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苏丹黑
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  sudan black
     Secondly,Sudan black B staining:(1)Rinse section in 70% ethanol for 1—2 min, (2)Stain in fresh 1% Sudan black B in 70% ethanol for 30—60 min at 40—60℃,(3)Rinse in 70% ethanol for 1 min and then in distilled water.
     第二步,苏丹黑 B 液染色:(1)70%酒精中1—2分钟; (2)新鲜配制的0.3%苏丹黑 B 液(70%酒精配制)中染色,40—60℃,30分钟至1小时;
短句来源
     Sudan black staining found more granules in the 72mg/kg K-SeC groups.
     苏丹黑染色胞浆颗粒以72mg/kg组稍多。
短句来源
     Objective:Two cases of acute promylocytic leukemia with microgranular variant(M 3v ) with negative stains of MPO(myeloid peroxidase, MPO)and SBB(sudan black B, SBB) were studied.
     目的 :探讨急性变异型早幼粒细胞性白血病 (M3v)髓系过氧化物酶 (MPO)和苏丹黑B(SBB)染色缺乏的意义。
短句来源
     Cytochemistry evaluation showed that MSCs were homogeneously positive for acid α-naphthl acetate esterase (ANAE), glycogen (periodic acid Schiff reaction, PAS), and negative for acid phosphatase (ACP), Sudan black reaction (SB) and that approximately 5% of them was positive for alkaline phosphatase (ALP).
     细胞化学结果显示 ,几乎所有细胞酸性萘酚醋酸酯酶 (ANAE)及糖原 (PAS反应 )阳性 ,酸性磷酸酶 (ACP)及苏丹黑反应 (SB)阴性 ,约5 %细胞碱性磷酸酶 (ALP)阳性。
短句来源
     The cyst fluid showed 10 bands in PAGE and 16 bands in SDS-PAGE stained by Coomassie brilliant blue R-2SO, one band stained by PAS and another by Sudan black B. 14 IE antigenic components in cyst fluid were demonstrated.
     囊液抗原有10条(PAGE)和16条(SDS-PAGE)考马斯亮蓝染色带、PAS和苏丹黑B染色带各1条,免疫学抗原14种。
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  “苏丹黑”译为未确定词的双语例句
     The results showed that high homogenous MSCs had unique immunophenotypes and they were positive for CD73,CD105,CD44,CD166 and CD90 but negative for CD34,CD_(31) and CD45.Cytochemistry showed that MSCs were positive for glycogen,and negative for SB and AKP.
     结果表明,分离、培养出高度同源性的MSCs具有独特的细胞免疫学表型,即CD73、CD105、CD44、CD166、CD90阳性,CD34、CD 31、CD45阴性; 细胞组织化学MSCs PAS-过碘酸雪夫氏染色阳性,苏丹黑-B(SB)及碱性磷酸酶(AKP)染色阴性;
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     Results MPO and SBB staining are negative in patients with CLL.
     结果CLL的骨髓过氧化物酶(MPO)和苏丹黑染色(SBB)为阴性;
短句来源
     PAS was positive,and SB was negative.
     细胞糖原反应 (PAS)阳性、苏丹黑反应 (SB)阴性。
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     The results of hot test, periodate oxidation, SDS-PAGE, immuno-blotting and immunological studies suggestd that MC5 corresponding antigen is a new colon cancer associated glycoproteirr with molecular weight of 67 and 55Kd.
     用免疫亲和层析的方法从结肠癌组织中纯化出单抗MC_5相应抗康,经电泳乙酰苏丹黑10B染色,考马斯亮蓝染色,加热实验,高碘酸氧化,SDS—PAGE和免疫印迹等分析证实MC_5相应抗原是一种新的结肠癌相关糖蛋白,分子量分别为67KD和55KD。
短句来源
     Methods: To separate the serum lipoproteins prestained with Sudan BlackB in the first double gradient polyacrylaniide gel electrophoresis (f-DGPAGE).
     方法:将血清Lp用苏丹黑B(SB-B)预染,采用一阶双梯度聚丙烯酰胺凝胶电泳法(f-DGPAGE),使血清Lp在电势差、温度差、浓度梯度差和分子筛的共同作用下,分离出11种组分和亚组分。
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  相似匹配句对
     Black start for power grid in Sudan
     苏丹电网的启动
短句来源
     Experiment of black start in Eigili power station in Sudan
     苏丹EIGILI电厂“启动”试验
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     Black Gold
     [金]
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     The Dark Eye
     眼睛
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     A Glimpse of the Customs of Sudan
     苏丹风俗一瞥
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  sudan black
The optical torque of the nematic liquid crystals was strongly enhanced by a small amount of dye impurities (Sudan Black B).
      
