Plant Expression Vector Containing Antifreeze Peptide Gene and Its Applications in Transgenic Plants, Secretory Expression in Trichoderma Viride and a Study on Intron Function of AFP Gene
The Study in the Intron 1 of Human Na/K-ATPase α1 Subunit and the Relationship between the Structure and Function of the 5'FS of ANP Gene Derived from Salt Sensitive Hypertension Pedigrees
It contained the complete first exon(1-61 bp),first intron(62-702 bp) and partial sequence of exon2(703-731 bp)in the 731 base pairs fragment of FSHβ gene. The content of A+T(66.07%) was higher than that of G+C(33.93%) and it coded 7 amino acids.
It includes the complete first exon(1～61?bp),first intron(62～703?bp) and partial sequence of exon 2(704～732?bp) in the 732 base pairs of FSHβ gene sequence,and the content of A+T(66.12%) is higher than G+C(33.88%).
Results The luciferase reporter gene construct containing the first intron of α 1(I) collagen gene (-804～+1 452, was called as PGL3 col) had a higher level of gene expression, as compared with the construct lacking the first intron 〔was called as PGL3 col (△intron)〕 in activated HSCs (315±45 U/mg protein vs 220±70 U/mg protein, P <0.05).
There existed three kinds of mutation in the ~12th intron of the HSG 1q82139 G/A, 82153 C/G and 82273 G/-. The 1q82139 G/A could be a nonsense mutation and there was significant difference in the 1q82153 C/G and 1q82273 G/-SNP and gene frequencies between different Chinese Han populations, which could be independent risk factors for essential hypertension.
Furthermore, characteristics of EID, such as intron phase, distribution of different splice sites, and the relationship between genome size and intron proportion or intron density, have been studied.
The exon-intron structure of these genes has been analyzed in zebrafish Danio rerio, loach Misgurnus fossilis, fugu Takifugu rubripes, and Nile puffer Tetraodon fahaka.
This is supported by high sequence similarity between mlc1 and mlc3 as well as by the exon-intron structure of these genes and their localization on different chromosomes.
The result indicated that the sequence was 1,813 bp (BamHI and SacI were introduced at the 5' and 3' end) including 7 extrons and 6 introns, coding 238 amino acids.
Studies on the statistical characteristics of EID show that there were 103, 848 genes, 478,484 introns, and 582,332 exons, with an average of 4.61 introns and 5.61 exons per gene.
Results of the statistical analysis on the data from nine model species showed that in eukaryotes, higher species do not necessarily have more introns or exons in a gene than lower species.
Furthermore, characteristics of EID, such as intron phase, distribution of different splice sites, and the relationship between genome size and intron proportion or intron density, have been studied.
The exon-intron structure of these genes has been analyzed in zebrafish Danio rerio, loach Misgurnus fossilis, fugu Takifugu rubripes, and Nile puffer Tetraodon fahaka.
This is supported by high sequence similarity between mlc1 and mlc3 as well as by the exon-intron structure of these genes and their localization on different chromosomes.
As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA.
Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene.
Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA.
内含子
intron
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