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hbcag基因
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  hbcag gene
     The potential possibility of the recombinant protein in treating hepatic fibrosis was also investigated. METHODS: After three kind of TGFP1 Antigen genes (A, B, C) were ligated into Plasmid pThioHis A with HBcAg gene by T4 ligase, Recombinant plasmids were transformed into DH5α strain.
     方法:在将已插入HBcAg基因的质粒载体上,分别与TGF-β1三种抗原编码基因(A、B、C)连接,用DH5α菌转化重组质粒后,分别通过限制性核酸内切酶酶切和PCR方法鉴定;
     A Study of Hepatitis B virus core antigen synthession in E. coli High level expression of HBcAg gene in the cytoplasmic and membrane fraction of E.coli
     大肠杆菌合成的乙型肝炎病毒核心抗原的研究 HBcAg基因在大肠杆菌内和膜上表达
短句来源
     To study expression and characterization of Hepatitis B core antigen(HBcAg) protein in methyltrophic yeast,Pichia pastoris,HBcAg gene (L gene) was cloned into the intracellular expression vector pPIC3K by PCR.
     为研究乙肝核心抗原蛋白 (HBcAg)在甲醇型酵母 (PichiaPastoris)中的表达和性质 ,用PCR方法将HBcAg基因 (L)克隆到P .
短句来源
     METHODS:The HBcAg gene was amplified by polymerase chain reaction (PCR) and HBcAg bait plasmid was constructed with yeast-two hybrid system 3, then transformed into yeast AH109. The transformed yeast mated with yeast Y187 containing liver cDNA library plasmid in 2×YPDA medium.
     方法:用多聚酶链反应(PCR)法扩增HBcAg基因,连接入酵母表达载体pGBKT7中构建诱饵质粒,转化酵母细胞AH109并在其内表达,然后与转化了人肝cDNA文库质粒pACT2的酵母细胞Y187进行配合,在营养缺陷型培养基和X-α-半乳糖X-α-gal)上进行双重筛选阳性菌落,PCR从中扩增出目的片段并测序,进行生物信息学分析。
短句来源
     In the study of antigenic conversion from HBcAg to HBeAg cells from E. coli MM 206 carrying a recombinant plasmid containing HBcAg gene was used.
     本文以带有HBcAg基因重组质粒的大肠杆菌转化株Ecoli MM206所合成的HBcAg进行HBcAg转化为HBeAg的探索研究。
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  “hbcag基因”译为未确定词的双语例句
     The expression rates of hepatitis B core gene at 48, 72, 96 and 120 hours were 31.58%, 55.80%, 54.18% and 56.99%,respectively.
     HBcAg基因转染DC后48、72、96、120h的HBcAg表达率分别为31.58%、55.80%、54.18%、56.99%。
短句来源
     Expression of O21-O14-A21 gene in transformed lettuce plants was proved by RT-PCR analyses.
     RT-PCR检测初步表明,O21-O14-A21-HBcAg基因可以在生菜中正常转录表达。
短句来源
     Expression of O_(21)-O_(14)-A_(21) gene in transformed lettuce plants was proved by RT-PCR(analyses).
     RT-PCR检测初步表明,O21-O14-A21-HBcAg基因可以在生菜中正常转录表达。
短句来源
     All of the recombinant plasmids constructed in this study can be used in clinical trial for further investigation. DNA fragment of HBsAg,PreS2-HBsAg, HBsAg-enhancer I , HBcAg and enhancer II -PreC-HBcAg region of HBV were amplified by PCR using the complete genome DNA of HBV adr subtype, and inserted into VR1012 vectors, respectively .
     本研究采用常规PCR法从adr亚型HBV全基因DNA序列中分别扩增HBsAg,PreS2-HBsAg,HBsAg—ENH Ⅰ,PreS2-HBsAg-ENH Ⅰ,HBcAg和ENH Ⅱ-PreC-HBcAg基因片段,重组到载体VR1012中,构建6种HBV重组质粒(重组质粒是乙肝DNA疫苗的主体),转染HepG2细胞及COS-7细胞,免疫BALB/C小鼠及HBV转基因鼠。
短句来源
     were inserted into HBcAg el_loop,and HBV C144,CS1 and CS1S2 chimeric genes were constructed. The genes were then subcloned into pQE_30 vector,respectively,and expressed in E. coli M15.The recombinant proteins were purified by Ni 2+ _chelating affinity chromatography,and their antigenicities were identified by ELISA and Western_blot.
     表位基因插入HBcAg基因中 ,得到HBVC14 4 ,CS1,CS1S2融合基因 ,分别克隆到原核表达载体pQE_30中 ,在大肠杆菌 (E .coli)M15中进行表达 ,用Ni2 + 固相化的螯合SepharoseFastFlow亲和层析纯化重组蛋白 ,最后进行抗原性的鉴定。
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Genetic Information Carrier Gene
     基因简史
短句来源
     Induction of Immune Response Against Hepatitis B Virus by HBcAg Gene-modified Dendritic Cells Vaccine
     HBcAg基因修饰的DC疫苗抗HBV的实验研究
短句来源
     Screening of HBcAg interacting proteins in hepatocytes with yeast-two hybrid technique
     酵母双杂交技术筛选HBcAg肝细胞结合蛋白基因
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  hbcag gene
This mechanism may imply that the regulation of the X gene promoter also affects the downstream HBcAg gene promoter.
      


