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   氨基酸进化 的翻译结果: 查询用时:0.291秒
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氨基酸进化
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  “氨基酸进化”译为未确定词的双语例句
     Therefore, this study seeks some computational approaches to predict protein-protein interactions, and focuses on the prediction of protein interactions and interacting sites.
     1) 提出了一种新的基于氨基酸进化保守性的蛋白质相互作用位点预测方法。 由于自然选择法则的作用,在一个蛋白质家族中与分子功能相关的氨基酸残基在进化过程中呈现保守性特征,蛋白质与外界的作用依赖于这些关键的残基。
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     The purpose of this investigation is to compare the structural difference of atpA genes in cytoplasmic male sterile line and its maintainer line of tuber mustard. The intact atpA genes were cloned and determined to be 1524 bp in size,and deduced to code for 507 amino acids. Phylogenetic analysis indicated that it was highly conservative among Crucerfera family.
     以榨菜细胞质雄性不育系及其保持系基因组总DNA为模板,通过特异引物PCR方法克隆了榨菜atpA基因,基因大小为1524 bp,推定编码507个氨基酸,进化分析表明atpA基因非常保守;
  相似匹配句对
     Evolution of Amino Acids & Differentiation among Genetic Code Systems
     氨基酸进化与遗传密码系统的分化
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     On Evolution of Nature Value
     自然价值的进化
短句来源
     Amino Acid Sequence and Molecular Evolution Analysis of Plastocyanin from Ulva pertusa
     孔石莼质体蓝素氨基酸序列分析和分子进化
短句来源
     On the Evolution of the Spider Web
     蛛网的进化
短句来源
     CHEMICAL SYNTHESIS OF AMINO ACIDS
     氨基酸的化学合成
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  evolution of amino acid
Simulation studies on the evolution of amino acid sequences
      
These rules are (1) conservative evolution of amino acid and adapter binding sites and (2) minimization of the disruptive effects on protein structure caused by codon meaning changes.
      
The Evolution of Amino Acid Repeat Arrays in Plasmodium and Other Organisms
      


Objective To clarify the features of gene variation among epidemic strains of human rotavirus NSP4 in China. Methods NSP4 genes from 27 epidemic strains of human rotavirus isolated in different area of China in recent years were amplified with RT PCR, the resulted cDNAs were cloned and sequenced. The sequences of full length cDNAs were compared with 10 rotavirus NSP4 sequences available in the GenBank using the Clustal×1.8,TreeView32 and DNA Star softwares. The G serotype of VP7 was analysised by PCR. Results...

Objective To clarify the features of gene variation among epidemic strains of human rotavirus NSP4 in China. Methods NSP4 genes from 27 epidemic strains of human rotavirus isolated in different area of China in recent years were amplified with RT PCR, the resulted cDNAs were cloned and sequenced. The sequences of full length cDNAs were compared with 10 rotavirus NSP4 sequences available in the GenBank using the Clustal×1.8,TreeView32 and DNA Star softwares. The G serotype of VP7 was analysised by PCR. Results The homology of the amino acid among the 27 rotavirus strains isolated in China was 81.7%~99 4%. Based on the variation of amino acid sequence, the virus strains can be divided into two groups, represented by Wa and KUN with the homology of 92.0%~99.4% and 92.0%~98.9% within each group, respectively. The diversity between the two groups were 16.6%~21.0%. The Wa group could further be separated into three subgroups, according to the diversity between those strains and the characterization in the highly variable domain. The association between VP7 serotype and NSP4 genotype was not strong. Conclusion The NSP4 gene of human rotavirus epidemic strains in China can be divided into Wa and KUN two groups, Wa group is the main group and contains three subgroups possessing characteristic amino acid sites. Samples isolated in the same year but not in the same area shared higher homology.

