The V_H and V_L genes were assembled into scFv (V_H~-) Linker-V_L) genes by the connection with the linker DNA which encodes a flexible peptides (Gly_4Ser)_3 using overlap PCR.
It is suggested that the drug resistance is related with by the newly found alterations, including D294G, S361P, M374V, P386L, H400R, Q474K, and a frameshift.
A prokaryotic expression plasmid of ChIL-18, pGEX-ChIL18, was obtained by subcloning the encoding region of the ChIL-18 mature peptide into pGEX-6P-1. The recombinant ChIL-18 (rChIL-18) was expressed efficiently in pGEX-ChIL18-transformed BL21(DE3)LysS induced by IPTG, the yield was accounted for 32% of the total bacterial protein.
Specifically, pPIC9K-6×his was modified by inserting a DNA fragment coding 6-histidine and 2-glycin in the multiple cloning site of pPIC9K for expressing the fusion protein with a N-terminal polyhistidine tag.
The recombined translocation segments coding for white endosperm are no longer associated with chromosome 7D.
Sequence and diversity of variable gene segments coding for rabbit T-cell receptor beta chains
bv.viciae hybrid strains contained defined DNA segments coding for different combinations ofR.
Comparison of the arrangement of these esterase loci on linkage group VI with the esterase loci on chromosome 8 of the mouse gives additional support for the theory of evolutionary conservation of chromosomal segments coding for mammalian esterases.
By T1-oligonucleotide fingerprinting of RNA segments coding for hemagglutinin (HA), nucleoprotein (NP), and neuraminidase (NA) two genotypes were found.
To investigate the function of this gene in MSR-1, a fur mutant was generated by double crossover with a kanamycin cassette inserted into its coding region.
Its coding gene at the Glu-Dt1 locus was amplified with allele-specific-PCR primers, and the amplified products were cloned and sequenced.
To increase the production of CPY in Saccharomyces cerevisiae we have placed its coding region (PRC1) under control of the strongly regulated yeast GAL1 promoter on multicopy plasmids and introduced the constructs into vpl1 mutant strains.
The enhanced production of clavulanic acid correlated well with the increased total activity of clavaminic acid synthase, a key enzyme in its biosynthesis, and the transcription of cas2, its coding gene.
The FUT2 is rich in polymorphisms that show population-specific patterns in its coding sequence.
Diasonic SPA 1000(10 MHz Duplex)Ultrasound Equipment is connected with computer AST 386,slot Inserted VFG-512 BC graphic panel.VFG two dimensional video frequency graphic process- ing program(C language)convert gray scale into B-colour scale of four section(red→yellow→green→blue colour).The authors consider that B-colour scale sonogram makes up the loss to recognize in gray scale de- tection of eyes microdisease. 109 diseased eves proved by operation or pathology were analyyed.
Diasonic SPA,1000(10MHz Duplex)ultrasound equipment is connected with eoniputer AST386,slot inserted VFG-512 BC graphic panel.VFG two dimensional video frequency graphic processing pro-gram(C language)converted gray scale into B-colour scale into B-colour scale of four section(red→yellow→green→blue colour).The authors consider that B-colour scale sonogram makes up for a loss to recognize in gray scale,for exam-ining microdise-ise of 104 eyes,which proved by operation or pathology.
The seven-scale color-coded ultrasound was used to study salivary gland masses,included 16 pleomorphic adenomas,8 adenolymphomas,7 malignancies and 4 cysts.All were confirmed by surgery and patholof1y. The mean color quantity scale of pleomorphic adenoms was higher than that of adenolymphoma(P<0.01),like that of malignant tumor(P>0.05);the disparity of the color quantity scale was broader than that of adenolymphoma but less than that of malignant tumor.Most of the pleomorphic adenoma and adenolymphoma's border...
The seven-scale color-coded ultrasound was used to study salivary gland masses,included 16 pleomorphic adenomas,8 adenolymphomas,7 malignancies and 4 cysts.All were confirmed by surgery and patholof1y. The mean color quantity scale of pleomorphic adenoms was higher than that of adenolymphoma(P<0.01),like that of malignant tumor(P>0.05);the disparity of the color quantity scale was broader than that of adenolymphoma but less than that of malignant tumor.Most of the pleomorphic adenoma and adenolymphoma's border were sharp and transitional(21/24). Conversely,six of the 7 malignant were mosaic.All but one of the cysts were anechoic and sharp.Border of mass the most valuble fur its diagnosis.
段编码
segments coding
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