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基因i型
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  genotype i
    Phylogenetic tree based on the M genes showed that most domestic strains belong to CCoV genotype II, while Fox3-1and Rac2-1 are classified into genotype I.
    基于M基因的遗传进化分析表明,目前国内绝大多数CCoV流行毒株都属于CCoV基因II型,只有Fox3-1和Rac2-1两个毒株属于基因I型
短句来源
  genotype i
    Phylogenetic tree based on the M genes showed that most domestic strains belong to CCoV genotype II, while Fox3-1and Rac2-1 are classified into genotype I.
    基于M基因的遗传进化分析表明,目前国内绝大多数CCoV流行毒株都属于CCoV基因II型,只有Fox3-1和Rac2-1两个毒株属于基因I型
短句来源
  genotype i
    Phylogenetic tree based on the M genes showed that most domestic strains belong to CCoV genotype II, while Fox3-1and Rac2-1 are classified into genotype I.
    基于M基因的遗传进化分析表明,目前国内绝大多数CCoV流行毒株都属于CCoV基因II型,只有Fox3-1和Rac2-1两个毒株属于基因I型
短句来源
  “基因i型”译为未确定词的双语例句
    but also poison and different genotype. At present,classical vaccine strain(genotypeI、II) were I、II(B1)、III(F)、IV(Lasota)and V4,ect.
    我国现行的疫苗株为I系、II系(B1)、III系(F)、IV系(Lasota)和V4株等,均属于经典型(基因I型、II型)。
短句来源
    Finally, the human myelin protein zero like gene isoform I and II ( MPZL 1a, MPZL 1b; GenBank: AF095727, AF092424) were cloned.
    在所得cDNA上再设计引物进行巢式PCR ,最终克隆了人髓鞘蛋白零样基因I型和II型 (MPZL1a、MPZL1b ,GenBank :AF0 95 72 7、AF0 92 42 4)。
短句来源
    The mean homology between strains of these two groups was 76.7/83.2% for the nucleotide/amino acid sequnces, while it was 79.9/89.4% and 90.0/86.7% within group Ia and Ib respectively.
    核苷酸序列的同源性及系统树分析的结果表明,国内的BVDV毒株存在Ia和Ib2个基因亚型,它们均属基因I型BVDV
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  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
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  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
更多          
  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
更多          


The foreign sequence insertion region within P125 gene of three cytopathic (cp) strains (184, H and D) and one noncytopathic (ncp) strain (Yak) of bovine viral diarrhea virus were amplified by the reverse transcription polymerase chain reaction (RT PCR), cloned and sequenced. The results confirmed that the three cp strains possess neither any insertion nor gene rearrangement, gene recombination, gene deletion in the inserting region of the P125 gene as observed in many other cp strains. However some nucleotide...

The foreign sequence insertion region within P125 gene of three cytopathic (cp) strains (184, H and D) and one noncytopathic (ncp) strain (Yak) of bovine viral diarrhea virus were amplified by the reverse transcription polymerase chain reaction (RT PCR), cloned and sequenced. The results confirmed that the three cp strains possess neither any insertion nor gene rearrangement, gene recombination, gene deletion in the inserting region of the P125 gene as observed in many other cp strains. However some nucleotide and amino acid substitutions were found in the region analysed. Following the sequence homology, as well as phylogenic analysis, the 4 strains of BVDV could be divided into two subgenotypic groups, the Ia and the Ib. The mean homology between strains of these two groups was 76.7/83.2% for the nucleotide/amino acid sequnces, while it was 79.9/89.4% and 90.0/86.7% within group Ia and Ib respectively.

根据牛病毒性腹泻病毒(BVDV)NADL株的序列,以计算机辅助设计,化学合成1对引物(W1/W2);应用RT-PCR对国内不同地区分离的4株BVDV的P125基因外源序列插入区进行了扩增,并将扩增的片段进行克隆和序列测定,同时以DNASIS和PROSIS计算机软件将测定的核苷酸序列及推导的氨基酸序列进行比较分析。结果证实,国内分离的4株BVDV在这一区域中既没有外源序列的插入,也没有基因重组、基因重排或基因缺失,但存在某些核苷酸和氨基酸的替换,表明病毒的致细胞病变作用除与外源序列的插入、基因重组、基因重排或基因缺失有关外,可能还存在其他机制。核苷酸序列的同源性及系统树分析的结果表明,国内的BVDV毒株存在Ia和Ib2个基因亚型,它们均属基因I型BVDV

To clone novel myelin protein related genes, two human ESTs, which shared significant similarity with the human myelin protein zero gene, were found by the comparison of homologue between the cDNA coding region sequences of MPZ gene and the EST database of NCBI. An 801 bp EST contig was assembled, which was 100% identical with a 128 kb genomic sequence, mapped to 1q24. A 435 bp open reading frame (ORF) within the 801 bp contig was shown by computer analysis. Two primers, designed according to the sequence...

