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毒细胞
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  virus cell
     We sampled every batch of the seed virus cell cultures and specific batch of the vaccine at regular interval period, and the reovirus contamination was found in one batch of seed virus cell cultures, and was treated properly in time.
     经对每批种子毒细胞培养物及间隔一定批次成品疫苗抽样检查,其中在一批种子毒细胞培养物中发现了呼肠病毒污染,得到及时有效的处理。
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  “毒细胞”译为未确定词的双语例句
     The expression of NKG2D gene in liver issue from 20 chronic hepatitis B(6 with G1 and 2 with G2 of 8 mild hepatitis;9 with G3 and 3 with G4 of 7 moderate and 5 severe hepatitis)and 11 FHB were analyzed by histochemistry S-P method.
     应用免疫组化SP法分析20例慢性乙肝(CHB)(轻度8例,其中G1级6例,G2级2例;中度7例,重度5例,其中G3级9例,G4级3例)、11例FHB肝组织内细胞毒细胞NKG2D的表达差异。
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     The cells were treated with WA atdoses of 156.25,3 12.5,625,1250,and 2500ug/rnl, and the reduction of IVITTwas measured at 24,48,72hours, respectively.
     以156.25、312.5、625、1250、2500ug/ml的TPA浓度分别染毒细胞,在24、48、72小时分别检测MTT的还原量。
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     Conclusion The recombinant retroviral vector pN 2ATH is constructed and PA317/pN 2ATH packaging cell line has high viral titer and TH expression.
     结论 成功地构建了重组逆转录病毒表达载体p N2 ATH,建立了 PA317/p N2 A产毒细胞
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     The transfected PA317/TH and PT67/GDNF cells were selected by the corresponding antibiotics and examined by using RT-PCR and immunohistochemistry.
     经筛选、RT-PCR、免疫组化等鉴定得到产毒细胞 PA3 17/TH和 PT67/GDNF。
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     The PCR product was cloned into pBluescript II SK(+) (pSK) vector to construct the recombinant plasmid designated as SK-PCV2. The PCR primer pair, P5 and P6, amplified the pSK vector and the PCV2 genome without the ORF2 gene (SK-PCV2△ORF2), a fragment of 4023bp, using the plasmid of SK-PCV2 as the template and introduced Nhe I and Sph I restriction enzyme sites;
     通过PCR方法,以引物P1和P2从接毒细胞中扩增到猪Ⅱ型圆环病毒全基因组,克隆入pBluescript Ⅱ SK(+)载体构建质粒SK-PCV2,以其为模板用引物P5和P6扩增其不包含PCV2-ORF2的部分基因(SK-PCV2△ORF2);
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  相似匹配句对
     Cytochalasin B
     细胞B
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     Cytotoxic T Cell
     细胞T细胞
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     SPLINTER CELL
     分裂细胞
短句来源
     2. basal cell;
     基细胞;
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  virus cell
Fluorescent VLPs might be a useful tool for study of papillomavirus virus/cell interaction.
      
Fine genetic mapping of a gene required for Rice yellow mottle virus cell-to-cell movement
      
Identification of a novel human gene homologous to the bovine leukemia virus cell receptor
      
9-O-Acetyl-N-acetyl neuraminic acid (Neu5,9Ac2) structures mediate influenza C virus cell-binding.
      
This unusual orientation is expected to induce the disorganization of the surrounding lipids, which had been suggested as one of the initial events in the virus/cell fusion.
      
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1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The...

1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The method of cultivating hog cholera vaccine virus in rolling bottles around an inclined axis and with deeper culture media(7—8 times more than in the routine method) produced titres in the first and second harvests or in a single harvest(7 days after inoculation) quite similar to those obtained by the routine method.They fitted well the requirements for vaccine production. 3. We also cultivated the LCHV in rolling bottles as cited above but with even much more culture media(8 times more) and additional aerati on. In this case the titres obtained in single harvests(7 days after inoculation)passed most of the 5×10~4RID/1 ml tests also. The data given above suggested that these three methods of cultivation might be applicable in actual vaccine production, with the only consideration that much greater amounts of culture media than used in the routine method of production had to be consumed in these cases. These modifications would much simplify the production of LCHV vaccine.

