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酸性植酸酶
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  acidic phytase
     The objective of this research is to screen out the strain which can produce acidic phytase at higher level and clone its phytase gene, thereby providing the gene of interest for genetic engineering on phytase.
     本实验的目的旨在从土壤中筛选出酸性植酸酶高产菌株,并克隆其植酸酶基因,从而为植酸酶的基因工程提供基因材料。
短句来源
  “酸性植酸酶”译为未确定词的双语例句
     Studies on Expression of Acid Phosphorutase phyB Gene in Pichia pastoris GS115
     酸性植酸酶phyB在毕赤酵母GS115中的表达
短句来源
     Studies on Expression of Acid Phosphorutase phyB Gene in Pichia Pastoris GS115
     酸性植酸酶phyB基因在巴斯德毕赤酵母GS115中的表达研究
短句来源
     The phytase produced in seeding is at the most adapt PH 5.0, the most adapt temperature 45℃.
     植物种子萌发时 ,其幼苗生产的酸性植酸酶的最适PH值在 5.0附近 ,最适温度在 45℃附近。
短句来源
     The Screening of an Acidic Phytase-producing Strain and the Cloning of Its Phytase Gene
     酸性植酸酶产生菌的筛选及其基因克隆
短句来源
     Molecular Cloning and Expression of Acid Phosphotase Gene in E.coli and Pichis Pastoris
     酸性植酸酶基因的克隆及其在大肠杆菌和毕赤酵母中的表达
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  相似匹配句对
     Acid Deposition
     酸性降水
短句来源
     The Screening of an Acidic Phytase-producing Strain and the Cloning of Its Phytase Gene
     酸性植酸酶产生菌的筛选及其基因克隆
短句来源
     Studies on Expression of Acid Phosphorutase phyB Gene in Pichia pastoris GS115
     酸性植酸酶phyB在毕赤酵母GS115中的表达
短句来源
     Studies on the Acidity of Zeolite Y
     Y沸石的酸性
短句来源
     A study of phytase and its application
     植酸酶及其应用
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The phytase produced in seeding is at the most adapt PH 5.0, the most adapt temperature 45℃. The phytase is extensive adept to ions. The phytase is activated by Ca and inhibited Cu and Zn. The concentration of Cu and Zn are about 0.8mmol/L and 1.0mmol/L when phytase activation of 50% is inhibited. The phytase is maintained activation longer in PH 5.0.

植物种子萌发时 ,其幼苗生产的酸性植酸酶的最适PH值在 5.0附近 ,最适温度在 45℃附近。酶对离子的适应范围较宽。Ca离子对酶活性有明显的激活作用 ,Cu和Zn离子对酶活性有明显的抑制作用 ,抑制 50 %酶活性的Cu和Zn离子的浓度分别为 0 .8mmol/L和 1 .0mmol/L。酶应用的最好条件应该在PH值 5.0 ,温度 30℃左右

Pichia pastoris has been developed to be a very efficient expression host for the heterogeneous proteins since its alcohol promoter was isolated and cloned, and its transformation technique was established. For further improving the secretion expression of heterogeneous proteins, in this research, the signal sequences were studied. At first, the Saccharomyces cerevisiae mating factor α prepro-leader sequence was synthesized using successive PCR and designated as MF4I. Then, ten different signal sequences were...

Pichia pastoris has been developed to be a very efficient expression host for the heterogeneous proteins since its alcohol promoter was isolated and cloned, and its transformation technique was established. For further improving the secretion expression of heterogeneous proteins, in this research, the signal sequences were studied. At first, the Saccharomyces cerevisiae mating factor α prepro-leader sequence was synthesized using successive PCR and designated as MF4I. Then, ten different signal sequences were constructed by adding the N-terminal residues of Pichia pastoris Aox1 protein to the N-terminal of the MF4I. These ten signal sequences were used for directing phytase gene secretion in Pichia pastoris, the secretion of phytase were increased in Pichia pastoris strains containing new signal sequence. Among these strains, the phytase secretion was highest in strain contain signal sequence added with A, I, P three Aox1 N-terminal residues; the phytase secretion of Pichia pastoris was 90 mg/L in flake. The secretion was six-fold than that with original Saccharomyces cerevisiae mating factor α prepro-leader sequence. In addition, insert of ten residues E E A E A E A E P K can further increase the phytase secretion by 35%, the secretion reach 120 mg/L.

乙醇氧化酶启动子被分离、克隆 ,并建立了转化方法后 ,毕赤酵母已被发展成为一种高效的外源蛋白表达宿主。为了进一步提高外源蛋白质的分泌表达 ,对信号肽序列进行了研究。首先按毕赤酵母的偏爱密码合成了酿酒酵母的α因子信号肽序列MF4I,随后在MF4I信号肽序列的N端分别引入 1~ 10个毕赤酵母Aox1蛋白质的N端氨基酸 ,构成 10种不同的分泌信号肽序列 ,10种不同的分泌信号肽序列被用于植酸酶基因的毕赤酵母分泌表达。以上新的信号肽序列都可使植酸酶的分泌表达量增加 ,而以N端增加A、I、P三个氨基酸的信号肽序列引起的提高最大 ;和野生型的酿酒酵母α因子信号肽序列相比 ,使植酸酶分泌表达量增加 5倍 ,摇瓶中植酸酶的分泌表达量为 90mg/L。此外在MF4I信号肽的引导序列和内切蛋白酶间增加了EEAEAEAEP和K共 10个氨基酸 ,进一步提高信号肽的分泌效率 ,使表达又提高约 35 % ,使得摇瓶中酸性植酸酶的表达量达到 12 0mg/L ,是pPCI9K表达量的 8倍。

The properties of phytase from A. niger 4961 were studied. The results showed that, two phytases existed in the extract of A.niger 4961; the optimal pH, the optimal temperature, and the molecular weight of the two phytases were 6.0,3.0, 40 ℃,55 ℃,and 64 200,41,100, respectively.

黑曲霉(A.niger)496 1菌株所产胞外植酸酶,经透析浓缩、DEAE cellulose离子交换层析及SephadexG 75,Sephacryl100凝胶过滤,获得了2个植酸酶,其相对分子质量分别为64200及41100.该2个植酸酶的最适pH分别为6.0和3.0,最适温度分别为55℃和40℃.初步确定一个是中性植酸酶,另一个为酸性植酸酶.

 
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