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直接重复
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  direct repeat
     a CAAT box at 1886-1889, 225bp before the start codon; and a G box at 1911-1919 in the fragment, a 29 bp direct repeat unit was also found at 1251-1279,1295-1323. The sequence had the character of a plant promoter.
     经初步分析,在该片段的 19631、距基因起始密码子 148hp处有一个 IAIA盒,在该片段的 1886q、距起始密码子 225hp处有一个 CAAT盒,在 19lllgl6bp、CAAT盒和 IAIA盒中间有一个G盒序列,在1251-1279、1295-1323包含一个29碱基的直接重复序列,具备植物启动子的基本特征。
短句来源
     A new method is described for labelling of oligonucleotide piobe of HBV. The oligonucleotide is a 21-nucletide sequence 5' -(CTTCGCTTCACCTCTGCACGT). It is complementary to a region near the end of single-stranded gap of HBV and contains the direct repeat (DR) sequence (underlined). The oligonucleotide was labelled with Biotin-11-dUTP by TdT.
     本文首次报告用3’—末端核苷酸转移酶将生物素—11—dUTP标记到人工合成的21寡核苷酸上,该d(NMP)_(21)与HBV DNA长链缺口区附近的序列互补,并含HBV DNA的直接重复序列(Direct Repeat)。
短句来源
     However,defected HIV 1 genomes of variable size were extensively detected in PBMCs of 9/10 HIV 1 infected persons. Using oligonucleotide probes the frequency of deletions was found to increase with their proximity to the center of the HIV 1 genome. Short direct repeat of three or four nucleotides was present at the deletion junctions in analyzing further detail;
     结果 :经扩增 9.1kb是LD PCR的主要产物 ,但在 10例HIV 1感染者中有 9例PBMCs检测出大小不一、范围较广的缺失HIV 1基因片段 ,经用基因探针杂交 ,发现近HIV 1基因中心部位缺失频率增加 ,缺失结合点常存在 3~ 4个核苷酸短片段直接重复 ,体外培养中HIV 1基因缺损量减少 ,完整和缺失基因的存在与培养中病毒分离的时间密切相关。
短句来源
     The sequence analysis indicated that the integrate sites of prophages 933v and 933w Located at 2966137 and 1330826, respectively. There were 20bp and 7bp direct repeat sequences flanking the prophages 933v and 933w. The flanking repeat sequences are termed as attachment sites (attL and attR).
     序列比较分析表明原噬菌体 933v的整合位点位于O15 7∶H7EDL933基因组中第 2 96 6 137位的相对位置 ,在整合位点两侧有一个 2 0bp的直接重复序列 ,原噬菌体 933w的整合位点位于基因组中第 1330 82 6位的相对位置 ,在整合位点两侧有一个 7bp的直接重复序列。
短句来源
  direct repetitive
     The genetic diversity of Mycobacterium tuberculosis strains in Thailand studied by amplification of DNA segments containing direct repetitive sequences
     应用扩增直接重复序列DNA片段对泰国结核分支杆菌菌株基因差异的研究
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  “直接重复”译为未确定词的双语例句
     (1) the amino acid change in ORF Ⅵ was about 16%, considerably higher than that occured in other coding regions and that observed among the other three isolates; ( 2 ) an insertion of 39 bp at amino-proximal region of ORF Ⅳ, its Sequence was almost the same as that of the target region;
     (1)ORFⅥ编码的氨基酸变化达16%,显著高于其他区域的变化和其他三个分离物之间的差别,(2)在ORFⅣ的近氨基末端有39bp的插入,它与被插入区的序列几乎是直接重复;
短句来源
     There is a hot region (33 3%, 5/15) in basic core promoter where deletion mutation frequently happened.
     在直接重复序列 (DR)Ⅰ上游存有一缺失突变高发区 33 3% (5 15 )。
短句来源
     The catalyst can be reused for more than 8 times.
     催化剂无需处理可直接重复使用8次以上。
短句来源
     IES2 is 41 bp long and flanked by 5'-TA-3' direct repeats.
     IES1和IES2分别长41bp,IES1以GA二核苷酸直接重复为删除信号,IES2以TA二核苷酸直接重复为删除信号。
短句来源
     Labelling of Oligonucleotide of Hepatitis B Virus(HBV)by Terminal Deoxynucleotidyl Transferase(TDT)
     3′-末端标记法制备乙型肝炎病毒(HBV)直接重复序列(DR)的生物素化的寡核苷酸探针
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  相似匹配句对
     ENJOY REPETITION
     喜欢重复
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     kill directly the tumor cells;
     直接杀伤;
短句来源
     and 4) tubes were fertigated on the depth of 40 cm from soil surface (D 40 ).
     4个重复
短句来源
     The catalyst can be reused for more than 8 times.
     催化剂无需处理可直接重复使用8次以上。
短句来源
     Direct Conversion Receiver
     直接变频接收机
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  direct repeat
Binding of GCNF to the consensus element TCA[AG(G/T)TCA]2 (conRE), to the direct repeat DNA element AGGTCAAGGTCA (DR0), and to extended half-sites such as TCAAGGTCA (XRE) has been demonstrated, but no natural GCNF target gene has been identified.
      
