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常规染色体
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  conventional chromosome
     Methods Using conventional chromosome aberraton analysis method, chromosome aberration frequence( CAF) of AT cells and GM cells exposed to 60CO γ-rays was observed at doses of 0, 1, 2, 3 and 4 Gg, compared with that of GM 0639(GM cells) from the skin of the normal subject. The difference of CAF between AT cells and GM cells was analyzed, and dose-response curves were established.
     方法 以源于正常人皮肤的成纤维细胞系GM0639(GM细胞)为对照,用常规染色体畸变分析法,在AT细胞和GM细咆经60COγ射线0、1、2、3、4 Gy照射后,观察比较AT细胞和GM细胞之间染色体畸变率(CAF)的差异,并分别进行曲线拟合。
短句来源
     Study on High Radiosensitivity of AT Cells by Conventional Chromosome Aberration Analysis Method
     常规染色体畸变分析法对AT细胞高辐射敏感性的研究
短句来源
     Calyculin A was added into the human blood samples irradiated by 60 Co γ rays at different doses(0, 0.25, 0.5, 1.0, 2.0, 3.0 Gy) after 46 h irradiation. Chromosome condensations induced by Calyculin A were observed and compared with conventional chromosome aberrations.
     采用不同剂量 (0、0 .2 5、0 .5、1.0、2 .0、3.0 Gy)的 60 Coγ射线照射离体人血 ,于照后 4 6 h加入Calyculin A,观察其诱导的染色体凝聚 ,并与常规染色体畸变法进行比较。
短句来源
     Liquid cell culture and conventional chromosome assay were used to evaluate the cytotoxic effect of IFN,HHT and Ara c on CML Ph + cell.
     以药物作用 2 4小时液体培养及常规染色体分析法研究 IFN、HHT及 Ara- c对 10例 CML 慢性期患者的骨髓中 Ph+ 细胞的生长影响。
短句来源
     The chromosome karyotype ofsika deersin Qing haiprovince werestudied by methodsof peripheralbloodlympho cytes culture and conventional chromosome analysis tech nique-Theresultsshowed:The diploid chromosomesnumberofsi ka deers was 2n = 64 ,65 ,66,68;
     采用外周学淋巴细胞培养方法和常规染色体分析技术,对青海地区的梅花鹿染色体进行了研究,结果显示:梅花鹿二倍体染色体数目Zn = 64 ,65,66 ,68;
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  routine chromosome
     FISH is more sensitive than the routine chromosome analyses in detecting low-level X chromosome monosomy .
     对低百分率染色体嵌合核型的检测,FISH技术在敏感性和特异性上优于常规染色体核型检查。
短句来源
     The results are as follows:1, The slides were prepared according to the routine chromosome preparation technique and were treated by DNase, RNase and NaCl, stained with silver nitrate. It was observated under the microscope and transmission electron microscope that: There is really a scaffold structure exited in common wheat chromosomes;
     1、对小麦根尖分生细胞进行常规染色体制片、DNA酶处理、RNA酶处理、NaCl处理、硝酸银染色,在光镜、电镜下对染色体骨架的形态结构特点等进行了观察和分析,结果表明:在小麦染色体中存在真实的骨架结构;
短句来源
     \ Methods\ By using routine chromosome preparation and Crocker′s methods,chromosome analysis and AgNOR were examined in 121 pleural effusion cases(46 malignant cases,75 benign cases).
     方法常规染色体制片和Crocker法,对46例恶性胸水、75例良性胸水患者进行染色体检查和AgNOR技术联合鉴别。
短句来源
  conventional chromosomal
     The karyoytpes and NORs of two native strains of A. sinensis cultivated in Minand Weiyuan counties in Gansu were studied by the conventional chromosomal preparationand AgNO_3 banding methods.
     应用常规染色体制片技术和AgNO_3分带技术对产于甘肃岷县和渭源县的两个栽培当归居群的染色体核型和NORs作了研究。
短句来源
     Peripheral blood from both groups was cultured at 37℃ for 48 h and 72 h, respectively, and then slides were prepared for conventional chromosomal aberration test and CBMN test. One hundred blood cells in metaphase and 1 000 binuclear lymphocytes in each sample were observed under microscope.
     2组对象的外周血在 37℃培养 48和 72h ,分别按常规染色体畸变试验和胞质阻断微核试验方法制片 ,每个样本分别观测 10 0个细胞间期和 10 0 0个双核淋巴细胞。
短句来源
     MATERIAL AND METHODS: Conventional chromosomal aberrations and G-banded kayrotype analysis were used.
     材料与方法:常规染色体畸变与G_显带核型分析。
短句来源
     Ph chromosome, a specific marker of CML, was assayed by conventional chromosomal analysis and fluorescence in situ hybridization, bcr/abl fusion gene was detected by reverse transcription-polymerase chain reaction.
     RT-PCR检测bcr/abl融合基因; 常规染色体核型分析及FISH检测细胞遗传学变化。
短句来源
  “常规染色体”译为未确定词的双语例句
     Results The conventional karyotypes of domestic dog were 2n=78.Female dogs were 78,XX and male dogs were 78,XY.
     结果 本地犬的常规染色体核型 2n =78,雌性为 78,XX ,雄性为 78,XY。
短句来源
     In one case, a very complex chromosmal translocation del(3), del(6)(q13 21), der(6)t(17;3;6), der(17)t(6;17) was characterized by chromosomal painting.
     此外,应用染色体涂抹技术检出了1例常规染色体分析未能确证的非常复杂的染色体易位,其同时存在del(3),del(6)(q1321),der(6)t(17;3;6),der(17)t(6;17),t(9;22)。
短句来源
     Result: Its specificity and sensitivity were 90.9%and 88.9% by routine method, and by FCM its were100%and 93.9%;
     结果 :常规染色体检查对恶性胸腔积液诊断的特异性为 90 .9% ,敏感性为 88.9% ; FCM法特异性为 1 0 0 % ,敏感性为 93.9% ;
短句来源
     Methods: G-banding and C-banding was performed for cytogenetic analysis,fluorescence in situ hybridization (FISH) technique was performed to analyze the case by using DYZ1 probe of Yq12 and DYZ3 probe of Yp11.1-q11.1, at the same time, Y chromosome microdeletion was also performed by PCR technique.
     方法应用实验室常规染色体标本制备方法进行G显带和C显带,并应用Yq12区DYZ1探针和Yp11.1-q11.1区DYZ3探针与病例的中期分裂相进行荧光原位杂交(fluorescence in situ hybridization,FISH),同时应用PCR技术对患者进行了Y染色体微缺失的检测。
短句来源
     With conventional G-banding analysis, 40% (6/15) patients were found with chromosomal abnormality involving CBF, including 5 of the 8 M2 patients with t (8;21) and 1 of the 2 M4EO patients with inv (16). No B-ALL cases were identified with t (12;21).
     (2)常规染色体G显带分析,15例患者中有6例伴有潜在累及CBF基因的染色体异常,其中8例AML-M2中5例伴t(8;21),2例AML-M4EO中1例伴inv(16),5例儿童B系急性淋巴细胞白血病(B-ALL)未检出相应的异常。
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  conventional chromosome
The slides processed for FISH can be used subsequently for conventional chromosome bandings.
      