目的 研究我国轮状病毒流行株NSP4基因的变异特点。方法 对近年来从我国不同地区获得的 2 7份人轮状病毒流行株的NSP4基因用RT PCR进行扩增 ,克隆后进行全长cDNA序列分析 ,并利用Clustal× 1.8,TreeView3 2及DNAStar软件与参比株Wa、KUN、AU 1、EW及来自GenBank的OSU、SA11、Hochi、US2 44、Bristol株的NSP4序列进行分析比较。采用PCR分型方法对VP7血清型进行鉴定 ,确定轮状病毒G型与NSP4基因型的关系。结果 氨基酸同源性比较表明 ,我国轮状病毒不同流行株NSP4之间同源性为 81.7%~ 99.4% ,据此可将 2 7株RVNSP4分为 2组 ,分别以Wa株和KUN株为代表 ,其中以Wa组为主。组内同源性分别为 92 .0 %~ 99.4%和 92 .0 %~ 98.9% ,组内变异率分别为 0~ 8 5 %及 1 2 %~ 8 5 %。两组间变异率达 16 6%~ 2 1 0 %。氨基酸进化树提示在Wa组内包括 3个亚组。轮状病毒G血清型与NSP4基因型之间的联系不确定。结论 我国流行株NSP4基因主要可分为Wa...

目的 研究我国轮状病毒流行株NSP4基因的变异特点。方法 对近年来从我国不同地区获得的 2 7份人轮状病毒流行株的NSP4基因用RT PCR进行扩增 ,克隆后进行全长cDNA序列分析 ,并利用Clustal× 1.8,TreeView3 2及DNAStar软件与参比株Wa、KUN、AU 1、EW及来自GenBank的OSU、SA11、Hochi、US2 44、Bristol株的NSP4序列进行分析比较。采用PCR分型方法对VP7血清型进行鉴定 ,确定轮状病毒G型与NSP4基因型的关系。结果 氨基酸同源性比较表明 ,我国轮状病毒不同流行株NSP4之间同源性为 81.7%~ 99.4% ,据此可将 2 7株RVNSP4分为 2组 ,分别以Wa株和KUN株为代表 ,其中以Wa组为主。组内同源性分别为 92 .0 %~ 99.4%和 92 .0 %~ 98.9% ,组内变异率分别为 0~ 8 5 %及 1 2 %~ 8 5 %。两组间变异率达 16 6%~ 2 1 0 %。氨基酸进化树提示在Wa组内包括 3个亚组。轮状病毒G血清型与NSP4基因型之间的联系不确定。结论 我国流行株NSP4基因主要可分为Wa组和KUN组 ,在Wa组内可形成三个亚组 ,并且在高变区有特征性的氨基酸位点。NSP4的变异与年份有关而与地域关系不密切

A pair of specific primers were desigend and synthesized according to the published sequence of M gene of IBV in GeneBank.cDNA of M gene was obtained by RT-PCR from viral RNA of IBVSAIBWJ strain,and further cloned into PGEM-T vector,and identified the correct plasmid by PCR and double digestion.The M gene was sub-cloned into pcDNA3.1(+)plasmid be-tween HindⅢand XhoⅠsites to construct the IBV M gene expressing plasmid with CVM promoter and BGH polyA signal se-quence,The construction of pcDNA-Mwj is the key...

A pair of specific primers were desigend and synthesized according to the published sequence of M gene of IBV in GeneBank.cDNA of M gene was obtained by RT-PCR from viral RNA of IBVSAIBWJ strain,and further cloned into PGEM-T vector,and identified the correct plasmid by PCR and double digestion.The M gene was sub-cloned into pcDNA3.1(+)plasmid be-tween HindⅢand XhoⅠsites to construct the IBV M gene expressing plasmid with CVM promoter and BGH polyA signal se-quence,The construction of pcDNA-Mwj is the key step in the course of construction of DNA vaccine N gene and its use.

