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   早期凋亡 的翻译结果: 查询用时:0.988秒
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早期凋亡     
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  early apoptosis
     Results After treated with octreotide at a concentration of 1×10~ -5 mol/L , the early apoptosis rates (14.2 ± 2.6) % and the apoptosis indexes (18.8±3.3)% were significantly higher than the control group(1.2 ± 0.1)% (P< 0.05) and (3.6 ± 0.9) % (P<0.01).
     结果1×10-5mol/L奥曲肽使HepG2细胞早期凋亡阳性率和凋亡指数(AI)增加至(14.2±2.6)%和(18.8±3.3)%,与对照组(1.2±0.1)%(P<0.05)和(3.6±0.9)%(P<0.01)比较差异显著;
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     The early apoptosis rates of the adriamycin /etoposide + IFNγ+TRAIL groups [( 17.9±3.6)%, (14.8±3.3)%] were higher than that of the IFNγ+TRAIL group [(3.9± 1.2)% ](F=26.233, P< 0.01).
     阿霉素/依托泊苷+IFNγ+TRAIL组早期凋亡率为:(17.9±3.6)%、(14.8±3.3)%,与IFNγ+TRAIL组(3.9±1.2)%比较,差异有显著性(F=26.233,P<0.01)。
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     (3) The percentage of SKOV3/siRNA in early apoptosis was (10.500±0.250)%, while the percentage of SKOV3/siRNA-negative was (0.340±0.010)% (P<0.01).
     (3)SKOV3/siRNA细胞的早期凋亡率为(10·500±0·250)%,而SKOV3/siRNA-negative细胞为(0·340±0·010)%,两者比较,差异有统计学意义(P<0·01)。
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     300 μg/mL of GS affected on SGC-7901 cells for 24 h, cells appeared the modality of early apoptosis;
     300μg/mL的GS作用SGC-7901细胞24h后,细胞出现早期凋亡的形态;
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     After treatment with PDS containing 4.25% glucose for 3 h, the proliferation inhibition rate of the RPMCs reached 44.12%, the percentage of cells at the G0/G1 stage amounted to 71.95% and the early apoptosis rate reached 23.59%, which was several times higher than that of the negative control and 1.5% glucose/PDS groups, and also higher than that of 4.25% mannitol/PDS groups.
     含4.25%葡萄糖的腹透液刺激3h后,细胞增殖抑制率达44.12%,G0/G1期细胞占71.95%,早期凋亡率达23.59%,远远高于不含糖的阴性对照组和含1.5%糖腹透液组(P<0.001),也高于4.25%甘露醇组(P<0.05)。
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  early apoptotic
     early apoptotic cell percentage: Group A(77.8±4.3)% and Group B(17.6±(5.9))%;
     早期凋亡细胞百分比:A组(77.8±4.3)%,B组(17.6±5.9)%;
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     Results:The percentage of the early apoptotic lymphocytes in the peripheral blood of active SLE patients was significantly higher(12 61±6 32, n =22)than that of the inactive SLE patients (4 47±3 39, n =8, P <0 01),and of the normal healthy donors (5 13±3 37, n =11, P <0 001),but it was not correlated to SLAM Score.
     结果:SLE活动期患者外周血淋巴细胞早期凋亡率(1261±632,n=22)明显高于稳定期患者(447±339,n=8,P<001)及正常人(513±337,n=11,P<0001),但与SLAM评分无相关。
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     RESULTS: 6 h after treated with 10 μmol/L CPT,the rate of early apoptotic cells(22.59±1.04)% had significantly difference compared with control group(3.93±0.73)%(P<0.01).
     L-1CPT诱导下,6 h时Jurkat细胞早期凋亡的细胞比率(22.59±1.04)%显著高于对照组(3.93±0.73)%(P<0.01)。
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     The early apoptotic cells presented green fluorescent label which could be observed in the 20 μmol/L Aβ25-35 group incubated for only 1 d;
     Aβ25-3520μmol/L组孵育1d后,即可观察到处于早期凋亡的细胞呈现绿色荧光标记;
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     (5) When colo-16 cells were incubated with 50μg/ml iso-CPT for 48 hours ,the early apoptotic rate was 4.45%.
     (5)AnnexinⅤ-PI法检测50μg/ml喜树异碱作用48小时colo-16细胞早期凋亡比例为4.45%。
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  early stage apoptosis
     Early stage apoptosis examination:when the cells were treated with 2 0,1 5,3 0 g/L matrine for 6,12,12 h,their percentages of apoptosis cells were 7 7,8 6,15 7% respectively.
     细胞早期凋亡检测 :2 0 ,1 5 ,3 0g/L苦参碱分别作用 6h、12h、12h ,凋亡细胞分别达 7 7,8 6 ,15 7% ;
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     Results: Early stage apoptosis examination: When the cells were treated with 50%,30%,20%,10%,5% cigarette smoke extract for 24 h,their percentages of apoptosis cells were 0,31.7%,11.9%,1.6%,0.3% respectively. At the same time,the percentages of necrosis cells were 100%,34.2%,6.2%,1.1%,0.8%.
     结果:50%、30%、20%、10%及5%浓度的香烟烟雾提取物处理细胞24 h后,经细胞早期凋亡检测,细胞凋亡率分别达到0、31.7%、11.9%、1.6%、0.3%,细胞坏死率分别为100%、34.2%、6.2%、1.1%、0.8%。
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     Incubated with THW 4 (10-100 μg/L) for 56 hrs, VSMC mainly appeared early stage apoptosis and the percentage of apoptosis was found to raise along with the increase of the THW 4 concentration.
     VSMC在含 10~ 10 0 μg/LTHW 4的培养液中孵育 5 6h ,可检测到细胞早期凋亡 ,且凋亡率随THW 4浓度增加而升高 ;
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     Objective: To establish a primary culture method for newborn rat hippocampal cells, and investigate the early stage apoptosis and death of cultured nerve cells using Annexin V-PI staining protocol.
     目的 建立新生大鼠海马神经细胞原代培养的方法,并利用Annexin V-PI双染法流式细胞仪检测培养过程中细胞早期凋亡和死亡情况。
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     DNA break was detected with TUNEL in situ assay, the apoptosis peaks were detected with flow cytometry, the early stage apoptosis was detected with Annexin V FITC/PI double labelled assay, the expression of apoptosis associated genes wp53, bcl 2, c myc, bax and Fas were detected with immunohistochemical technique and in situ hybridization , all results were quantitatively analyzed with high fidelity colored picture dissector.
     AnnexinV FITC/PI双标记检测早期凋亡 ; 免疫组化和原位杂交法检测wp5 3 ,bcl 2 ,bax ,c myc ,Fas等凋亡相关基因的表达 ,采用真彩色图像分析仪定量检测。
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  acute apoptosis
     ObjectiveTo observe the antagonistic effect of neurotropin to the acute apoptosis in nigral dopaminergic neurons following a 6-hydroxydopamine lesion in Sprague-Dawley (SD) rats.
     【目的】探讨神经托平(neurotropin)对6-OHDA诱导的SD(Sprague-Dawley)大鼠黑质多巴胺能神经元极早期凋亡的拮抗作用。
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      early apoptosis
    Annexin V/PI bivariate flow cytometric analysis further revealed that the five extracts significantly induced early apoptosis in HL-60 cells.
          