In B-cell chronic lymphocytic leukemia (B-CLL) clonal chromosome aberrations are detected in approximately 40-50% of tumors when using conventional chromosome banding analysis.
      
This technique proved more efficient than a conventional chromosome-doubling method.
      
A simple method has been described for the visualization of chromosome cores with light microscopy in conventional chromosome preparations.
      
Positive controls consisted of conventional chromosome preparations from bone marrow aspirates demonstrating the Ph chromosome in all patients examined, and negative controls of paraffin sections of bone marrow biopsies from non-CML patients.
      
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  routine chromosome
Using a routine chromosome staining technique, the karyotype of white char (2n= 78) was shown to be identical to that of Dolly Varden.
      
G-banding of chromosomes promises to be the most valuable technique for routine chromosome analysis due to its inherent simplicity, sensitivity, and stability of the material obtained.
      
Routine chromosome analysis of two patients with severe mental retardation revealed two 45,D-,D-,t(DqDq)+karyotypes.
      
In a routine chromosome preparation 70-80% of chromosome sets are at the stage of metaphase.
      
An overall incidence of 7.1% constitutional abnormalities indicates that this criterion of selection may be advisable for routine chromosome analysis of infertile men.
      
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  conventional chromosomal
Two out of three deletions could not be detected by a conventional chromosomal analysis.
      


A cytogenetic study was made on 20 married couples,the wives having had spontane-ous abortion,stillbirth or deliveries of dead or abnormal babies.Four couples were foundto have chromosome aberration either in themselves or in their babies.In case 1,the cou-ple were under 30 years of age,Although their karyotypes were normal,one of their infantwas of 21 trisomy(47,xx,+21).In case 2,the wife was a 21/22 Robertson translocationcarrier with same karyotype as her mother(45,xx,-21,-22,+t(Zl_q22_q)/46,xx.In the 3rdcouple...