The 3' end of atp9-3 gene is flanked by a direct repeat of 42 bp.
      
ISRm31 is 2,803?bp long and has 22-bp-long terminal inverted repeat sequences, 8-bp direct repeat sequences generated by transposition, and three ORFs (A, B, C) coding for proteins of 124, 115, and 541 amino acids, respectively.
      
Furthermore, deletion mapping and mutational analysis of the stbD promoter region were used to identify a degenerated direct repeat motif required for STP induced GusA expression.
      
However, DNA sequence analysis suggested that direct repeat-rich sites could have promoted several recombination events to diversify the two plasmid molecules.
      
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The complete nucleotide sequence of CaMV-XJ genomic DNA has been determined by Maxam-Gilbert chemical cleavage method from a clone pCaMV Cl7 which contains the full-length viral DNA copy.The entire sequence is 8060 base-pair long. There are six major open reading frames ( ORF)or putative genes in CaMV genome using different reading frames.In comparison with other three completely sequenced CaMV isolates, the following marked differences were observed;(1) the amino acid change in ORF Ⅵ was about 16%, considerably...

The complete nucleotide sequence of CaMV-XJ genomic DNA has been determined by Maxam-Gilbert chemical cleavage method from a clone pCaMV Cl7 which contains the full-length viral DNA copy.The entire sequence is 8060 base-pair long. There are six major open reading frames ( ORF)or putative genes in CaMV genome using different reading frames.In comparison with other three completely sequenced CaMV isolates, the following marked differences were observed;(1) the amino acid change in ORF Ⅵ was about 16%, considerably higher than that occured in other coding regions and that observed among the other three isolates; ( 2 ) an insertion of 39 bp at amino-proximal region of ORF Ⅳ, its Sequence was almost the same as that of the target region; ( 3 ) introduction of two in-phase termi-n ation codons in ORF Ⅷ suggested by T.Hohn which made it imposs be to code for a functional protein.

用Maxam-Gilbert化学切断法,从含全长病毒DNA的克隆pCaMV C17中,测定了CaMV-XJ基因组的全部核苷酸序列。CaMV-XJ DNA含有8,060个碱基对。在不同的译读相位中,CaMV基因组含有6个主要ORF或可能的基因,与其他巳作全序列测定的三个CaMV分离物比较,有三个显著的差别;(1)ORFⅥ编码的氨基酸变化达16%,显著高于其他区域的变化和其他三个分离物之间的差别,(2)在ORFⅣ的近氨基末端有39bp的插入,它与被插入区的序列几乎是直接重复;(3)由于核苷酸置换而引入了终止信号,所以T.Hohn[2]推测,可能存在的ORFⅦ不可能编码有功能的蛋白。

A new method is described for labelling of oligonucleotide piobe of HBV. The oligonucleotide is a 21-nucletide sequence 5' -(CTTCGCTTCACCTCTGCACGT). It is complementary to a region near the end of single-stranded gap of HBV and contains the direct repeat (DR) sequence (underlined). The oligonucleotide was labelled with Biotin-11-dUTP by TdT.The results revealed that after 2 hours incubation the labelling efficiency is the highest. The polymerization in the presence of 1 mM Cobalt ion is better than the polymerization...

