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    TGFβ1 Expression in Tumor Tissue and Body Fluid of TCC Patients and Its Effects on Topical and Systemic Cellular Immunity
    膀胱癌患者体液和肿瘤组织内TGFβ1水平及其对肿瘤局部和全身细胞免疫功能影响的研究
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    Studies on Oncogene and Tumor Suppressor Gene in the Plasma and the Tumor Tissue from Pancreatic Cancer Patients
    胰腺癌患者肿瘤组织及外周血浆中癌基因和抑癌基因的研究
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    Experimental Studies and Clinical Significance of Bone Marrow Angiogenesis and Related Mechanism in Adult Patients with Acute Leukemia
    成人急性白血病患者骨髓血管新生及相关机理的实验研究和临床意义
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    Multidisciplinary Therapy and Laboratory Study after Radical Surgery in High-Risk Cervical Cancer Patients
    高危宫颈癌患者根治术后综合治疗和实验研究
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    Study of the Enhancing Effect of Rbc Component Transfusion on EⅡAF of Patients with Digestive Tract Malignancy
    红细胞成分输血促进消化道肿瘤患者EⅡAF的实验研究
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    Effect of NSCLC Expressing FasL on TILs and Detection of sFas and sFasL in Serum
    NSCLC表达FasL对TIL的影响及患者血清中可溶性Fas、FasL的检测
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    Significance of Osteopontin Overexpression in Hepatocellular Carcinoma with Liver Transplantation Mcl-1 Antisense Therapy Chemosensitizes Cholangiocarcinoma in Vitro Research
    骨桥蛋白在肝癌肝移植患者过度表达与临床特征相关性研究及Mcl-1反义寡核苷酸治疗增加胆管细胞癌对化疗药物敏感性的研究
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    Study on the Mitochondrial DNA Mutations in Tumor Tissues and Peripheral Lymphocytes of Laryngeal Squamous Cell Carcinoma
    喉鳞状细胞癌患者组织及血液中线粒体DNA突变的研究
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    1.The Expression of Human Macrophage Metalloelastase in Gastric Cancer Cell Lines and Gastric Cancer Tissues and Its Clinical Significance 2.The Study of the Associalation between DPYD Gene Polymorphism and Metabolism of 5-FU and the Clinical Signifi
    1.人巨噬细胞金属弹性蛋白酶在胃癌细胞及胃癌组织中的表达及临床意义 2.胃癌和结肠癌患者二氢嘧啶脱氢酶基因多态性与5-FU代谢及临床意义探索
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    Cyclin D1、ERCC1 Gene Polymorphism and Susceptibility to Childhood Acute Lymphoblastic Leukemia in a Chinese Population
    中国儿童急性淋巴细胞白血病患者Cyclin D1、ERCC1基因多态性的研究
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  patients
Genomic DNA was extracted from blood samples of healthy controls and from patients with HBV infection.
      
The immunoassay was used to check the BALP of the blood serum that was from 42 primary osteosarcoma patients.
      
The patients were followed up from five months to 49 months with an average of 24.3 months.
      
Detection of abnormal myocardial perfusion is crucial to the prognosis of patients with coronary artery disease (CAD) after they have undergone percutaneous coronary intervention (PCI).
      
For those patients with acute or total occlusion, the levels of myocardial perfusion before and after PCI were similar, as determined by IC-MCE and visually analyzed from 61 segments (P >amp;lt; 0.05).
      
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The changes of tyrosine transaminase activity (TAT) and nucleotide 5'-phosphodiesterase activity (5'-NPDase) during foetal development and carcinogenesis in rats and in human beings were observed. Results so far obtained indicate that (1) a higher positive rate of the fastest moving isoenzyme band for 5'-NPDase has been noted in AFP (-) patients with liver cancer, and (2) TAT, 5'-NPDase and AFP may be taken together as good markers for the study of gene expression during liver caroinogenesis and foetal development....

The changes of tyrosine transaminase activity (TAT) and nucleotide 5'-phosphodiesterase activity (5'-NPDase) during foetal development and carcinogenesis in rats and in human beings were observed. Results so far obtained indicate that (1) a higher positive rate of the fastest moving isoenzyme band for 5'-NPDase has been noted in AFP (-) patients with liver cancer, and (2) TAT, 5'-NPDase and AFP may be taken together as good markers for the study of gene expression during liver caroinogenesis and foetal development.

本文测定了大鼠及人体胚胎发育和癌变过程中TAT和5′-NPDase活力变化,所得结果表明: (一)5′-NPDase快速同工酶区带在甲胎蛋白(AFP)阴性肝癌患者血清中阳性率较高。(二)TAT,5′-NPDase和AFP也许可作为研究癌、胚以及肝癌癌变过程中基因表达的三个互补生化指标。

A new oncofetal antigen on the plasma membrane of human hepatoma cells hasbeen detected and partially purified.Human hepatoma tissues obtained from partialhepatectomy were used to prepare plasma membrane by sucrose density gradientcentrifugation.The antiserum to this plasma membrane was absorbed by normalliver powder prepared from the normal portion of the liver of the same patient.Thisabsorbed antiserum gave a positive reaction with the soluble products of the hepatomacell plasma membrane in double immunodiffusion...

