外显子剪接 alternative splicing(3)   alternative splicing      Proof of ERCC1 gene exon VIII alternative splicing and its function study      卵巢癌细胞ERCC1基因VIII外显子剪接变异及功能研究 短句来源      Based on homology analysis and RT-PCR, a novel isoform of human TCP11 gene was isolated. It encodes a 503 amino acid protein that is highly homologous to the mouse 566 amino acid protein Tcp-11. Tcp-11 is important to sperm function because it may be the receptor of fertilization promoting peptide (FPP). Complicated alternative splicing was found to exist between TCP11a and TCP11b genes.      运用同源比较和PCR法 ,从人睾丸组织中分离了人受精促进肽受体TCP11基因的一个新的剪切体TCP11b ,它编码 5 0 3个氨基酸的蛋白质 ,与TCP11a相比 ,在基因组的 5′端存在复杂的外显子剪接现象。 短句来源      Objective To prove the validity of exon VIII alternative splicing of ERCC1 and study its function on the expression of ERCC1 gene and the role in the development of platinum based anticancer drug resistance.      目的　确认卵巢癌细胞ERCC1基因VIII外显子剪接变异的存在 ,探讨该剪接变异对ERCC1基因表达的作用及其对卵巢癌细胞对铂类药物耐药性的影响。 短句来源   “外显子剪接”译为未确定词的双语例句      Mutations in MYBPC3 were found in 2 pedigrees, 1 with complex mutation (Arg502Trp and splicing mutation IVS27+12C>T) and 1 with novel frame shift mutation (Gly347fs) and the latter pedigree has sudden death history.      2个家系MYBPC3基因发生错义突变、剪接突变和移码突变,1个家系先证者为复合突变即18外显子错义突变Arg502Trp及27外显子剪接突变即IVS27+12C>T,先证者之母携带错义突变,先证者之父携带剪接突变; 在另一家系首次发现Gly347fs移码突变,该家系中1例猝死。 短句来源      The human cepo-A and cepo-B are composed of 4 and 5 exons respectively, the sequences of the intron/exon junctions were all exactly consistent with the typical GT/AG consensus motif of the splice donor and acceptor sites.      cepo—A由4个外显子组成、cepo—B由5个外显子组成； cepo-A和cepo—B的所有内含子／外显子剪接边界均符合典型的GT／AG剪接模式。 短句来源      Based their sequence, we conclude that the cDNA cloned in our lab represents 4-kb isoform, the alternative poly(A) tailing cause 2-kb isoform, another exon splicing new transcript cause the 4.2-kb form, and we confimed 6-exons transcript by PCR amplification in human tissues including liver.      序列特征分析表明，我们克隆的序列为4-kb转录本，2-kb转录本是由不同的加尾信号剪接形成的，4.2-kb转录本可能是由原来的序列前面多剪接了一个外显子形成的。 通过特异引物的方法从人肝等组织中验证了这个6个外显子剪接本的存在。 短句来源      DNA sequencing and genomic DNA walking were used to identify the primary structure features and exons.      用DNA序列测定与DNA步移实验，分析基因一级结构及外显子剪接。 短句来源      1587-1588 ins T involved a single base insertion be- tween nucleotides 1,587 and 1,588 in exon 9, and was predicted to result in frame shift and premature termination.      1587~1588insT2个新突变。 前者属于单碱基替换,位于内含子6的3'端剪接位点,导致跨外显子剪接; 短句来源 更多          相似匹配句对      The Final Cut      最终剪接 短句来源      A presumed missense mutation of RPGR causes abnormal RNA splicing with exon skip ping      PRGR基因突变错义导致外显子跳跃性RNA剪接异常 短句来源      Proof of ERCC1 gene exon VIII alternative splicing and its function study      卵巢癌细胞ERCC1基因VIII外显子剪接变异及功能研究 短句来源      Splicing of Interrupted Genes in Eukaryotes      间断基因的剪接 短句来源      According the research, we find that the RET gene isocated in the centromeric region of chromosome 10q11.2, and consists of 20 exons .      它具有20个外显子。 短句来源 查询“外显子剪接”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   alternative splicing The presence of two fractions in the mRNA-18°C indicates alternative splicing. 例句来源       This supports tissue-specific regulation of polι gene expression through alternative splicing. 例句来源       The contributions of alternative promoter usage, alternative splicing, and mRNA editing to transcript diversity in eukaryotic cells are evaluated. 