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骨髓
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  bone marrow
    Effects of Ly49A transfected lymphocytes on experimental graft versus host disease post allogeneic bone marrow transplantation
    转移杀伤细胞抑制性受体Ly49A的淋巴细胞对异基因骨髓移植后移植物抗宿主病的作用
短句来源
    Study on Homing Law of Bone Marrow Cells after Bone Marrow Transplantation in Mice
    小鼠骨髓移植后骨髓细胞回巢规律研究
短句来源
    Study on the Role of Natural Killer Cells in Mouse Allogeneic Bone Marrow Transplantation and Its Mechanism
    自然杀伤细胞在小鼠异基因骨髓移植中作用及其机制的研究
短句来源
    Study of MRI and ~1HMRS on Normal Middle and Distal Femoral Bone Marrow
    正常股骨中下段骨髓MRI和~1HMRS研究
短句来源
    THE THERAPEUTIC EFFECT OF TRANSPLANTATION OF LONG-TERM CULTURED BONE MARROW CELLS ON IRRADIATED SEMIALLOGENEIC MICE
    移植长期培养的骨髓细胞对照射半同种小鼠疗效的初步观察
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    Intramedullary Infusion --Analysis of 32 cases
    骨髓内输液32例分析
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    Observation of survival of donor cells in patients with allo-BMT by PCR-F and PCR-SSCP
    用PCR-F、PCR-SSCP对骨髓移植植入状态的观察
短句来源
    CLINICAL RESEARCH ON TREATMENT OF ACUTE LEUKEMIA WITH AUTOLOGOUS BONEMARROW TRANSPLANTATION
    自体骨髓移植治疗急性白血病临床研究
短句来源
    MECHANISM OF TOLERANCE INDUCED IN ADULT MICEAND ITS APPLICATION TO ALLOGENEIC BONEMARROW TRANSPLANTATION
    成年小鼠的耐受机制及其在异基因骨髓移植中的应用
短句来源
    A Study on DIS 80 for Detection of Engrafment after BMT
    DIS 80位点分析用于骨髓移植存活检定
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  bone marrow
The effects of hematopoietic stem/progenitor cells (HSPCs) expanded in the two step coculture with human bone marrow mesenchymal stem cells (hMSCs) on the hematopoietic reconstruction of irradiated NOD/SCID mice were studied.
      
Effects of malondialdehyde on growth and proliferation of human bone marrow mesenchymal stem cells in vitro
      
Malondialdehyde (MDA) is a well known inducer of carbonyl stress in a variety of human cells, however, its effects on human bone marrow mesenchymal stem cells (hMSCs) have not been documented.
      
In this study, murine mononuclear cells (MNCs) were isolated from bone marrow and cultured in suspension, and then Lin-CD117+ HSCs were isolated by immunomagnetic beads.
      
Cytokines mRNA in bone marrow-derived dendritic cells in asthmatic mouse
      
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Paper radioimmunosorbent test (PRIST) for determination of human serum total IgE and its clinical application were reported. The required alkaline pH could be easily maintained with 5M alkaline potassium phosphate buffer during paper disc activation with cyanogen bromide. The nonspecific adsorption of labeled antibody could be reduced by diluting the antibody with homologous animal serum. The sensitivity of this method was about 0.6IU/ml and reproducibility and specificity identified. The correlation coefficient...

Paper radioimmunosorbent test (PRIST) for determination of human serum total IgE and its clinical application were reported. The required alkaline pH could be easily maintained with 5M alkaline potassium phosphate buffer during paper disc activation with cyanogen bromide. The nonspecific adsorption of labeled antibody could be reduced by diluting the antibody with homologous animal serum. The sensitivity of this method was about 0.6IU/ml and reproducibility and specificity identified. The correlation coefficient for agreement between PRIST and single radiodiffusion method (RSRD) was 0.91 (P<0.001). Results of clinical application revealed that serum IgE levels from donors, umbilical cords, patients with non-IgE multiple myeloma and Artemisia pollenosis were 307.4, 2.69, 43.3 and 506 IU/ml respectively. Authors suggested that this "sandwich" radioimmunoassay was relatively simple, rapid, sensitive and specific. Problems on non-specific adsorption, advantages and disadvantages of this assay were discussed.

本文探讨了PRIST方法的灵敏度、重复性、特异性、与放射单扩散法的相关性及其临床应用。用5M碱性磷酸钾缓冲液维持溴化氰活化纸片时所需的pH,用标记抗体同种动物血清稀释,简化了操作手续。本法灵敏度为0.6IU/ml;与放射单扩散法相关性密切;献血员、脐带血,非IgE型骨髓瘤和蒿属花粉症患者的IgE值(GM)分别为307.4、2.69、43.3和506IU/ml。本法简便、快速、敏感、特异,适用于测定低浓度IgE。

The results of cryopreservation of bone marrow cells by three different techniques were compared. It was shown that by using the mixture of alcohol and dry ice, liquid nitrogen container, or KSL-811 type of freezing rate control equipment, the viability of hemopoietic stem cells either of bone marrow origin or from fetal liver was the best. The rate of fre-ezing was controlled at 0-3℃/min. The thawed bone marrow suspension containing 5 or 10% dimethylsulfoxide was safe to infuse intravenously into animal and...

The results of cryopreservation of bone marrow cells by three different techniques were compared. It was shown that by using the mixture of alcohol and dry ice, liquid nitrogen container, or KSL-811 type of freezing rate control equipment, the viability of hemopoietic stem cells either of bone marrow origin or from fetal liver was the best. The rate of fre-ezing was controlled at 0-3℃/min. The thawed bone marrow suspension containing 5 or 10% dimethylsulfoxide was safe to infuse intravenously into animal and man without inducing harmful side effects.

本文介绍了酒精加干冰、半自动液氨生物容器降温装置和KSL-811型可控速率冷冻装置3种骨髓超低温保存技术。实验与应用结果表明,冷冻速度在3℃/min以内均可保持造血干细胞活力,将含10%和5%二甲基亚砜的骨髓细胞悬液直接静脉输注给动物和人,未见明显的毒副作用。

This paper introduces a method of preparing fetal liver cell suspension, it is simple and convenient, much cheaper and safer. In addition, we observed the total number of nucleated cells and CFU-C of fetal liver of various months; the comparison of CFU-C yield between normal marrow and the suspension; the activity of cells in suspension after short-term preservation; the cellular composition of fetal liver; the clinical transfusion of suspension etc. Finally., the method of preparing fetal liver cell suspension...

This paper introduces a method of preparing fetal liver cell suspension, it is simple and convenient, much cheaper and safer. In addition, we observed the total number of nucleated cells and CFU-C of fetal liver of various months; the comparison of CFU-C yield between normal marrow and the suspension; the activity of cells in suspension after short-term preservation; the cellular composition of fetal liver; the clinical transfusion of suspension etc. Finally., the method of preparing fetal liver cell suspension and the number of fetal liver needed when fetal liver transplantation are discussed.

本文介绍了胎肝细胞悬液的制备方法,此法简便、成本低廉、使用安全。此外,我们对不同月龄胎肝所含有核细胞总数、CFU-C总数、正常骨髓与胎肝细胞悬液CFU-C产率的比较、胎肝细胞悬液短时保存后的活性、胎肝的细胞组成、临床输注等进行了观察,并对胎肝细胞悬液的制备方法和移植时所需要的胎肝数进行了讨论。

 
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