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β-葡萄糖苷酸酶
相关语句
  β-glucuronidase
     [Results] The activity of endogenous β-glucuronidase in the experiment group was (32 800.0±11 713.2 Fishman unit), in the control group was (10 880.0±2 917.5 Fishman unit);
     结果棕色素结石组胆汁内源性β-葡萄糖苷酸酶活性(32800.0±11713.2)Fishman单位,显著高于对照组(10880.0±2917.5)Fishman单位;
短句来源
     Positive selection system has been developed as an efficient alternative by using non-resistance marker genes with biosafety, some of which are sugar metabolic enzyme genes (pmi and xylA), disturbing amino acid metabolic enzyme genes (ak and dapA), green fluorescent protein gene(gfp),β-glucuronidase gene(gus), ribitol operon(rtl), chlorophyllous biosynthesis enzyme gene (hemL).
     目前解决的有效途径是发展正向选择系统,使用非抗性的生物安全标记基因,主要包括糖类代谢酶基因(pmi和xylA)、干扰氨基酸代谢酶基因(ak和dapA)、绿色荧光蛋白基因(gfp)、β-葡萄糖苷酸酶基因(gus)、核糖醇操纵子(rtl)和叶绿素生物合成基因(hemL)等。
短句来源
     Directed Evolution of High Activity β-Glucuronidase by DNA Shuffling and Screening
     通过DNA改组技术获得高活性β-葡萄糖苷酸酶
短句来源
     In this study,β-glucuronidase(GUS) were transmitted into potato cells.
     本研究还利用 β -葡萄糖苷酸酶 (GUS)基因转化马铃薯细胞。
短句来源
     [Objective] To investigate the effect of lipopolysaccharide(LPS) on β-glucuronidase(β-GD) activity in human peripheral blood mononuclear cells(PBMC),and discuss a kind of mechanism of cholelithiasis pathogenesis.
     目的研究内毒素(LPS)对正常人外周血单核细胞(PBMC)产生β-葡萄糖苷酸酶(β-GD)的影响,探讨胆石症的又一个发病机制。
短句来源
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  beta glucuronidase
     The deduced amino acid sequence demonstrated that 11 amino acid was changed. The Tm value of GUS3 3 is 80℃ and increased by 25℃ above GUS ck (55℃). The researches indicated the feasibility of the molecular evolution of beta glucuronidase in vitro to improve enzymatic thermostability.
     Tm值GUS3 3为 80℃ ,GUS ck为 5 5℃ ,提高了 2 5℃ ,GUS3 3β -葡萄糖苷酸酶热稳定性有了很大的提高 ,GUS3 3酶的最适温度明显提高。
短句来源
  “β-葡萄糖苷酸酶”译为未确定词的双语例句
     The broad-spectrum blast resistance gene Pi9 controlled by promoter CaMv35s was successfully introduced into the calli of five indica cultivars (Fengyuan B, Xiangwanxian13, R996, 527, 1701) based on the Agrobacterium-mediated method. The plasmid is pCAMBIA1301, the T-DNA of pCAMBIA1301 contains hygmycin-resistant gene and gus gene.
     通过农杆菌介导法,将由CaMv35s启动子启动的广谱抗稻瘟病基因Pi9转入5个籼稻品种(丰源B、湘晚籼13号、R996、527、1701)的愈伤组织中,所用质粒为pCAMBIA1301,pCAMBIA1301的T-DNA区含有潮霉素(hygmycin)抗性基因和β-葡萄糖苷酸酶(GUS)基因。
短句来源
     tumefaciens strains on the transformation efficiency were studied. A . tnmefacicns strains harbored the binary vectors, pROA93, pBI121, pBI121 - PI- 1. pBin19 - PI- 2 and PBin19 - PI-4, containing NPTII gene and GUS gene, also the binary vectors pBI121 - VI - 1 ,pBin19 - PI - 2 and pBin19 - PI - 4 containing CpTI gene .
     这些菌株所携带的质粒上均含有卡那霉素抗性基因(NPTⅡ)和β-葡萄糖苷酸酶基因(CUS),另外,质粒pBI121-PI-1、pBin19-PI-2和pBin19-PI-4上还携带有豇豆胰蛋白酶抑制剂基因(CpTI)。
     the enzymes listed below: (1).
     (1)β-葡萄糖苷酸酶;
短句来源
     Analysis of the activity of the promoter of AtSPX3 gene using promoter-β-glucuronidase fusion revealed a predominant expression in the vascular tissues of roots, leaves, and also in the mature microspore.
     用启动子与β-葡萄糖苷酸酶融合的方法来分析AtSPX3基因的启动子的活性发现主要在根中和叶有强烈表达,在花粉粒中也表达。
短句来源
     It was discovered in this experiment that 3 days after inoculation of the ascitic fluid from P_(388) leukemic mice, the positive percentages of AcP and β-Gase of the lymphocytes within the TNC of the recipients were increased significantly, while the positive percentage of ANAE was decreased.
     按种P_(388)白血病患鼠腹水后第3d,小鼠胸腺哺育细胞内的淋巴细胞,酸性磷酸酶和β-葡萄糖苷酸酶阳性率显著增加,而α-醋酸萘酯酶阳性率则下降。
短句来源
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  β-glucuronidase
Their neutrophils, stimulated by phagocytosable particles, were found to secrete increased amounts of alkaline phosphatase, β-glucuronidase, and lysozyme.
      