A new method is described for labelling of oligonucleotide piobe of HBV. The oligonucleotide is a 21-nucletide sequence 5' -(CTTCGCTTCACCTCTGCACGT). It is complementary to a region near the end of single-stranded gap of HBV and contains the direct repeat (DR) sequence (underlined). The oligonucleotide was labelled with Biotin-11-dUTP by TdT.The results revealed that after 2 hours incubation the labelling efficiency is the highest. The polymerization in the presence of 1 mM Cobalt ion is better than the polymerization in the presence of 10 mM Cobalt ion or 10 mM Magnesium ion. The polymerization in the presence of dNTP is better than the polymerization in the absence of dNTP. When Biotin-11-dUTP is replaced by Biotin-7-dATP, the efficiency of labelling decreases.For colour development, we use two methods. The sensitivity of detection by preformed Streptavidin-Al kaline phosphatase complex is higher than the sensitivity of detection by adding the Streptavidin and Biotinated alkaline phospatase in series.Using this Biotinated oligonucleotide probe, we can detect as little as 25 Pg of standard HBV DNA. HBV DNA can also be detected in sera of Hepatitis B patients by dot-blot hybridization.

本文首次报告用3’—末端核苷酸转移酶将生物素—11—dUTP标记到人工合成的21寡核苷酸上,该d(NMP)_(21)与HBV DNA长链缺口区附近的序列互补,并含HBV DNA的直接重复序列(Direct Repeat)。本文对其标记条件、检出方法进行观察比较,找出合适的条件,制成的生物素化d(NMP)_(21)探针可与标准的HBVDNA进行杂交,检测敏感度为25pg。用这种探针可以从乙型肝炎病人血清中检出HBV DNA。

The present experiment was designed to observe the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in infected horse. One horse was infected experimentally with P337V70 strain and showed no clinical signs after being infected at twice with the same virus strain. Seventeen proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were obtained from the buffy coat and liver of the horse through PCR amplification and cloning. Comparative analysis of the sequences...

The present experiment was designed to observe the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in infected horse. One horse was infected experimentally with P337V70 strain and showed no clinical signs after being infected at twice with the same virus strain. Seventeen proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were obtained from the buffy coat and liver of the horse through PCR amplification and cloning. Comparative analysis of the sequences revealed that some sequences contained the nucleotide insertions in the PND region. The insertions might be generated by direct repeat and strand displacement of sequence segment in their PND gene, showing different lenghts.

为确定马传染性贫血病毒(EIAV)envgp90基因的遗传变异特性,本研究应用EIAV日本分离强毒株P337-V70实验感染了1匹健康马,经第2次感染后,实验马未呈现临床症状。gp90基因的核苷酸序列被直接从这匹马的外周血和肝脏所获得的前病毒DNA扩增。通过克隆和测序从这匹马获得了覆盖EIAVgp90基因主要中和抗原功能区(PND)的17个前病毒序列。通过对这些序列的比较分析,发现一些序列在PND功能区含有核苷酸的插入变异。为揭示PND基因中插入基因片段的产生原因,应用计算机软件对这些片段可能的复制和片段的移位进行了推测。结果显示,这些不同长度的基因插入片段可能是由于其PND基因序列片段的直接重复和移位所产生。鉴于本研究所作序列分析的EIAV变异株是从感染马组织直接获得的,而EIAV的基因组结构在慢病毒中较为简单,故可作为感染模型用于慢病毒持续感染机理的研究。

 
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