As compared with the control group,in liver tissue,ratio of protein content (experimental group/control group) is 0. 60: 1. 00,ratio of DNA content is 0. 49: 1. 00;
Moreover,MDA contents in liver tissue were higher in LPS group than control and berberine treatment group,but there were no significant difference in SOD activity between berberine treatment and LPS group.
Results\ The relative mRNA expression of α 1a and α 1b AR subtype in liver tissues of cirrhotic patients (0.26±0.12,0.03±0.01) was significantly lower than that of controls (0.86±0.38, 0.23±0.10, P <0.01).
Methods: Mouse PD-L1 cDNA was amplified by RT-PCR from total RNA of mouse liver tissues and was cloned into shuttle vector pSNAV1; the products were then transferred into BHK21 cells by lipofectamine and rAAV2-PD-L1 was screened out.
Conclusion CYP2D1 mRNA expression level in hepatocarcinogenesis rat liver tissues is same to in normal rat ,CYP2D1 mRNA also can express in brain tissues and its level is lower in liver tissues.
Methods The total RNA was extracted from rat hepatic tissue. CYP2J3 gene was amplified by RT-PCR and OE-PCR,and cloned into the pcDNA 3.1(+) to form an eukaryotic expression vector.
The expression of adipose tissue aromatase and blood E 2 level were significantly higher in OVX+EA+T group than in OVX+EA group (P< 0.05), but there was no significant change in hepatic tissue aromatase.
④Activities of CYP1A1 (7-ethoxyl isoxazole O-deethylase,EROD), CYP2E1 (aniline hydroxylase, ANH) and CYP3Al(erythromycin N-demethylase,ERD) in rat hepatic tissue using enzymatic methods;
Hepatic tissue (0.1g) was procured before warm ischemia and 0.5,1,2 and 3 h after reperfusion in WI group,before and after IPC and 0.5,1,2 and 3 h after reperfusion in IPC group. The expression of eNOS and iNOS mRNA was detected by fluorescence-quantitating-PCR.
For the MDA concentration, it decreased in hepatocarcinoma tissue following ischemia and reperfusion until 7d(21.59±0.59). In contrast, the increasing of that in normal hepatic tissues was marked after reperfusion for 0 min to 7d(29.04±1.43) and kept at higher level than that before ischemia (18.26±0.43).
In the experimental group, the postive rate of CD44V6 in the gastric and ovaria n tissues were 63.2% and 42.1%, In the control group, the postive rate of CD44V6 in the gastric and hepatic tissues were 70% and 60%, the two groups has no obv ious difference(P>0.05).
In addition to the examination of Superoxide Dismutase (SOD) and malondialdehyde (MDA), the apoptotic changes in the hepatocarcinoma and its contiguous area and normal hepatic tissues were observed by HE staining.
METHODS: The experiment was performed at the Laboratory of Cardiology, Tongji Hospital from September 2004 to February 2005. Total RNA was extracted from hepatic tissues of rats to form CYP2J3CDNA by reverse transcription reaction and obtain A and B fragments of CYP2J3 gene, totally 1 228 bp and 345 bp, respectively by polymerase chain reaction (PCR) amplification.
[WT5HZ]Results[WT5”FZ] The relative mRNA expression of α 1a adrenoceptor subtype in the hepatic tissues was significantly lower in the cirrhotic patients (0 26±0 12) than in the controls(0 86±0 38, P <0 01).
Antioxidant activity of melatonin and some new melatonin analogue indole derivatives were investigated, using lipid peroxidation and superoxide anion radical scavenger activity assays, in rat liver tissue homogenate.
Development of liver pathology was accompanied by adipose dystrophy, fibrosis, and an increase of triglycerides and lipid peroxidation products in the liver tissue.
The protein expression profiles of liver tissues were acquired by fluorescence two-dimensional difference gel electrophoresis (2D DIGE) that incorporated a pooled internal standard and reverse fluorescent labeling method.
Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR.
The overall amounts of both phospholipids and gangliosides increased appreciably in the subcutaneously growing hepatoma (in contrast to the intrahepatically growing tumor) in comparison to the control hepatic tissue.
The rate of metabolism of testosterone in histologically normal hepatic tissue was as high as in cirrhotic tissue, while the rate of metabolism in fatty liver was lower.
The main pathogenetic factor is obviously the functioning hepatic tissue, reflected by the galactose elimination capacity (r=0.666;P>amp;lt;0.01; theophylline clearance vs galactose elimination capacity).
A single dose of 30 mg/kg (～30% LD50) endosulfan significantly (p>amp;lt;0.001) increased the TBARS and, hence, the lipid peroxidation in cerebral and hepatic tissues of rats.
The same doses caused a significant alteration in glutathione redox status of cerebral and hepatic tissues, where total glutathione and oxidized glutathione were measured by an enzymatic cycling procedure.
肝脏组织
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