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再生培养基
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  regeneration medium
     and the optimal regeneration medium for tube chip was MS+ZT 2mg/L+IAA 1mg/L.
     薯片再生培养基为MS+ZT 2mg/L+IAA 1mg/L。
短句来源
     And the oplimal regeneration medium for tuber disc was MS+ 3.5mg/L ZT + 0.88mg/L IAA.
     薯块再生培养基确定为MS+ZT 3.5 mg/L+IAA 0.88mg/L。
短句来源
     The regeneration medium is: MS + NAA 0.2mg/L-6-BA 2.Om1L and the medium of MS + GA3 5nig/L + 6-BA 2.25nig/L was usedafter two weeks.
     以M_1培养基作为植株再生培养基,即MS+NAA 0.2mg/L+6-BA 2.0mg/L,2-3周后转至MS+GA_35mg/L+6-BA 2.25mg/L;
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     The transformants on the regeneration medium containing neomycin (10 μg/ml), penicillin (25μg/ml) and tetracycline (12.5μg/ml) are picked up.
     在含新霉素(10μg/ml)、青霉素(25μg/ml)、四环素(12.5μg/ml)的高渗蔗糖再生培养基上分别获得了转化子。
短句来源
     The best shoot elongation medium is MS supplemented with 0.15mg/L 6-BA, 0.1mg/L NAA, 0.1mg/L KT, 20mg/L Ad; the best leaf regeneration medium is MS supplemented with 0.5mg/L 6-BA, 0.1mg/L NAA;
     通过不同激素浓度配比的实验,确定了最佳壮苗培养基(MS + 0.15mg/L 6-BA + 0.1mg/L NAA + 0.1mg/L KT + 20mg/L Ad)叶片再生培养基(MS + 0.5mg/L 6-BA + 0.1mg/L NAA)?
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  regeneration media
     The best regeneration media were as follows:MS + 0.8mg/L BA + 0.08mg/L NAA for P.×euramricana (Dode) Guineir cv. ‘zhonglin-46’;
     再生培养基为:中林46杨:MS + 0.8mg/L BA + 0.08mg/L NAA;
短句来源
     The optimum regeneration media for Ponkan explants cultured horizontally and vertically were MT plus 1.0 mg/L of BA and 0.5 mg/L of IAA and MT plus 1.0 mg/L of BA and 1.0 mg/L of IAA respectively.
     椪柑上胚轴水平和垂直放置时最适宜的再生培养基分别为:MT+1.0mg/L BA+0.5mg/L IAA和MT+1.0mg/LBA+1.0mg/L IAA; 锦橙上胚轴水平放置时最适宜的再生培养基为:MT+1.0mg/LBA+0.5mg/L IAA。
短句来源
     The compound enzyme was composed of snailase , celluase, lysozyme at the best concentration ratio of 5∶4∶1, pH value 5.0. The OS double layer regeneration media was the best media for L 1 protoplast regeneration.
     用 0 .4mol/L NH4Cl,1 0 mmol/L Mg SO4作渗透压稳定剂时 ,原生质体数量达到 4.32× 1 0 5个 /mg。 OS双层再生培养基最适于原生质体再生
短句来源
     The effects of such factors as mycelial age,osmotic stabilizers,enzymes and their concentration,lysis time and regeneration media on the formation and regeneration of the protoplasts of Paecilomyces lilacinus were studied. The optimum conditions are as follows: mycelia of 20h,5mg/mL snailase,0.8mol/L NaCl as osmotic stabilizer and lysis time of 2~3h.
     研究了菌龄、渗透压稳定剂、酶的种类及浓度、酶解时间和再生培养基等因素对淡紫拟青霉菌IPC菌株原生质体形成和再生的影响,结果表明,IPC菌株原生质体形成的最适宜条件为:菌龄20 h,用5 mg/mL蜗牛酶,0.8 mol/L NaCl的渗透压稳定剂,酶解2~3 h;
短句来源
     With petiole of as explants, the shoots were derived from loose callus or through organogenesis, and 57.1% regeneration rate was reached following 2-stage procedure: 1) initial dark induction for 10 days in the media of MS plus 7uM thidiazuron (TDZ); 2) the explants were transferred to regeneration media of MS plus 0.5mg/l 6-BA for 20 days.
     以叶柄为外植体和MS为基础培养基,在添加7μM TDZ的诱导培养基中暗培养10天后,转接到添加0.5mg/l 6-BA的再生培养基中光周期培养约20天,可以获得57.1%的芽再生率,为最适再生培养条件。
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  “再生培养基”译为未确定词的双语例句
     The result showed that the most suitable medium of inducing and proliferating callus is MS+6-BA 2.0 mg/L +NAA 2.0 mg/L,medium of regenerating adventitious bud is MS+6-BA 2.0 mg/L+NAA0.2 mg/L,rooting medium is 1/2MS+IAB3.0 mg/L+0.1% mg/L active carbon.
     研究结果表明:愈伤组织诱导和增殖培养基为MS+6-BA2.0 mg/L+NAA2.0 mg/L,不定芽再生培养基为MS+6-BA2.0 mg/L+NAA0.2 mg/L,生根培养基为1/2MS+IAB3.0 mg/L+0.1%活性炭是较佳的.
短句来源
     The differentiation medium was MS supplemented with 0.5mg/L 6-BA and 1.0mg/L KT.
     再生培养基为附加6-BA 0.5mg/L、KT 1.0mg/L的MS培养基。
短句来源
     2 types of hormone combinations (NAA 0.50 mg·L -1+BA 10.0 mg·L -1, NAA 0.05 mg·L -1+TDZ 3.0 mg·L -1) were favorable to shoots differentiation for the immature cotyledons.
     幼胚子叶不定芽再生培养基的激素配比为NAA0.50mg·L-1+BA10.0mg·L-1、NAA0.05mg·L-1+TDZ3.0mg·L-1;
短句来源
     ④The suitable concentration of 6-BA and KT in medium for plantlet regeneration were 0.05 mg/L and 0.2 mg/L.
     4)愈伤组织再生培养基中6-BA的适宜浓度为0.05 mg/L,KT的适宜浓度为0.2 mg/L。
短句来源
     The medium with the following composition: potato 200g,sucrose 20g,peptone 2g,yeast extracts 2g,KH_2PO_4 1.5g,K_2HPO_4 1.5g,MgSO_4·7H_2O 1.5g,vitamin B_1 0.1g,vitamin B_6 0.1g,0.6mol/L mannitol was suitable for protoplast regeneration.
     原生质体适宜再生培养基为马铃薯200g,蔗糖20g,蛋白胨2g,酵母粉2g,KH2PO41.5g,K2HPO41.5g,MgSO4.7H2O 1.5g,维生素B10.1g,维生素B60.1g,0.6mol/L甘露醇。
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  regeneration medium
The regeneration medium should be complemented by hormones, 2.5 mg/l auxin 2,4-D and 0.1-0.5 mg/l cytokinin 6-BAP, to increase the frequency of somatic embryo maturation.
      
