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辐射细胞学
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  radiation cytology
     PCC TECHNIQUE AND ITS APPLICATION IN RADIATION CYTOLOGY
     PCC技术及其在辐射细胞学上的应用
短句来源
     Practical applications for PCC in radiation cytology are:1. to visualize chromosome break in nondividing cells;
     PCC技术在辐射细胞学上的应用主要有三个方面:①作为一种较敏感的染色体损伤检测法,它能检测出M期无法察觉的或M期已经消失的间期染色体损伤.
短句来源
  radiation cytological
     Radiation cytological effects on different types of cultivated rice by gamma-rays
     γ射线对不同类型栽培稻的辐射细胞学效应
短句来源
     To study the radiation cytological effect on pollen grain function, fresh pollen samples shed from Vicia faba were exposed to 60 Co γ ray in doses ranging from 0 to 837 Gy,and immediately permitted to germinate in vitro.
     用60Coγ射线0~837Gy辐照蚕豆成熟花粉,观察在人工培养下萌发花粉的显微、亚显微辐射细胞学效应。
短句来源
  “辐射细胞学”译为未确定词的双语例句
     THE BIOLOGICAL EFFECTS OF RADIATING SEED UNDER EXTREMELY LOW TEMPRETURE——Ⅳ. The Observation of Cytological Under Radiation
     在极低温下照射种子的生物学效应——Ⅳ.对辐射细胞学效应的观察
短句来源
  相似匹配句对
     PCC TECHNIQUE AND ITS APPLICATION IN RADIATION CYTOLOGY
     PCC技术及其在辐射细胞学上的应用
短句来源
     Applications of laser irradiation in cytology and genetics
     激光辐射细胞学遗传学领域的应用
短句来源
     CHANNELING RADIATION
     沟道辐射
短句来源
     On radiation from printed circuits
     印制电路的辐射
短句来源
     A SURVEY OF CYTOLOGY IN THE PTERIDOPHYTA
     蕨类植物细胞学概说
短句来源
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  radiation cytology
The final section provides a look towards the future and makes recommendations including suggestions for technological innovations that would enhance the field of radiation cytology.
      


Fusion of a mitotic cell with a cell in interphase results in a precocious attempt of the interphase nucleus to enter mitosis. The chromatin of the in. terphase nucleus condenses into chromosomelike structures. This technique is called Premature Chromosome Condensation (PCC). The favorable conditions of cell fusion include high calcium concentration, high pH of the medium and the application of Polyethynene Glycol (PEG). For example,our studies show that the optimum pH is between 8-8.2% and the optimum concentration...

Fusion of a mitotic cell with a cell in interphase results in a precocious attempt of the interphase nucleus to enter mitosis. The chromatin of the in. terphase nucleus condenses into chromosomelike structures. This technique is called Premature Chromosome Condensation (PCC). The favorable conditions of cell fusion include high calcium concentration, high pH of the medium and the application of Polyethynene Glycol (PEG). For example,our studies show that the optimum pH is between 8-8.2% and the optimum concentration of CaCl2 is o.4mg/ml.Practical applications for PCC in radiation cytology are:1. to visualize chromosome break in nondividing cells;2. to facilitate a mere detailed analysis of the structure of banding Patterns after certain forms of chromosome; and3. to monitor the repair of chromosome damage.

用M期细胞同间期细胞融合以诱导间期核染色质提前浓集的技术叫做PCC技术.PCC技术可以诱导间期细胞出现各种不同时相的具有一定形态的PC染色体.PC染色体诱导成功的先决条件是细胞融合率高.提高细胞融合率决定于融合剂的选择、Ca离子浓度和pH值高低.我们过去的试验表明:选用PEG作融合剂并在培养液中加入0.2—0.4mg/ml氯化钙,pH调到8—8.2时有较高的PCC诱导率.PCC技术在辐射细胞学上的应用主要有三个方面:①作为一种较敏感的染色体损伤检测法,它能检测出M期无法察觉的或M期已经消失的间期染色体损伤.②、通过制作更精细的染色体谱带以正确定位染色体的断裂点和突变基因的座位.③.用以直接观察非S期修复合成的细胞学图象.

The dried seeds of vicia faba are exposed to ~60Co Y-rays of the same dosagerate and different dosages and the changes in the root tip cells are recorded, The results are as follows; 1。The dosage of radiation is inversely proportional to the index of cell division(r=-0.96)。 2 .Nuclear and chromosomal aberrations are observed in all materials treated by various dosages of radiation。The total frequency of aberrations is related to the dosage of radiation, and a certain threshold exists...

