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q热
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  q fever
     Experimental Study of Enzyme-linked Immunosorbent Assay (ELISA) for Detection of Q Fever Antibodies
     酶联免疫吸附试验(ELISA)检测Q热抗体的实验研究
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     Of the 788 serum samples of healthy subjects, positive rate is 39.21% (309) for typhus, 1.65 % (13) for tsutsugamushi disease, and 4.19 % (33) for Q fever.
     788份健康人血清中斑疹伤寒阳性者为309份(39.21%),恙虫病阳性者13份(1.65%),Q热阳性者33份(4.19%)。 x~2检验表明。
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     In experiment examination, purely Q fever infection patients have a higher blood platelets counts(p<0.05).
     在实验室检查方面,单纯Q热柯克斯体感染患者血小板计数明显较肺炎链球菌感染患者高(p<0.05);
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     Immunohistochemical staining and in situ hybridization were applied to study the pathogenesis of Q fever and detect the antigen and DNA of Coxiella burnetii in BALB/c mice introperitoneally inoculated with Coxiella bur-netii.
     腹腔注射Q热立克次体悬液感染BALB/c小鼠,发病后解剖,取主要组织脏器,应用免疫组化和原位杂交检测感染小鼠体内Q热立克次体的抗原分布和DNA在靶细胞中的表达,探讨Q热病变规律及致病机理。
短句来源
     Collect 500 CAP cases from 12 territory hospital from china from November 2001 to June 2002. Use ELISA kit to detect each patient’s content of Q fever Coxiella burnetii specific IgG antibody in acute stage and restoration period, and statistical analysis the Q fever patients’ sex, age and other information.
     收集2001年11月至2002年6月从全国选择出的12所三甲医院CAP患者共500例。 使用ELISA试剂盒分别检测患者急性期及恢复期Q热柯克斯体特异性IgG抗体含量,对检测出的Q热柯克斯体的患者的性别、年龄等情况进行统计分析。
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     Detection of Coxiella burnetii by semi-nested PCR and DNA probe
     半套式PCR和DNA探针技术检测Q热立克次体
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     ANALYSIS OF THE16S-23S INTERGENIC SPACER REGION OF THE CHINESE COXIELLA BURNETII ISOLATES
     Q热立克次体中国株的16S-23SrDNA间区序列分析
短句来源
     The inhibitory effect of C1q on TNF-αproduction was abrogated when the C1q was heat inactivated or the C1q was incubated with U937 cell in presence of anti-C1q F(ab')2. An inhibition of TNF-α production by the anti-C1qR monoclonal antibody E8 was also demonstrated.
     将C1q热灭活或加入抗人C1qF(ab')2,C1q的抑制作用即消失,证实该作用是C1q特异的。 此外,抗人C1qRmAbE8能模拟C1q诱导类似的抑制效应。
短句来源
     DETECTION OF COXIELLA BURNETTI BY NESTED PCR AND DNA PROBE
     套式PCR和DNA探针技术检测Q热立克次体
短句来源
     Analysis of the 16S-23S intergenic spacer region(ISR) of Coxiella burnetii Chinese isolates with PCR-SSCP
     PCR-SSCP分析Q热立克次体中国分离株16S-23SrDNA基因间区
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  q fever
Q fever, an endemic disease in this area, should be also ruled out before the diagnosis of NANB hepatitis is established.
      
Q fever in Europe: Current aspects of aetiology, epidemiology, human infection, diagnosis and therapy
      
Q fever meningoencephalitis associated with bilateral abducens nerve paralysis, bilateral optic neuritis and abnormal cerebrospi
      
Q fever is an zoonosis caused byCoxiella burnetii, the clinical features of which are often non-specific and self-limited.
      
We describe a patient who developed reversible bilateral abducens nerve paralysis and bilateral optic neuritis in the course of acute Q fever meningoencephalitis.
      
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Biological properties of Q fever rickettsia "Li" strain ( Chinese), were studied after its isolation from a patient in the Inner Mongolia Autonomous Region. This strain could multiply well and give a richer growth after the 5 th passage in yolksac. It could also grow well in vacuoles of cells in cultures of mouse embryo and FL cells. Results suggested that this strain was similar to the Grita strain in developing immunity in both I and I phases in infec-tivity protection test with guinea pigs, mice or Chinese...