The yield of plants from one explant was doubled due to the use of maltose in the regeneration medium.
      
The addition of ABA to the regeneration medium usually reduced shoot regeneration frequency; however, the inhibitory effect of ABA depended on other growth regulators.
      
Organogenic callus was subsequently transferred to shoot regeneration medium.
      
Spontaneous regeneration of roots on shoots was evidenced on regeneration medium itself.
      
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  regeneration media
Green somatic hybrid calluses were visible against a background of albino cells/calluses, and upon transfer to regeneration media gave rise to shoots.
      
Progeny were evaluated in vitro on two regeneration media for callus growth and differentiation.
      
Highly significant additive genetic correlation of performance on two regeneration media was found.
      
No significant differences for embryogenesis were attributable to differences in the regeneration media used.
      
Furthermore, no interaction of additive genetic effects with regeneration media were observed.
      
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The protoplast of Bacillus subtilis BD366(pUB110) (Thr- Try- Azr Kmr Smr) and B.subtilis Ki-2-132 (Thr- Val- Ile- Kmn Smr) were fused by polyethene glycol (PEG) in the presence of deoxyribonuelease.The colonies of fusion cells resistant to streptomycin and kanamycin were obtained from regenerants on regeneration medium at a frequency ranging from 10-4 to 10-7 per regeneration colony.The genetic markers for resistance streptomycin and resistance kanamycin of fusion cell were stable throughout the text.The fusion...

The protoplast of Bacillus subtilis BD366(pUB110) (Thr- Try- Azr Kmr Smr) and B.subtilis Ki-2-132 (Thr- Val- Ile- Kmn Smr) were fused by polyethene glycol (PEG) in the presence of deoxyribonuelease.The colonies of fusion cells resistant to streptomycin and kanamycin were obtained from regenerants on regeneration medium at a frequency ranging from 10-4 to 10-7 per regeneration colony.The genetic markers for resistance streptomycin and resistance kanamycin of fusion cell were stable throughout the text.The fusion cell colonies with a shape like the colonies of B.subtilis Ki-2-132,were selected from regenerants.It was shown by transformation that plasmid DNA isolated from fusion cells carried Kmr gene.The transformation frequency of plasmid DNA of fusion cells was 10-5-10-8.The transformation efficiency of plasmid DNA of fusion cell was the same as that of pUB110 DNA under the same condition.The electrophoretic behavior of plasmid DNA isolated from fusion cells was the same as that of pUB110 DNA.Therefore,the transfer of plasmid pUB110 by the protoplasts fusion was demonstrated.

