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框架区
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  framework region
     In framework region sequences displayed Pro at position 9 and Lys at position 67 and identified with the structure patterns of framework residues Ⅱ(A).
     其框架区的9和67位点为脯氨酸(Pro)和赖氨酸(Lys)(非芳香族氨氨酸),符合Ⅱ(A)亚组框架残基结构模式; 其CDR3区含有7个氨氨酸残基,表明C1-INH抗原表面结构并不复杂;
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     In the research, the 3 dimension structure of a vascular endothelial growth factor (VEGF) specific single chain antibody, E11, was predicted by Homology method and then, a humanization strategy named the most similar individual framework region replacement was made by computer aided design on the basis of the structure data.
     本研究以同源模建预测了一株人血管内皮生长因子 (VEGF)特异性鼠源单链抗体E11的三维结构 ,以结构数据为基础并采取单个最相似框架区替代法对其进行人源化设计 ;
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     Objective To identify immune epitopes existed in the framework region (FR) of the IgHV protein of B-cell malignance, and explore the use of these FR-derived peptides to induce the family specific immune response in vitro, in order to explore the possibility of a new IgHV gene family-specific immunotherapy for B-cell malignance.
     目的明确在B淋巴细胞肿瘤表面的免疫球蛋白重链可变区(IgHV)的框架区(FR)上是否存在可以激发细胞毒T淋巴细胞(CTL)的抗原表位,这些来源于FR的抗原肽是否能够激发家族特异性的免疫反应,探讨按照B淋巴细胞肿瘤的IgHV基因分型进行家族性的免疫治疗的可能性。
短句来源
     Methods By single cell isolation and single cell PCR,rearranged immunoglobulin(Ig) genes were amplified from single Ki67(+) cells and single CD3(+) cells. The Ⅴ region family specific primers were designed to Ig heavy chain leader region and light chain κ and λ framework region Ⅰ.
     方法 选取 2例淋巴结反应性增生 ,对Ki6 7和CD3阳性细胞进行单细胞分离和单个细胞聚合酶链反应 (PCR) ,免疫球蛋白家族特异性Ⅴ基因引物分别针对重链引导区、轻链κ和λ框架区Ⅰ。
短句来源
     Results showed that the DNA sequence of the heavy chain gene was highly homologous (>95%) to those of heavy chain genes of known human immunoglobulin family Ⅲ,and the framework region of this heavy chain gene had similarity (>97%) with those of immunoglobulin.
     结果:筛选出抗ABsAg人源噬菌体抗体并发现其重链基因序列与人免疫球蛋白重链基因序列同源性可达95%以上,且与人免疫球蛋白第Ⅲ家族的框架区基因序列同源性均可高达97%。
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  “框架区”译为未确定词的双语例句
     Methods The peptide, QLVQSGAEV, containing IgHV1 frame region 3 rd~11 th amino acids (IgHV1 3~11), was synthesized. IgHV1 3~11-T2 binding tests were performed.
     方法合成Ig重链可变区第1家族框架区3~11位置上的九肽QLVQSGAEV(IgHV13~11),进行T2细胞结合实验。
短句来源
     Study on Labeling of Antisense Oligonucleotides of IgH V1 FR mRNA With ~(125)I
     ~(125)I标记免疫球蛋白重链V1家族框架区反义寡核苷酸
短句来源
     The GDNFβ form is similar to GDNFα except for a 78 bp deletion in the ORF.
     GDNF-β除了在开放阅读框架区(ORF)有78bp的缺失外,其余与GDNF-α相同。
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     Conclusion Cytolytic T lymphocytes generated in vitro against immunoglobulin heavy chain frame-derived nonapeptides can kill B-ALL cells expressing these peptides.
     结论体外诱导的Ig重链框架区九肽特异性CD8+T细胞可杀伤表达该肽的B-ALL细胞。
短句来源
     Objective To induce anti-B-cell acute lymphoblastic leukemia (B-ALL) cytotoxic T lymphocyte response against immunoglobulin heavy chain frame-derived nonapeptide.
     目的用免疫球蛋白(Ig)重链框架区九肽体外诱导抗急性B淋巴细胞白血病(BALL)的细胞毒T细胞应答。
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  相似匹配句对
     NODE AREA DESIGN OF CAST-IN-PLACE REINFORCED CONCRETE FRAME
     钢筋混凝土现浇框架节点设计
短句来源
     Some Problems in Applying Inner-frame Building in Earthquake Area
     在地震采用内框架房屋的问题
短句来源
     The Necessary Conditions for Frames
     框架的必要条件
短句来源
     necking down region;
     颈缩 ;
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     The line density and area density have been counted.
     的密度。
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  framework region
Antibody 7.2 identified a determinant on the framework region of the human Ia antigen.
      
