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原生质体释放
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  frequency of protoplast
     Under the optimum condition, the concentration and regeneration frequency of protoplast were 1.6×10 9/ml and 29.5% respectively.
     在最适条件下原生质体释放浓度和再生率分别为1.6×109/ml和29.5%。
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  “原生质体释放”译为未确定词的双语例句
     Among five osmotic stabilizers tested,MgSO 40.6mol/L and mannitol 0.6mol/L gave higher protoplasts yield.
     渗透压稳定剂以MgSO40 .6mol/L及甘露醇 0 .6mol/L处理原生质体释放量最高。
短句来源
     Mycelia of Curvularia lunata cultured for 22h were harvested and suspended in 0.3mol/L DTT for 20min. The protoplasts yield achieved 8.15×10 6 /ml under 30℃ with 0.5% novozym and 1% lywallzyme(pH5.6) using 0.6mol/L KCl as osmotic stabilizer in 3.5h.
     将培养 2 2h的菌丝体用0 .3mol L的二硫苏糖醇处理 2 0min ,0 .6mol L的KCl作为渗稳剂 ,30℃下经 0 .5 %新生酶 2 34和 1%溶壁酶混合液(pH5 .6 )酶解 3.5h ,原生质体释放量达到最大值 8.15× 10 6 个 ml,再生率为 2 3.0 7%。
短句来源
     The results showed that co-function protoplast of the 2% Lywallzyme plus 2% Snailase was the most effective way to its yield, 1.25 × 106 for CL and 1.21 × 106 for EM, respectively.
     结果表明,2%溶壁酶加2%蜗牛酶处理获得的两亲株的原生质体释放数量最高, CL菌和EM菌分别达到1.25×106个/ml和1.21×106个/ml;
     0.6mol/L KG1 as the osmotic stabilizers, the protoplasts yield was 16.11 x 106/ml from the mycelia with 16~18 hours-old age which were digested in 1% lywallzyme and 1% celluase at 30C for 4-5 hours.
     首先确定了新月弯抱霉原生质体的形成条件,将培养 16 h~18 h的菌丝体,以 0石mol/L KCI作为渗透压稳定剂,30C下,经过 1%溶壁酶和 1%纤维素酶混合酶液…H 5石)消化 4 h~5 h,原生质体释放量达到最高,为 6.11XI0~mL。
短句来源
     Stable protoplast release is obtained when pH value of enzyme solution is in the range of 5.0 to 7.0. Among four osmotic stabilization tested, citric-phosphoric acid buffer (pH 6.0, containing 0.8?mol/L sorbitol) gives the highest protoplasts yield.
     酶解液 pH值在 5 .0~ 7.0时原生质体的产量较高 ,渗透压稳定剂以柠檬酸 -磷酸缓冲液( pH6.0 ,内含 0 .8mol/L山梨糖醇 )处理原生质体释放量最高。
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  相似匹配句对
     Study on Liberation and Reversion of Protoplastof Auricularia auricula
     木耳原生质体释放和再生研究
短句来源
     STUDIES OF THE PROTOPLAST RELEASE AND REGENERATION IN THE GENUS PLEUROTUS (Ⅰ)
     侧耳属原生质体释放与再生(Ⅰ)
短句来源
     ENERGY'S RELEASING
     释放活力
短句来源
     releasing pattern;
     释放形式;
短句来源
     Cryopreservation of Citrus Protoplasts
     柑橘原生质体的超低温保存
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  protoplast liberation
Effects of osmotic stabilizers on the activities of mycolytic enzymes used in fungal protoplast liberation
      
Assay methods for chitinase, β-glucanase and α-glucanase in the presence of the osmotic stabilizers used in fungal protoplast liberation were developed.
      
MgSO4, KCl and NH4Cl were equally efficient as stabilizers for protoplast liberation, although they had different effects on chitinase activity.
      
As chitin is the major component of the fungal cell wall, chitinase is thought to be more important for protoplast liberation than are β-glucanase and α-glucanase.
      
  frequency of protoplast
There is a correlation between the distribution of nuclei in hyphal fragments and protoplasts and the frequency of protoplast regeneration.
      
The shoot regeneration frequency of protoplast-derived calli was in the order of 60%.
      
The frequency of protoplast cell divisions and colony formations in liquid culture medium were similar to those on agarose-solidified medium.
      
The influence of the exogenous polyamines: putrescine, spermidine and spermine, on the frequency of protoplast divisions for 2 genotypes of sugar beet (Beta vulgaris L.) was analyzed.
      
The frequencies of calluses forming plants ranged from 6.0% to 41.3% based on the different concentrations of IAA and BAP, and 2.0?mg?l-1 BAP gave the highest regeneration frequency of protoplast-derived calluses in I.
      