膜蛋白是禽传染性支气管炎病毒的主要结构蛋白,可能存在着具有重要免疫作用的抗原决定簇,能诱导细胞介导的免疫反应,但其免疫生物学功能尚不清楚。本研究选择禽传染性支气管炎病毒中国分离株SAIBWJ,通过RT-PCR扩增获得其膜蛋白基因M基因并插入到PGEM-T载体中,获得重组质粒PGEM-M,将重组质粒中的克隆片段进行序列测定及分析。对PGEM-M进行双酶切获得M基因片段,克隆到真核表达载体pcDNA3.1+中,通过菌落PCR、双酶切证明获得了M基因的真核表达质粒。利用DNAstar软件将IBVSAIBwj株的膜蛋白基因的核苷酸序列与GenBank中的国内外参考毒株进行比较,其核苷酸序列的同源率为91.6%~98.8%,氨基酸序列的同源率为91.6%~99.6%。糖基化位点的增加和减少对M蛋白的抗原性产生很大的影响。在M蛋白近N端含有两个糖基化位点,第一个跨膜疏水区距离亲水区大约20个氨基酸。SAIBwjM基因核苷酸、氨基酸进化关系可以看出,SAIBwjM与国内外参考株的同源性均较高,因此可能具有交叉保护性抗原,可望用于制备诊断试剂及制备基因工程疫苗。由于IBV变异较大,常规疫苗不能产生完全保护力。在I...

膜蛋白是禽传染性支气管炎病毒的主要结构蛋白,可能存在着具有重要免疫作用的抗原决定簇,能诱导细胞介导的免疫反应,但其免疫生物学功能尚不清楚。本研究选择禽传染性支气管炎病毒中国分离株SAIBWJ,通过RT-PCR扩增获得其膜蛋白基因M基因并插入到PGEM-T载体中,获得重组质粒PGEM-M,将重组质粒中的克隆片段进行序列测定及分析。对PGEM-M进行双酶切获得M基因片段,克隆到真核表达载体pcDNA3.1+中,通过菌落PCR、双酶切证明获得了M基因的真核表达质粒。利用DNAstar软件将IBVSAIBwj株的膜蛋白基因的核苷酸序列与GenBank中的国内外参考毒株进行比较,其核苷酸序列的同源率为91.6%~98.8%,氨基酸序列的同源率为91.6%~99.6%。糖基化位点的增加和减少对M蛋白的抗原性产生很大的影响。在M蛋白近N端含有两个糖基化位点,第一个跨膜疏水区距离亲水区大约20个氨基酸。SAIBwjM基因核苷酸、氨基酸进化关系可以看出,SAIBwjM与国内外参考株的同源性均较高,因此可能具有交叉保护性抗原,可望用于制备诊断试剂及制备基因工程疫苗。由于IBV变异较大,常规疫苗不能产生完全保护力。在IBV的免疫保护机制中,细胞免疫起着关键的作用,近年来一些研究者已经进行了IBVDNA疫苗的研究,并取得了一定的进展。IBV的S1基因真核表达质粒可同时诱?

That genetic diseases, including single nucleotide substitution and chromosomal aberrant, are individual sacrifices needed for species evolution for quite a while. Based on this concept, genetic diseases can be used as the most precious in vivo experiment occurred naturally at the research point of view. By focusing on the leading mutations, single nucleotide substitution caused missense change, we recognized that for missense there is a biased G→A over C→T both at certain individual genes and at genome level....

That genetic diseases, including single nucleotide substitution and chromosomal aberrant, are individual sacrifices needed for species evolution for quite a while. Based on this concept, genetic diseases can be used as the most precious in vivo experiment occurred naturally at the research point of view. By focusing on the leading mutations, single nucleotide substitution caused missense change, we recognized that for missense there is a biased G→A over C→T both at certain individual genes and at genome level. Furthermore, there is a C→T over G→A bias in nonsense mutations. The underlying mechanism of these observed mutation biases in evolution are discussed.

甲基化造成的大量C→T转换,为进化提供了源源不断的突变,而同时其所编码的氨基酸则逐渐丧失了遗传上的保守性。与此相反,由G至A的原发性转换可能甚少,相应密码子所对应的氨基酸则相对稳定,从而使可避免甲基化的GDN和DGN三联密码成为保守氨基酸进化的必要条件。

 
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