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细胞动力学效应
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  cytokinetic effect
     THE CYTOKINETIC EFFECT OF 2-KETO-3-ETHOXYBUTYRALDEHYDE BIS (THIOSEMICARBAZONE) ON W256 CELL
     β-乙氧基-α正丁酮醛双缩氨硫脲(KTS)的细胞动力学效应
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  “细胞动力学效应”译为未确定词的双语例句
     METHODS: The peripheral blood and umbilical cord blood were harvested from 16 subjects. The lymphocytes were isolated by density gradient centrifugation and suspended in medium with CD3 mAb, rIL-2 and IFN- γ as inducing agents to prepare CIK cells. The proliferation of the cells were compared by cell counting and the cytotoxic activities to gastric carcinoma line BGC823 were determined by MTT.
     方法:脐血和正常人外周血各16例,经单个核细胞,以抗CD3mAb、重组人白细胞介素2和干扰素γ为诱导剂制备CIK细胞,直接活细胞计数法比较二者的细胞动力学效应,MTT法比较二者对胃癌细胞株BGC823的杀伤活性,透射电镜下观察肿瘤细胞的变化.
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  相似匹配句对
     Stability and Dynamic Effect of Hot Nucleus
     热核的稳定性和动力学效应
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     A DYNAMICAL MODEL DESCRIBING T CELL ACTIVATION
     T细胞活化的动力学模型
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     Dynamic Effect of Enterprise Production
     企业生产的动力学效应
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     the effector cell of RTE is T cells.
     RTE的效应细胞是T细胞 ;
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     Dynamical behaviour of a cellular neural network
     细胞神经网络的动力学行为
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  cytokinetic effect
These findings point to the S-phase blockade as the crucial cytokinetic effect of cisplatin preceding relevant growth reductions.
      


Objective To explore the method of primary myometrial cell culture to provide a valuable cell model for a series of research works. Methods Human mometrial cells were isolated by cutting and collagenase digestion, and incubated with DMEM medium and fetal bovine serum. The first passage cells were identified by anti-desmin and-α-action antibodies immunohistochemical staining. Results Primary cultivated human myometrial cells had high purity and viability. Conclusions Primary culture human myometrial cells...

Objective To explore the method of primary myometrial cell culture to provide a valuable cell model for a series of research works. Methods Human mometrial cells were isolated by cutting and collagenase digestion, and incubated with DMEM medium and fetal bovine serum. The first passage cells were identified by anti-desmin and-α-action antibodies immunohistochemical staining. Results Primary cultivated human myometrial cells had high purity and viability. Conclusions Primary culture human myometrial cells could obtained by cutting and collagenase digestin.

目的 掌握人子宫平滑肌细胞原代培养的方法 ,可以籍之开展一系列的研究工作。比如进行体外药物实验 ,研究细胞的增殖与遗传 ,了解平滑肌细胞的动力学效应等许多方面。而且该方法目前在国内未见成功报道。方法 用剪切胶原酶消化法分离人子宫平滑肌细胞。用DMEM和胎牛血清进行培养。传一代时用desmin和α -action单抗进行鉴定。结果 经实验该法成功率高 ,纯度高 ,细胞活力好。结论 用该法可以成功进行人子宫平滑肌细胞的原代培养

AIM: To compare the proliferation, cytotoxicity and the mechanism of CIK cells from two different tissues. METHODS: The peripheral blood and umbilical cord blood were harvested from 16 subjects. The lymphocytes were isolated by density gradient centrifugation and suspended in medium with CD3 mAb, rIL-2 and IFN- γ as inducing agents to prepare CIK cells. The proliferation of the cells were compared by cell counting and the cytotoxic activities to gastric carcinoma line BGC823 were determined by MTT. The changes...

AIM: To compare the proliferation, cytotoxicity and the mechanism of CIK cells from two different tissues. METHODS: The peripheral blood and umbilical cord blood were harvested from 16 subjects. The lymphocytes were isolated by density gradient centrifugation and suspended in medium with CD3 mAb, rIL-2 and IFN- γ as inducing agents to prepare CIK cells. The proliferation of the cells were compared by cell counting and the cytotoxic activities to gastric carcinoma line BGC823 were determined by MTT. The changes of tumor cells after induction with CIK cells were observed with transmission electron microscope. RESULTS: The proliferation speed and the anti-tumor activity of CIK cells originated from umbilical cord blood were significantly higher than those from peripheral blood (CMNC CIK vs MNC CIK, P <0.05). CIK cells from umbilical cord blood predominantly induced the necrosis of the tumor cells while those from peripheral blood mainly induced the apoptosis of the tumor cells. The two had significant difference. CONCLUSION: Compared with the peripheral blood CIK cells, umbilical cord blood CIK cells have the advantages of rapid proliferation speed and powerful cytotoxicity. The mechanism of cytotoxicity is different in peripheral blood CIK cells and in umbilical cord blood CIK cells.

目的:比较两种不同来源的CK细胞的体外扩增速度、杀伤活性和机制. 方法:脐血和正常人外周血各16例,经单个核细胞,以抗CD3mAb、重组人白细胞介素2和干扰素γ为诱导剂制备CIK细胞,直接活细胞计数法比较二者的细胞动力学效应,MTT法比较二者对胃癌细胞株BGC823的杀伤活性,透射电镜下观察肿瘤细胞的变化.结果:脐血CIK细胞的扩增速度、杀伤活性均显著优于外周血CIK细胞,CMNCCIKvsMNCCIK,P<0. 05;脐血CIK细胞以诱导肿瘤细胞坏死为主,外周血CIK细胞以诱导肿瘤细胞凋亡为主,二者的差异有统计学意义. 结论:脐血CIK细胞体外扩增快,杀伤活性强,对肿瘤细胞的作用优于外周血CIK细胞,不同来源的CIK细胞杀伤肿瘤细胞的机制不同.

 
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