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   癌胚抗原基因 的翻译结果: 查询用时:0.036秒
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癌胚抗原基因
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  carcinoembryonic antigen gene
     Determination of carcinoembryonic antigen gene transfection dendritic cell expression with RT-PCR
     RT-PCR检测癌胚抗原基因转染树突状细胞的表达
短句来源
     The composing of carcinoembryonic antigen gene vaccination
     人癌胚抗原基因疫苗的构建
  cea gene
     The construction of recombinant CEA gene vaccine and its immune effect
     癌胚抗原基因重组疫苗的构建及免疫效果观察
短句来源
  “癌胚抗原基因”译为未确定词的双语例句
     AIM: To investigate the inhibition of dendritic cells(DCs)(pcDNA3-hCEA) inoculation on MGC803 in nude mice.
     目的:探讨转染癌胚抗原基因pcDNA3-hCEA的人树突状细胞(DC)对胃癌细胞MGC803裸鼠移植瘤生长的影响.
短句来源
     The Expression of Dendritic Cell Transfected with Plasmid Carcinoembryonic Antigen
     转染癌胚抗原基因的人树突状细胞疫苗表达的研究
短句来源
     We have study on the possibility of using the CEA promoter in specific cancer gene therapy.
     本文研究了癌胚抗原基因(Carcinoembryonic Antigen,CEA)启动子用于靶向性基因治疗的可能性。
短句来源
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     The composing of carcinoembryonic antigen gene vaccination
     人癌胚抗原基因疫苗的构建
     Preparation of carcinoembryonic antigen positive tumor gene vaccine capsules for oral use
     癌胚抗原肿瘤基因疫苗胶囊的研制
短句来源
     Genetic Information Carrier Gene
     基因简史
短句来源
     THE ISOLATION AND PURIFICATION OF CARCINOEMBRYONIC ANTIGEN
     癌胚抗原的提取和纯化
短句来源
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  carcinoembryonic antigen gene
Characterization of murine carcinoembryonic antigen gene family members
      
The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomalin situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3.
      
A mouse carcinoembryonic antigen gene family member is a calcium-dependent cell adhesion molecule.
      
  cea gene
According to the fact that CEA gene expressed only in lung adenocarcinoma and not in normal lung cells, a retroviral vector (pCEAMR) was constructed which carried the CEA promoter coupled to MDRl ribozyme gene.
      
These results show the possibility of HSV-TK gene-drug therapy using the tumor-specific promoter of CEA gene against CEA-producing lung cancers which was usually refractory to conventional chemotherapy.
      
We investigate the possibility of targeted gene therapy using promoter of carcinoembryonic antigen (CEA) gene.
      
The glycoprotein of the carcinoembryonic antigen (CEA) gene family expressed on epithelial keratinocytes in viral warts
      
It is a member of the carcinoembryonic antigen (CEA) gene family, which is a subfamily of the immunoglobulin gene superfamily.
      
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Objective To produce a bio engineered singlechain antibody to carcinoembryonic antigen that can be used in tumor immunodiagnosis and therapy. Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface. By three panning rounds, we have obtained a single chain antibody that was specific for carcinoembryonic antigen, referred as CL 3 scFv. Results ELISA and immunohistochemistry showed that the antibody could be bound to carcinoembryonic...

Objective To produce a bio engineered singlechain antibody to carcinoembryonic antigen that can be used in tumor immunodiagnosis and therapy. Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface. By three panning rounds, we have obtained a single chain antibody that was specific for carcinoembryonic antigen, referred as CL 3 scFv. Results ELISA and immunohistochemistry showed that the antibody could be bound to carcinoembryonic antigen as well as to tumor cells in colon and gastric carcinomas but not normal cells. Conclusion The antibody CL 3 scFv will be a reagent for the diagnosis and therapy of gastrointestinal cancer.