To clone novel myelin protein related genes, two human ESTs, which shared significant similarity with the human myelin protein zero gene, were found by the comparison of homologue between the cDNA coding region sequences of MPZ gene and the EST database of NCBI. An 801 bp EST contig was assembled, which was 100% identical with a 128 kb genomic sequence, mapped to 1q24. A 435 bp open reading frame (ORF) within the 801 bp contig was shown by computer analysis. Two primers, designed according to the sequence of the contig, were coupled with the primers(λgt10 5 and gt10 5) on the sequences flanking cloning site of the cDNA library vector to amplify the cDNA library sequences by nested PCR. New primers, designed based on novel cDNA sequences, were used for the PCR amplification with λgt10 5 and gt10 5 in the same way as above. Finally, the human myelin protein zero like gene isoform I and II ( MPZL 1a, MPZL 1b; GenBank: AF095727, AF092424) were cloned. Comparison of gene and protein structures between MPZL1 and MPZ revealed that MPZL 1 is the second member of MPZ family. Mutation analysis of MPZL1 gene was performed in 24 Charcot Marie Tooth disease (CMT) families and 26 nonsyndrome deafness families, but no mutation was found.

为克隆新的髓鞘蛋白相关基因 ,将MPZ基因编码区cDNA序列与EST数据库进行同源性比较 ,得到与MPZ显著相似的 2个EST ,构建成 80 1bp的重叠群。此重叠群与定位在 1q2 4的 12 8kbgDNA的相似性为 10 0 %。计算机分析表明 80 1bp的重叠群可能存在一个 435bp的阅读框架。在重叠群上设计两个引物与文库载体臂上的引物配对 ,扩增各种cDNA文库DNA作巢式PCR。在所得cDNA上再设计引物进行巢式PCR ,最终克隆了人髓鞘蛋白零样基因I型和II型 (MPZL1a、MPZL1b ,GenBank :AF0 95 72 7、AF0 92 42 4)。对MPZL与MPZ的基因结构和推导的蛋白质一级结构进行分析与比较 ,证明MPZL1是MPZ家族的第二个成员。对 2 4个腓骨肌萎缩症家系和 2 6个非综合征型耳聋家系进行了MPZL1基因的突变检测 ,没有发现突变

The amplify important function region of the fusion gene of 37 isolates of Newcastle Disease virus was employed to by One step RT-PCR The deduced Amino-acid sequence analysis and The genotypes characteristic showed that 24 strains belong to genotype VII; 2 strains belong to genotype III;one strain belongs to genotype VI; one strain belongs to genotype I; 6 strains belong to genotype II ;other three strains are hybrid of genotype VII and genotype VI through the analysis of the deduced Aminoacid sequences,but...

The amplify important function region of the fusion gene of 37 isolates of Newcastle Disease virus was employed to by One step RT-PCR The deduced Amino-acid sequence analysis and The genotypes characteristic showed that 24 strains belong to genotype VII; 2 strains belong to genotype III;one strain belongs to genotype VI; one strain belongs to genotype I; 6 strains belong to genotype II ;other three strains are hybrid of genotype VII and genotype VI through the analysis of the deduced Aminoacid sequences,but from the Phylogenetic tree,SD054 and SD069 belong to genotype VI,SD052 belong to genotype VIIWe can see,the outbreak of Newcastle Disease was very complex in China, that is, not only with the old genotype,but also with the new genotypeMeanwhile, This rpeport found that three strains was the hybrid of genotype VII and VI

利用一步法RT -PCR技术成功扩增了 37个新城疫病毒分离株的 (其中 2株属于鸽源病毒 )F基因片段 (约5 0 0bp) ,通过对分离株的测序和基因分型表明 ,2 4株属于基因VII型 ,1株属于基因VI型 ,2株属于基因III型 ,1株属于基因I型 ,6株属于基因II型 ,另外有 3株从氨基酸方面分析是基因VII和VI型的杂交体 ,但从进化树方面 ,分离毒SD0 5 4和SD0 6 9属于基因VI型 ,SD0 5 2属于基因VII型。可以看出 ,我国新城疫的流行是极其复杂的 ,既有老基因型的威胁 ,又有新基因型的不断流行 ,同时发现有 3株病毒发生了两基因型间的杂交现象

 
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