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gag蛋白
相关语句
  gag protein
     Immunity strategy induced by HIV-1 CN54 Gag protein combined with DNA vaccine
     HIV-1 CN54 Gag蛋白与DNA疫苗联合免疫策略研究
短句来源
     Randomization of P2/NC protease cleavage site of HIV-1 Gag protein and and its phage display
     HIV-1 Gag蛋白P2/NC蛋白酶切割位点序列的随机化及噬菌体展示
短句来源
     Recombinant, Expression and Application of HIV-1 Gag Protein
     HIV-1 Gag蛋白的重组、表达与应用
短句来源
     Expression and Purification of HIV 2 gag Protein in E coli
     HIV-2 gag蛋白在大肠杆菌中的表达纯化
短句来源
     Immunogenicity of yeast expressed Chinese HIV-1 Gag protein (CN54, B'/C recombinant) in mice
     酵母表达的HIV-1中国株CN54 Gag蛋白的免疫原性研究
短句来源
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  “gag蛋白”译为未确定词的双语例句
     Expression of HIV-2 gag gene in E.coli with a plasmid containing P_RP_L promoter
     应用P_RP_L串联启动子表达HIV-2gag蛋白
短句来源
     Gag expression was detectable in cultured cells infected with both rAAV2-gag and rAAV1-gag.
     用Western blot法和间接免疫荧光法检测rAAV1-gag和rAAV2-gag体外转染的293细胞,均可检测到HIV-1 Gag蛋白的表达;
短句来源
     After 3 strains of HIV-1 Gag gene recombinant vaccinia virus were constructed,14.7,6.0 and 4.5μg/106 cells/20h of Gag proteins were expressed in the cells infected with the 3 strains respectively.
     在构建3株人Ⅰ型免疫缺陷病毒(HIV-1)Gag基因重组痘苗病毒的基础上,实现了Gag蛋白的高效表达,3株重组病毒在感染细胞中表达量分别为14.7、6.0和4.5μg/106细胞/20h,接近杆状病毒的表达水平。
短句来源
     Expression in Pichia pastoris, fermentation and purification of HIV-1 CN54 Gag antigen
     HIV-1中国株CN54 Gag蛋白在毕赤酵母中的表达纯化和鉴定
短句来源
     Results There were 4 conservative antigen epitopes existed in HIV-1 gag P24 region. More diversity of antigen epitopes in gag P17 region than that in P24 was observed.
     结果在H IV-1 gag蛋白P 24编码区,有4个抗原表位相当保守,且P 17部分的抗原表位的变异率高于P 24部分。
短句来源
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  相似匹配句对
     Expression and Purification of HIV 2 gag Protein in E coli
     HIV-2 gag蛋白在大肠杆菌中的表达纯化
短句来源
     Objective To express HIV-1 gag protein in E.
     目的表达HIV-1型gag基因蛋白
短句来源
     proteinuria(-);
     尿蛋白(-);
短句来源
     RADIOIMMUNOASSAY OF F PROTEIN
     F蛋白的放射免疫分析
短句来源
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  gag protein
Retrovirus gag protein p30 in the islets of non-obese diabetic mice: relevance for pathogenesis of diabetes mellitus
      
The disease develops in response to infection with a defective virus encoding a Pr60gag protein that is myristylated and binds to cell membranes.
      
The p110gag-myc protein coded for by the retrovirus MC29 was purified 3,000-fold from MC29-Q8 transformed cells by immuno-affinity chromatography using IgG specific for the N-terminal region of the gag protein.
      
coli, Tnt1-94 polyprotein is cleaved off by the element-encoded protease to release a Gag protein with an apparent molecular mass of 37 kDa that forms high-density aggregates.
      
The test system for the detection of anti-HIV antibodies included peroxidase-conjugated HIV-specific recombinant Gag protein fragments (epitopes of p24 and p17 proteins), Env-1 (epitopes of p41 and p120 proteins), and Env-2 (p36 epitopes).
      
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A sensitive and specific sandwish ELISA was established for identification of McAbs against HIV. Three stable hybridoma cell lines, secreting McAbs reacted with HIV protein(P24), were obtained. The titers of McAbs secreted by hybridoma cells in culture were about 1:6400 and as high as 1:2 430000 in the inoculated mice ascites.

