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纸电泳
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  paper electrophoresis
     The result of high voltage paper electrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root and regeneration plant.
     经高压纸电泳检测 ,菘蓝毛状根及其再生植株中均含有甘露碱 ,表明 Ri质粒的 T- DNA已整合进毛状根和再生植株中。
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     The base ratio as determinated by paper electrophoresis and chromatography was A: G: C: U=30.3: 16.3: 16.7: 36.6. SDS polyacrymide gel experiments revealed three major peptides of 140, 000(L), 46, 000(N), 40, 000 (NS) daltons.
     碱水解及S_1核糖核酸酶酶解实验证明小麦丛矮病毒的基因组为单链RNA,双向纸电泳及层析方法测定其碱基组成比例为A=30.3,G=16.3,C=16.7,U=36.6。
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     DETERMINATION OF Cr(Ⅲ) AND Cr(Ⅵ) IN ELECTROPLATING EFFLUENT SIMUTANEOUSLY BY PAPER ELECTROPHORESIS AND PHOTO-DENSITY SCANNING PROCESS
     应用纸电泳-光密度扫描法同时测定电镀废水中的Cr(Ⅲ)和Cr(Ⅵ)
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     Methods The contents of glutamate and acidic peptide in the brains of rats were measured by paper electrophoresis,ninhydrin colouring,spectrophotometry and standard curve method.
     方法通过纸电泳、茚三酮显色、分光光度法和标准曲线法测定脑内谷氨酸和酸性肽的含量。
短句来源
     Three polysaccharides,namely AP-1,AP-2,AP-3,which were proved to be homo-geneous by glass fiber paper electrophoresis,ultracentrifugation analysis and gel chromatography,were isolated from the stems of Dendrobium aphyllum The limiting viscosity numbers of three polysaccharides were 426.96, 416.96 and 234.03 respectively.
     从兜唇石斛(Dendrobiumaphyllum)的茎中提取到3种多糖:AP-1,Ap一2,Ap一3,玻璃纤维纸电泳、超速离心和凝胶层析证明:三者都为单一均匀成分。 3种多糖的特性粘数([n])分别为426.96,416.96,234.03;
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  “纸电泳”译为未确定词的双语例句
     Two of watersoluble polysaccharides,AsⅢa and AsⅢb,were gained by colum chromatography on DEAE cellulose and Sephadex G150.Both AsⅢa and AsⅢb were judged to be homogeneous by the techniques of colum chromatography and electrophoresis.
     AsⅢa和AsⅢb经醋酸纤维薄膜电泳、玻璃纤维纸电泳和凝胶柱层析分析,证明都是均一的,经琼脂糖凝胶柱层析测得AsⅢa和AsⅢb的分子量分别为85KD和49KD左右。
短句来源
     Hydrolytic ATP used 0.3mol barium hydroxideat 96℃ for 40min, yielde cAMP The cAMP of sample was seperated by paper electropnoresis,its con tent tested by ultravioletabsorption。
     用0.3mol Ba(OH)_2 96℃加热40min水解ATP制得cAMP,纸电泳分离样品中的cAMP,紫外吸收测定其含量。
短句来源
     ②Transformation of Ti and Ri plasmids was inspected by high-pressure-paper electrophoresis;
     ②用纸电泳检测Ti和Ri质粒是否转化成功 ;
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     Paper electrophoretic pattern and UV absorption spectrum are in accord ance with the characters of tetar-alkene antibiotic. There are three peaks at λmax 292,λmax 305和λmax 320 nm respectively;
     其纸电泳结果和紫外吸收特性符合四烯类抗生素的图谱特征,后者在波长λmax292,λm ax305和λm ax320 nm处表现为典型的3个吸收峰.
短句来源
     Purity detection indicated that DTA was homogeneous by gel filtration chromatography on Sephadex G-100 and cellose acetate pellicle electrophoresis.
     采用葡聚糖凝胶G-100柱层析和醋酸纤维素薄膜纸电泳检测DTA的纯度,实验显示:DTA为单一多糖,利用高效液相色谱分析DTA的组成。
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  相似匹配句对
     The Experimental Technology and Operation Outline of the Paper Electrophoresis for Amino Acid
     氨基酸电泳实验技术和操作
短句来源
     Recent Development of Electronic Paper Based on Electrophoresis
     基于电泳技术的电子研究进展
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     FLUORIMETRIC ANALYSIS USING FILTER PAPER AS THE SUBSTRATE
     上荧光分析法
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     The Paper Crane
    
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     Electrophoretic Painting
     电泳着色
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  paper electrophoresis
A technique involving the use of paper electrophoresis is described for the study of binary and mixed-complex systems in solution.
      