    Cell cycle analysis, early apoptosis analysis with double staining with Annexin V-FITC and PI, and active caspase-3 analysis with the staining of FITC-conjugated monoclonal rabbit anti-active caspase-3 antibody were made by flow cytometer.
          
    This sensitive parameter for early apoptosis detected different cell death kinetics, as well as varying sensitivity to the inhibitory effect of SOD in monocytes and lymphocytes.
          
    These methods entail detecting surface-exposed phosphatidylserine (early apoptosis), and measuring PMN chromatin condensation and DNA fragmentation (late apoptosis).
          
    Staining for the early apoptosis markers cleaved caspase 3 and cytochrome C did not reveal any significant differences between treated and control rats.
          
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      early apoptotic
    Bcl-2 inhibits early apoptotic events and reveals post-mitotic multinucleation without affecting cell cycle arrest in human epit
          
    Moreover, phosphatidylserine flipping on cell membrane could be detected with 3, 6 and 12?h cordycepin treatment, which indicated an early apoptotic phenomenon.
          
    The chromatin ultrastructure of early apoptotic cells initially retained the supranucleosomal order of packaging which characterises control cells, whereas the dense chromatin of late apoptotic cells possessed the degraded structure.
          
    Absorption spectra of the toluidine blue-stained early apoptotic cell chromatin revealed a metachromatic shift, indicating a change of DNA conformation and polymerisation of the dye.
          
    The results suggest that the enhanced affinity of the chromatin of early apoptotic cells for cationic dyes is associated with conformational relaxation rather than degradation of DNA.
          
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      acute apoptosis
    It is concluded that EAF with an attenuated p53 response can be induced by low doses of genotoxic compounds, not giving rise to acute apoptosis or necrosis.
          


    Objective:To detect the forms and amount of murine thymocytes through cell cultivation.Methods:Murine thymocytes cultured at a temperature of 4 ℃ and stained with AO/EB were observed under a fluoroscope at different time.Results:Under fiuoroscope,early apoptosis cells were seen to emit green or dark green fluoroscopes,with nuclear morphological changes.Late apoptosis cells,however,sent out orange-colored fluorescence,with formation of apoptosis bodies.Conclusions:Both qualitative and quantitative analysis of...

