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scar标记     
相关语句
  scar marker
     Cloning of RAPD marker and studying of translating to SCAR marker on F gene of peach (Prunus persica(L.) Batsch)
     桃(Prunus persica (L.) Batsch) F基因RAPD标记的克隆及SCAR标记的转化研究
短句来源
     Conversion OPW03-900 of RAPD which linked to pollen sterility gene to SCW03-906 of SCAR marker.
     把与桃花粉育性基因连锁的RAPD标记OPW03-900转化为SCAR标记SCW03-906,该序列已被Genebank收录,登录号为DQ659676。
短句来源
     SCAR marker linked to seedless genes in grapes and southern blot analysis
     葡萄无核基因的SCAR标记及Southern blot分析
短句来源
     The E-ACG/M-CAG-180 marker had transferred into a co-dominante SCAR marker(SCAR3-109), and the genetic distances was 11 cM.
     将E-ACG/M-CAG-180特异片段转化成共显性的SCAR标记SCAR3-109,与ZYMV-CH的抗性基因遗传连锁距离为11cM。
短句来源
     Based on the sequence of the polymorphic DNA fragment, one specific primer pair was designed with the software of Primer Premier 5.0(SC1:5'-ACAAGTTGGAGTCAGGAG -3'; SC2:5'- ATCGTATGGTTTCGTCTT -3')- The AFLP marker was transformed to a sequence characterized amplified region(SCAR) marker, and all isolates were tested with the SCAR marker.
     根据该特异性DNA片段测序结果,用Primer Premier 5.0软件分析其序列,并设计出一对特定引物(SC_1:5′-ACAAGTTGGAGTCAGGAG-3′;SC_2:5′-ATCGTATGGTTTCGTCTT-3′),用此引物对可定性扩增出小麦光腥黑粉菌DNA特异性区段,将AFLP分子标记转化为SCAR标记
短句来源
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  scar markers
     According to the sequences, two pairs of specific primers were designed and synthesized. Three of them (P34M54_(421), P32M59_(208), P36M53_(164)) were successfully converted into SCAR markers, i. e. SC-P34M54_(378), SC-P32M59_(147) and SC-P36M53_(99).
     将其中的P34M54_(421)、P32M59_(208)、P36M53_(164)成功转化成了SCAR标记,即SC-P34M54_(378)、SC-P32M59_(147)和SC-P36M53_(99)。
短句来源
     Translating of F Gene and Y Gene of Peach(Prunus Persica(L.) Batsch) to SCAR Markers
     桃(Prunus persica(L.) Batsch)白肉机因(Y)和离核基因(F)的SCAR标记转化
短句来源
     The SCAR primers were successfully amplified by PCR method betweenY98148 and Y98149. Consequently, RAPD markers S1041_(525)、S1076_(549) and S1272_(403) weresuccessfully converted into SCAR markers SCS1041498、SCS1076510 and SCS1272388.
     根据所测的序列设计特异性引物,并在近等基因系间进行了 PCR 分析,成功地将RAPD 标记 S1041_(525)、 S1076_(549)、S1272_(403)转化为 SCAR 标记 SCS1041_(498)、 SCS1076_(510) 、SCS1272_(388);
短句来源
     RAPD markers OPAI17-1550 and OPAI13-900,linked to anti-black-spot disease gene locus,were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13292 were validated in the resistant、susceptible pools and F1 population.
     通过测序和引物设计,将与美洲黑杨抗黑斑病基因相连锁的RAPD标记OPAI17-1550和OPAI13-900成功地转化成显性SCAR标记(SAI17-1570)和共显性SCAR标记(SAI13-292),并对感、抗病池和F1代91个无性系进行了SCAR标记检测。
短句来源
     5. RAPD_S312 and RAPD_S360, the RAPD markers,were successfully converted into SCAR markers named CBJ312,CBJ360 respectively. CBJ312 and CBJ360 were validated in the parent pools and F2 population.
     5、成功地将RAPD_S312、RAPD_S360分别转化成SCAR标记CBJ312和CBJ360,并对对生玉米亲本池、互生池、F_2单株进行了SCAR标记检测,在对生池和F_2的RAPD_S312阳性单株中CBJ312的阳性率为100%,F_2的RAPD_S312阴性单株没有检测到CBJ312扩增;
短句来源
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  a scar marker