例句来源       This isoform differs from oct-1R, in particular, by the structure of 5"-terminal exon, which is the result of alternative splicing. 例句来源       A product of alternative splicing contained an open reading frame coding for the cytoplasmic portion of LTβ. 例句来源       更多                    Aim To clone LVA calcium channel (also known as T-type calcium channel) from INS-1 cell line derived from rat pancreatic β cells. Methods RT-PCR, 3'-RACE and 5'-RACE were used to clone coding sequence of the whole gene. DNA sequencing and genomic DNA walking were used to identify the primary structure features and exons. Results The cloned gene, named as α1 G-INS, was composed of 6864 bp, encoding 2 288 amino acids, which shares 96. 3% identity to α1G, the neuronal T-type calcium channel. Compared to α1G, ...             目的从大鼠胰腺β细胞中，克隆ＬＶＡ钙通道（即Ｔ－型钙通道）基因，并鉴定其一级结构的特征。方法用ＲＴ－ＰＣＲ，３’－ＲＡＣＥ及５’－ＲＡＣＥ法，从总ＲＮＡ中克隆Ｔ－型钙通道全长基因；用ＤＮＡ序列测定与ＤＮＡ步移实验，分析基因一级结构及外显子剪接。结果克隆了Ｔ－型钙通道的全长结构基因，命名为α１Ｇ－ＩＮＳ。该基因由６８６４个碱基组成，编码２２８８个氨基酸，与从神经组织中克隆的钙通道α１Ｇ相比较：在氨基酸水平上有９６．３％的同源性，在４个结构域的跨膜区内的氨基酸及Ⅰ、Ⅱ结构域间的胞内环链部分的氨基酸具有高度保守性；两者间具有３个明显不同的结构区段，基因组ＤＮＡ步移法分析表明，这种差异是由于ｍＲＮＡ前体的不同剪接所致。此外，尚有１０个氨基酸替代散在于分子内其它区域，这种替代是由于基因突变造成的。结论成功地从大鼠胰腺β细胞中，克隆到ＬＶＡ钙通道基因中的１个新亚型（ｉｓｏｆｏｒｍ）α１Ｇ－ＩＮＳ，对深入研究钙离子所涉及的许多重要的基本生命过程有着重要的意义。 文摘来源          Based on homology analysis and RT-PCR, a novel isoform of human TCP11 gene was isolated. It encodes a 503 amino acid protein that is highly homologous to the mouse 566 amino acid protein Tcp-11. Tcp-11 is important to sperm function because it may be the receptor of fertilization promoting peptide (FPP). Complicated alternative splicing was found to exist between TCP11a and TCP11b genes. The gene has been mapped to human chromosome band 6p21 by fluorescence in situ hybridization. Results of Northern blot an...             运用同源比较和PCR法 ,从人睾丸组织中分离了人受精促进肽受体TCP11基因的一个新的剪切体TCP11b ,它编码 5 0 3个氨基酸的蛋白质 ,与TCP11a相比 ,在基因组的 5′端存在复杂的外显子剪接现象。运用荧光原位杂交 (FISH)方法 ,显示该基因定位到人染色体 6p2 1。Northern杂交及多组织RT PCR的结果显示该转录本在正常睾丸中表达 ,而其他组织、无精症患者及胎儿睾丸组织中未见该基因的表达。该结果结合mTcp 11功能的提示 ,TCP11b这种转录本对精子发生和人受精过程可能起重要作用。 文摘来源          Objective To identify a novel isoform of hTCPll gene and investigate its expression and alternative splicing. Methods According to the sequence of human ESTs which are highly homologous to hTCPlla, primers for PCR were synthesized. Then, the amplified fragments were cloned and sequenced; some methods including BLAST, ClustalW and RT-PCR were used for genomic analysis, study of alternative splicing and gene expression among multiple tissues and different testis tissues. Results A novel isoform of hTCPll gene...             目的 分离、克隆人受精促进肽受体TCPll基因1个新的转录本TCPllc的全长cDNA,研究其表达的阶段性、组织特异性及TCPll基因3种转录本的剪接方式。方法 运用BLAST方法获得与TCPlla同源的ESTs,以逆转录的人睾丸cDNA为模板进行PCR扩增,将PCR扩增片段克隆入pGEM-T easy载体并测序;采用BLAST、ClustalW及RT-PCR方法分析各转录本的基因组结构及剪接方式;RT-PCR方法分析其组织表达的特异性和阶段性。结果 获得1个新的全长cDNA,它编码440个氨基酸的蛋白质,与TCPlla、b相比,在基因组的5′-端存在复杂的外显子剪接现象。RT-PCR结果显示该转录本在正常睾丸中表达,而其他组织、无精症患者及胎儿睾丸组织中未见该基因表达。结论 从人睾丸组织中分离到人受精促进肽受体TCPll基因1个新的转录本TCPllc, 结合mTcp-11的功能提示,TCPll基因a、b、c这3种转录本对精子发生和人受精过程可能起重要作用。 文摘来源   << 更多相关文摘         相关查询: 剪接 外显子 外显子7 选择剪接 剪接异构体 可变性剪接 选择性剪接 剪接法 外显子拼接 变位剪接 剪接变异 剪接位点  对查询结果不满意

CNKI小工具 在Springer中查有关的英文论文 在知识搜索中查有关外显子剪接的内容 在数字搜索中查有关外显子剪接的内容 在概念知识元中查有关外显子剪接的内容 在学术趋势中查有关外显子剪接 的内容

CNKI主页 |  设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务

2008 CNKI－中国知网

京ICP证040431号 互联网出版许可证 新出网证(京)字008号 北京市公安局海淀分局 备案号：110 1081725  2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社