It indicates that appropriate number of inoculation of explant embryo is about 6 seeds when the volume of culture medium is 15-20 ml It also reports that regeneration frequency has been increased by properconcentration of NAA(2mg/L) and additional some yeast extract in MS culture medium
The inducingand selecting technique and hereditary variation character of cold resistance variant ofJaponica Rice is reported,the application situation of new rice varietie bred by tissue culture is also reported.