Biological properties of Q fever rickettsia "Li" strain ( Chinese), were studied after its isolation from a patient in the Inner Mongolia Autonomous Region. This strain could multiply well and give a richer growth after the 5 th passage in yolksac. It could also grow well in vacuoles of cells in cultures of mouse embryo and FL cells. Results suggested that this strain was similar to the Grita strain in developing immunity in both I and I phases in infec-tivity protection test with guinea pigs, mice or Chinese hamsters. Serolog-ical tests with convalescent g.p. sera showed a quick response of CF antibody to the phase I antigen. Only one week was required for the appearnce of this antibody and 4 weeks for reaching the peak. However, 6 weeks were required for the appearance of the phase I antibody. Results suggested that this strain was in alignment with the phase variations of Q fever rickettsia.

本文报告我国分离的Q热立克次体李株生物学性状的研究结果。通过一系列试验证明李株为一株典型的Q热立克次体。

Immunofluorescent technique with slide solid phase antiboby was used for the detection of Coxiella burnetii. The experimental conditions and the specificity of this new technique were compared with those of the conventional immunoiluorescent technique. Both techniques were used to examine the specimens of infected Orni-thodoros moubada and the results were also compared. It was found that when the new technique was used to detect C. burnetii, the non-specific fraction of the fluorescence in the specimen was...

Immunofluorescent technique with slide solid phase antiboby was used for the detection of Coxiella burnetii. The experimental conditions and the specificity of this new technique were compared with those of the conventional immunoiluorescent technique. Both techniques were used to examine the specimens of infected Orni-thodoros moubada and the results were also compared. It was found that when the new technique was used to detect C. burnetii, the non-specific fraction of the fluorescence in the specimen was significantly decreased and the sensitivity to the extracellular antigen was not affected.

本实验对用玻片固相抗体免疫荧光检查Q热立克次体进行了实验条件、特异性试验及感染蜱标本的初步研究。与常规免疫荧光技术比较,在检查Q热立克次体的标本中其非特异性荧光显著降低,而检查细胞外的抗原其敏感性也较好。

Ornithodoros moubata were expermentally infected with Coxiella burneti through the haemocoel. The dilutions of the infected chick yolk sacs were prepared in series of 10-fold from 10-1-10-12. Each of these dilutions was inoculated into each group of. 5 ticks. The haemolymph samples from these ticks were taken on the 35th day after inoculation. In the haematocyte test, the microorganisms in the body were examined both with Gimenez stain and immunofluoreseence technique.The results of the experiments showed that...

Ornithodoros moubata were expermentally infected with Coxiella burneti through the haemocoel. The dilutions of the infected chick yolk sacs were prepared in series of 10-fold from 10-1-10-12. Each of these dilutions was inoculated into each group of. 5 ticks. The haemolymph samples from these ticks were taken on the 35th day after inoculation. In the haematocyte test, the microorganisms in the body were examined both with Gimenez stain and immunofluoreseence technique.The results of the experiments showed that in large-doses such as 10-1-10-5, the microorganisms in the haemolymph samples were found similar by these two techniques. However, in lower-doses as 10-7, 10-8 and 10-10, they were positive by immunofluorescence technique, but negative by Gimenez stain.The positive percentage in haematocyte test with Gimenez stain was 40.68% and by immunofluorescence technique was 48.28%. Therefore, the immunofluorescence technique is more sensitive than the Gimenez stain in the detection of C. burneti in haematocyte test.12 groups of guinea pigs were inoculated with the dilutions of the suspension of infected ticks for 48 days, the blood samples for complement-fixation test were taken by cardiac puncture on the 26th day after inoculation. All of the guinea pigs inoculated were complement-fixation test positive, but the incubation period and the duration of fever among these guinea pigs suffered from C. burneti infections varied in these groups of different dilutions: The length of incubation period was 1 day (on average) in groups of dilutions of 10-1-10-3; 2.6 days (on average) in dilutions of 10-4-10-6, and extended to 4.6 days (on average) in dilutions of 10-7-10-12. The duration of fever were for 5 days (on average) in groups of dilutions of 10-1-10-3; 4.6 days (on average) in dilutions of 10-4- 10-6, and shorten to two days (on average) in the other dilutions of 10-7-10-12.It is suggested that the incubation period and durations of fever among the infected guinea pigs are in corrlation with the amount of C. burneti in the body of the infected O. moubata.

Q热立克次体以10~(-1)—10~(-12)不同稀释度,用血腔注射法感染非洲钝缘蜱,感染35天,取蜱血淋巴液,分别用Gimenez染色法及免疫荧光技术检查,在10~(-1)—10~(-5)的稀释度,两种方法检出率基本相似。10~(-6)以下各组,免疫荧光尚可检出少数阳性标本,而Gimcnez法则无阳性,前者总阳性率为48.28%,后者为40.68%。感染的蜱悬液接种豚鼠,12组动物血清做Q热补体结合试验全部阳性。动物发病情况如潜伏期长短、发烧天数等似与蜱内立克次体的含量有关。

 
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