The research work including:1. A bovine genomic λ-phage DNA library and a bovine genomic bacterial artificial chromosome (BAC) library were screened with a probe specific for immunoglobulin lambda light chain loci.
2. The BAC clone DNA was digested with eight different restriction enzymes and subjected to Southern Blot analysis by hybridization with a 408bp C_λ probe. Seven positive lanes were presented at the most.
4. Constant region-specific PCR were conducted to investigate how the positive sequences we got arranged using several different primers. The PCR products indicated that the segments S3, S2 and S4 aligned in the lambda light constant region and span about 15kb distance.
Several biochemical parameters, the most important of which are total IgG, kappa and lambda light chain distribution and thin layer isoelectric focusing, were determined in the cerebrospinal fluid of 36 multiple sclerosis patients.
Amyloid fibrils were labelled in six of seven cases: in four cases with anti-transthyretin (TTR) antibodies and in two with anti-lambda light chain antibodies.
Protein electrophoresis revealed the IgA lambda paraprotein (40.5?g/l) and she had a Bence Jones (BJ) proteinuria of the lambda light chain type.
We studied a 58-year-old woman with severe therapy-refractory hyperlipidemia, xanthomatosis, and multiple myeloma (immunoglobulin A, lambda light chain).
Evidence for the genomic organization of human lambda light chain joining (J) region gene segments is presented.