Antibody 9.3 identified a determinant on the framework region of a T-cell antigen.
      
Most variability within the VH1 family is confined to codons 30-32, at the end of the first framework region and in the beginning of the first complementarity-determining region.
      
The cDNA sequences encoding the fourth framework region are consistent with the contribution of four distinct JH segments.
      
We also investigated the cell origin using direct sequence analysis of the complementary-determining region 2 (CDR2) and framework region 3 (FR3) in six cases, consisting of two male and four female patients.
      
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Objective: To acquire the light chain fragment of variable region genes of an antihTNF-a monoclonal antibody. Method: Reverse transcriptional-PCR was applied to amplify the light chain variable region gene from total RNA of a cultured hybridoma cell line E6 which secretes the specific anti-hTNF- α McAb. The gene fragment was amplified with a pair of primers designed according to mouse ig VL gene segment. The PCR product was sequenced using the dideoxy chain termination technique. Results: The VL gene consists...

Objective: To acquire the light chain fragment of variable region genes of an antihTNF-a monoclonal antibody. Method: Reverse transcriptional-PCR was applied to amplify the light chain variable region gene from total RNA of a cultured hybridoma cell line E6 which secretes the specific anti-hTNF- α McAb. The gene fragment was amplified with a pair of primers designed according to mouse ig VL gene segment. The PCR product was sequenced using the dideoxy chain termination technique. Results: The VL gene consists of 342 base pairs and encodes 114 amino acids. It was an open reading frame. There are four FRs, three CDRs and two characteristic cysteine residues within this VL gene. And we have not found the same VL gene in EMBL Gene Bank 1995. Conclusion: The VL gene was confirmed as a functionally rearranged mouse immunoglobulin linght chain variable region gene.

目的:获得鼠抗人肿瘤坏死因子-α(hTNF-α)单克隆抗体轻链可变区基因序列。方法:采用反转录PCR(RT-PCR)方法,以一对针对鼠Ig轻链可变区(VL)基因的引物,从分泌鼠抗hTNF-α单克隆抗体的E6杂交瘤细胞株中扩增和克隆VL基因片段,用双脱氧链终止法测定其核苷酸序列,并进行计算机分析。结果:此VL基因长342bP,可编码114个氨基酸,为开放读框;有明确的框架区(FRS)和抗原互补决定区(CDRS);含有抗体可变区特征性的两个半胱酸残基。在基因数据库(EMBLGeneBank1995)中,未查见相同序列的基因。结论:此VL基因系重排的鼠Ig轻链基因。

In this paper,RNA extracted from hybridoma cells F 7 secreting specific McAb against human C1 INH was transcribed reversely into cDNA with oligo(dT) 15 .The first strand cDNA was used as templet for PCR to amplify immunoglobulin variable region genes using universal primers. The variable region of heavy chain(VH) gene was isolated and subcloned into pUC18/19 vectors, and sequenced by Sangers dideoxy mediated chain termination method. It...

