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   高压水注射 的翻译结果: 查询用时:0.522秒
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高压水注射
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  “高压水注射”译为未确定词的双语例句
     pUC18-HBV1. 3 containing a replication-competent, HBV1.3 supergenomic DNA, was injected into BALB/cJ mice through tail vain. HBV viremia was measured by real-time PCR. Serum concentrations of HBsAg were detected by sandwich ELISA HBeAg was detected and quantified by RIA using HBcAg standards to generate.
     采用高压水注射方法,通过尾静脉将具有复制能力的HBV质粒导入BABL/cJ小鼠体内,应用real-time PCR、ELISA、RIA、Southern Blot、Northern Blot,以及免疫组化等方法,检测小鼠病毒血症、血清和肝组织中HBV 抗原表达动态变化、肝组织中HBV转录和复制情况,以及小鼠免疫应答状况。
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  相似匹配句对
     High pressure stream of water
     高压射流
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     Survey on the high pressure water jet technology
     高压射流技术综述
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     They always connected water with their attitude.
    
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     Water
    
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     Inoculation of E.
     单独注射E.
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  hydrodynamic injection
The linearity of calibration curve provided by SEKI was similar to that offered by non-bias hydrodynamic injection (HDI) but significantly better than that obtained by EKI.
      
Based on the linear relationship between large sample injection time during hydrodynamic injection and migration time in non-stacking runs, a new method is proposed to measure average hydrodynamic velocity in capillary zone electrophoresis (CZE).
      
The different variables that affect the separation were studied and optimized; the compounds were detected at low mg L-1 levels after hydrodynamic injection under pressure.
      
After electrokinetic or hydrodynamic injection calibration plots of peak area against concentration were linear between 0.05 and 10 μg mL-1.
      
The detection limits for a signalto-noise ratio of 3 and 10s hydrodynamic injection were 5×10-6 mol L-1 for Cu(II) and 1×10-6 mol L-1 for Co(III).
      
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Using hydrodynamic injection. pUC18-HBV1. 3 containing a replication-competent, HBV1.3 supergenomic DNA, was injected into BALB/cJ mice through tail vain. HBV viremia was measured by real-time PCR. Serum concentrations of HBsAg were detected by sandwich ELISA HBeAg was detected and quantified by RIA using HBcAg standards to generate. Antibodies specific for HBsAg, and HBcAg were assayed by endpoint titration ELISA. HBV core protein expressed in the liver was visualized by immunohistochemical staining. Viral...

Using hydrodynamic injection. pUC18-HBV1. 3 containing a replication-competent, HBV1.3 supergenomic DNA, was injected into BALB/cJ mice through tail vain. HBV viremia was measured by real-time PCR. Serum concentrations of HBsAg were detected by sandwich ELISA HBeAg was detected and quantified by RIA using HBcAg standards to generate. Antibodies specific for HBsAg, and HBcAg were assayed by endpoint titration ELISA. HBV core protein expressed in the liver was visualized by immunohistochemical staining. Viral RNA and replicative DNA intermediates in liver were detected by Northern and Southern blot analyses, respectively. Hydrodynamic injection of pUC18-HBV1. 3 lead to viral gene expression and replication in vivo . The immune response to HBV. which was comparable with the characteristic response during acute HBV infection in man, was elicited in mice. A mouse model of acute hepatitis B virus infection can be developed by transfection of hepatocytes in vivo .

采用高压水注射方法,通过尾静脉将具有复制能力的HBV质粒导入BABL/cJ小鼠体内,应用real-time PCR、ELISA、RIA、Southern Blot、Northern Blot,以及免疫组化等方法,检测小鼠病毒血症、血清和肝组织中HBV 抗原表达动态变化、肝组织中HBV转录和复制情况,以及小鼠免疫应答状况。结果HBV基因可以在小鼠体内表 达和复制,并诱导小鼠产生特异性免疫应答,其应答模式及HBV清除过程与人类的HBV急性感染类似。实验显 示高压注射具有复制能力的HBV质粒可以在小鼠体内建立HBV急性感染模型,这种模型可以用于HBV病毒 学、免疫学以及抗病毒药物筛选等方向的研究。

 
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