,最适pH9.0。
短句来源
    When the amount of lipase is 400u/(g CADB)~1200u/(g CADB),the activity of immobilized lipase is 193u/(g CADB)~448u/(g CADB)and the activity recovery is above 37%.
    加酶量为 40 0 u/g~ 1 2 0 0 u/g(载体 )时 ,所得固定化酶活力为 1 93u/g~ 448u/g(载体 ) ,此时固定化酶的活力回收率较高 (>37% )。
短句来源
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  “固定化酶”译为未确定词的双语例句
    The removal efficiencies of 2, 4-dichlorophenol (1 mmol/L) were all above 99.5% with the concentration of 0.5mmol/L chloride ions released during repeated 8 batch reactions.
    使用柱式固定化酶反应器处理2,4-二氯酚,在批式反应工艺条件下,当底物浓度为1 mmol/L、反应3-5h,2,4-二氯酚的去除率可达99.5%以上(脱除的氯离子浓度达0.5 mmol/L)。
短句来源
    Finally. The degradability of enzyme on 1% chitosan solution was tested in a reactor (Φ14.5cm×8.5cm) . As the result showed, when flow velocity was at 5mL/min, chitosan could be degraded effectively.
    在固定化酶反应器(Φ14. 5cm×8. 5cm)中测定了不同流速下固定化酶对1%壳聚糖溶液的降解情况,结果表明,大分子量壳聚糖在流速为5mL/min时,能够降解得特定分子量的壳聚糖产品。
短句来源
    The activity of 12.5u/g. enzyme beads could be acheived using 20% solution of acrylamide. The optimum temperature and pH and thermostability of β-galactosidase was negligibly affected by immobilization.
    此乳糖酶能够成功地固定在丙烯酰胺凝胶中,以20%聚丙烯酰胺浓度的凝胶可制得活力为12.5u/g湿酶粒的固定化酶,固定化后最佳pH、最适温度及热稳定性均不受影响,并且游离酶与固定化酶的动力学常数具有可变性。
短句来源
    The Production of Isomaltooligosaccharides by Immobilized Aspergillus Niger or Immobilized α-Transglucosidase
    固定化细胞法和固定化酶法生产低聚异麦芽糖
短句来源
    The activity of enzyme was not destroyed, and the immobilized cellobiase were quite stable with a long half-life.
    该固定化酶在纤维素酶的协同降解过程中具有明显的促进作用。
短句来源
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  immobilized enzyme
The catalytic constant (25 s-1) andKM (0.17 mM) for the immobilized enzyme for the hydrolysis of N-acetyl-L-tyrosine ethyl ester are comparable to the corresponding characteristics for the free enzyme.
      
Thermal inactivation of the immobilized enzyme under the reaction conditions was studied.
      
The kinetics of acidic inactivation of the native and immobilized enzyme was studied.
      
The immobilized enzyme was more resistant to temperature and pH.
      
Methods for stabilization of the immobilized enzyme were developed, and some kinetic parameters of the immobilized preparations were determined.
      
更多          
  immobilized enzymes
Enzymatic methods for the determination of mercury(II) using native and immobilized enzymes from different classes and sources were considered.
      
The catalytic properties of free and immobilized enzymes were compared.
      
Computer Simulation of Porous Electrodes with Immobilized Enzymes: The Percolation Properties of Multicomponent Structures
      
The percolation characteristics of porous electrodes with immobilized enzymes are calculated.
      
Porous Electrodes with Immobilized Enzymes: The Problem of Development of Nanocomposites with High Concentrations of Molecules o
      
更多          
  enzyme immobilization
Emphasis will be placed on recent biological and biomedical developments and trends such as enzyme immobilization, cell isolation, protein purification, target drugs and DNA separation.
      
The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed.
      
In the other, a certain type of enzyme immobilization on the support is required.
      