HBcAg was synthesized in E. coli using recombinant DNA technology. The antigen in crude bacterial lysate was a satisfactory diagnostic reagent when used in ELISA for detecting antibodies to HBcAg in serum samples.It correlated well with results obtained by using HBcAg extracted from human liver and those from corresponding Abbott kit. When compared with routine laboratory diagnosis test using only RPHA for HBsAg, it gave 8.9% and 16.5% higher positive cases (serum dilution 1:100)in screening serum samples from...

HBcAg was synthesized in E. coli using recombinant DNA technology. The antigen in crude bacterial lysate was a satisfactory diagnostic reagent when used in ELISA for detecting antibodies to HBcAg in serum samples.It correlated well with results obtained by using HBcAg extracted from human liver and those from corresponding Abbott kit. When compared with routine laboratory diagnosis test using only RPHA for HBsAg, it gave 8.9% and 16.5% higher positive cases (serum dilution 1:100)in screening serum samples from 2040 blood donors and 158 clinically suspected hepatitis patients respectively. The crude antigen can be purified by one step affinity chromatography separation.

以HBcAg的基因重组到能表达的大肠杆菌MM206株,制备了大肠菌HBcAg的粗提物。用酶联免疫吸附检测时只与抗-HBc发生特异的免疫学反应,与人肝HBcAg进行对比性平行检测78份血清标本,结果完全一致,与Abbott药盒检测抗-HBc的结果相比较,总符合率为97.3%。用于筛选献血员,阳性检出率较只测HBsAg高8.9%,对临床肝炎病人的阳性检出率也较只测HBsAg高16.5%。粗制HBcAg经亲和层析可一步纯化到电泳一条主带上。对该抗原应用情况和纯化问题进行了讨论。

By means of recombinant DNA technique the HBcAg gene from a cloned HBV genome was incorporated into and expressed in an E. coli strain. Using the bacterial lysate from this engineered strain we developed ELISA methods for the detection of serum anti-HBc and anti-HBc-IgM. In a parallel assay with Abbott kit of the same kind, assay results of 38 serum samples for anti-HBc (among them 23 were positive and 15 were negative according to Abbott kit) showed discrepancy only in one weakly positive sample with a relative...

By means of recombinant DNA technique the HBcAg gene from a cloned HBV genome was incorporated into and expressed in an E. coli strain. Using the bacterial lysate from this engineered strain we developed ELISA methods for the detection of serum anti-HBc and anti-HBc-IgM. In a parallel assay with Abbott kit of the same kind, assay results of 38 serum samples for anti-HBc (among them 23 were positive and 15 were negative according to Abbott kit) showed discrepancy only in one weakly positive sample with a relative concordance rate of 97.3%. Another comparative test with 40 serum samples (20 were positive and 20 were negative according to Abbott kit) showed complete agreement in the detection of anti-HBc-IgM.

采用基因工程技术,使克隆到大肠杆菌质粒上的 HBV 基因,经过体外切割重组,使其中的 HBcAg基因能在大肠杆菌中表达。以这种工程菌裂解液作为抗原,建立了检测血清中抗-HBc 和抗-HBcIgM的 ELISA方法。在与用美国Abbott厂的同类试剂盒进行的对比测定中,38份标本的抗 HBc检测结果,除 1份外,22份阳性和 15份阴性全部符合。40价标本抗HBcIgM检测结果,20份阳性和20份阴性亦完全相符。

In the study of antigenic conversion from HBcAg to HBeAg cells from E.coli MM 206 carrying a recombinant plasmid containing HBcAg gene was used. After two days dialysis against saline, the HBcAg in the bacterial lysate was partially converted into HBeAg.Further conversion was achieved by treating the bacterial lysate or partially purified HBcAg with β-mercaptoethano1.No appreciable change was found in the molecular size of HBcAg after reduction.The result demonstrated that HBeAg is derived from HBcAg and the...

In the study of antigenic conversion from HBcAg to HBeAg cells from E.coli MM 206 carrying a recombinant plasmid containing HBcAg gene was used. After two days dialysis against saline, the HBcAg in the bacterial lysate was partially converted into HBeAg.Further conversion was achieved by treating the bacterial lysate or partially purified HBcAg with β-mercaptoethano1.No appreciable change was found in the molecular size of HBcAg after reduction.The result demonstrated that HBeAg is derived from HBcAg and the preparation obtained could be used as the diagnostic reagent to detect anti-HBe. For blocking the released SH groups, iodoacetamide was used to perform the carboxymethylation of the reduced protein.The carboxymethylated reduced HBeAg may be separated from most of the bacterial proteins by gel filtration amd no HBcAg activity could be detected in ELISA assay.

本文以带有HBcAg基因重组质粒的大肠杆菌转化株Ecoli MM206所合成的HBcAg进行HBcAg转化为HBeAg的探索研究。菌经超声破碎获得的菌裂解液对生理盐水透析两天后,酶联检测发现抗原性部分转化为HBeAg。将菌裂解液或HBcAg精制品用2-巯基乙醇处理,可使抗原性发生进一步转化。但是分子筛层析证明抗原蛋白分子大小没有明显变化。这种制品有可能作为诊断试剂用以检测抗-HBe,而且实验结果表明HBeAg是由HBeAg衍变来的。为要提高HBeAg的稳定性,以碘乙酰胺处理,使还原的抗原蛋白通过羧甲基化反应封闭游离的巯基。经上述处理的HBeAg通过分子筛层析可与大部分细菌杂蛋白分开,制品只有HBeAg活性而测不到HBeAg活性,因而提高了抗原蛋白的稳定性与纯度。

 
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