Objective: To study the expression of MIBP1, the differentiation-associated gene also known as c-myc intron binding protein 1, in specimens of cerebral glioma using real-time PCR.
The HLD gene is mapped on 13ql4.3, and the cDNA sequence has 4398 bp. This gene contains 21 exons and 20 introns. The length of exons ranged from 77bp to 1234bp, mostly about 200bp.
Results In “central deletion hot spot”, about 30.8% of breakpoints fell in intron 44, about 23.1%, 17.9%, 10.3%, 10.3% of breakpoints fell in introns 50,51, 45, 48, respectively.
METHODS:Multiplex PCR and(CA)n polymorphism(introns 44,49 and 50) methods were used to diagnose the DNA depletion of dystrophy gene in BMD carriers and BMD fetus.
Methods The plasma PAI 1 activity, by means of chromgenic substrate assay, and the sequence polymorphisms of 4G/5G in promotor region and (CA)n dinucleotide repeats in the 4th intron of PAI 1 gene, by amplified fragment length polymorphism assay, were measured in 50 patients with first ever cerebral infarction (FCI), 45 patients with RCI and 60 healthy controls.
Objective: To study the relationship of ACE gene quantitative trait loci(QTLs):I/D in 16th intron and 4656 (CT)2/3 in 3 untranslation region (UTR) polymorphisms tohypertension and cerebrovascular diseases.
Method Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron-1 (+83 bp).
Furthermore, characteristics of EID, such as intron phase, distribution of different splice sites, and the relationship between genome size and intron proportion or intron density, have been studied.
The exon-intron structure of these genes has been analyzed in zebrafish Danio rerio, loach Misgurnus fossilis, fugu Takifugu rubripes, and Nile puffer Tetraodon fahaka.
This is supported by high sequence similarity between mlc1 and mlc3 as well as by the exon-intron structure of these genes and their localization on different chromosomes.
The result indicated that the sequence was 1,813 bp (BamHI and SacI were introduced at the 5' and 3' end) including 7 extrons and 6 introns, coding 238 amino acids.
Studies on the statistical characteristics of EID show that there were 103, 848 genes, 478,484 introns, and 582,332 exons, with an average of 4.61 introns and 5.61 exons per gene.
Results of the statistical analysis on the data from nine model species showed that in eukaryotes, higher species do not necessarily have more introns or exons in a gene than lower species.
We have investigated the frequency distribution, across a broad range of geographically dispersed populations, of alleles of the polymorphicAlu insertion that occurs within the 8th intron of the tissue plasminogen activator gene (PLAT).
No nucleotide mutation in two important regions of the presumptive promoter for tr-kit mRNA was detected within the 16th intron of the mouse strains examined.