     The RAPD marker was successfully converted into a SCAR marker with primer combination BFP96/BFP98,which was designated SCP20-2258.The linkage distance is 7.8 cM between the SCP20-2258 and the peach/nectarine character.
     利用引物对BFP96/BFP98成功将RAPD标记转化成了SCAR标记,并命名为SCP20-2258。
短句来源
     The OPA18 432 marker was testified to be closely linked to the M4 gene with a genetic distance of 2.1cM through the analysis of the F 2 mapping population derived from a cross of Bison×NM4. Based on the sequence of OPA18 432 , the specific PCR primers were designed,and a SCAR marker for the M4 gene was produced.
     用Bison与NM4杂交产生的F2 分离群体进行的遗传连锁性分析表明 ,RAPD标记OPA1843 2 与M4基因紧密连锁 ,二者之间的遗传距离为 2 .1cM。 将OPA1843 2 片段回收、克隆和测序 ,成功地将其转化为SCAR标记
短句来源
     Development of a SCAR marker linked to avirulence gene AVR-Pik~m in rice blast fungus Magnaporthe grisea
     一个与稻瘟病菌无毒基因AVR-Pik~m连锁的SCAR标记的分离
短句来源
     A SCAR marker was conversed from RAPD marker of Haihe3 maize hybrids. The result of SCAR and RAPD test in Haihe3 hybrids seed purity were identical.
     建立了海禾 3纯度分析的SCAR标记 ,该SCAR标记与RAPD标记在种子纯度检测上结果完全一致。
短句来源
     The cloned fragment AG/CAG was sequenced and then converted successfully to a SCAR marker, which can be used more conveniently in the identification and marker assisted selection for gene FuJ7(t) to flax wilt.
     将AG/CAG片段回收、克隆和测序 ,成功地将其转化为SCAR标记 ,可以更加方便地用于对FuJ7(t)基因的分子检测和标记辅助选择。
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  “scar标记”译为未确定词的双语例句
     RAPD marker S1338_(656) and AFLP marker P67M54_(172) were sequenced and successfully transferred into SCAR markers-SCOR_(204) and SCOR_(127) individually.
     将筛选到的连锁RAPD标记S1338_(656)和AFLP标记P67M54_(172)成功转化为SCAR标记SCOR_(204)和SCOR_(127)。
短句来源
     Positive colony was identified for sequencing. According to the sequences, three pairs of 22-mer specific primers, SCS13L/SCS13R, SCS200L/SCS200R1 and SCS200L/ SCS200R2 were designed.
     根据测序结果,设计了3对SCAR引物:SCS13L/SCS13R(S132800的SCAR标记引物)、SCS200L/SCS200R1、SCS200L/SCS200R2(S2002400的SCAR标记引物)。
短句来源
     Further, transform the RAPD mark of RS and RM to SCAR mark PS-1(FP:5'ttccccccagtacgcaataac3', RP;5'ttccccccagacgtcacgtact3') and PM-1(FP:5'ttcccccagtgatggaatttacg3', RP:5'ttccccccagtccaatgttaca3'). Thus, Suju, Minghu and their F1 were identified accurately and fast, with the synthetic specific primers of PS-1 and PM-1.
     进一步根据RS和RM序列合成SCAR标记引物PS-1(FP:5’ttccccccagtacgcaataac3’,RP:5’ ttccccccagacgtcacgtact3’)和PM-1(FP:5’ttcccccagtgatggaatttacg3’,RP:5’ttccccccagtccaatgttaca3’),能够准确、快速地鉴别所标记的苏菊、明虎及其F1蚕品种。
短句来源
     In the analyses of compositive effects, S_1~- S_2~+, J~-S_1~- ,J~-S_2~+ and J~-S_1~-S_2~+ have significant effects on 12,18 and 43-week weights.
     在SCAR标记分析中,S_1标记和S_2标记分别对京海Ⅰ号黄鸡12周龄、18周龄和43周龄体重有显著影响,其中,S_1标记表现为减效效应,而S_2标记表现为增效效应。
短句来源
     We identified 99-2439 as a Triticum aestivum -Secale cereale 1BL/1RS alien translocation line by chromosome C-banding and 1RS-specificSCAR marker detection.
     通过染色体C-分带和1RS染色体特异性SCAR标记鉴定,表明它是一个小麦-黑麦(Triticumaestivum-Secalecereale)1BL/1RS异易位系。
短句来源
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  scar marker
Polymorphism of one SCAR marker differed from that revealed with random primers.
      
