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胃蛋白酶切
相关语句
  pepsin digestion
     Peptide Mapping Analysis of Recombinant Human Neu Epitope Peptide 12 by Pepsin Digestion
     重组人纽表位肽12的胃蛋白酶切肽图分析方法的研究
短句来源
  “胃蛋白酶切”译为未确定词的双语例句
     Fab’ was obtained with a purity of over 90%. 0.6 1.0 mg Fab’(<1.0 ml) was mixed with 0.4 0.8 mg glucoheptonate, followed by adding 5 10 μl freshly prepared SnCl 2 solution (1.0 mg SnCl 2 in 0.01 mol·L -1 HCl).
     方法 :经胃蛋白酶切得的C5 0片断F(ab’) 2 用适量的 2 巯基乙醇还原 ,SephadexG5 0柱分离获得纯度大于 90 %的Fab’片断。
短句来源
     Objective To explore a new way of preventing and curing endotoximia, research about the F(ab′)_2 in the chicken egg yolk antibody against lipopolysaccharide.
     目的研究抗内毒素鸡蛋黄免疫球蛋白(IgY)用胃蛋白酶切后提取的F(ab′)2片段,探索防治内毒素血症的新途径。
短句来源
     Methods Leghorn hens, 25 weeks old, were immunized with lipopolysaccharide . By a simple method of water dilution protein purification, IgY was separated from egg yolk. By the digestion of the pepsin, the F(ab′)_2 were abstracted.
     方法用内毒素(LPS)作抗原免疫25周龄leghorn鸡,改良水溶法提取抗内毒素IgY,胃蛋白酶切后提取IgY F(ab′)2段,光密度法测抗内毒素IgY F(ab′)2浓度和含量、ELISA检测抗内毒素IgY F(ab′)2效价,SDS-聚丙烯酰胺凝胶电泳检测其分子量及纯度。
短句来源
     [Objective] Research about the characteristic of the F(ab)2 in the chicken egg yolk antibody against lipopolysaccharide.
     目的研究抗内毒素IgY用胃蛋白酶切后提取的F(ab)2片段,对其理化特性进行研究。
短句来源
     [Methods] By the digestion of the pepsin, the F(ab)2 were abstracted from IgY against lipopolysaccharide. It was detected by spectrophotometer and analyzed by SDS-PAGE and Western-blotting, and its immunologic competence was determined by enzyme labeling staining reaction. (ELISA).
     方法将抗内毒素IgY用胃蛋白酶切后提取IgY-F(ab)2段,光密度法测抗内毒素IgY-F(ab)2浓度和含量、ELISA检测抗内毒素IgY-F(ab)2效价,SDS-聚内烯酰胺凝胶电泳检测其分子量及纯度,免疫印迹法(Western-blotting)测定其特异性。
短句来源
  相似匹配句对
     Construction of a Plane Tangent to a Quadratic Surface
     二次曲面的平面
短句来源
     THE EXPERIENCE OF EYEBROW ANAPLASTY
     眉体会
短句来源
     Peptide Mapping Analysis of Recombinant Human Neu Epitope Peptide 12 by Pepsin Digestion
     重组人纽表位肽12的胃蛋白酶肽图分析方法的研究
短句来源
     STUDY ON THE CONDITION OF THE IMMOBILIZING OF PEPSIN
     胃蛋白酶固定化条件的研究
短句来源
     STUDY ON THE HYDROLYSIS OF GLOBIN BY PEPSIN
     胃蛋白酶水解珠蛋白的研究
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  pepsin digestion
Left ventricular collagen of various mammals (cat, cow, dog, pig, and rat) was successively extracted with neutral salt, and dilute acid solutions, and limited pepsin digestion.
      
The iliotibial band of the 20-year-old subject contained more collagen that was soluble by a combination of salt, sodium citrate extractions and pepsin digestion.
      
F(ab')2 fragments made by pepsin digestion of the IgG were immunopurified, labelled with biotin and retained activity in the biochemical and histological assays.
      
Collagen was extracted withacetic acid and pepsin digestion, and the final insoluble collagen product was acid-hydrolyzed with 6 N HCL for 24 h at 110°C.
      
Type V collagen was isolated from the human placenta by pepsin digestion and was purified with fractioning salt precipitations.
      
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Objective:To study 99m Tc labeled anti CEA antibody C50 fragment Fab’ and its biodistribution. Methods: F(ab)’ 2 of C50 was reduced by 2 mercapthonal and purified by Sephadex G50 column. Fab’ was obtained with a purity of over 90%. 0.6 1.0 mg Fab’(<1.0 ml) was mixed with 0.4 0.8 mg glucoheptonate, followed by adding 5 10 μl freshly prepared SnCl 2 solution (1.0 mg SnCl 2 in 0.01 mol·L -1 HCl). Then about 1.0 ml of freshly eluted Na 99m TcO 4 was added to the mixture mentioned above...

