The genetic polymorphism of 5′-flanking region,1st intron,2nd exon and 5th exon of GH gene were investigated using PCR-SSCP methods in 281 horses from four local Mongolia breeds,one Chinese cultivating breed and one importing breed.
It contained the complete first exon(1-61 bp),first intron(62-702 bp) and partial sequence of exon2(703-731 bp)in the 731 base pairs fragment of FSHβ gene. The content of A+T(66.07%) was higher than that of G+C(33.93%) and it coded 7 amino acids.
Three mutation loci [78(-/A),132(G/A) and 343(G/A)]were found and they all exited in the sequence of intron1,while the correlation between mutation loci and the birth rate could not be confirmed.
The phylogenetic trees constructed by the FSHβ gene intron1 of goat and other 8 species from GenBank were not completely accorded with factual taxonomy,which may be caused by the fact that most species' gene length were not longer than 700bp and that the species relationships were beyond the congeneric limit.
It contained the complete first exon(1-61 bp),first intron(62-702 bp) and partial sequence of exon2(703-731 bp)in the 731 base pairs fragment of FSHβ gene. The content of A+T(66.07%) was higher than that of G+C(33.93%) and it coded 7 amino acids.
Three Msp I restriction enzyme sites with three alleles in the first intron (H1、 H2 and H3) were observed. H1 was the dominant allele in each strain, and its frequency was significantly higher than that of H2 and H3. There were EcoRV and Ava I restriction enzyme sites with polymorphisms in the third intron, respectively.
It includes the complete first exon(1～61?bp),first intron(62～703?bp) and partial sequence of exon 2(704～732?bp) in the 732 base pairs of FSHβ gene sequence,and the content of A+T(66.12%) is higher than G+C(33.88%).
The lengthes of the first intron and the second intron were 1 833 bp and 2 018 bp respectively. The full length of Mongolian horse MSTN gene was 4 979 bp and firstly submitted to Genbank.
According to the FSHβgene sequence of bovine and sheep in GenBank,the primers were designed and the sequence of FSHβgene in-1 from Nanjiang-Huang goat was sequenced.
In the complete 641 base pairs of FSHβgene in-1,the content of A,C,G,T was 35.73%,13.88%,17.47% and 32.92%,respectively. The content of it was significantly that the A+T (68.65%) was higher than the content of G+C (31.35%).
Through sequencing and analyzing by method of bioinformatics: SDIFN-αORF includes 576 nucleotides, encoding 191 amino acids. Molecular weight is about 21.64kDa, front 28 amino acide is signal peptide, and the cleavage site lies in 28-29 amino acide(ANA-FS).
Furthermore, characteristics of EID, such as intron phase, distribution of different splice sites, and the relationship between genome size and intron proportion or intron density, have been studied.
The exon-intron structure of these genes has been analyzed in zebrafish Danio rerio, loach Misgurnus fossilis, fugu Takifugu rubripes, and Nile puffer Tetraodon fahaka.
This is supported by high sequence similarity between mlc1 and mlc3 as well as by the exon-intron structure of these genes and their localization on different chromosomes.
As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA.
Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene.
Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA.
内含子
intron 
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