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High yields of protoplasts from two Penicillium auxotrophs were obtained by using a combined enzyme system containing cellulase from Trichoderma pseudokoningii plus extract of Achatina fulica Ferussac digestive juice.The effects of factors such as osmotic stabilizers, temperature, pH, modes of incubation and cultivation media on the formation and regeneration of protoplasts in these auxotrophs were studied.The protoplasts of P. chrysogenum 801 were released rapidly, and were relatively small and unstable.

采用0.5%纤维素酶(来自拟康氏木霉Trichoderma PseudokGningii)加0.5%褐云玛瑙螺酶(Achatina fulica Ferussac的消化液)的混合酶,自产黄青霉的两个营养缺陷型中获得了大量的原生质体。比较了渗透压稳定剂、温度、pH值、酶解温育方式和菌丝体的培养基成份等因素对两菌株原生质体的形成和再生的作用。801菌株的原生质体直径较小,原生质体的释放早而不稳定,但对钠离子较适宜,并且在后期有钙离子时较为稳定。822菌株的原生质体直径较大,释放较迟,较稳定,对钾钠两种离子的影响差异并不大。

The present report deals with the

本工作研究了豆科植物紫云英的叶片及叶肉原生质体的培养。叶片培养实验表明,诱导愈伤组织的最适培养基为MS加1.0-2.0毫克/升2,4-D和0.25毫克/升KT;诱导根分化需加1.0—5.0毫克/升NAA和0.5毫克/升BA;而苗分化则以0—0.5毫克/升IAA和0.5毫克/升BA为好。高浓度的NAA有利于根分化而抑制茎芽形成;高浓度的IAA对根和芽分化都有抑制作用。叶肉原生质体分离和培养试验表明,紫云英叶肉原生质体的释放及其培养活力受叶龄、植株生理状态和酶浓度的影响。叶肉原生质体在改良的KM8P培养基中能分裂。用改良KM8细胞培养基定期稀释,可使分裂持续进行而得到细胞团。BA和2,4-D为诱导紫云英叶肉原生质体分裂所必需。其最佳组合激素为BA 0.21毫克/升和2,4-D 1.13毫克/升。葡萄糖作为渗透压稳定剂时,其浓度明显影响原生质体的存活率。弱光条件下培养比黑暗培养有利于叶肉原生质体分裂。由叶肉原生质体形成的愈伤组织能形成瘤状结构和根。

High yields of protoplast from JN15 and Jul of Penicillium decumbens were readily prepared through enzymatic hydrolysis of non-pretreated mycelial suspensions which were 22-25h culture of JN 15 and 33-36h culture of JU1, utilizing a mixture of 0.04% helicase and 0.5% cellulase at pH 6.5, 35℃, with 0.6M(NH_4)_2SO_4 as osmotic stabilizer. When mycelia at exponential phase of growth were hydrolyzed with various enzymatic concentrations, the yields of protoplast were directly proportional to enzymatic concentrations...

High yields of protoplast from JN15 and Jul of Penicillium decumbens were readily prepared through enzymatic hydrolysis of non-pretreated mycelial suspensions which were 22-25h culture of JN 15 and 33-36h culture of JU1, utilizing a mixture of 0.04% helicase and 0.5% cellulase at pH 6.5, 35℃, with 0.6M(NH_4)_2SO_4 as osmotic stabilizer. When mycelia at exponential phase of growth were hydrolyzed with various enzymatic concentrations, the yields of protoplast were directly proportional to enzymatic concentrations within the range tested; While the frequencies of regeneration to the mycelial form were decreased as the enzymatic concentrations increased. The correlation was discussed between enzymatic concentration and regeneration viability of protoplast in this paper, thus optimal concentrations of enzyme for preparing stable protoplast were showed. The effect of temperature, PH, and concentrations of stabilizer, on the formation and regeneration of protoplast was examined. Additionaly, morphology of the protoplast formation and regeneration to the mycelial form were also observed by phase-contrast microscope.

用脱壁酶(含0.04%的蜗牛酶和0.5%的纤维素酶)以0.6M的(NH_4)_2SO_4作稳定剂,于pH6.5,35℃直接酶解不经任何预处理的培养22~25小时和33~36小时的斜卧青霉JN15、Jul的菌丝体,得到大量具再生能力的原生质体。用不同浓度的脱壁酶处理指数生长期的菌丝体,在本试验范围内,原生质体的形成量随酶浓度的增高而增加,其再生频率随酶浓度的升高而下降;对两者的关系进行了讨论,并给出了获得大量具再生活性的原生质体的脱壁酶的浓度范围。同时,研究了温度,pH、渗透压稳定剂的浓度对原生质体形成和再生的作用;在相差显微镜下,详细观察了原生质体的释放过程、形态变化以及原生质体再生成正常茵丝的全过程。

 
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