Objective:To study 99m Tc labeled anti CEA antibody C50 fragment Fab’ and its biodistribution. Methods: F(ab)’ 2 of C50 was reduced by 2 mercapthonal and purified by Sephadex G50 column. Fab’ was obtained with a purity of over 90%. 0.6 1.0 mg Fab’(<1.0 ml) was mixed with 0.4 0.8 mg glucoheptonate, followed by adding 5 10 μl freshly prepared SnCl 2 solution (1.0 mg SnCl 2 in 0.01 mol·L -1 HCl). Then about 1.0 ml of freshly eluted Na 99m TcO 4 was added to the mixture mentioned above and the mixture was slightly shaken. The solution was incubated at room temperature for 15 min. The labeling efficiency was determined by HPLC or ITLC SG method.Nude mice bearing CL 187 tumor xenografts were injected with 99m Tc Fab’ via vein tail and the ID(%)/g (% injected doses /g) of organs were obtained at various time points of postinjection. Results: The labeling efficiency of 99m Tc Fab’ was more than 90%. The ratios of tumor to blood, liver and lung were 1.93, 2.35 and 3.13 4 hours after injection. In addition, these ratios reached up to 4.91, 2.62, and 5.26, 24 hours after injection respectively. 4 hours and 24 hours after injection of 99m Tc Fab’, the ID(%)/g of tumor was 4.53 and 2.73 respectively. Although at the 24 th hour, the ID(%)/g of tumor of 99m Tc C50(intact) reached up to 12.5, the ratio of tumor/blood was only about 1.5. Conclusion: 99m Tc labeled Fab' was successfully prepared by direct labeling technique, and the T/NT ratios were more than 2.0 except that with the kidney which was significantly higher than that of 99m Tc labeled C50 (intact). These are favorable to get clear images at earlier time after injection of 99m Tc Fab'. These indicate that 99m Tc C50 Fab' seems to be a good radiopharmaceutical for RII.

目的 :研究克服99mTc标记抗癌胚抗原 (carcinoembryonicantigen ,CEA)单抗体内生物半衰期长、血液廓清速度慢、晚时相放射性信号弱等缺点的方法 ,用直接标记法进行了99mTc标记抗CEA抗体C5 0片断Fab’的研究。方法 :经胃蛋白酶切得的C5 0片断F(ab’) 2 用适量的 2 巯基乙醇还原 ,SephadexG5 0柱分离获得纯度大于 90 %的Fab’片断。取 0 .6~ 1.0mg纯化的片段Fab’(体积 <1.0ml) ,加入 0 .4~ 0 .8mg葡庚糖酸钠及新鲜配制的SnCl2溶液 5~ 10 μg ,然后加入新鲜淋洗的Na99mTcO4 淋洗液 ,反应 10~ 15min。用高压液相色谱监测放化纯度和抗体纯度。荷CL 187(结肠癌 )裸鼠静脉注射99mTc Fab’ ,观察标记抗体片段在不同时相的体内分布。结果 :标记率大于 90 %。荷瘤裸鼠体内分布结果显示 :在注射99mTc Fab’ 4h后瘤 /血、瘤 /肝、瘤 /肺放射性摄取比分别为 1.93、2 .35和 3 .13 ,2 4h后则分别为 4.91、2 .6 2和 5 .2 6 ,肿...

目的 :研究克服99mTc标记抗癌胚抗原 (carcinoembryonicantigen ,CEA)单抗体内生物半衰期长、血液廓清速度慢、晚时相放射性信号弱等缺点的方法 ,用直接标记法进行了99mTc标记抗CEA抗体C5 0片断Fab’的研究。方法 :经胃蛋白酶切得的C5 0片断F(ab’) 2 用适量的 2 巯基乙醇还原 ,SephadexG5 0柱分离获得纯度大于 90 %的Fab’片断。取 0 .6~ 1.0mg纯化的片段Fab’(体积 <1.0ml) ,加入 0 .4~ 0 .8mg葡庚糖酸钠及新鲜配制的SnCl2溶液 5~ 10 μg ,然后加入新鲜淋洗的Na99mTcO4 淋洗液 ,反应 10~ 15min。用高压液相色谱监测放化纯度和抗体纯度。荷CL 187(结肠癌 )裸鼠静脉注射99mTc Fab’ ,观察标记抗体片段在不同时相的体内分布。结果 :标记率大于 90 %。荷瘤裸鼠体内分布结果显示 :在注射99mTc Fab’ 4h后瘤 /血、瘤 /肝、瘤 /肺放射性摄取比分别为 1.93、2 .35和 3 .13 ,2 4h后则分别为 4.91、2 .6 2和 5 .2 6 ,肿瘤的ID(% ) / g(每克组织的摄取率 )为 2 .73。虽然注射99mTc标记的全抗C5 0后 2 4h ,肿瘤的ID(% ) /g高达 12 .5 ,但瘤 /血比值仅为 1.5 1。结论 :采用99mTc直接法标记Fab’是成功的 ,肿瘤与非肿瘤 (T/NT)的比值除肾外在早期 4h时均大于 2 .0 ,明显高于全抗的T/NT比值 ,有利于在注射后短时