Screening for PHA production was done by Sudan black staining and nine out of sixteen strains exhibited PHA producing ability.
      
Upon density gradient ultracentrifugation of serum prestained for lipid with Sudan Black, it was observed that dietary cholesterol caused the HDL1 particles (1.063>amp;lt;d>amp;lt;1.092) to shift towards a lower density range.
      
Staining of resin sections with Sudan Black was positive.
      
With PAS staining, a slight decrease in reactivity after 8±2 weeks could be observed, whereas all other stainings tested (POX, Sudan black B and Naphthol-AS-D-esterase) were unaffected even by a delay of up to 16 weeks.
      
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From 32 rhesus monkeys the vermis cerebelli and the portion containing the dentate nucleus were fixed in 10% formalin.The animals of both sexes were divided into 4 age groups according to their dental status.Paraffin sections as well as frozen sections were prepared.The Purkinje cells per unit area of the cortex were estimated on sec- tions stained with methyl green and pyronin.With advancing of age the numbers of the Purkinje cells decreased gradually,with an average reduction from 1391 to 1065 in the males...

From 32 rhesus monkeys the vermis cerebelli and the portion containing the dentate nucleus were fixed in 10% formalin.The animals of both sexes were divided into 4 age groups according to their dental status.Paraffin sections as well as frozen sections were prepared.The Purkinje cells per unit area of the cortex were estimated on sec- tions stained with methyl green and pyronin.With advancing of age the numbers of the Purkinje cells decreased gradually,with an average reduction from 1391 to 1065 in the males and from 1203 to 1108 in the females.Histochemically the lipofuscin pigments differed from melanin and hemosiderin.They were almost insoluble in fat solvents,readily stained by Sudan black B,and were PAS-positive.The pigments in group Ⅳ usually appeared acidfast with the long Ziehl-Neelsen method,while those in the younger age groups were stained poorly or not at all.With Schmorl's ferricyanide method,only the pigments in the Purkinje cells of group Ⅳ reacted positively.Theese characteristics indicated that the pigments belonged to the ceroid-type lipofuscin as des- cribed by Pearse (1960).The magnitude of the pigments in both types of cells was es- timated in the Sudan black B-stained sections.In both sexes the numbers of pigmented cells and the cells with higher grades of pigmentation were found to be increased con- comitantly with the increase of age.The difference were statistically significant.The pigments of Purkinje cells were more or less uniform in size and accumulated lateral to or above the nuclei.The pigments in the neurons of dentate nucleus varied in size and distribution in the cytoplasm.In both types of the cells pigments were found occasion- ally in the dendrites.

用狝猴32只,分雌雄两批,按齿序情况分为4个年龄组。取小脑蚓体及含齿状核的部分,用10%福尔马林固定,作石蜡切片及少量冰冻切片。在甲绿(口派)啷咛染色的切片止, 计算了蚓体局部皮质内单位面积上的浦肯野细胞的数量,随年龄增加浦肯野细胞数递减。在雄性平均值由1391减至1065,在雌性由1203减至1108。用组织化学方法证明齿状核神经元及浦肯野细胞内的色素不是黑素或含铁血黄素,几乎不被脂溶剂溶解,易染苏丹黑,PAS反应阳性。用Ziehl-Neelsen长法染色第Ⅳ年龄组普遍有抗酸性,低年龄组反应较弱,成为阴性。对Schmorl氏铁氰化物,仅第Ⅳ组浦肯野细胞内色素呈阳性反应。这些性质表明色素为Pearse所描述的类蜡型脂褐素。在苏丹黑染色切片上,将两类细胞的脂褐素含量分别确定等极,计算了各年龄组内含各级色素的细胞所占百分数。在雌雄两性,含色素的细胞数,及色素合量高的细胞数,均随年龄增进而加多。这些数据经统计学分析,证明差异显著。在浦肯野细胞内色素大致均匀,分布多集中于胞核两侧或上端;在齿状核细胞内色素大小与分布很不一致,两类细胞中均可偶见分布到树突中的色素。