1、用增加培养液量(3—4倍)旋转法培养猪瘟弱毒细胞疫苗,第一收、第二收病毒收获液的毒价均可达到普通旋转培养法的滴度。二收的绝大多数批次毒价用兔检5万倍通过,符合制苗要求。 2、用深液(培养液量增加7—8倍)倾斜旋转法培养猪瘟弱毒细胞疫苗。第一收与第二收的毒液或培养7天一次全收的毒液,其大多数试验批次毒价均可达到制苗标准。 3、用深液(培养液量增加8倍)倾斜旋转通气法培养猪瘟弱毒细胞疫苗,接毒后培养7天一次全收毒液,大多数批次试验毒价用兔检5万倍通过。符合制苗要求。以上三种培养方法所得资料说明提高猪肾细胞产毒量是明显的,工艺是简便的,在生产上是可行的。对细胞提高繁殖病毒能力的问题进行了某些探索。

This article reported a spontaneous pox infection in rats. Its clinic signs were similar to infectious ectromelia of mice, but mortality rate was lower. The disease was reproduced artificially in mice following inoculation with liver and spleen suspension of the sick rat. And the virus was isolated and a cytopathogenic effect was observed in IBRS, cell line.By phosphotungstic acid negative staining, under the electron microscope viral particles which were morphologically to be similar to poxvirus, 250-350 nm...

This article reported a spontaneous pox infection in rats. Its clinic signs were similar to infectious ectromelia of mice, but mortality rate was lower. The disease was reproduced artificially in mice following inoculation with liver and spleen suspension of the sick rat. And the virus was isolated and a cytopathogenic effect was observed in IBRS, cell line.By phosphotungstic acid negative staining, under the electron microscope viral particles which were morphologically to be similar to poxvirus, 250-350 nm in diameter in the infected IBRS2 cell were photographed.

本文报告一次大鼠的自发性痘病,其症状与小鼠传染性脱脚病相似,但致死率较低。以病鼠肝、脾为接种材料,人工感染小鼠能引起发病,并对IBRS_2传代细胞有致病变作用。取含毒细胞培养液经磷钨酸负染后电镜观察,发现有在形态上与痘病毒相似的病毒粒子,大小为250—350nm。

The study was undertaken to determine pathomorphologically the pathogenicity and immunological effect of attenuated goat pox cellular virus. 22 goats were divided at random into 3 groups: 1 ) Control group of virulent goat pox virus. There were characteristic clinical symptoms and pathological changes of goat pox in all goats inoculated with virulent goat pox virus. A severe inflammatory reaction was found in the skin where the virus was irloculated intradermally. 2 ) Attenuated goat pox virus group. Neither...

The study was undertaken to determine pathomorphologically the pathogenicity and immunological effect of attenuated goat pox cellular virus. 22 goats were divided at random into 3 groups: 1 ) Control group of virulent goat pox virus. There were characteristic clinical symptoms and pathological changes of goat pox in all goats inoculated with virulent goat pox virus. A severe inflammatory reaction was found in the skin where the virus was irloculated intradermally. 2 ) Attenuated goat pox virus group. Neither clinical symptoms nor pathological changes were observed in those goats inoculated with GT4-STV42-58 generations, 66 generations and 85 generations of goat pox cellular virus. Only a mild reaction of the skin inoculated with attenuated virus was found; there were slight proliferation of adventitial cells and infiltration of lymphocytes around the vessels of the dermis. 3 ) Experimental group. Goats immunizing with GT4-STV42-54-85 generations of goat pox cellular virus were challenged by virulent goat pox virus in different periods after immunization. Results showed that all goats were safe without any clinical symptom and pathological change characterized by goat pox,a mild lesion was observed in the skin of individual goat inoculated with virulint virus. The study indicated immunization with GT4-STV42-54-85 generations of goat pox cellular virus could give a good protection. The attenuated goat pox cellar virus could be used as an effective vaccine against goat pox in goats.

为了从病理形态学上明确山羊痘弱毒细胞株的致病性和免疫效应而进行的这项研究,共用试验山羊22只,随机分为三组:强毒对照组、弱毒组和试验组。强毒对照组接种山羊痘强毒后,全部发生山羊痘的典型临床症状和病理变化,且在注毒部皮肤呈现显著的炎症反应。弱毒组接种细胞毒后不见山羊痘的临床症状和病理变化,仅在注毒部皮肤出现轻度反应,即在真皮血管周围见少量的外膜细胞增生和淋巴细胞、浆细胞浸润。 试验组接种细胞毒后不同时期攻击强毒,结果全部获得保护,仅个别羊见注毒局部皮肤有轻度反应。即使个别羊攻毒局部的皮肤有微弱的损伤,但却能使免疫后540天获得坚强的免疫力,与强毒对照组有悬殊的差异。

 
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