Stability constants of oxalate complexes of copper (II) and nickel (II) by paper electrophoresis
      
Stability constants of copper (II) and nickel (II) oxalates have been determined by paper electrophoresis.
      
Stoichiometric stability constants of Cu(II), Ni(II), Zn(II), Co(II), UO2(II) and Th(VI) phthalate have been determined by paper electrophoresis.
      
A method using high-voltage paper electrophoresis to separate 2-alkoxy-l-methylpyridinium p-toluenesulphonates derived from alcohols is described.
      
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Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶胰蛋白酶及结晶胰凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

Prompted by the need for analysing amino acids in very small samples of nervous tissue in physiological work, an ultramicro method of electrophoresis on cellophane paper instead of filter paper has been developed. The method described permits the determination of γ-amino butyric, glutamic and aspartic acids in quantities of the order of millimicrograms. Its sensitivity is thus about 200 times that of the ordinary paper electrophoresis or chromatography. Its accuracy as judged by self-consistency in repeated...

Prompted by the need for analysing amino acids in very small samples of nervous tissue in physiological work, an ultramicro method of electrophoresis on cellophane paper instead of filter paper has been developed. The method described permits the determination of γ-amino butyric, glutamic and aspartic acids in quantities of the order of millimicrograms. Its sensitivity is thus about 200 times that of the ordinary paper electrophoresis or chromatography. Its accuracy as judged by self-consistency in repeated trials or by recovery tests of known amounts of amino acids, approaches that of the usual method. It is also capable of separating β-alanine, cysteic acid, cysteine and basic amino acids in alkaline solutions.

(一)为了滿足生理学研究中微量生物材料的分析,我們創立了一个用玻璃紙做材料的超微量电泳方法。能測量毫微克范围內的γ-氨基丁酸、谷氨酸和門冬氨酸。 (二)本方法的灵敏度为5×10~(-9)克,誤差范围±7%,与一般纸层析相較,精确度相差不多,而灵敏度提高了两个数量級。 (三)除上述氨基酸外,玻璃紙电泳还能分开β-丙氨酸,磺酸丙氫酸,半胱氫酸和碱性氨基酸等;用本法我們測定了一些生物样品中的游离氨基酸合量,符合用纸层析所得的結果。

(1)A large amount of polyol was found in the mycelium of G. candidum and identified as mannitol by paper chromatography, paper electrophoresis and melting point determinations of the recrystallized polyol and its tetra-acetate derivative.(2)A NADP-specific mannitol dehydrogenase was found in the cell-free extracts of G. candidum, it catalyzed the oxidation of mannitol to fructose by NADP. The reaction was reversible.(3)A phosphatase(or phosphatases)catalyzing the hydrolysis of fructose phosphate esters to free...

(1)A large amount of polyol was found in the mycelium of G. candidum and identified as mannitol by paper chromatography, paper electrophoresis and melting point determinations of the recrystallized polyol and its tetra-acetate derivative.(2)A NADP-specific mannitol dehydrogenase was found in the cell-free extracts of G. candidum, it catalyzed the oxidation of mannitol to fructose by NADP. The reaction was reversible.(3)A phosphatase(or phosphatases)catalyzing the hydrolysis of fructose phosphate esters to free fructose and inorganic phosphate was also found in the cell-free extracts.(4)No NAD(P)H_2-fructose-6-phosphate reductase activity was detected in the cellfree extracts.(5)The pathway of mannitol formation in G. candidum was established as:D-Fructose-phosphate→Pi→D-Fructose→NADPH_2 NADP→D-Mannitol(6)In xylose-grown mycelium of G. candidum a NAD-specific L-iditol dehydrogenase was adaptively formed, it catalyzed the following reactions:Xylitol→NAD NADH_2→D-Xylulose and D-Sorbitol→NAD NADH_2→D-Fructose.

(1)白地霉菌絲中含有大量的多元醇,經紙层析、紙电泳及提純結晶制备衍生物等方法鉴定为甘露醇。(2)白地霉无細胞提取液中有NADP-甘露醇脫氫酶存在,催化甘露醇被NADP氧化为果糖或相反地果糖被NADPH_2还原为甘露醇。(3)白地霉无細胞提取液中有磷酸酯酶,催化果糖磷酸酯水解为自由果糖及无机磷酸。(4)白地霉中測不出果糖-6-磷酸还原酶活力。(5)确定了甘露醇的形成途径为: D-果糖磷酸→Pi→D-果糖←NADPH_2 NADP→D-甘露醇(6)D-木糖培养的白地霉适应形成了NAD-艾杜糖醇脫氫酶,催化: 木糖醇←NAD NADH_2→D-木酮糖D-山梨醇←NAD NADH_2→D-果糖

 
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