在脱氧核糖核酸酶(DNase)存在的情况下,用聚乙二醇(PEG)方法,将枯草杆菌BD366(pUB110)(Thr~- Try~- Az~- Km~r Sm~s)的原生质体与枯草杆菌Ki-2-132(Thr~- Val~- Ile~- Km~sSm~r)的原生质体进行融合,在再生培养基上再生细胞壁,获得同时抗Km和Sm的融合体。融合频率在10~(-4)—10~(-7)之间。经测定,融合体的遗传性稳定。从融合体中挑出菌落形态与Ki-2-132相同的融合体A18、B20和B7,其质粒DNA带有Km~r基因,而且其琼脂糖凝胶电泳图与pUB110质粒DNA相同。因而确认pUB110质粒通过原生质体融合而进行转移。

This article deals with the conditions of formation, regeneration and fused recombination by protoplasts of four auxo-trophic strains of St. mycarofaciens var si-chuanensis n.var 113, as well as the metbod of isolating stable fused recombinants.The protoplasts of St. mycarofaciens were formed by a modification of the gly-cine-lysozyme method.The cell wall of my-celia grown in the S-medium containing 0.5 ~1.0% glycine was sensitive to lysozyme, but it wasn't partially growth-inhibitory concentration of glycine....

This article deals with the conditions of formation, regeneration and fused recombination by protoplasts of four auxo-trophic strains of St. mycarofaciens var si-chuanensis n.var 113, as well as the metbod of isolating stable fused recombinants.The protoplasts of St. mycarofaciens were formed by a modification of the gly-cine-lysozyme method.The cell wall of my-celia grown in the S-medium containing 0.5 ~1.0% glycine was sensitive to lysozyme, but it wasn't partially growth-inhibitory concentration of glycine. The regenerated rate of protoplasts on complete medium (MgSO4 doubling) 5.8~52% was higher than R2-medium(0~10.3%). The fusion of protoplasts were effective with PEG 4000 and 6000, 30% concentration (w/v) with 3.68%CaCl2 solution allocation,treated 30 minutes. Electronic microscopic observation showed the procedure of protoplasts regeneration.The stable genetic recombinants were obtained by indirect selection method. The fused recombination frequency was 10-2 to 10-3.A11 results showed a marked difference on morphologic and genetic characters between the recombinants and parents, and between the different recombinants.The antibiotic producing ability of the stable fused recombinant C69 was 40% higher than its parents strains. The strain C39-112 obtained by UV treatment was successfully isolated and its antibiotic yields were 150% higher than its parent strains. Astrain C1 without any antibiotic producing ability was also obtained.

用甘氨酸—溶菌酶方法成功地分离生米卡链要菌(S.mycarofaciens)原生质体。在MgSO_4加倍的完全培养基(cm)上,原生质体再生频率远远超过R_2再生培养基。用3.68%的CaCl_2溶液,配制成30%(w/v)的PEG4000或6000溶液,可有效地诱导生米卡链霉菌原生质体融合,融合重组频率10~(-2)~10~(-3)。融合重组体和亲本在菌落形态、孢子形态和抗生素产量上有显著差异,不同的融合重组体之间,也表现了这些差异。实验分离到抗生素生产能力超过亲本40%的C_(69)菌株,经紫外光诱变后,又获得抗生素能力超过亲本15%(均以两亲本的平均值计)以上的C_(64112)菌株。融合重组体对紫外光的敏感度低于亲本,而抗生素生产能力的正变频率超过亲本。

High Concentration Protoplast Suspensi-on(10~5- 10~7/ml) was prepared from Penicilli-um chrysogenum mycelia by 0.55 MNaCl os-motically-stabilized solution of cellulase. Regeneration frequency of purified protoplast suspension plated on osmotical RM-medium was about 40%.

产黄青霉菌球状菌株原生质体的融合是用两株不同遗传标记的菌株作亲本,用玻璃纸平板培养菌丝体,在高渗透性稳定剂中用纤维素酶裂解菌丝细胞壁,制备高浓度(10~6~10~7/ml)原生质体悬浮液。 将原生质体悬浮液接种到高渗性再生培养基上,再生频率可达40%左右。作渗透处理后,测得菌丝断片含量在0.01~0.1%之间。 两菌株的原生质体以1∶1相混合,用分子量6000,30%聚乙二醇处理后,发生了原生质体间的凝聚。将混合物涂布在高渗性合成培养基上,产生了异核体菌落,频率为0.40%左右。将少量异核体分生孢子分离在菌丝生长培养基上后,产生了数量相仿的两亲本型分离子。将大量异核体产生的分生孢子接种到合成培养基上后,能得到少数原养型杂合二倍体,频率为0.035%左右,杂合二倍体纯化后,再以紫外线诱发,获得了以角变和斑点形式出现的二亲本型分离子。

 
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