Conclusions It indicates that KMB17,A549and BEL7402cells are sensitive to HEV under the experimental culture conditions,while Hela and Vero cells are insensitive. Tissue culture system of HEV in certain filial generation is established.
In order to establish the tissue culture system of tall fescue(Festuca arundinacea) for the transgenic study,callus induction and plantlet regeneration of sterilized mature seeds of tall fescue varieties were studied.
(6) 1/2MS+Sucorse 30g L-1 All the above media were added 100 mg L-1 inositol and 0.75 % agar. In this study, the best medium MS + Sucrose 20 g L-1 for the growth of seedlings was selected.
Plump ripe seeds of Actinidia deliciosa used as initial material were treated with gibberellin at 2 5g·L -1 for 5 h,then sterilized with sodium hypochlorite. The germinated seeds were inoculated on the MS medium containing 20g·L -1 sucrose,100 mg·L -1 inosite ,and 0 75%agar.
Fragments of human adenoid tissue were grown in a tissue culture system where all artificial tissue handling, except for the explanation procedures and tissue culture condition in itself, was excluded.
A tissue culture system of the rat embryonic central nervous system is described which allows morphological, biochemical, and electrophysiological approaches.
For establishing the medium system of induced prunus persica callus,the combination of MS medium with different hormone had been used.The content of amygdalin had been determined with the method of derivative spectrum scanning.The results showed that in the combined medium of MS+IAA+BA,the inducing rate of callus was the highest(33.33%) while the growth rate in medium MS+NAA+BA was the highest.The content of amygdalin in MS+IAA+BA(0.0273%)was l...
Objective To investigate the hepatitis E virus(HEV)sensitive cells and its tissue culture condi -tions.Methods The HEV from dejecta supernatant of patients with acute hepatitis E was amplified an d activated by passaged in Rhesus.Then,the positive dejecta samples of infected monkeys were dealed with super-centrifugation and virus for culture was obtained.Various human-derived(including KM B17,A549,BEL7402,and Hela)and non-human primates derived cells(Vero)were inoculated with HEV.Sensitivity of cells to H...
The initiation of maize immature embryo is more difficult than other crops because it is influenced by more factors.Besides genotype and physical conditions,the concentration of 2,4_D and embryo age are important factors.In this paper,we analized their influence on the process of initiation,and concluded the proper concentration of 2,4_D and the proper embryo ages of inbred lines of Ji853,Ji842,4112.