Bifunctional reporter proteins were constructed to combine Clostridium thermocellum lichenase (LicBM2) with Aequorea victoria green fluorescent protein (GFP) or with Escherichia coli β-glucuronidase (GUS).
      
fluorescens 2137 was marked with the β-glucuronidase gene gusA.
      
melilotiRmM4gustester strain carrying the gene of β-glucuronidase).
      
Variability of β-glucuronidase enzyme activity was shown in original transgenic plants or among progenies of the first generation in both groups.
      
更多          
  β glucuronidase
Integration and expression of the T-DNA encoded marker genes was confirmed by histochemical β glucuronidase assays and Southern-DNA hybridization using primary transformants and S1-progeny.
      
The proportions ofN-acetyl-β-D-glucosaminidase, cathepsin D, and βglucuronidase of the Descemet's membrane-endothelium complex were particularly high: 11.5%, 12.6%, and 12.5% of the whole cornea, respectively.
      
An enhancer trap element, based on the Activator/Dissociator (Ac/Ds) transposons of maize and the β glucuronidase reporter sequence, has been introduced into Hieracium.
      
The purified material was homogeneous on polyacrylamide gel electrophoresis and was sensitive to the action of β glucuronidase and catalase, and partly denatured by urea.
      
Putative calli were subsequently regenerated into cotton plantlets expressing both the kanamycin resistance gene and βglucuronidase (gus) as a reporter gene.
      
  beta glucuronidase
The remaining portions of the bile samples were incubated with beta glucuronidase and reanalyzed for haloperidol and reduced haloperidol.
      
Beside the known acid phosphatase and nonspecific esterase activity two other lysosomal enzymes (beta glucuronidase and arylsulfatase) were found in the renal medullary interstitial cells of the rat.
      
The PTA and PAS stains were abolished by acetylation, restored by saponification, unchanged by methylation and greatly diminished by sulfation or by digestion with beta glucuronidase after oxidation by perchloric acid.
      
The release of neutral proteinase, beta glucuronidase, myeloperoxidase and lactic dehydrogenase as cytoplasmatic marker was measured.
      
The effects of fiber isolated from black gram (Phaseolus mungo) and Coconut (Cocos nucifera) kernel on the metabolic activity of intestinal and fecal beta glucuronidase activity during 1,2-dimethylhydrazine induced colon carcinogenesis were studied.
      
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The general morphology of 15 human embryonic parotid salivary glands (4-6 months) was investigated by H. E, PAS(periodic acid Schiff′s reaction) and AB(Alcian blue) stain, and the histochemical studies of the LDH(lactic dehydrogenase) and its isozymes,NAE(nonspesific acid esterase) and β-GA(β-glucuronidase) were also carried out. The results showed: The tubules and terminal bubls(TB) could be recognized generally in these parotid glands. PAS positive reaction was found in the lumina of tubules and TB, howerer...

The general morphology of 15 human embryonic parotid salivary glands (4-6 months) was investigated by H. E, PAS(periodic acid Schiff′s reaction) and AB(Alcian blue) stain, and the histochemical studies of the LDH(lactic dehydrogenase) and its isozymes,NAE(nonspesific acid esterase) and β-GA(β-glucuronidase) were also carried out. The results showed: The tubules and terminal bubls(TB) could be recognized generally in these parotid glands. PAS positive reaction was found in the lumina of tubules and TB, howerer AB stain was found to be positive only in a few samples; Strong activity of LDH, mainly activity of H type isozyme, was showed in the epithelia of tubules and TB, β-GA and NAE activities were demonstrated in the epithelia of some parotid glands.

本文对15例4~6月的人胚腮腺进行了形态学和酶组织化学[乳酸脱氢酶(LDH)及其同工酶、非特异性酸性酯酶(NAE)和β-葡萄糖苷酸酶(β-GA)]的观察。结果表明:该时期人胚腮腺上皮细胞已分化成许多小管(tubules)和端泡(terminal bulbs,TB),腔内含有中性粘多糖,在部分标本中可见到酸性粘多糖也开始分泌;上皮细胞内含有丰富的LDH,并以H型同工酶为主,部分标本中上皮细胞内开始出现NAE和β-GA。本研究为了解人胚腮腺的形态与功能提供了一些参考资料。

Epitiostanol is extracted and synthesized in China from the waste water of flax making process with the century plant. The LD50 of epitiostanol is 1469 mg/kg, as determined in the mice by intraperitoneal injection, when subacute toxicity was produced in rats by intraperitoneal injection of high (100mg/kg) dose, apparent hyperplasia of the endometrium and inhibition of ovogenesis could be observed. There are apparent inhibition on breast cancer after 300mg/kg of epitiostanol, antiimplantation and antiestrogenic...

