|
|
|
|
|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
|
|

| | In this article data on the effect of spacers on the efficiency of coupling of ligands to agarose matrix and the retention of ligand activities were presented. Two diamines, viz ethylenediamine (EDA) and iminobispropylamine (IBPA) were employed as spacers which were separately coupled to Sepharose CL-6B beads activated with trichloro-s-triazine (TsT). These spacer-bearing Sepharose CL-6B were further activated with glutaraldehyde and coupled with either monoclonal or polyclo-nal antibodies. The results indi... | | 本文研究了两种二胺类间隔臂EDA、IBPA在免疫亲和层析中的应用。结果表明,与无间隔臂的支持物(DST-支持物)比较,间隔臂的引入可大幅度降低固相抗体(单克隆和多克隆)活性的损失。这项工作可供免疫吸附剂的设计及固相配体性质的研究作为参考。 | | 文摘来源 | | Antibodies raised against tumour-associated antigens have been assessed tor tumour specificity using an indirect immunohistochemical peroxidase staining of paraffin-embedded tissue from colon carcinoma, colon adenoma with dysplasia and normal mucosa. Unabsorbed polyclonal antibody to carcinoembryonic antigen (CEA), monoclonal antibodies to CEA (Mab 3851 and Mab 27), monoclonal antibodies to protein-bound carbohydrates (Mab C 216 and Mab C 242) and to lipid-bound carbohydrates (Mabs C 50 and 19-9) were used ... | | 本文应用结肠癌、伴异型增生的结肠腺瘤及正常结肠粘膜组织的石蜡切片作间接法免疫过氧化酶染色,以测定一批肿瘤伴随抗原的抗体的肿瘤特异性。所用抗体包括:抗癌胚抗原(CEA)的多克隆抗体及单克隆抗体Mab3851、Mab27;抗蛋白质—碳水化合物的单克隆抗体MabC216、MabC242;抗脂质—碳水化合物的单克隆抗体MabC50、MabC19-9。实验结果发现三个单克隆抗体(Mab3851、Mab27及MabC216)的肿瘤特异性与正常对照有显著性差异。其增高的染色频率也见于异型增生的粘膜。而MabC242则逊於前三者。抗CEA的多克隆抗体、单克隆抗体MabC50及Mab19-9无肿瘤特异性。结果表明前三个抗体在鉴别结肠粘膜的良恶性质上,尤其在识别癌前病变上,具有一定的参考价值。 | | 文摘来源 | | Transforming growth factor-β(TGF-β)and bone morphogenetic protein(BMP)were related to embryonic development and differentiation of cells. In this study,the expression and distribution of TGF-βand BMP in 60 cases of salivary gland tumors were studied by using immunohistochemistry.The results showed that the staining patterns of TGF-βand BMP were similar,but the fibrous matrix of tumors was pos-itive to TGF-β,not to BMP.Some well-differentiated malignant salivary gland tumors such as acinic cell car- cinoma,a... | | 转化生长因子β(TGF-β)和骨形成蛋白(BMP)与胚胎发育、细胞分化密切相关.本研究利用多克隆及单克隆抗BMP抗体、单克隆抗TGF-β抗体,通过免疫组化方法,初步观察了11类60例各种唾液腺肿瘤中TGF-β,BMP的表达与分布.结果发现,TGF-β与BMP的分布模式基本一致,只是TGF-β在肿瘤纤维组织基质中呈弥漫性轻度阳性,而BMP则为阴性.多形性腺瘤、基底细胞腺瘤、肌上皮瘤等良性唾液腺肿瘤中TGF-β,BMP呈强阳性,而腺癌、乳头状囊腺癌、腺样囊性癌等恶性唾液性肿瘤中则呈弱阳性.因此,TGF-β,BMP的表达可能与唾液腺肿瘤的发生和细胞分化有关. | | 文摘来源 | |   | | << 更多相关文摘 |
|
相关查询: | |
对查询结果不满意
|
|
|
|