The transition from complex RAPD spectra to amplification of a particular SCAR marker substantially facilitates analysis of large samples for the presence or absence of the examined fragment.
      
Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean
      
A co-dominant SCAR marker SCS3620 >amp;amp; 580 has been developed based on the sequences.
      
The segregation of SCS3620 >amp;amp; 580 is similar to that of RAPD marker OPS03620 >amp;amp; 580-Significant polymorphism has been shown between resistant and susceptible genotypes when 62 soybean genotypes were surveyed for the SCAR marker.
      
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  scar markers
Analysis of Specific RAPD and ISSR Fragments in Maize (Zea mays L.) Somaclones and Development of SCAR Markers on Their Basis
      
Specific polymorphism revealed with random primers was completely confirmed using five SCAR markers.
      
Two RAPD markers that were closely associated with the chi115 gene were converted into the sequence characterized amplified region (SCAR) markers.
      
Development and Study of SCAR Markers in Pea (Pisum sativum L.)
      
Of the 14 STS markers obtained from 14 polymorphic RAPD fragments, 12 were polymorphic, i.e., they are SCAR markers that can be used in genetic analysis.
      
更多          
  a scar marker
A RAPD marker, ROH20450, linked to the Mesoamerican Co-2 anthracnose resistance gene, was previously transformed into a SCAR marker, SCH20.
      
These primers generated, on the same genotypes used for RAPD analysis, a SCAR marker 390?bp in length and male-specific.
      
One of the alliinase genes and a SCAR marker linked to the disease resistance gene to downy mildew are present on this map.
      
A fragment of the same size as that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by AccI exhibited polymorphism in fragment length between the two lines.
      
Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selecti
      
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Seeds of mung bean Zhong Lü 1 were carried into space in 1994 by a recoverable satellite. After three years ground cultivation and selection, a long pod mutant line was obtained. The pod length of this mutant line ranged from 13 to 17cm and averaged 16cm. The seed setting number in each pod ranged from 15 to 19,averaged 17. By RAPD (Randomly Amplified Polymorphic DNA) analysis on this mutant line and its original CK line, 3 of total 100 10 mer Operon primers amplified polymorphic products were selected....

Seeds of mung bean Zhong Lü 1 were carried into space in 1994 by a recoverable satellite. After three years ground cultivation and selection, a long pod mutant line was obtained. The pod length of this mutant line ranged from 13 to 17cm and averaged 16cm. The seed setting number in each pod ranged from 15 to 19,averaged 17. By RAPD (Randomly Amplified Polymorphic DNA) analysis on this mutant line and its original CK line, 3 of total 100 10 mer Operon primers amplified polymorphic products were selected. The molecular weight of the specific amplified products was 1.4 kb, 1.0 kb, 2.0 kb respectively, therefore they were named as OPZ 13 1400 , OPY 07 1000 and OPY 04 2000 respectively. So far, the cloning of OPZ 13 1400 and OPY 07 1000 has completed. One of them, OPY 07 1000 was transferred further into stable SCAR marker. Three samples with a same phenotype and with about the same pod length selected from this mutant line showed no polymorphism in their RAPD pattern. But they differed from the original CK line with the same polymorphism. These results indicated that the long pod mutant line may be close to be stable in genetics.

中绿1号绿豆种子于1994年经返回式卫星搭载后,经过3年的地面种植和筛选得到了基本稳定的长荚型突变系,该突变系荚长16cm左右,每荚种子粒数为15~19粒。通过对3个形态和荚长都基本稳定的突变系和原始对照品系之间的RAPD分析,从100个10-merOperon引物中筛选出3个能产生稳定的遗传多态性的引物,其特异扩增产物分子量依次为1.4kb、1kb、2kb,因此分别命名为OPZ-131400、OPY-071000、OPY-042000。OPZ-131400和OPY-071000的克隆和鉴定工作已经完成,其中OPY-071000转换成了稳定的SCAR标记。3个突变系进行RAPD分析,彼此之间没有发现扩增产物的差异,它们与原始对照之间却有着共同的差异,表明长荚型突变品系在DNA分子水平上已接近同源。

Based on sequencing analyses of a codominant SCAR marker SCW 05 660 tightly linked to SMV resistance gene Rsa, two pairs of specific SCAR primers were designed and synthesized to amplify genomic DNA from 30 cultivated soybean accessions. Fingerprinting results showed that shorter fragments S 5 600 and S 05 660 were typical bands in disease resistance varieties, while longer fragments S 5 1000 and S 5 1600 were typical bands in susceptible accessions. Southern blot hybridization...

Based on sequencing analyses of a codominant SCAR marker SCW 05 660 tightly linked to SMV resistance gene Rsa, two pairs of specific SCAR primers were designed and synthesized to amplify genomic DNA from 30 cultivated soybean accessions. Fingerprinting results showed that shorter fragments S 5 600 and S 05 660 were typical bands in disease resistance varieties, while longer fragments S 5 1000 and S 5 1600 were typical bands in susceptible accessions. Southern blot hybridization results showed that these fragments generated by the two pairs of SCAR primers were homologous.