    Objective:To detect the forms and amount of murine thymocytes through cell cultivation.Methods:Murine thymocytes cultured at a temperature of 4 ℃ and stained with AO/EB were observed under a fluoroscope at different time.Results:Under fiuoroscope,early apoptosis cells were seen to emit green or dark green fluoroscopes,with nuclear morphological changes.Late apoptosis cells,however,sent out orange-colored fluorescence,with formation of apoptosis bodies.Conclusions:Both qualitative and quantitative analysis of the apoptosis can be made with the AO/EB double fluorescent staining technique.

    目的:检测细胞培养中凋亡细胞的形态及数量。方法:取4℃环境下不同时间培养的小鼠胸腺细胞,用AO/EB染色后荧光镜观察。结果:荧光镜下早期凋亡细胞发绿色或暗绿色荧光,伴有核结构的变化,晚期凋亡细胞发橙红色荧光并伴有凋亡小体的形成。结论:AO和EB双重荧光染色后,荧光镜检在细胞凋亡检测中既可定性,又可定量。

    To observe the morphology and amount of apoptosis cells, the authors detected murine thy-mocytes prepared at 4 C with different lengths of time by light microscope or fluoroscope after AO + EB staining. Under light microscope apoptosis bodies were found, while under fluoroscope early apoptosis cells accompanied with nuclear morphologic changes emitting green or dark green fluorescence and late apoptosis cells scintillating reddish orange fluorescence often had apoptosis bodies. The results showed that apoptosis...

    To observe the morphology and amount of apoptosis cells, the authors detected murine thy-mocytes prepared at 4 C with different lengths of time by light microscope or fluoroscope after AO + EB staining. Under light microscope apoptosis bodies were found, while under fluoroscope early apoptosis cells accompanied with nuclear morphologic changes emitting green or dark green fluorescence and late apoptosis cells scintillating reddish orange fluorescence often had apoptosis bodies. The results showed that apoptosis was identified only when the typical change of nuclear morphology appeared under light microscope and that both qualitative and quantitative analysis for determining apoptosis could be done by the double fluorescence staining technique.

    为观察细胞培养中细胞凋亡的形态及数量,取4℃环境下不同时间培养的小鼠胸腺细胞,用光镜或吖啶橙(AO),溴化乙锭(EB)染色后荧光镜观察。光镜下可见凋亡小体,荧光镜下早期凋亡细胞发绿色或暗绿色荧光,伴有核结构的变化,而晚期凋亡细胞发橙红色荧光并有凋亡小体的形成。结果表明:光镜下需出现典型核形态变化时凋亡才能确认,AO和EB双重荧光染色后荧光镜检在细胞凋亡检测中既可定性,又可定量。

    Objective:To determine whether the apoptosis in human SLE patients is abnormal by examining the early apoptotic lymphocytes in their peripheral blood.Methods:Early apoptotic cells were made salient by Annexin V FITC staining method and detected with flow cytometry.Results:The percentage of the early apoptotic lymphocytes in the peripheral blood of active SLE patients was significantly higher(12 61±6 32, n =22)than that of the inactive SLE patients (4 47±3 39, n =8, P <0 01),and of the normal healthy...

    Objective:To determine whether the apoptosis in human SLE patients is abnormal by examining the early apoptotic lymphocytes in their peripheral blood.Methods:Early apoptotic cells were made salient by Annexin V FITC staining method and detected with flow cytometry.Results:The percentage of the early apoptotic lymphocytes in the peripheral blood of active SLE patients was significantly higher(12 61±6 32, n =22)than that of the inactive SLE patients (4 47±3 39, n =8, P <0 01),and of the normal healthy donors (5 13±3 37, n =11, P <0 001),but it was not correlated to SLAM Score. Of the active SLE patients,there was no marked difference between the incipient and the treated (with corticosteroid or immunosuppressive therapy).Besides,no difference was found between inactive SLE and normal healthy donors. Conclusion:The early apoptotic lymphocytes increase significantly in the peripheral blood of human SLE patients,which may contribute to the pathogenesis of SLE by releasing an excessive amount of apoptotic DNA fragments.

    目的:为了观察SLE患者体内细胞凋亡情况,对其外周血淋巴细胞早期凋亡率进行了检测。方法:应用最新的AnnexinV检测试剂盒及流式细胞仪检测早期凋亡细胞。结果:SLE活动期患者外周血淋巴细胞早期凋亡率(1261±632,n=22)明显高于稳定期患者(447±339,n=8,P<001)及正常人(513±337,n=11,P<0001),但与SLAM评分无相关。活动期患者初发病组(1160±846,n=8)与已用激素、免疫抑制剂治疗组(1311±513,n=14)相比无差异。稳定期患者与正常人相比无差异。结论:SLE患者外周血淋巴细胞凋亡率明显升高,其可能的致病机制为通过释放过量核酸抗原而诱导自身免疫病发生

     
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