目的 研制识别癌胚抗原的基因工程单链抗体,使其能够应用于肿瘤的免疫诊断和治疗。方法 利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。通过免疫筛选获得癌胚抗原特异单链抗体CL3scFv 。结果 ELISA 和免疫组化均显示抗体CL3scFv 能够结合CEA,而不与正常胃肠道组织起反应。结论  该抗体将成为胃肠道恶性肿瘤辅助诊断和治疗的有效试剂。

Objective:1)To construct two carcinoembryonic antigen(CEA)recombinant ex-pression plasmids,in which the full length complementary DNA(cDNA)for human CEA is under the con trol of different promoters;2)To detect the carcinoembryonic antigen expressed in mice tongues by direct intramuscular injec tion of these CEA recombinant expression plasmids.Methods:At first,molecular cloning techniques were applied to construct two recombinant expression plas-mids,in which the human CEA cDNA was driven by CMV promoter...

Objective:1)To construct two carcinoembryonic antigen(CEA)recombinant ex-pression plasmids,in which the full length complementary DNA(cDNA)for human CEA is under the con trol of different promoters;2)To detect the carcinoembryonic antigen expressed in mice tongues by direct intramuscular injec tion of these CEA recombinant expression plasmids.Methods:At first,molecular cloning techniques were applied to construct two recombinant expression plas-mids,in which the human CEA cDNA was driven by CMV promoter /enhancer(pCEA)or LTR(pLCEASN),respectively.Groups of mice(n=4),which were in tra muscular injected with0.75%bupivacaine7days ago,were in tra muscular injected with pCEA?pLCEASN?pcDNA3or pLXSN,100μg does week ly for three injections,respectively.The sera from tailer vein were harvested at proinjection and each2days postin jection for radio-immunology assay.All mice were sacrificed2days after the last injection,and the tongue?brain?liver?kidney?spleen?heart and quadricep muscle were re moved for CEA im munohisto chemical staining(ABC method).Results:1)Two car-cinoem bry on ic anti gen re combinant expression plasmids were con structed and identified;2)ABC staining for CEA were positive in sections of the tongue injected with plasmid DNA of pCEA?pLCEASN except pcDNA3and pLXSN,but negative in the brain?liver?kidney?spleen?heart and quadricep muscle;3)The CEA con centrations were under the detectable CEA level(<5ng /ml)by ra dio-im munolo gy assay in all sera samples.Con clusion:Human CEA cDNA can be ex-pressed effectively in situ after injection of these CEA re combi nant expression plasmids,in which the human CEA cD NA is driv en by different promoters.

构建含不同启动子的人癌胚抗原重组表达质粒,观察肌肉注射该重组质粒后人癌胚抗原基因在小鼠舌肌中的表达情况。方法:将7天前肌肉注射0.75%布比卡因的小鼠分成4组,每组4只,分别将含不同启动子的重组质粒pCEA、pLCEASN及阴性对照质粒pcDNA3、pLXSN注射于小鼠的舌肌内,每周1次,每次100μg,共3次;注射前及每次注射后2天,取尾静脉血用放免法测其癌胚抗原的含量,最后1次注射2天后,处死小鼠并取其舌、脑、肝、脾、肾、心脏及股四头肌用免疫组化法(ABC法)检测癌胚抗原的表达。结果:构建并鉴定了两种新的癌胚抗原重组表达质粒;免疫组织化学法检测显示:癌胚抗原ABC染色仅在用人癌胚抗原重组表达质粒注射过的舌肌切片中阳性,而在脑、肝、脾、肾、心脏及股四头肌的切片中均阴性;放免法检测显示:所有血液样本中癌胚抗原的浓度均未达检测值(<5ng/ml)。结论:含不同启动子的癌胚抗原重组表达质粒可在注射部位有效地表达人癌胚抗原。

AIM: To investigate the inhibition of dendritic cells(DCs)(pcDNA3-hCEA) inoculation on MGC803 in nude mice.METHODS: DCs were generated from human fresh peripheral blood in the presence of rhGM-CSF and rhIL-4 and DC vaccine was prepared by transfection with pcDNA3-hCEA using lipofectine.CEA mRNA expressing in DCs(pcDNA3-hCEA) was confirmed by RT-PCR and specific cytotoxic T lymphocytes(CTL) was induced in vitro.The inhibition of the DC(pcDNA3-hCEA) inoculating on MGC803 in nude mice was observed.RESULTS: CEA...