The dried seeds of vicia faba are exposed to ~60Co Y-rays of the same dosagerate and different dosages and the changes in the root tip cells are recorded, The results are as follows; 1。The dosage of radiation is inversely proportional to the index of cell division(r=-0.96)。 2 .Nuclear and chromosomal aberrations are observed in all materials treated by various dosages of radiation。The total frequency of aberrations is related to the dosage of radiation, and a certain threshold exists in it。 3 .It is observd by means of BSG banding technique that most of the fragments come from the terminals of long arms of M chromosomes and that Mchromosomes in which breakage occurs often form chromosomal rings with Chromosome No,6。 From the results mentioned above we may conclude=(1)Detemination of micronuclei and fragments may possibly be regarded as an index of cytological effect of radiation; (2)The chromosomal banding technique contributes to a qualitative etermi-nation of the sources of fragments, whether the dosage threshold is the optimum mutagenic dosage remains a question for further;}study,

本试验采用~(60)Coγ射线同一剂量率(131伦/分)、不同剂量(5000伦、7000伦、10000伦)处理蚕豆干种子,观察根尖细胞变异情况,得到如下结果。 1.辐照剂量与细胞分裂指数呈负相关,即随辐照剂量的增加,细胞分裂指数依次递减(r=-0.96); 2.总的畸变频率与辐照剂量有关,且存在着某一闪值。即总的畸变频率随辐照剂量增加而增加,达到某一阈值(7000伦)后,则随辐照剂量增加而递减,表现出明显的剂量效应。但又随畸变类型不同而有所差异; 3.在各辐照剂量中,都普遍看到核畸变和染色体畸变的出现。在核畸变方面,以7000伦的变异频率为最高,达3.32%,占总畸变率的52.28%,在核畸变中,又以微核出现最多,占整个核畸变的85.51%,在染色体畸变方面也以7000伦为最高,达3.03%,占总畸变率的47.72%,在染色体畸变中,又以断片出现较多,占整个染色体畸变的30.66%; 4.采用BSG法进行染色体显带发现有断片大多来自M染色体的长臂末端,以及发生断裂的M染色体常与第6号染色体一起形成染色体环。由此,可以得出以下结论:1)微核和断片的测定有可能作为辐射细胞学效应的一个指标;2)利用染色...

本试验采用~(60)Coγ射线同一剂量率(131伦/分)、不同剂量(5000伦、7000伦、10000伦)处理蚕豆干种子,观察根尖细胞变异情况,得到如下结果。 1.辐照剂量与细胞分裂指数呈负相关,即随辐照剂量的增加,细胞分裂指数依次递减(r=-0.96); 2.总的畸变频率与辐照剂量有关,且存在着某一闪值。即总的畸变频率随辐照剂量增加而增加,达到某一阈值(7000伦)后,则随辐照剂量增加而递减,表现出明显的剂量效应。但又随畸变类型不同而有所差异; 3.在各辐照剂量中,都普遍看到核畸变和染色体畸变的出现。在核畸变方面,以7000伦的变异频率为最高,达3.32%,占总畸变率的52.28%,在核畸变中,又以微核出现最多,占整个核畸变的85.51%,在染色体畸变方面也以7000伦为最高,达3.03%,占总畸变率的47.72%,在染色体畸变中,又以断片出现较多,占整个染色体畸变的30.66%; 4.采用BSG法进行染色体显带发现有断片大多来自M染色体的长臂末端,以及发生断裂的M染色体常与第6号染色体一起形成染色体环。由此,可以得出以下结论:1)微核和断片的测定有可能作为辐射细胞学效应的一个指标;2)利用染色体显带有助于定性地测定断片的来源。至于剂量阈值是否就是最佳诱变剂量的问题,有待进一步研究。

After dry seeds of twelve cultivars belonging to different types of cultivated rice were treated bt ~(137) Cs Gamma—rays, their dose—effects of mitotic index and micronucleus cell rate in root tip cells were fitted by linear regression equations.As the dose increased, the mitotic index decreased but the micronucleus cell rate increased.Among the different types of rice, the differences of radiation cytological effects were not significant but significant among the cultivars.It is necessary to consider the radio—sensetivity...

After dry seeds of twelve cultivars belonging to different types of cultivated rice were treated bt ~(137) Cs Gamma—rays, their dose—effects of mitotic index and micronucleus cell rate in root tip cells were fitted by linear regression equations.As the dose increased, the mitotic index decreased but the micronucleus cell rate increased.Among the different types of rice, the differences of radiation cytological effects were not significant but significant among the cultivars.It is necessary to consider the radio—sensetivity of species when selecting the material on radiation breeding.

12个不同类型栽培稻品种的干种子经120-480Gy~(137)Csγ射线辐照后,根尖细胞有丝分裂指数(MI)和微核细胞率分别与照射剂量呈显著相关。随着辐照剂量的增加,有丝分裂指数下降。微核细胞率增加。协方差分析表明,不同类型栽培稻的辐射细胞学效应差异不显著,而品种间差异显著,因此在辐射育种选择亲代材料时,应考虑其辐射敏感性。

 
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