助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   糖蛋白gi 的翻译结果: 查询用时:0.419秒
图标索引 在分类学科中查询
所有学科
生物学
畜牧与动物医学
更多类别查询

图标索引 历史查询
 

糖蛋白gi
相关语句
  glycoprotein i
     Cloning and Expression of Glycoprotein I Gene of Marek′s Disease Virus Strain G2
     马立克氏病病毒广西株G2囊膜糖蛋白gI基因的克隆和表达
短句来源
     SEQUENCE ANALYSIS OF GLYCOPROTEIN I GENE OF MAREK′S DISEASE VIRUS CVI988
     马立克氏病病毒疫苗CVI988株囊膜糖蛋白gI基因的序列分析
短句来源
     Cloning and Expression of Glycoprotein I Gene of Marek′s Disease Virus Strain 648
     马立克氏病病毒648株囊膜糖蛋白gI基因的克隆和表达的研究
短句来源
     Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).
     通过聚合酶链式反应 (PCR) ,扩增出马立克氏病病毒特超强毒 (vv +MDV) 648株囊膜糖蛋白gI基因 ,并将该基因按正确的阅读框 (ORF)克隆到表达性载体质粒pGEX 6P 1中谷胱甘肽转移酶 (GST)基因的下游。
短句来源
     CLONING AND SEQUENCE ANALYSISING OF GLYCOPROTEIN I (gI) GENE OF MAREK′S DISEASE VIRUS FOR DIFFERENT STRAINS
     不同毒株马立克氏病病毒囊膜糖蛋白gI基因的克隆和序列分析
短句来源
更多       
  “糖蛋白gi”译为未确定词的双语例句
     STUDIES ON IN VITRO EXPRESSION FOR gI GENE OF MAREK'S DISEASE IN E.COLI BY pGEX VECTOR
     pGEX载体表达马立克氏病病毒囊膜糖蛋白gI基因的最佳条件
短句来源
     The results showed that fusion protein G1S0.7 could be recognized by both the hantavirus NP and glycoprotein Gl specific mAbs, and its molecular weight was consistent with the predicted full one on Western blot;
     其中GISO.7融合蛋白可同时被抗汉坦病毒NP及糖蛋白GI特异性单抗所识别,免疫印迹结果显示该蛋白分子量与预期完整融合蛋白大小相一致;
短句来源
  相似匹配句对
     The difference of germination percentages among Gi.
     Gi.
短句来源
     versiforme and Gi.
     versiforme及Gi.
短句来源
     Molecular Characterisation of Marek's Disease Virus Glycoprotein I Gene
     马立克氏病病毒囊膜糖蛋白gI基因的分子生物学特性
短句来源
     Duck CRP is a glycoprotein.
     鸭CRP是糖蛋白
短句来源
     Cloning and Expression of Glycoprotein I Gene of Marek′s Disease Virus Strain G2
     马立克氏病病毒广西株G2囊膜糖蛋白gI基因的克隆和表达
短句来源
查询“糖蛋白gi”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  glycoprotein i
None had IgG higher than 80 GPL units or was positive for anti-β2 glycoprotein I.
      
In contrast to young patients, elderly patients with 10 or more GPL units aCL and negative for anti-β2 glycoprotein I do not seem to have a higher risk of vascular events.
      
They include lupus anticoagulants (LA), anticardiolipin (aCL) antibodies, and the most recently recognized anti-beta-2-glycoprotein I (β2-GPI) and antiprothrombin (aPT) antibodies.
      
The genetics of β2-glycoprotein I, one of the most representative target antigens of aPL, has been extensively studied.
      
Association of the platelet membrane glycoprotein I a C807T gene polymorphism with aspirin resistance
      
更多          


Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).PCR product was cloned into pGEX\|6p\|1 according to the right open reading frame(ORF).The expression of GST\|gI fusion protein was studied in detail on many factors including temperature,timing and IPTG.The curve for OD 600 and the growing time of the recombinant bacteria is also established.,which is helpful to find the optimal inducing time.GST\|gI fusion protein was identified...

Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).PCR product was cloned into pGEX\|6p\|1 according to the right open reading frame(ORF).The expression of GST\|gI fusion protein was studied in detail on many factors including temperature,timing and IPTG.The curve for OD 600 and the growing time of the recombinant bacteria is also established.,which is helpful to find the optimal inducing time.GST\|gI fusion protein was identified by SDS\|PAGE and Western\|blotting., and the result shows that the best concentration of IPTG is 0.2~0.5mmol/L and inducing time have great effects on expression while temperature has little.The fusion protein was injected into mouse five times to identify its antigenicity and the result is positive in indirect fluorescent assay IFA.

