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基因i型
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  genotype i
     Conclusion All the 4 strains prevalent in China were genotype I.
     结论4株RV均属于基因I型
短句来源
     Phylogenetic tree based on the M genes showed that most domestic strains belong to CCoV genotype II, while Fox3-1and Rac2-1 are classified into genotype I.
     基于M基因的遗传进化分析表明,目前国内绝大多数CCoV流行毒株都属于CCoV基因II型,只有Fox3-1和Rac2-1两个毒株属于基因I型
短句来源
  “基因i型”译为未确定词的双语例句
     but also poison and different genotype. At present,classical vaccine strain(genotypeI、II) were I、II(B1)、III(F)、IV(Lasota)and V4,ect.
     我国现行的疫苗株为I系、II系(B1)、III系(F)、IV系(Lasota)和V4株等,均属于经典型(基因I型、II型)。
短句来源
     Finally, the human myelin protein zero like gene isoform I and II ( MPZL 1a, MPZL 1b; GenBank: AF095727, AF092424) were cloned.
     在所得cDNA上再设计引物进行巢式PCR ,最终克隆了人髓鞘蛋白零样基因I型和II型 (MPZL1a、MPZL1b ,GenBank :AF0 95 72 7、AF0 92 42 4)。
短句来源
     The mean homology between strains of these two groups was 76.7/83.2% for the nucleotide/amino acid sequnces, while it was 79.9/89.4% and 90.0/86.7% within group Ia and Ib respectively.
     核苷酸序列的同源性及系统树分析的结果表明,国内的BVDV毒株存在Ia和Ib2个基因亚型,它们均属基因I型BVDV
短句来源
     The subjects were classified,according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene ,as Ⅱ, DD,and heterozygotes for deletion/insertion(DI).
     60例妊高征患者中,ACE基因I型和DD型频率分别为15%(9/60)和65%(39/60),而38例正常晚妊妇女中则分别为50%(19/38)和10.5%(4/38)。
短句来源
     The expression of osteogenesis representative gene: I Collagen, Osteocalcin, bone sialoprotein (BSP), alkaline phosphatase (AKP), and Cbfa1 mRNA were detected with reverse transcription-polymerase chain reaction (RT-PCR). The expression of osteocalcin was detected with immunocytochemical stain. The expression of Cbfa protein was detected with Westernblot.
     采用脂质体转染技术行核心结合因子α1基因转染NIH3T3成纤维细胞,成骨标志基因I型胶原、骨钙素、骨唾液蛋白、碱性磷酸酶、核心结合因子α1mRNA的表达采用反转录-聚合酶链反应检测,骨钙素的表达采用免疫细胞化学染色检测,Cbfa蛋白的表达采用Westernblot检测。
短句来源
  相似匹配句对
     HCV gene was typeⅡ.
     HCV基因为Ⅱ
短句来源
     coli, but a single-base exchange (A to G) existed in the-35 box of PD-67.The trpEG genes from the wild-type strain and its mutant were expressed both in C.
     野生和突变AS基因在C.
短句来源
     4 new genes were obtained.
     U基因
短句来源
     (2)C shape;
     C ;
短句来源
     (3)D shape.
     D
短句来源
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  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
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The foreign sequence insertion region within P125 gene of three cytopathic (cp) strains (184, H and D) and one noncytopathic (ncp) strain (Yak) of bovine viral diarrhea virus were amplified by the reverse transcription polymerase chain reaction (RT PCR), cloned and sequenced. The results confirmed that the three cp strains possess neither any insertion nor gene rearrangement, gene recombination, gene deletion in the inserting region of the P125 gene as observed in many other cp strains. However some nucleotide...

The foreign sequence insertion region within P125 gene of three cytopathic (cp) strains (184, H and D) and one noncytopathic (ncp) strain (Yak) of bovine viral diarrhea virus were amplified by the reverse transcription polymerase chain reaction (RT PCR), cloned and sequenced. The results confirmed that the three cp strains possess neither any insertion nor gene rearrangement, gene recombination, gene deletion in the inserting region of the P125 gene as observed in many other cp strains. However some nucleotide and amino acid substitutions were found in the region analysed. Following the sequence homology, as well as phylogenic analysis, the 4 strains of BVDV could be divided into two subgenotypic groups, the Ia and the Ib. The mean homology between strains of these two groups was 76.7/83.2% for the nucleotide/amino acid sequnces, while it was 79.9/89.4% and 90.0/86.7% within group Ia and Ib respectively.

