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In this report,the soluble antigens prepared from Plasmodium knowlesiand P.vivax were used in the indirect hemagglutination test on the patientsinfected with P.falciparum and P. vivax correspondingly. 69 out of the 111 confirmed cases (62.2%) of P. falciparum showed positive reactions. Theresults of testing sera from persons without malaria infection were 9.2%.To ascertain whether any relationship might be found between the positivereactions and the clinical appearance of the patients with P. falciparum,the... In this report,the soluble antigens prepared from Plasmodium knowlesiand P.vivax were used in the indirect hemagglutination test on the patientsinfected with P.falciparum and P. vivax correspondingly. 69 out of the 111 confirmed cases (62.2%) of P. falciparum showed positive reactions. Theresults of testing sera from persons without malaria infection were 9.2%.To ascertain whether any relationship might be found between the positivereactions and the clinical appearance of the patients with P. falciparum,the cases were divided into 3 groups as follows.Group I consisted of casesshowing severe or critical clinical manifestations, all of them (100%)showed positive reactions by the indirect hemagglutination test; GroupII consisted of 88 cases showing moderate and mild symptoms; 12 asympto-matic carriers belonged to Group III. Only 51 out of 88 (58.0%) werefound to give positive reactions in Group II, and 7 out of 12 (58.3%)showed positive reactions in Group III. Obviously, the positive reactionsin Group I was significantly high.than those in Group II and GroupIII (0.05>P>0.01). Probably, this difference was related to the degreeof parasitemia.Follow--up observations were made on 21 of the 111 cases. Sera ofthese cases were examined 20--30 days after treatment as well as duringthe acute stage; the results showed no difference.Patients infected with P. vivax were also examined by the indirecthemagglutination test and 50% positive reaction was obtained.Serum IgM values were determined for 106 patients with P. vivax infec-tion. It was shown that the values were significantly higher than those ofnormal persons, i.e., md of IgM value of the patients = 1.47, md of IgMvalue of the norml persons = 1.16, U = 3.835>2.58,P<0.01. IgG values insera from 73 cases of P. vivax were determined and the results showedthat they were significantly higher than those in normal persons too,i.e., md of IgG value of the patients = 13.58, md of IgG value of normalpersons = 12.06, U = 3.81>2.58,P<0.01. 本文报导用诺氏疟原虫制备的可溶性抗原以间接血凝试验检测111例恶性疟患者,阳性率为62.2%。正常人对照阳性率为9.2%。用间日疟原虫制备的抗原检测间日疟病人阳性率为50%。对间日疟患者进行IgM(106例)、IgG(73例)含量测定,结果均较正常人为高。 Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method of purification... Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method of purification of the CoF was modified in our laboratory by using slab polyacrylamide gel electrophoresis which is a simple method and CoF of purer quality can be obtained. The method of the hemolysis test of Kabakci was also modified and simpified. Only 0.1 ml blood from the ear lobe is taken for the test, compatible serum is used as a source of complement and purified CoF solution is added and result can be read in one hour. The results obtained with this method are in agreement with those using Kabakci method.Since 1978 the CoF hemolysis test was performed in normal individuals, PNH patients and cases of various types of anemia. The percentage of hemolysis in 22 normal individuals was 0-5.6%, of these the value was below 4% in 19 cases, the mean value was 2.4%. The percentage of hemolysis in 21 cases of PNH patiants was 7.0-69.0% with a mean value of 27.9±2.3%. of 8 cases suspicious of PNH the CoF hemolysis was normal in 5 cases and slightly increased in 3 cases, with a mean value of 5.4±0.72%.The result of our studies shows the CoF hemolysis test completely in agreement with the sucrose water test, and is more sensitive than the acidified serum hemolysis test. Therefore, the CoF hemolysis test is considered to be a useful test for the diagnosis of PNH. 作者对Kabakci提出的蛇毒因子溶血试验进行了改进,并利用改进法对正常人、临床诊断为阵发性睡眠性血红蛋白尿症患者,疑似该症以及其它贫血患者做了对比检查。检查结果表明,本试验与糖水试验完全一致,比酸化血清溶血试验敏感,与红细胞乙酰胆碱酯酶活性的降低大多平行。作者认为改进的蛇毒因子溶血试验可做为阵发性睡眠性血红蛋白尿症的有效诊断方法之一。 The active rosette test was adapted as in vitro assay and researched for relation withhuman tumor antigens——gastric cancerous, lung cancerous and hepatic cancerous. Peripheral lymphocytes were purified from 114 cancer patients, 48 noncancer patients and 30 normal adults. Tumor antigen, incubated with lymphocytes from a corresponding cancer patient, produced a significant increase in the ability of the lymphocytes to form active rosette-forming cells (A-RFG) when compared with lymphocytes cultured without antigen... The active rosette test was adapted as in vitro assay and researched for relation withhuman tumor antigens——gastric cancerous, lung cancerous and hepatic cancerous. Peripheral lymphocytes were purified from 114 cancer patients, 48 noncancer patients and 30 normal adults. Tumor antigen, incubated with lymphocytes from a corresponding cancer patient, produced a significant increase in the ability of the lymphocytes to form active rosette-forming cells (A-RFG) when compared with lymphocytes cultured without antigen (p∠0.001). This increasing effect on A-RFC still remained after refrigeration. Tumor antigen incubated with lymphocytes from normal donors produced no increase in their A-RFG. It should be noted that this assay appears in a certain manner to be specific for the antigen to which the individual is corresponding cancer patient, for example, gastric cancerous antigen activated A-RFG of a gastric cancerous patient, but produced no increase in breast patient. This assay provides a new in vitro method for investigating mechanism for cell-mediated immunity and also a new specific diagnostic method for cancer. 在试管内研究人类肿瘤抗原-胃癌、肺癌和肝癌抗原——与活性玫瑰花形成试验的关系。由114例肿瘤病。48例非肿瘤病人和30例正常人的血液标本中分离淋巴细胞。肿瘤抗原与相应的癌患者的淋巴细胞温育后,淋巴细胞形成活性玫瑰花的百分率比未加抗原的对照组显著增加(P<0.001)。与抗原温育过的淋巴细胞经冷藏后仍保留这种激活效应。肿瘤抗原与正常人的淋巴细胞温育过不产生活性玫瑰花细胞的增多效应。值得注意的是,这种测定具有一定特异性,例如胃癌抗原能激活胃癌病人的活性玫瑰花细胞而对乳癌病人无激活效应。这一实验可做为研究细胞免疫机制的新的体外试验方法,并为肿瘤的特异性诊断提供新的方法。
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