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   基因i型 在 基础医学 分类中 的翻译结果: 查询用时:0.839秒
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基因i型
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  genotype i
    Conclusion All the 4 strains prevalent in China were genotype I.
    结论4株RV均属于基因I型
短句来源
  genotype i
    Conclusion All the 4 strains prevalent in China were genotype I.
    结论4株RV均属于基因I型
短句来源
  “基因i型”译为未确定词的双语例句
    The expression of osteogenesis representative gene: I Collagen, Osteocalcin, bone sialoprotein (BSP), alkaline phosphatase (AKP), and Cbfa1 mRNA were detected with reverse transcription-polymerase chain reaction (RT-PCR). The expression of osteocalcin was detected with immunocytochemical stain. The expression of Cbfa protein was detected with Westernblot.
    采用脂质体转染技术行核心结合因子α1基因转染NIH3T3成纤维细胞,成骨标志基因I型胶原、骨钙素、骨唾液蛋白、碱性磷酸酶、核心结合因子α1mRNA的表达采用反转录-聚合酶链反应检测,骨钙素的表达采用免疫细胞化学染色检测,Cbfa蛋白的表达采用Westernblot检测。
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  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
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  genotype i
This suggests that viruses belonging to the genotype I widely distribute among various fish species in many Asian countries.
      
One of the variants was identified as genotype I, the other as genotype III of HEV.
      
The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
      
In untreated patients (2 genotype I, 6 genotype II, 9 mixed infections) 4 out of 9 mixed infections selected for genotype II at the end of follow-up.
      
Of the nonresponders 18/24 had more than one genoytpe, 5 were genotype II at baseline and one had genotype I.
      
更多          


Objective To explore the gene variation of rabies virus (RV) prevalent in China and evaluate the availability of the current vaccine. Methods Amplify the G gene as well as the gene between G-L and G-M intergenic regions of 4 street virus strains of RV by RT-PCR and identify the PCR products by direct sequencing. Compare the nucleotide and deduced amino acid sequences of the 4 strains with those of strains reported by computer software. Results The coding region of G gene consisted of 1 575 nucleotides. The homologies...

Objective To explore the gene variation of rabies virus (RV) prevalent in China and evaluate the availability of the current vaccine. Methods Amplify the G gene as well as the gene between G-L and G-M intergenic regions of 4 street virus strains of RV by RT-PCR and identify the PCR products by direct sequencing. Compare the nucleotide and deduced amino acid sequences of the 4 strains with those of strains reported by computer software. Results The coding region of G gene consisted of 1 575 nucleotides. The homologies of the amino acid sequences of G proteins of the 4 strains to those of reported strains (except Mokola strain) were significantly higher than those of their corresponding nucleotide sequences. The homologies of 4 street virus strains to CTN were significantly higher than those to aG strain. Compared with the sequences at intracellular regions, the homologies of the sequences at extracellular regions of the 4 strains to those of reported strains were significantly high. The lengths of nucleotide sequences at G-M intergenic regions of the 4 strains were 5 bp. Except Guangxi 4 strain, the homology of the rest 3 strains was 100%. All the lengths of nucleotide sequences at G-L intergenic regions of the 4 strains were 24 bp, however, only the homology of Yue 1 and Feidong strains was 100% . Conclusion All the 4 strains prevalent in China were genotype I. The strains isolated in Guangxi Province might be derived from different origins. Gene variation was observed between the street virus strain and vaccine strain. The evolutionary modes of the 4 street virus strains were similar to that of SAD-B19 strains but significantly different from that of PV strain. Yue 1 and Feidong strains showed a significantly close consanguinity, so they might be evolved from the same strain.