根据牛病毒性腹泻病毒(BVDV)NADL株的序列,以计算机辅助设计,化学合成1对引物(W1/W2);应用RT-PCR对国内不同地区分离的4株BVDV的P125基因外源序列插入区进行了扩增,并将扩增的片段进行克隆和序列测定,同时以DNASIS和PROSIS计算机软件将测定的核苷酸序列及推导的氨基酸序列进行比较分析。结果证实,国内分离的4株BVDV在这一区域中既没有外源序列的插入,也没有基因重组、基因重排或基因缺失,但存在某些核苷酸和氨基酸的替换,表明病毒的致细胞病变作用除与外源序列的插入、基因重组、基因重排或基因缺失有关外,可能还存在其他机制。核苷酸序列的同源性及系统树分析的结果表明,国内的BVDV毒株存在Ia和Ib2个基因亚型,它们均属基因I型BVDV

To establish a method for sensitive,specific and rapid detection of the genotypes of angiotensin converting enzyme (ACE) gene and to study the relationship between the ACE gene polymorphism and preeclampsia, a pair of primers for the exon 16 of ACE gene was designed, and a sensitive and specific method for detection of the ACE region of the insertion/deletion was determined by polymerase chain reaction(PCR). The subjects were classified,according to the presence or absence of a 287bp insertion in intron 16 of...

To establish a method for sensitive,specific and rapid detection of the genotypes of angiotensin converting enzyme (ACE) gene and to study the relationship between the ACE gene polymorphism and preeclampsia, a pair of primers for the exon 16 of ACE gene was designed, and a sensitive and specific method for detection of the ACE region of the insertion/deletion was determined by polymerase chain reaction(PCR). The subjects were classified,according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene ,as Ⅱ, DD,and heterozygotes for deletion/insertion(DI).The study reveals genotype Ⅱ of ACE is a marker for reduced risk for preeclampsia while DD is a risk gene.

为探讨血管紧张素Ⅰ转化酶(ACE)基因第16内含子插入/缺失多态性与妊娠高血压综合征的关系,应用聚合酶链反应技术,检测60例妊高征患者及38例正常妊娠妇女ACE基因的插入/缺失状态。60例妊高征患者中,ACE基因I型和DD型频率分别为15%(9/60)和65%(39/60),而38例正常晚妊妇女中则分别为50%(19/38)和10.5%(4/38)。提示I型基因是妊高征的保护性基因,DD型基因是妊高征的易感基因

Objective To establish a method for sensitive specific and rapid detection of the angiotensin converting enzyme(ACE) genotypes and to study the relationship between the ACE gene polymorphism and preeclampsia. Methods Sixty patients with preeclampsia and 76 normal pregnant women as controls were investigated. A pair of primers, for the intron 16 of ACE gene was designed. A sensitive and specific method for detecting the ACE polymorphism of the insertion/deletion was established. Determined by polymerase chain...

Objective To establish a method for sensitive specific and rapid detection of the angiotensin converting enzyme(ACE) genotypes and to study the relationship between the ACE gene polymorphism and preeclampsia. Methods Sixty patients with preeclampsia and 76 normal pregnant women as controls were investigated. A pair of primers, for the intron 16 of ACE gene was designed. A sensitive and specific method for detecting the ACE polymorphism of the insertion/deletion was established. Determined by polymerase chain reaction(PCR),a 490bp(I) and 190bp(D) PCR product was identified, corresponding to the PCR amplification of the allele with or without the insertion. Results The subjects were classified, according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene, as II, DD, or heterozygotes for deletion/insertion(DI). The frequency of allele gene(0.75) and the percentage of the ACE DD genotype(65%) in the preeclampsia group were significantly higher as compared with the freqency(0.308) and the percentage (10.5%) in the control group respectively. Conclusion Genotype II of ACE is a marker for reduced risk for preeclampsia and DD is a risk gene.

目的探讨血管紧张素Ⅰ转化酶(ACE)基因第16内含子插入/丢失多态性与妊娠高血压综合征(简称妊高征)的关系。方法应用聚合酶链反应,检测60例妊高征患者及对照组76例正常孕妇的ACE基因中第16内含子是否有Alu重复结构存在。结果60例妊高征患者中ACE基因I型和DD型频率分别为15%(9/60)和65%(39/60),而76例正常晚期妊娠妇女中则分别为50%(38/76)和10.5%(8/76)。妊高征组的缺失型(D型)ACE等位基因出现频率为0.75,高于对照组的0.308,差异有显著性(P<0.01)。结论提示I型基因是妊高征的保护性基因,DD型基因是妊高征的易感基因,ACE基因的缺失多态性(DD)可能与妊高征的发病有关。

 
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