In this paper,RNA extracted from hybridoma cells F 7 secreting specific McAb against human C1 INH was transcribed reversely into cDNA with oligo(dT) 15 .The first strand cDNA was used as templet for PCR to amplify immunoglobulin variable region genes using universal primers. The variable region of heavy chain(VH) gene was isolated and subcloned into pUC18/19 vectors, and sequenced by Sangers dideoxy mediated chain termination method. It shown that VH gene consisted of 333bp encoding 111 amine acid resides. Comparing with EMBL and Kabat database, the VH was in agreement with the characterization of DNA sequence present in the mouse Ig VH region. According to Kabat classified method, the deduced amino acid sequence of F 7 VH gene belonged to the mouse Ig VH subgroup Ⅱ(A) and was resulted from V H D J H3 rearrangement. In framework region sequences displayed Pro at position 9 and Lys at position 67 and identified with the structure patterns of framework residues Ⅱ(A). The success of cloning of the VH gene of F 7 McAb lay a good foundation for the construction and expression of single chain variable fragment of F 7 McAb.

由分泌抗人C1-INHMcAb的杂交瘤F7细胞株提取总RNA,合成与VH基因FR1和FR4互补的通用引物,以RNA反转录合成的第一链cDNA为模板,PCR法克隆出F7VH基因的DNA片段。将分离获得的目的DNA片段亚克隆入pUC18/19测序载体,从两头进行双脱氧核苷酸随机终止法的DNA序列测定。结果显示:VH基因是由333个碱基组成,编码111个氨基酸残基。通过国际联机检索进行EMBL和Kabat基因库扫描发现,F7VHDNA仅与Ig同源,符合小鼠Ig的VH基因特征;同源性为60%~85%,应归属于Ig的VH基因。根据Kabat分类方法,F7VH基因推导的氨基酸顺序属于小鼠Ig的VH基因的Ⅱ(A)亚组,是由VH-D-JH3重排产生;其框架区的9和67位点为脯氨酸(Pro)和赖氨酸(Lys)(非芳香族氨氨酸),符合Ⅱ(A)亚组框架残基结构模式;其CDR3区含有7个氨氨酸残基,表明C1-INH抗原表面结构并不复杂;FR2和FR3的22和89位点为半胱氨酸残基(Cys),与VH片段二硫键形成有关。成功获得F7VH基因为进一步构建和表达单链Fv(scFv)抗体打下良好的基础。

The phage display system is the latest technique by which human monoclonal antibodies can be made in vitro. We have obtained several phage antibodies (PhAb) binding to E.coli J5 strain from human combinatorial immunoglobulin libraries by using this technique. The genes encoding heavy chains from two PhAbs to E.coli J5 strain were subcloned into M13mp 18 phage vector and the immunoglobulin heavy chain variable(V H) gene of selected recombinant Clone J8 and B11 were then sequenced. The results revealed that...

The phage display system is the latest technique by which human monoclonal antibodies can be made in vitro. We have obtained several phage antibodies (PhAb) binding to E.coli J5 strain from human combinatorial immunoglobulin libraries by using this technique. The genes encoding heavy chains from two PhAbs to E.coli J5 strain were subcloned into M13mp 18 phage vector and the immunoglobulin heavy chain variable(V H) gene of selected recombinant Clone J8 and B11 were then sequenced. The results revealed that the DNA sequences of these two Clone V H genes were highly homologous (>97%) with those of V H genes of known human immunoglobulin family Ⅲ, and over and with those of genes of the heavy chain variable regions of 99% of sequence similarities within framework region of immunoglobulin were also demonstrated. This indicates that the two PhAbs to E.coli J5 strain contain the V H genes of human antibodies variable regions of human antibodies.

噬菌体抗体库技术是近年来发展起来的在体外制备人源抗体的新技术。我们曾用该项技术筛选出能与大肠杆菌J5株结合的人源噬菌体抗体(PhAb)。本文将两株能与大肠杆菌J5株结合的噬菌体抗体重链基因,经亚克隆M13mp18噬菌体载体后,测定其重组噬菌体中插入基因的DNA序列。结果证实,J8和B11两克隆重链可变区(VH)基因序列与已报道的人免疫球蛋白VH基因序列的同源性达97%以上;且与人免疫球蛋白第Ⅲ家族的框架区基因序列同源性高达99%,表明此两株能与大肠杆菌J5株结合的阳性克隆均含有人抗体VH基因片段。

 
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