助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   被动溶血 的翻译结果: 查询用时:0.38秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

被动溶血
相关语句
  passive hemolysis
     On the other hand,all of the four clones were able to inhibit the binding of Clone 20 to solid phase-bound lipid A (in ELISA) and Re -LPS(in ELISA and passive hemolysis assay).
     在被动溶血试验中,Clone20与固相Re-LPS的结合,也为4个克隆所抑制。
短句来源
  相似匹配句对
     A PASSIVE WOMAN
     被动的女人
短句来源
     hemolytic test;
     溶血试验;
短句来源
     Analysis of 23 patients with child hemolytic uremia syndrome
     小儿溶血尿毒症
短句来源
     On the Structures of the Passive Voice in Ancient Chinese
     上古汉语的被动形式
短句来源
     On the other hand,all of the four clones were able to inhibit the binding of Clone 20 to solid phase-bound lipid A (in ELISA) and Re -LPS(in ELISA and passive hemolysis assay).
     在被动溶血试验中,Clone20与固相Re-LPS的结合,也为4个克隆所抑制。
短句来源
查询“被动溶血”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  passive hemolysis
Definite serological cross-reactivity in the passive hemolysis and the passive hemolysis inhibition tests were demonstrated between LPS fromV.
      
typhi by the method of local passive hemolysis in agar
      
The reaction of passive agglutination with O-antigenS.typhi can be transformed by adding complement into a highly sensitive passive hemolysis test.
      
Use of a stable tetrazonium salt to sensitize erythrocytes with proteins for the local passive hemolysis test
      
After fixation of protein to them by means of a covalent azo-bond, the erythrocytes can be used in the local passive hemolysis test for the detection of rabbit or mouse spleen cells secreting antibodies against protein antigens.
      
更多          


Aim: To obtain broadly crossreactive monoclonal antibodies(McAbs) binding to heterologous lipopolysaccharides(LPS) to eliminate the effects of heterology, extraction and dilution of LPS, and nonspecific interactions Methods: Lipopolysaccharidehigh density lipoprotein(LPSHDL) was prepared according to Cheung′s method for ELISA to screen McAbs, and specificity of crossreactive McAbs were identified by competitive inhibition ELISA and passive hemolysis assay (PHA) Results: Four broadly crossreactive McAbs...

Aim: To obtain broadly crossreactive monoclonal antibodies(McAbs) binding to heterologous lipopolysaccharides(LPS) to eliminate the effects of heterology, extraction and dilution of LPS, and nonspecific interactions Methods: Lipopolysaccharidehigh density lipoprotein(LPSHDL) was prepared according to Cheung′s method for ELISA to screen McAbs, and specificity of crossreactive McAbs were identified by competitive inhibition ELISA and passive hemolysis assay (PHA) Results: Four broadly crossreactive McAbs were obtained out of 13 McAbs Three McAbs and another 1 McAb were screened by ELISA with LPSHDL and LPS as coating antigen respectively Conclusion: The 4 McAbs obtained have broadly crossreactivity, and LPSHDL might be helpful in the selecting broadly crossreactive McAbs binding to heterologous LPS

目的:制备具有广泛交叉反应性的抗LPSMcAb。方法:以内毒素-高密度脂蛋白(LPS-HDL)复合物作为包被抗原,筛选抗LPS的McAb;采用间接ELISA法鉴定其交叉反应性;以竞争抑制试验和被动溶血试验分析交叉反应的特异性。结果:用LPS-HDL筛选获得3株具有广泛交叉反应性的McAb,阳性率为75%(3/4);以LPS筛选出1株具有广泛交叉反应性的McAb,阳性率为11.1%(1/9)。结论:用LPS-HDL包被做ELISA,有利于筛选出具有广泛交叉反应性的抗LPSMcAb。

Clone 20 is a murlne IgM monoclonal antibody raised against Salmonella minnesota R595 and was reported specific for terminal α-3-deoxy-D-manno-octulosonic acid(αKDO) of the inner core of lipopolysacchride.We show in this report that Clone 20 is also able to bind with lipid A,the toxic part of LPS.Anti-Clone 20 hybriaomas were prepared and four antiidiotye antibody (AID)-secreting clones,Clones 47-50,were selected for further characterization.In ELISA inhibition studies,binding of Clones 49 and 50 to solid phase...