我们用HIV抗体阳性者血浆包被聚苯乙烯条,加入纯化的HIV组织培养抗原,通过夹心ELISA法,筛选出3株能分泌抗HIV单克隆抗体杂交瘤细胞,传代稳定。培养上清经Western blot染色鉴定含有抗-HIV gag蛋白。夹心ELISA法测得杂交瘤细胞培养上清中单克隆抗体滴度达到1∶6400,接种BALB/C小鼠获腹水抗体效价为1∶2430000。实验表明,用此单克隆抗体检测HIV病人血清抗体敏感、特异、稳定、重复性好,是一种良好的试剂。

By inserting the gag gene at the downstream of Cowpox A type inclusion body (ATI) promoter or a hybrid promoter composed of ATI and 16 tandemly repeated mutant early p7.5 promoter, recombinant vaccinina viruses containing region of the gag ORF and different truncated domains of long terminal repeats (LTR) of human immunodeficiency virus type 1 (HIV 1) were constructed. Indirect immunofluorescence assay (IFA), Western blot and immunoenzyme assay (IEA) showed that all the recombinant vaccinia viruses expressed...

By inserting the gag gene at the downstream of Cowpox A type inclusion body (ATI) promoter or a hybrid promoter composed of ATI and 16 tandemly repeated mutant early p7.5 promoter, recombinant vaccinina viruses containing region of the gag ORF and different truncated domains of long terminal repeats (LTR) of human immunodeficiency virus type 1 (HIV 1) were constructed. Indirect immunofluorescence assay (IFA), Western blot and immunoenzyme assay (IEA) showed that all the recombinant vaccinia viruses expressed the Gag protein. Among them, v16LG△2,containing TAR and the downstream sequence, produced the highest level of Gag protein. Densitometric analysis of the stained gels showed that 5.192% Gag protein was contained in the lysate of BHK21 cell infected with v16LG△2, i. e. 10 6 cells infected with the produced at least 14.7 μg Gag protein. This expression efficiency reached the level of foreign protein produced in recombinant baculovirus. Observation with electron microscope revealed that the recombinant Gag protein assembled into virus like particles and buded from cell plasma membrance. Recombinant vaccinia viruses containing gag gene were able to elicit specific antibody against HIV 1 in immunized mice. The present data may be useful to the development of AIDS vaccine.

Gag蛋白是人Ⅰ型免疫缺陷病毒(HIV-1)重要的结构蛋白,可诱导机体产生体液和细胞免疫应答。本试验将HIV-1不同长度的长末端重复序列(LTR)和全长的gagORF置于痘病毒启动子控制下,经过同源重组、红细胞吸附试验筛选和间接免疫荧光试验鉴定,分离了6株重组痘苗病毒。免疫印迹(Westernblot)和免疫酶试验检测表明,6株病毒均能表达gag基因,其中以v16LG△2的Gag蛋白表达量最高,占细胞裂解物上清的5.19%,相当于14.7μg/106细胞,接近杆状病毒的表达产量。重组蛋白主要为p55gag蛋白前体,但也有一部分被加工,生成成熟蛋白p24gag、p17gag及其中间蛋白。表达的重组蛋白可形成病毒样粒子(VLP),并可诱导小鼠产生抗Gag蛋白抗体

Four eucaryotic expression plasmids containing cDNA of HIV2 gag gene under the control of different hybrid promoters of pox virus and 10 or 16 tandemly repeated mutant early P7.5 were constructed, and transfected into BHK21 cells which were infected by wild type vaccinia virus. The recombinant rate of 0.1%, positive recombinant rate of 25%100% and the syncytium formation to great extent with flank sequence of HA gene of vaccinia virus were observed. Indirect immunofluorescence assay (IFA) and Western blot...

Four eucaryotic expression plasmids containing cDNA of HIV2 gag gene under the control of different hybrid promoters of pox virus and 10 or 16 tandemly repeated mutant early P7.5 were constructed, and transfected into BHK21 cells which were infected by wild type vaccinia virus. The recombinant rate of 0.1%, positive recombinant rate of 25%100% and the syncytium formation to great extent with flank sequence of HA gene of vaccinia virus were observed. Indirect immunofluorescence assay (IFA) and Western blot showed that all the recombinant vaccinia viruses expressed the Gag protein which can specifically response to HIV2 positive serum, and induce HIV2 Gag specific antibody in mice.

以痘苗病毒HA基因为侧翼,将HIV-2gag基因部分序列(简称G1)和全部序列(简称G2)分别插入牛痘病毒A型包涵体(ATI)和串联10个(与HA基因反向)或16个(与HA基因同向)痘苗病毒P7.5组成的复合型启动子下游,构建了4个重组痘苗病毒表达载体质粒。经脂质体转染、鸡红细胞吸附试验筛选和免疫荧光鉴定,获得4株能稳定表达目的蛋白的重组痘苗病毒。实验结果表明,以HA基因为侧翼构建重组病毒的重组率约为0.1%,阳性重组率为25%~100%。实验中还发现,以HA基因为侧翼的重组痘苗病毒能够引起细胞融合,其可作为筛选重组痘苗病毒的另一种标志。Westernblot结果表明,表达的Gag蛋白能被HIV-2血清中的特异性抗体所识别,并能诱导小鼠产生抗HIV-2Gag抗体。

 
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