通过聚合酶链式反应 (PCR) ,扩增出马立克氏病病毒特超强毒 (vv +MDV) 648株囊膜糖蛋白gI基因 ,并将该基因按正确的阅读框 (ORF)克隆到表达性载体质粒pGEX 6P 1中谷胱甘肽转移酶 (GST)基因的下游。重组质粒 (pGEX gI)经氯化钙转化宿主菌BL2 1 ;通过建立重组菌生长时间与OD60 0 值间的关系曲线 ,以及对诱导时间、诱导温度、IPTG浓度等条件的摸索 ,根据聚丙烯酰胺凝胶电泳 (SDS PAGE)判定GST gI融合蛋白的最佳表达条件 ,并经蛋白质印迹试验 (WesternBlotting)对表达产物进行了验证。将表达产物免疫小鼠 ,所得抗血清能与MDV感染的鸡胚成纤维细胞 (CEF)在间接免疫荧光试验 (IFA)中 ,呈较强的细胞膜荧光着色。实验结果表明 :IPTG的最佳浓度为 0 2~ 0 5mmol L ;最适的诱导时期为重组菌生长对数中期 ;温度对表达几乎没有影响。pGEX载体表达的融合蛋白至少保留了天然蛋白的部分抗原性

Glycoprotein I(gI) gene was amplified from genomic DNA of MDV 648 with the dismission of 165bps at the N-terminal of ORF by polymerase chain reaction (PCR) technique. PCR product was cloned into the expressing plasmid vector pGEX_6p_1 via restriction endonucleases BamHI and SmaI. The recombinant was verified by restriction endonuclease analysis and nucleotide sequencing. Then it was transformed into its host E.coli strain BL 21 for gI expression. The expression of gI gene was indentified by SDS_PAGE and...

Glycoprotein I(gI) gene was amplified from genomic DNA of MDV 648 with the dismission of 165bps at the N-terminal of ORF by polymerase chain reaction (PCR) technique. PCR product was cloned into the expressing plasmid vector pGEX_6p_1 via restriction endonucleases BamHI and SmaI. The recombinant was verified by restriction endonuclease analysis and nucleotide sequencing. Then it was transformed into its host E.coli strain BL 21 for gI expression. The expression of gI gene was indentified by SDS_PAGE and Westernblotting, and found to be 63kD in size as a fusion protein with glutathione stransferase(GST) protein. The specific bands of expression was excised from the gel and injected into the mice once a week and for 5 weeks. The antisera was collected from the immunized mice and used for fluorescence assay(FA) with CEF monolayers infected by RB1B and GA of MDV. The positive stains were found in the MDV plaques and localized on the cytomembrane of the infected cells. The results of the study showed that the vitro expressed protein of gI gene via recombinant plasmid vector maintains some antigenicity of MDV.

根据马立克氏病病毒 (MDV)强毒株GA的基因序列 ,设计和合成一对引物 ,以特超强毒 6 48株基因组DNA为模板 ,通过PCR技术 ,扩增其囊膜糖蛋白gI基因阅读框 (ORF)中 ,除去其N_端编码疏水区的 16 5个碱基对 (bp)以外的其余部分 ;将PCR产物按正确的阅读框架定向克隆到表性载体pGEX_6P_1中谷胱甘肽转移酶 (GST)基因的下游 ;将重组质粒转化进大肠杆菌BL2 1株 ,在 1.0mMIPTG浓度和 30℃的条件下诱导 ,gI_GST基因融合蛋白获得了理想的表达 ;经聚丙烯酰胺凝脉电泳 ,West ern_blot试验 ,验证其表达的融合蛋白产物大小为预期的 6 3kD。将表达产物回收后免疫小鼠 ,所得抗血清可与MDV感染的鸡胚成纤维细胞 (CEF)在免疫荧光试验 (FA)中呈细胞膜阳性染色。试验结果表明 ,在大肠杆菌中表达的 6 48株MDVgI基因的融合蛋白产物保留了天然蛋白的某些抗原性

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关糖蛋白gi的内容
在知识搜索中查有关糖蛋白gi的内容
在数字搜索中查有关糖蛋白gi的内容
在概念知识元中查有关糖蛋白gi的内容
在学术趋势中查有关糖蛋白gi的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社