A new oncofetal antigen on the plasma membrane of human hepatoma cells hasbeen detected and partially purified.Human hepatoma tissues obtained from partialhepatectomy were used to prepare plasma membrane by sucrose density gradientcentrifugation.The antiserum to this plasma membrane was absorbed by normalliver powder prepared from the normal portion of the liver of the same patient.Thisabsorbed antiserum gave a positive reaction with the soluble products of the hepatomacell plasma membrane in double immunodiffusion and counter-current immuno-electrophoresis,but a negative reaction with those of the normal liver cell plasmamembrane.With the immunofluoresccent method,it is demonstrated that thisabsorbed antiserum reacts specifically with hepatoma cells and the 7402 cell line(ahuman hepatoma cell culture line).It may be surmised therefore that there is anantigen on the plasma membrane of the hepatoma cells but absent from normal livercells.Further studies indicated this membranous antigen to be of the oncofetal type.The plasma membrane of human embryonic liver(3~4 months old)also possesses thisantigen.We tried to purify this antigen from embryonic liver by means ofimmunoaffinity chromatography.A protein eluated from the column with thesedimentation coefficient of 0.33~0.45 exhibits specific affinity for antihepatomaserum.

在人肝癌细胞膜中发现了一个新的癌胚抗原,并且进行了部分纯化。从部分肝切除术取人肝癌组织,用蔗糖密度梯度超离心法分离细胞膜,以此细胞膜制备的抗血清,经患者本人正常肝粉吸收,吸收后的抗血清与肝癌细胞膜溶解物作双向免疫扩散和对流免疫电泳均呈阳性反应,而与正常肝细胞膜则均呈阴性。用免疫萤光法鉴定,证明该血清能特异性地作用于肝癌细胞膜和7402人肝癌细胞培养株的细胞膜。因此,我们认为:人肝癌细胞膜具有不存在于正常肝细胞的抗原。进一步研究证明,这种抗原也存在于3~4个月人胚胎肝细胞膜中,显示这是一种癌胚抗原。我们取人胚胎肝组织,用免疫亲和层析法尝试了这种膜抗原的纯化工作,从亲和层析柱流下的蛋白质,其沉降系数为0.33~0.45,并对上述吸收后的抗血清显示特异性的亲和力。

In this article, we described a procedure of isolation of basic proteins from brain glioma and stomach cancer, and presented the results of detecting tumor antigenicity by means of a macrophage electrophoretic mobility (MEM) test with lymphocytes isolated from 623 subjects,which consisted of 95 normal persons, 167 cases of brain tumor 65 cases of stomach cancer, 161 cases of miscellaneous malignant tumor, 25 cases of benign tumor and 120 cases suffering from other diseases.Using"glioma basic protein" (GBP) as...

In this article, we described a procedure of isolation of basic proteins from brain glioma and stomach cancer, and presented the results of detecting tumor antigenicity by means of a macrophage electrophoretic mobility (MEM) test with lymphocytes isolated from 623 subjects,which consisted of 95 normal persons, 167 cases of brain tumor 65 cases of stomach cancer, 161 cases of miscellaneous malignant tumor, 25 cases of benign tumor and 120 cases suffering from other diseases.Using"glioma basic protein" (GBP) as an antigen, the incidence of positive reactions in the MEM test was 1.2% (1/85) in normal persons, 92 % (145/158) in brain tumors, 94% (16/17) in stomach cancers,91.1% (92/101) in miscellaneous malignancies, 20% (5/25) in benign tumors and 9.1% (8/88) in the rest cases. When using "stomach cancer basic protein"(SBP)as antigen,the positive incidence was found as follows :0% in 10 normal persons, 89% (8/9) in brain tumors, 95% (36/38) in stomach cancers, 92% (55/60) in miscellaneous malignancies and 6.3% (2/32) in the others.The above results demonstrated that more than 90% lymphocytes from patients with brain tumor, stomach cancer, or miscellaneous malignancies, when allowed to react with GBP or SBP, displayed a slowing effect on MEM while that from normal persons or non-tumor patients more than 90% did not show such an effect. Our results are broadly in accord with the observations of Field et al but contrary to that of Forrester et al.

本文介绍了从人脑胶质瘤和胃癌中提取硷性蛋白的方法,并用总数为623人的淋巴细胞(包括健康人95人、脑瘤167例、胃癌55例、其他部位癌肿161例、良性肿瘤25例以及非肿瘤性疾病120例),对以上两蛋白制剂的肿瘤抗原性进行巨噬细胞电泳试验(MEM)的检查。 用脑胶质瘤硷性蛋白(GBP)作为抗原时,MEM试验的阳性率为:正常人为1.2%(1/85)、脑瘤为92%(145/158)、胃癌为94%(16/17),其他部位癌症为91.1%(92/101)、非肿瘤性疾病为9.1%(8/88)、良性肿瘤为20%(5/25)。当用胃癌硷性蛋白(SBP)作为抗原时,其阳性率为:正常人为0%(0/10)、脑瘤为89(8/9)、胃癌为95%(36/38),其他部位癌症为92%(55/60)、非肿瘤性疾病为6.3%(2/32)。 上述结果表明90%以上脑瘤、胃癌及其他部位癌症病人的淋巴细胞,当接触GBP或SBP时,有使巨噬细胞电泳减缓的作用;而在相同条件下90%以上的正常人及非肿瘤性疾病患者无此作用。以上结果初步看来与Field等人所观察到的结果相一致,而与Forres-ter等人的结果却相反。

 
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