A cytogenetic study was made on 20 married couples,the wives having had spontane-ous abortion,stillbirth or deliveries of dead or abnormal babies.Four couples were foundto have chromosome aberration either in themselves or in their babies.In case 1,the cou-ple were under 30 years of age,Although their karyotypes were normal,one of their infantwas of 21 trisomy(47,xx,+21).In case 2,the wife was a 21/22 Robertson translocationcarrier with same karyotype as her mother(45,xx,-21,-22,+t(Zl_q22_q)/46,xx.In the 3rdcouple of first-cousin marriage.The chromosome 14 of the husband had broken.Hiswife,though with a normal karyotype,still gave birth to a baby that was with ichthyosis.He had a submetacentric chromosome that was similar to chromosome type C.In case 4,the wife had a long arm aberration of a chromosome type C of partial cell.She has hadstillbirth of babies for three times.The above detail may suggest that chromosome study with the purpose of genetic coun-selling is quite important in obstetrics clinic for eugenic purpose to improve the popula-tion's quality.

采用常规染色体制片和G 分带技术对20例(对)习惯性流产、早产、死产和分娩畸形儿的夫妇进行细胞遗传学检查,其中四例具有染色体异常。例一,夫妇年龄30岁以下,染色体均正常,生一先天愚型女孩,核型为(47,xx,+21)。例二,妻子和妻母均为罗伯逊21/22易位携带者,核型为[45,xx,-21,-22,+t(21q 22q)]/46,xx。例三,丈夫有D 组染色体断裂,生一男性死婴,患先天鱼鳞癣症,具有一条似C 组近中着丝点异常染色体。例四,妻子连续三次早死产,部分细胞具有一条C 组染色体长臂有增加的异常染色体。上述检查结果说明:在遗传咨询中,对有流产、早死产史的夫妇进行染色体检查是必要的。

In June 1978,a girl,19 years old,suffering from primary amenorrhoea came to theInternational Peace Maternity and Child Health Hospital of the China Welfare Institutein Shanghai for clinical examination.She was discovered to have a very large chromo-some in her peripheral blood lymphocytes.In March 1982,by using modern cytogenetictechniques such as G-handing,C-banding,late replicate X and high resolution G-handingdetection method,it was revealed that she had a karyotype with 45,X/46,X,idic(X)or 45,X/46,X,idic(X)(pter→cen→q27::q27→cen→pter).It...

In June 1978,a girl,19 years old,suffering from primary amenorrhoea came to theInternational Peace Maternity and Child Health Hospital of the China Welfare Institutein Shanghai for clinical examination.She was discovered to have a very large chromo-some in her peripheral blood lymphocytes.In March 1982,by using modern cytogenetictechniques such as G-handing,C-banding,late replicate X and high resolution G-handingdetection method,it was revealed that she had a karyotype with 45,X/46,X,idic(X)or 45,X/46,X,idic(X)(pter→cen→q27::q27→cen→pter).It is the first case discoveredin our country.The origin,identification and function of the marker chromosomeand its relationship to the patient's phenotype are discussed.

本文报道运用数种显带技术对一例原发性闭经患者进行了鉴定。该例患者经用常规染色体检查,见有一个很长的染色体,而用普通 G 带、普通 C 带、迟复制 X 染色体和高分辨 G 带技术检查,确证其核型中含有对称的双着丝粒 X 染色体,即 idic(X)核型。文中还就 idic(X)的形成和功能以及其与患者表现型间的关系作了探讨。

Karyotypes of the three Tilapia fishes (T.mossambica,T.nttotica,T.galilaeus) were investigated.The idiograms and relative idiograms were established on the basis of their karyotypes.All these three species of Tilapia have a diploid chromosome number of 44.Chromosome 1 is the longest,chromosome 2 is the second longest and the rest of the complement are all more or less of the same size.The chromosomes were classified into three groups: 4 submetacentric chromosome pairs,17 subtelocen-tric chromosome pairs and...

Karyotypes of the three Tilapia fishes (T.mossambica,T.nttotica,T.galilaeus) were investigated.The idiograms and relative idiograms were established on the basis of their karyotypes.All these three species of Tilapia have a diploid chromosome number of 44.Chromosome 1 is the longest,chromosome 2 is the second longest and the rest of the complement are all more or less of the same size.The chromosomes were classified into three groups: 4 submetacentric chromosome pairs,17 subtelocen-tric chromosome pairs and 1 telocentric pair.The karyotypes of these Tilapia fishes are basically similar in morphology.It is difficult to distinguish these very closely related Tilapia species by the conventional technique.The variation of karyotypes and their evolution in Tilapia are disscused.

本文对莫桑比克罗非鱼、尼罗罗非鱼、加俐俐罗非鱼的染色体组型进行了分析研究,并绘制了它们各自的核型模式图。所有这3种罗非鱼的二倍体染色体数目都是44,即2n=44。第1对染色体最长,第2对次之,其余20对均为大小差不多的染色体。它们的核型可分为3组,SM组4对,ST组17对,T组1对。3种罗非鱼的染色体组型形态基本相似,用常规染色体染色的方法难以区分。本文还讨论了罗非鱼的核型与进化问题。

 
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