Epitiostanol is extracted and synthesized in China from the waste water of flax making process with the century plant. The LD50 of epitiostanol is 1469 mg/kg, as determined in the mice by intraperitoneal injection, when subacute toxicity was produced in rats by intraperitoneal injection of high (100mg/kg) dose, apparent hyperplasia of the endometrium and inhibition of ovogenesis could be observed. There are apparent inhibition on breast cancer after 300mg/kg of epitiostanol, antiimplantation and antiestrogenic effect after 0.5mg of epitiostanol injected subcut ancously observed in mice. When 2.5mg of epitiostanol was given to each rat subcutaneously, progesterone activity on the uterus increased markedly with decreased reactions of β-glucoronidase, acid phosphatase and alkaline phosphatase of the epithelial and glandular cells of the endometum, as proved by histochemical reaction

国产环硫雄醇系由龙舌兰制麻废液中提取合成。小鼠腹腔注射环硫雄醇,急性毒性LD_(50)1469mg/kg。大鼠亚急性毒性,高剂量(100ms/kg)腹腔注射,子宫内膜明显增殖,卵泡成熟受抑制。300mg/kg腹腔注射,对小鼠乳腺癌有明显抑制。0.5mg/只肌肉注射,对小鼠有明显抗雌激素活性和抗着床作用。2.5mg/只肌肉注射,可增强大鼠子宫孕激素活性,组织化学显示子宫内膜上皮和腺细胞的β-葡萄糖苷酸酶、酸性磷酸酶和碱性磷酸酶反应减弱。

Epitiostanol is a anticarcinome drug and have obvious offects of antireproduction function in the femal animal. The aim of this study is to detect the mechanism of antireproduction. 22 Wistar strain female rats were ovariectomized. then, estradial and 18-methyl-nore-thiodronum wereinjected in muscle of the rats by turn in the control group. In experimental animal group, in addition to same treatment as the control group also injedted with Epitiostanol. The deciduoma of the uterus were induced in all animals....

Epitiostanol is a anticarcinome drug and have obvious offects of antireproduction function in the femal animal. The aim of this study is to detect the mechanism of antireproduction. 22 Wistar strain female rats were ovariectomized. then, estradial and 18-methyl-nore-thiodronum wereinjected in muscle of the rats by turn in the control group. In experimental animal group, in addition to same treatment as the control group also injedted with Epitiostanol. The deciduoma of the uterus were induced in all animals. After chemical ireatment, acid phosphotase, alkaline phosphatase, β-GU (glucuronidase) and mucoprotein in the uterus of rats were researched in each histochemical mehtod. The diameter of the uterus were obvious increase, the deciduoma were larger in the experimental group. The epithelium of endometerium, uterine glands, the stromal cells and smooth mluscle, their development and differenation were more well. The value of glucogen in deciduoma were more than the control group. The activity of AGP, ALP, and β-GU in the experimental group had obvious decrease. The authors of this study presumed Epitiostanol was possible to prompt on combine between progesterone and receptor in the ulerus. Inhibited enzymes activity in uterus by Epitiostanol mey be one of the mechanism of antiimplanation and antipregnancy in early. These results were perhaps related through distubance on the metabolism in early embryo and the uterine tissue.

环硫雄醇为抗乳癌药,同时具有明显的抗着床作用。为了探讨该药抗生殖作用机理,本研究采用成年,有生育力的雌性大白鼠22只,摘除卵巢后,对照组先后给予雌二醇和18—甲基炔诺酮,实验组除按对照组给药外,在给18—甲基炔诺酮的同时,给予环硫雄醇。每只动物右侧子宫角穿线诱发蜕膜瘤。先后用药共13天后解剖观察子宫并称重。子宫组织分别作碱性磷酸酶、酸性磷酸酶、β-葡萄糖苷酸酶、多糖类和DNA、RNA等组织化学研究实验组动物子宫明显增粗,蜕膜瘤大,组织观察可见子宫壁厚,内膜上皮、腺体、基质细胞和平滑肌分化较好,组织化学显示碱性磷酸酶、酸性磷酸酶和β-葡萄苷酸酶活性明显减弱。蜕膜瘤细胞的糖元、基质细胞RNA较丰富。对照组三种酶的活性较强,但组织分化较低。本文提出环硫雄醇具有抑制雌激素激发碱性磷酸酶等活性作用,并可能具有促进孕激素与受体结合的效应。其抗着床作用可能与子宫酶活性受到抑制,从而干扰早胚和子宫组织的代谢过程有关。

 
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