依据与SMV(SoybeanMosaicVirus)抗性基因紧密连锁的共显性SCAR标记SCW—05660的序列测定结果,合成了两对SCAR(SequenceCharacterizedAmplifiedRe-gions)标记特异引物,对30个栽培大豆品种进行了指纹图谱分析。结果表明,较短的片段S—5600和S—05660是抗病品种的特征性条带;而较长的片段S—51000和S—51600是感病品种的特征性条带。Southern杂交结果表明,这两对引物扩增出的条带具有同源性。

RAPD marker (OPH17(1400)) and SCARmarkers (SCAR1400 and SCAR(1265)) as well as fluorescence in situ hybridization (FISH) technique were used to detect wheat powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/ 6VS) in different genetic background of wheat breeding program. Among a total of 372 RAPD amplification reactions, 28 reastions (7.53%) yield no ampliflcation products, and the amplification products of 21 reactions (5.64%) were difficult to interpret the presence or absence of the...

RAPD marker (OPH17(1400)) and SCARmarkers (SCAR1400 and SCAR(1265)) as well as fluorescence in situ hybridization (FISH) technique were used to detect wheat powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/ 6VS) in different genetic background of wheat breeding program. Among a total of 372 RAPD amplification reactions, 28 reastions (7.53%) yield no ampliflcation products, and the amplification products of 21 reactions (5.64%) were difficult to interpret the presence or absence of the OPH17(1400), indicating that RAPD marker OPH17(1400) is less reproducible and reliable, so that its usage in breeding program is limited. However, SCAR markers, SCAR(1400) and SCAR(1265), were amplified in all 488 PCR reactions, suggesting that SCAR markers are highly reproducible and reliable, and can be used in breeding Program. The specific SCAR(1265) made it possible to screen a large number of genotypes reliably and rapidly in practical breeding programs for the presence/absence detection between the resistant and susceptible plants. After backcrossed with common wheat lines for several bines, no recombination between the 6VS chromosome of Haynaldia villosa and any of the wheat chromosomes was detected by using FISH.

利用小麦抗白粉病基因Pm21的RAPD标记(OPH17(1400))、SCAR标记(SCAR(1400)和SCAR(1265))和荧光原位杂交技术(FISH)对小麦抗病育种材料中的抗白粉病Pm21基因进行了分子鉴定和标记辅助选择。利用随机引物OPH17进行RAPD分析结果表明,在3~5次重复共372次RAPD扩增中,有28次(7.53%)未获得扩增产物,有21次(5.64%)扩增结果难以判断目标片段OPH17(1400)的有无,说明RAPD标记检测结果的可靠性和重现性较差,在育种中应用有一定局限性。而在利用SCAR标记共488次PCR扩增中,均可以扩增出与Pm21基因连锁的多态性SCAR(1400)或SCAR(1265)目标片段,说明SCAR标记是稳定、准确、可靠的DNA分子标记,可应用于育种群体中Pm21基因的分子鉴定和标记辅助选择。利用RAPD和SCAR标记对具有不同遗传背景的"滚动式加代回交转育"抗白粉病育种群体中抗病基因Pm21分子标记检测结果表明,DNA分子标记与植株的白粉病抗性表现一致,并在此基础上进行了标记辅助的向农艺亲本的回交转育。荧光原位杂交结...

利用小麦抗白粉病基因Pm21的RAPD标记(OPH17(1400))、SCAR标记(SCAR(1400)和SCAR(1265))和荧光原位杂交技术(FISH)对小麦抗病育种材料中的抗白粉病Pm21基因进行了分子鉴定和标记辅助选择。利用随机引物OPH17进行RAPD分析结果表明,在3~5次重复共372次RAPD扩增中,有28次(7.53%)未获得扩增产物,有21次(5.64%)扩增结果难以判断目标片段OPH17(1400)的有无,说明RAPD标记检测结果的可靠性和重现性较差,在育种中应用有一定局限性。而在利用SCAR标记共488次PCR扩增中,均可以扩增出与Pm21基因连锁的多态性SCAR(1400)或SCAR(1265)目标片段,说明SCAR标记是稳定、准确、可靠的DNA分子标记,可应用于育种群体中Pm21基因的分子鉴定和标记辅助选择。利用RAPD和SCAR标记对具有不同遗传背景的"滚动式加代回交转育"抗白粉病育种群体中抗病基因Pm21分子标记检测结果表明,DNA分子标记与植株的白粉病抗性表现一致,并在此基础上进行了标记辅助的向农艺亲本的回交转育。荧光原位杂交结果表明,经多代回文改良,未发现簇毛麦6VS染色体臂与普通小麦染色体的重组。

 
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