Clone 20 is a murlne IgM monoclonal antibody raised against Salmonella minnesota R595 and was reported specific for terminal α-3-deoxy-D-manno-octulosonic acid(αKDO) of the inner core of lipopolysacchride.We show in this report that Clone 20 is also able to bind with lipid A,the toxic part of LPS.Anti-Clone 20 hybriaomas were prepared and four antiidiotye antibody (AID)-secreting clones,Clones 47-50,were selected for further characterization.In ELISA inhibition studies,binding of Clones 49 and 50 to solid phase wbound Clone 20 were inhibited by E.coli J5 (Rc-) and S.minnesota R595 (Re-) LPS,lipid A,and αKDO,whereas Clones 47 and 48 were not inhibited.On the other hand,all of the four clones were able to inhibit the binding of Clone 20 to solid phase-bound lipid A (in ELISA) and Re -LPS(in ELISA and passive hemolysis assay).For binding of Clone 20 to αKDO,Clones 49 and 50 were inhibitory.These results indicate that Clones 47- 50 may bind to,or bind close to,the lipid A-binding site of Clone 20,and that Clones49 and 50 may also bind to .or bind close to,its KDO-binding site.High levels of anti -LPS and anti-lipid A antibodies were detected in Clone 50 ascites.Clone 50 was thus expected as an AID which at least partially mimics .the antigenic structure of Re -LPS.Preliminary animal study showed that intravenous injection of Clone 50 into the mice 3hr prior to K.pneumoniae challenge in mucin -hemoglobin compromised model protected the animal from lethal endotoximia.The protective outcomes raise the interest of development of anti -Clone 20 AIDs as LPS receptor antagonists and/or surrogates of LPS im-munogens in anti-endotoxin immune prophylaxis and therapy.

Clone20系由S.minnesotaR595死菌免疫Balb/c小鼠制备的IgM类单克隆抗体,曾报告它对脂多糖(LPS)中的末端αKDO(2-酮基-3-脱氧辛酸)特异。本文报告采用ELISA显示,Clone20尚能和LPS的毒性成分类脂A结合。用Clone20免疫的小鼠淋巴细胞制备杂交瘤并筛选出4个分泌抗独特型抗体的细胞株.编码为Clone47-50。ELISA抑制试验显示,单克隆抗体Clone49和Clone50与固相Clone20的结合能为E.coliJ5(Re-型)LPS、S.minnesotaR595(Re-型)LPS、类脂A及αKDO所抑制;Clone47和Clone48则否。另一方面,4个克隆均能抑制Clone20结合到团相Re-LPS及类脂A。在被动溶血试验中,Clone20与固相Re-LPS的结合,也为4个克隆所抑制。对于Clone20与αKDO的结合,仅Clone49与50显示抑制作用。这些结果提示,Clone20分子上与Clones47-50的结合部位均不同程度地接近乃至分享其与类脂A结合的独特型决定簇,Clones49与50则尚可结合Clone20上和。KDO结合的独特型...

Clone20系由S.minnesotaR595死菌免疫Balb/c小鼠制备的IgM类单克隆抗体,曾报告它对脂多糖(LPS)中的末端αKDO(2-酮基-3-脱氧辛酸)特异。本文报告采用ELISA显示,Clone20尚能和LPS的毒性成分类脂A结合。用Clone20免疫的小鼠淋巴细胞制备杂交瘤并筛选出4个分泌抗独特型抗体的细胞株.编码为Clone47-50。ELISA抑制试验显示,单克隆抗体Clone49和Clone50与固相Clone20的结合能为E.coliJ5(Re-型)LPS、S.minnesotaR595(Re-型)LPS、类脂A及αKDO所抑制;Clone47和Clone48则否。另一方面,4个克隆均能抑制Clone20结合到团相Re-LPS及类脂A。在被动溶血试验中,Clone20与固相Re-LPS的结合,也为4个克隆所抑制。对于Clone20与αKDO的结合,仅Clone49与50显示抑制作用。这些结果提示,Clone20分子上与Clones47-50的结合部位均不同程度地接近乃至分享其与类脂A结合的独特型决定簇,Clones49与50则尚可结合Clone20上和。KDO结合的独特型决定簇或其邻近结构。此外,在Clone50的腹水中检出高滴度的抗Re-LPS和抗类脂A抗体。综合以上关于Clone50的免疫学特性,推断它至少是部分模拟了LPS的抗原结构。初步的动物实验显示,提前3hr给小鼠注射Clone50,对随后的致死性内毒素休克有明显对抗作用。这一结果提

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关被动溶血的内容
在知识搜索中查有关被动溶血的内容
在数字搜索中查有关被动溶血的内容
在概念知识元中查有关被动溶血的内容
在学术趋势中查有关被动溶血的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社