Two kinds of disperse carbonaceous materials-activated carbon NORIT and carbon black PM-100, were used as matrices for enzyme immobilization.
      
The pH of the solution affected the adsorption capacities of the selected immobilization matrices; larger amounts of CAT adsorbed were estimated in neutral and alkaline solutions than under acidic conditions for enzyme immobilization.
      
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  其他


The permeability of the cell membrane of yeast is the main factor whic imits the enzymic phosphorylation synthesis of ATP when yeast used. It h is obvious that different pertreatment has diffevent effect on permeability of cell membrane for different strains of yeast. The membrane permeabilitie -s of the beer yeast soaked by cold water and the bread yeast dried by the sunlight are so slightly increased that the enzymatic phosphorylation synthe -sis of ATP can carry out within the cells. Therefore this will provide...

The permeability of the cell membrane of yeast is the main factor whic imits the enzymic phosphorylation synthesis of ATP when yeast used. It h is obvious that different pertreatment has diffevent effect on permeability of cell membrane for different strains of yeast. The membrane permeabilitie -s of the beer yeast soaked by cold water and the bread yeast dried by the sunlight are so slightly increased that the enzymatic phosphorylation synthe -sis of ATP can carry out within the cells. Therefore this will provide a good condition for the cell to be immobilized for produsing ATP in industr -y. The membrane permeability of the stoved bread yeast and the frozen white wine yeast are too greatly increased to keep the enzymatic phosphorylation to take place neither in nor out of the cells. The expeiment alro proves that glucose can faci-litate the release of hexokinase from the white wine yeast cell. Thus we can extract enzyme liguid with glucose, or make further immobilized enzyme system for broducing ATP industrially.

酵母用于酶促磷酸化合成ATP主要受酵母细胞膜渗透性限制。显然,不同的预处理对不同菌株酵母细胞膜的渗透性有不同的影响。冷水浸洗的啤酒酵母和日晒干燥的面包酵母,其细胞膜的渗透性稍有提高,酶促磷酸化反应可在细胞内进行。因而可提供固定化细胞的条件,用于工业生产ATP。烘干的面包酵母和冰冻的白酒酵母,其细胞膜的渗透性大幅度增加,致使酶促磷酸化反应既不能在细胞内,也不能在细胞外进行,实验还证明,葡萄糖对白酒酵母细胞中的已糖激酶释放有促进作用,因而可用以提取酶液,或进一步制成固定化酶,工业生产ATP。

A kind of protein-like material was used as the carrier for preparing immobilized microbial cells containing glucose isomerase activity by using cell-carrier co-cro-sslinking method. Defferent crosslink conditons were trailed. The final product shows a good Property in continuous column isomerization. The yield of the immobilzed GI preparation is 3.6 grams per 100 mililiter broth and productivity is 6.6 kg converted glucose per gram of immobilized enzyme. Some properties and characteristics of the GI prepartion...

A kind of protein-like material was used as the carrier for preparing immobilized microbial cells containing glucose isomerase activity by using cell-carrier co-cro-sslinking method. Defferent crosslink conditons were trailed. The final product shows a good Property in continuous column isomerization. The yield of the immobilzed GI preparation is 3.6 grams per 100 mililiter broth and productivity is 6.6 kg converted glucose per gram of immobilized enzyme. Some properties and characteristics of the GI prepartion wore also reported.

本文介绍用细胞-载体共交联法制备固定化葡萄糖异构酶的方法。有机及无机类载体具有良好的交联特性。将其与含葡萄糖异构酶的游动放线菌菌体混合,在一定条件下用戊二醛交联,可制备具有良好性能的固定化酶。应用正交试验法,选出了适合于该载体的交联条件。因此细胞-载体共交联法制备的固定化酶,得率比无载体法提高三倍,生产成本降低约60%。该固定化酶的柱式连续转化最高活性为6700u/g。异构化生产能力为6.6公斤/每克绝干固定化酶。对该固定化酶的一般酶学性质及工作性能在本文中也做了介绍。

It has boon investigated to continuously isomerize glucose to fructose with Actino-planes missourionsis Ao-S1-2 fixed cells directly cross linked by glutaraldhyde. The im-portent advantage of this enzyme are simple handling and operating, suited for the iarge scale production. of high fructose syrup.It has a productivity of 3.75T DS in single column packated 20-30 Kg enzyme.The productivity are lass effected by tested range of glucose syrup purity.It is rocommanded to operat with series column.It is better than...