Objective To explore a new way of preventing and curing endotoximia, research about the F(ab′)_2 in the chicken egg yolk antibody against lipopolysaccharide. Methods Leghorn hens, 25 weeks old, were immunized with lipopolysaccharide . By a simple method of water dilution protein purification, IgY was separated from egg yolk. By the digestion of the pepsin, the F(ab′)_2 were abstracted. It was detected by spectrophotometer and analyzed by SDS-PAGE, and its immunologic competence was determined by enzyme labeling...

Objective To explore a new way of preventing and curing endotoximia, research about the F(ab′)_2 in the chicken egg yolk antibody against lipopolysaccharide. Methods Leghorn hens, 25 weeks old, were immunized with lipopolysaccharide . By a simple method of water dilution protein purification, IgY was separated from egg yolk. By the digestion of the pepsin, the F(ab′)_2 were abstracted. It was detected by spectrophotometer and analyzed by SDS-PAGE, and its immunologic competence was determined by enzyme labeling staining reaction (ELISA) . Results IgY-F(ab′)_2 from lipopolysaccharide was found 1.04 mg/ml-egg yolk and 90% purity. Its molecular weight was 24 000. The immunochemistry were 1∶25 600. Conclusion The IgY-F(ab′)_2 against lipopolysaccharide has high titer, well specificity and large production.

目的研究抗内毒素鸡蛋黄免疫球蛋白(IgY)用胃蛋白酶切后提取的F(ab′)2片段,探索防治内毒素血症的新途径。方法用内毒素(LPS)作抗原免疫25周龄leghorn鸡,改良水溶法提取抗内毒素IgY,胃蛋白酶切后提取IgY F(ab′)2段,光密度法测抗内毒素IgY F(ab′)2浓度和含量、ELISA检测抗内毒素IgY F(ab′)2效价,SDS-聚丙烯酰胺凝胶电泳检测其分子量及纯度。结果抗内毒素IgY F(ab′)2含量为1.04mg/ml蛋黄液,效价为1∶25600,纯度为90%,相对分子质量为24000。结论抗内毒素IgY F(ab′)2产量大、效价高、特异性强。

[Objective] Research about the characteristic of the F(ab)2 in the chicken egg yolk antibody against lipopolysaccharide. [Methods] By the digestion of the pepsin, the F(ab)2 were abstracted from IgY against lipopolysaccharide. It was detected by spectrophotometer and analyzed by SDS-PAGE and Western-blotting, and its immunologic competence was determined by enzyme labeling staining reaction. (ELISA). [Results] IgY-F(ab)2 from lipopolysaccharide was found 1.04 mg/mL-egg yolk and 90% purity. Its molecular weight...

[Objective] Research about the characteristic of the F(ab)2 in the chicken egg yolk antibody against lipopolysaccharide. [Methods] By the digestion of the pepsin, the F(ab)2 were abstracted from IgY against lipopolysaccharide. It was detected by spectrophotometer and analyzed by SDS-PAGE and Western-blotting, and its immunologic competence was determined by enzyme labeling staining reaction. (ELISA). [Results] IgY-F(ab)2 from lipopolysaccharide was found 1.04 mg/mL-egg yolk and 90% purity. Its molecular weight was 24 KD. The immunochemistry were 1∶25600, the IgY-F(ab)2 has the same immunity characteristic with the IgG of chicken serum. [Conclusion] The IgY-F(ab)2 against lipopolysaccharide has high titer, well specificity and large production.

目的研究抗内毒素IgY用胃蛋白酶切后提取的F(ab)2片段,对其理化特性进行研究。方法将抗内毒素IgY用胃蛋白酶切后提取IgY-F(ab)2段,光密度法测抗内毒素IgY-F(ab)2浓度和含量、ELISA检测抗内毒素IgY-F(ab)2效价,SDS-聚内烯酰胺凝胶电泳检测其分子量及纯度,免疫印迹法(Western-blotting)测定其特异性。结果抗内毒素IgY-F(ab)2含量为1.04mg/mL蛋黄液,效价为1∶25600,纯度为90%,分子量为24KD,与鸡血清中的IgG有相同的抗原性。结论抗内毒素IgY-F(ab)2产量大、效价高、特异性强。

 
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