目的探讨我国流行的狂犬病毒(RV)基因差异,评价现有疫苗的适用性。方法通过RT-PCR并对其产物测序后获得4株RV街毒株的G基因序列以及G与M和L基因的区间序列。利用计算机分析软件比较4株RV与已经发表的毒株的核苷酸和推导的氨基酸序列。结果糖蛋白(GP)完整的编码基因序列长度为1575 bp。GP氨基酸序列同源性明显高于相应的核苷酸序列(除Mokola外),4株街毒与CTN的同源性高于aG株。GP不同区段比较显示,膜外区同源性高于膜内区。G-M区间核苷酸序列长度为5 bp;除广西4外,其余毒株100%同源。GL区间核苷酸序列长度为2,4 bp,越1与肥东同源性为100%。结论4株RV均属于基因I型。广西的毒株之间存在不同的来源。街毒与疫苗株之间基因存在差异。4株RV与SAD-B19进化途径相近,与PV株相差较远。越1与肥东株亲缘关系极近,可能是由同一毒株演化而来。

AIM: To observe the fibroblast NIH3T3 transfected by the same signal molecule core -binding factor α1 (Cbfa1) gene of the osteogenesis induced by cytokine and the possibility of changes of its osteogenesis phenotype. METHODS:The experiment was done in the Central Laboratory of Department of Orthopaedics of Xinqiao Hospital of Third Military Medical University of Chinese PLA from October 2003 to October 2004. The gene trasfection of Cbfa1 to fibroblast NIH3T3 was performed with liposomes trasfection technique....

AIM: To observe the fibroblast NIH3T3 transfected by the same signal molecule core -binding factor α1 (Cbfa1) gene of the osteogenesis induced by cytokine and the possibility of changes of its osteogenesis phenotype. METHODS:The experiment was done in the Central Laboratory of Department of Orthopaedics of Xinqiao Hospital of Third Military Medical University of Chinese PLA from October 2003 to October 2004. The gene trasfection of Cbfa1 to fibroblast NIH3T3 was performed with liposomes trasfection technique. The expression of osteogenesis representative gene: I Collagen, Osteocalcin, bone sialoprotein (BSP), alkaline phosphatase (AKP), and Cbfa1 mRNA were detected with reverse transcription-polymerase chain reaction (RT-PCR). The expression of osteocalcin was detected with immunocytochemical stain. The expression of Cbfa protein was detected with Westernblot. RESULTS: ① The expression of osteocalcin, bone sialoprotein and alkaline phosphatase appeared after the trasfection of Cbfa1 gene to fibroblast NIH3T3. The reinforcement of I type collagen expression indicated that the expression of osteogenesis representative gene existed in the transcription level. ② The expression of osteocalcin was detected by immunocytochemical stain, which showed that the NIH3T3 cells had developed the osteogenesis cells. ③ The expression of Cbfa1 protein was detected by Westernblot, which confirmed that the phenotype changes had occurred on fibroblast. CONCLUSION: Gene transfer of Cbfa1 to fibroblast NIH3T3 can alter the conversion of the phenotype of osteogenesis, and then the quantity of osteoblast increased and made the increase of osteogenesis ability possible.

目的:观察细胞因子诱导成骨的共同信号分子核心结合因子α1基因转染NIH3T3成纤维细胞,其成骨表型转变的可能性。方法:实验于2003-10/2004-10在解放军第三军医大学新桥医院骨科中心实验室完成。采用脂质体转染技术行核心结合因子α1基因转染NIH3T3成纤维细胞,成骨标志基因I型胶原、骨钙素、骨唾液蛋白、碱性磷酸酶、核心结合因子α1mRNA的表达采用反转录-聚合酶链反应检测,骨钙素的表达采用免疫细胞化学染色检测,Cbfa蛋白的表达采用Westernblot检测。结果:①NIH3T3成纤维细胞经核心结合因子α1基因转染后出现骨钙素、骨唾液蛋白、碱性磷酸酶的表达,I型胶原表达增强,说明在转录水平有成骨标志基因的表达。②免疫细胞化学染色检测到了骨钙素的表达,显示NIH3T3细胞已发展为成骨细胞。③Westernblot检测到了核心结合因子α1蛋白的表达,证实了成纤维细胞发生了表型的改变。结论:核心结合因子α1基因转移NIH3T3成纤维细胞改变了成纤维细胞向成骨表型转化,增加了成骨细胞数量,使成骨能力增强成为可能。

 
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