Sixty four albino mice (aged 50 days) and 41 albino rats (aged 124 days) were

用50天小白鼠64只及124天大白鼠41只,各分4组:Ⅰ组均切除左肾;Ⅱ组均在皮下注射ACTH隔日一次,剂量为每百克体重0.33国际单位,但其中大白鼠在注射前10天先切去左肾,小白鼠不切;Ⅲ组均先切除左肾,10天后注射ACTH,其中小白鼠所用剂量与Ⅱ组同,大白鼠则用15倍于Ⅱ组的剂量;Ⅳ组大、小白鼠均作对照。小白鼠自注射25次开始分批取材,最后一批注射达78次。大白鼠在注射24次后即全部杀死。两批材料均取颈膨大处的脊神经节及同节脊髓,用福尔马林固定,制成石蜡切片。在苏丹黑及PAS染色的切片上观察了神经细胞内呈阳性反应的脂褐色素,计算了在不同处理下含色素细胞所占百分率。并根据色素在细胞内含量的不同等级,比较了不同处理下含各级色素的细胞数量。对所得数据进行了统计分析。结果表明各项实验处理都没有使含色素的神经细胞比对照有所增多,也没有提高细胞内的色素含量。以大白鼠为例,上述四组中含色素的神经细胞所占百分率由Ⅰ到Ⅳ分别为3.31、4.00、3.40及3.70%。各实验组与对照的差异均无统计学意义。以上结果与Sulkin在1960年发表的研究结果不同。他认为去肾及去肾后注射ACTH的大白鼠神经细胞比对照有更...

用50天小白鼠64只及124天大白鼠41只,各分4组:Ⅰ组均切除左肾;Ⅱ组均在皮下注射ACTH隔日一次,剂量为每百克体重0.33国际单位,但其中大白鼠在注射前10天先切去左肾,小白鼠不切;Ⅲ组均先切除左肾,10天后注射ACTH,其中小白鼠所用剂量与Ⅱ组同,大白鼠则用15倍于Ⅱ组的剂量;Ⅳ组大、小白鼠均作对照。小白鼠自注射25次开始分批取材,最后一批注射达78次。大白鼠在注射24次后即全部杀死。两批材料均取颈膨大处的脊神经节及同节脊髓,用福尔马林固定,制成石蜡切片。在苏丹黑及PAS染色的切片上观察了神经细胞内呈阳性反应的脂褐色素,计算了在不同处理下含色素细胞所占百分率。并根据色素在细胞内含量的不同等级,比较了不同处理下含各级色素的细胞数量。对所得数据进行了统计分析。结果表明各项实验处理都没有使含色素的神经细胞比对照有所增多,也没有提高细胞内的色素含量。以大白鼠为例,上述四组中含色素的神经细胞所占百分率由Ⅰ到Ⅳ分别为3.31、4.00、3.40及3.70%。各实验组与对照的差异均无统计学意义。以上结果与Sulkin在1960年发表的研究结果不同。他认为去肾及去肾后注射ACTH的大白鼠神经细胞比对照有更多的色素沉积现象。本文在讨论中对比了双方的工作,就Sulkin室实验中的动物只数、研究方法以及他所提出的“应激”反应进行了评述。最后指出,神经细胞内脂褐素的实验性形成问题还远未解决。因此还没有理由完全否认色素沉积是一种内生的衰老变化的指标。

A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of tris...

A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of tris with EDTA and boric acid concentrations at 0.017 M and 0.061 M respectively; potential gradient 5~6 volts/cm and a current of 0.75~1 mA/strip.

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2...

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2±29.3毫克/100毫升。四、讨论了不同类型高脂蛋白血症中脂蛋白的异常及分型在临床应用上的意义。

 
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