It has boon investigated to continuously isomerize glucose to fructose with Actino-planes missourionsis Ao-S1-2 fixed cells directly cross linked by glutaraldhyde. The im-portent advantage of this enzyme are simple handling and operating, suited for the iarge scale production. of high fructose syrup.It has a productivity of 3.75T DS in single column packated 20-30 Kg enzyme.The productivity are lass effected by tested range of glucose syrup purity.It is rocommanded to operat with series column.It is better than single column or parallel column. There are stable flow and high productivity (1:5916).

本文研究了用戊二醛直接交联的游动放线菌(Actinoplanes messouriensis)Ac-81-2的固定化细胞转化葡萄糖为果糖的异构化工艺。通过试验表明该固定化酶的突出优点:操作简单、容易掌握、适合于大规模生产高果糖浆。装填20-30公斤固定化酶的单柱生产能力可达3.75吨糖/公斤酶(即1∶3750)。且对在试验范围内的原料糖浆的纯度不是很敏感。通过对连续异构化的单柱、并联、串联比较,以串联为好。流量稳定,生产能力高(1∶5916)。 评价固定化酶的指标虽然很多,但根本的指标应是异构化能力。即每公斤或每克固定化酶,在使用寿命内将葡萄糖异构化为果葡糖(果糖含量42%,目前国内常指转化率42%)的总量,称为生产能力。全世界用得最广泛的固定化葡萄糖异构酶是丹麦Novo公司生产的Sweetzyme Q,占全世界用量的70%,Sweetzyme Q每公斤能转化2.5吨糖(绝干)。另外还有美国Miles公司的Talcesweet及荷兰Gist Brocades公司生产的Maxazyme-GI-Immob亦用得较多。近年来西德Miles Kalie-chemi公司生...

本文研究了用戊二醛直接交联的游动放线菌(Actinoplanes messouriensis)Ac-81-2的固定化细胞转化葡萄糖为果糖的异构化工艺。通过试验表明该固定化酶的突出优点:操作简单、容易掌握、适合于大规模生产高果糖浆。装填20-30公斤固定化酶的单柱生产能力可达3.75吨糖/公斤酶(即1∶3750)。且对在试验范围内的原料糖浆的纯度不是很敏感。通过对连续异构化的单柱、并联、串联比较,以串联为好。流量稳定,生产能力高(1∶5916)。 评价固定化酶的指标虽然很多,但根本的指标应是异构化能力。即每公斤或每克固定化酶,在使用寿命内将葡萄糖异构化为果葡糖(果糖含量42%,目前国内常指转化率42%)的总量,称为生产能力。全世界用得最广泛的固定化葡萄糖异构酶是丹麦Novo公司生产的Sweetzyme Q,占全世界用量的70%,Sweetzyme Q每公斤能转化2.5吨糖(绝干)。另外还有美国Miles公司的Talcesweet及荷兰Gist Brocades公司生产的Maxazyme-GI-Immob亦用得较多。近年来西德Miles Kalie-chemi公司生产的Opfisweet2_2每公斤酶能转化22吨糖,水平很高但价格昂贵,未见大规模使用的报道。我国经过十年来的研究已批量生产。本文报道以戊二醛直接交联游动放线菌Actinoplaaes messourieasis Ac-81-2的固定化细胞连续异构化葡萄糖为果葡糖 (?

 
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