AIM: To investigate the inhibition of dendritic cells(DCs)(pcDNA3-hCEA) inoculation on MGC803 in nude mice.METHODS: DCs were generated from human fresh peripheral blood in the presence of rhGM-CSF and rhIL-4 and DC vaccine was prepared by transfection with pcDNA3-hCEA using lipofectine.CEA mRNA expressing in DCs(pcDNA3-hCEA) was confirmed by RT-PCR and specific cytotoxic T lymphocytes(CTL) was induced in vitro.The inhibition of the DC(pcDNA3-hCEA) inoculating on MGC803 in nude mice was observed.RESULTS: CEA mRNA of DCs(pcDNA3-hCEA) could be detected at 588 bp with a pair of specific primers by RT-PCR and the specific CTL(%) at effect-target rate 10∶1,20∶1,40∶1 were respectively 19.4±3.8,24.7±3.7,38.1±5.4.DCs(pcDNA3-hCEA) effectively inhibited the growth of MGC803.The median of tumor genesis time in the experiment group was longer than that of control(10 d vs 6 d,n=5) and the growth speed of tumor inoculating on DCs(pcDNA3-hCEA) in nude mice was slower than that in control group.The size of tumor inoculating on DCs(pcDNA3-hCEA) in nude mice was less than that in control group [(0.24±0.10) cm~3 vs(1.36±0.42) cm~3,n=5,P<0.05].CONCLUSION: The human dendritic cells transfected with pcDNA3-hCEA can inhibit MGC803 cells in nude mice.

目的:探讨转染癌胚抗原基因pcDNA3-hCEA的人树突状细胞(DC)对胃癌细胞MGC803裸鼠移植瘤生长的影响.方法:采用细胞因子rhIL-4和rhGM-CSF诱导培养法制备人外周血DC,并用L ipofectine向人DC转染pcDNA3-hCEA.RT-PCR检测转染人CEA真核表达质粒的表达.使用DC(pcDNA3-hCEA)疫苗体外诱导人T淋巴细胞靶向性杀伤反应,并在体内实验中观察DC(pcDNA3-hCEA)对MGC803在裸鼠上致瘤性的影响.结果:针对特定引物DC(pcDNA3-hCEA)在588 bp处有CEA片段的表达;DC(pcDNA3-hCEA)能够诱导的人T淋巴细胞靶向性杀伤活性(%),在效靶比分别为10∶1,20∶1,40∶1时的结果为19.4±3.8,24.7±3.7,38.1±5.4;DC(pcDNA3-hCEA)能显著抑制MGC803的生长,其肿瘤生成日期较对照组延长(中位数10 dvs6 d,n=5),瘤体生长速度较对照组缓慢,d 23瘤体体积明显小于对照组[(0.24±0.10)cm3vs(0.65±0.17)cm3,(1.36±0.42)cm3,n=5,P<0...

目的:探讨转染癌胚抗原基因pcDNA3-hCEA的人树突状细胞(DC)对胃癌细胞MGC803裸鼠移植瘤生长的影响.方法:采用细胞因子rhIL-4和rhGM-CSF诱导培养法制备人外周血DC,并用L ipofectine向人DC转染pcDNA3-hCEA.RT-PCR检测转染人CEA真核表达质粒的表达.使用DC(pcDNA3-hCEA)疫苗体外诱导人T淋巴细胞靶向性杀伤反应,并在体内实验中观察DC(pcDNA3-hCEA)对MGC803在裸鼠上致瘤性的影响.结果:针对特定引物DC(pcDNA3-hCEA)在588 bp处有CEA片段的表达;DC(pcDNA3-hCEA)能够诱导的人T淋巴细胞靶向性杀伤活性(%),在效靶比分别为10∶1,20∶1,40∶1时的结果为19.4±3.8,24.7±3.7,38.1±5.4;DC(pcDNA3-hCEA)能显著抑制MGC803的生长,其肿瘤生成日期较对照组延长(中位数10 dvs6 d,n=5),瘤体生长速度较对照组缓慢,d 23瘤体体积明显小于对照组[(0.24±0.10)cm3vs(0.65±0.17)cm3,(1.36±0.42)cm3,n=5,P<0.05].结论:转染pcDNA3-hCEA的人DC能有效抑制MGC803在裸鼠体内的生长.

 
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